Jillian E Guertin, Joao de Agostini Losano, Sophia Salazar, Justin Callaham, Bradford W Daigneault
{"title":"Prolonged maintenance of stallion semen by optimization of cooling conditions.","authors":"Jillian E Guertin, Joao de Agostini Losano, Sophia Salazar, Justin Callaham, Bradford W Daigneault","doi":"10.1016/j.jevs.2024.105243","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105243","url":null,"abstract":"<p><p>Bottlenecks to the success of equine assisted reproductive technologies (ART) include suboptimal conditions for prolonged storage of stallion sperm. Shipped stallion sperm are transported in cooling devices designed to maintain temperature for up to 48 h. Increasing the storage time of cooled semen while maintaining acceptable motility would relieve logistical ART challenges. Experiments were conducted to test the hypothesis that external regulation of shipment container temperature would prolong storage time of cooled stallion semen. Initial experiments determined the effect of pre-freezing cooling cans at -20°C or -80°C on sperm motility. Fresh sperm was extended in INRA96 and placed in commercial Equitainers for 3.5 days (84 h). Quantification of sperm kinematics was determined every 12 h. Sperm held in Equitainers with -20°C cans maintained higher total and progressive motility than -80°C conditions at 60 h (63%, 29% vs. 32%, 17%, respectively). Internal monitoring of Equitainers containing -20°C freezer can temperature identified 20°C as the threshold for rapidly decreased motility. In the second experiment, sperm were maintained in Equitainers containing -20°C freezer cans and placed in two different conditions: 1) ambient temperature for 48 h (23°C) or 2) 5°C for external temperature regulation up to 8 days, or when total motility dropped below 50%. Sperm kinematics was determined every 24 h. Total motility from samples held with external temperature regulation (5°C) remained above 50% for more than 7 days. These findings suggest that cooled stallion semen can be advanced beyond traditional 48 h constraints by improving temperature maintenance of storage conditions.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105243"},"PeriodicalIF":1.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Denyus Augusto de Oliveira Padilha, Suelen Fernandes Padilha, Rafaela Martins, Bruna Elisa Machado Scheffer, Mayumi Regina Miliorini, Laila Talarico Dias, Rodrigo de Almeida Teixeira
{"title":"Estimation of Genetic Parameters for Racing Time and Ranking in Thoroughbred Horses.","authors":"Denyus Augusto de Oliveira Padilha, Suelen Fernandes Padilha, Rafaela Martins, Bruna Elisa Machado Scheffer, Mayumi Regina Miliorini, Laila Talarico Dias, Rodrigo de Almeida Teixeira","doi":"10.1016/j.jevs.2024.105244","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105244","url":null,"abstract":"<p><p>This study aimed to estimate genetic parameters for race time in seconds and final ranking, as well as to analyze the genetic trends associated with race time. The study utilized a dataset consisting of 23,290 records of race times and final ranks at distances of 1,000, 1,600, and 2,000 m from 6,213 Thoroughbred horses from the São Paulo Jockey Club. Our model considered the year of the run, animal sex, race class, track conditions, the linear effect of horse weight and age, and the quadratic effect of age as fixed covariates. Random effects included direct additive genetic, animal permanent environmental, and residual effects. Heritability estimates ranged from 0.01±0.03 to 0.17±0.04 (race time); 0.04±0.03 to 0.09±0.04 (final ranking), and repeatability from 0.19±0.02 to 0.37±0.02 (race time) and 0.15±0.02 to 0.23±0.04 (final ranking). Pearson's correlations of estimated breeding values between race time and ranking ranged from -0,027±0.16 to 0.84±0.01. When analyzing the genetic trend for races at a distance of 1,000 m, we observed a decrease in race time over time. However, for races at 1,600 and 2,000 m, a clear pattern of time reduction was not apparent. Race time offers a more promising response to selection, particularly in shorter races. Selecting for race time is expected to have a greater indirect impact on final rank. Genetic trends have revealed limited genetic progress over the years; therefore, it is advisable to reevaluate the current selection process.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105244"},"PeriodicalIF":1.3,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R W Springer, N M Cherry, J P Muir, R H Raub, K B Wellmann, T N Jones
{"title":"Fiber composition, in vitro true digestibility, and fiber degradation of hempseed meal compared to mainstream feedstuffs using equine feces as microbial inoculum within the Daisy<sup>II</sup> Incubator.","authors":"R W Springer, N M Cherry, J P Muir, R H Raub, K B Wellmann, T N Jones","doi":"10.1016/j.jevs.2024.105230","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105230","url":null,"abstract":"<p><p>Recent changes in legislation have put hempseed meal (Cannabis sativa L.; HSM) under consideration as a potential livestock feedstuff. Digestibility of HSM has been researched in other species, however, there are little data in horses. The study objective was to determine the in vitro true digestibility (IVTD) of HSM compared to common equine feedstuffs. An in vitro split-split plot design used four geldings (8.5 ± 3.9yr; 558.4 ± 31.0kg; 6.0 ± 0.4 BCS) to investigate the IVTD of HSM compared to alfalfa hay (ALF), Coastal Bermudagrass hay (CBH), soybean meal (SBM), rice bran pellets (RBP), and a commercial concentrate (Bluebonnet Equilene Pellets; EQU). Fresh feces were collected after a 21-d acclimation period and used as incubation inoculum. Dried ground feed samples were incubated in ANKOM filter bags within 2 DaisyII incubators for 48h. Split samples were analyzed for pre-fermentation neutral detergent fiber (NDF). Incubated filter bags were dried and analyzed for NDF to determine IVTD and fiber degradation. Hempseed meal NDF was greater than ALF (P<0.001), RBP (P<0.001), and SBM (P<0.001) but less than CBH (P<0.001) and tended to be greater than EQU (P=0.077). The IVTD of HSM was greater than CBH (P<0.001) but less than all other feedstuffs (P<0.001). The results indicate that HSM has an NDF and IVTD between ALF and CBH. Due to its lower digestibility and NDF content compared to concentrates, HSM has potential to be used as a fiber source in equine rations.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105230"},"PeriodicalIF":1.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Lanci , N. Ellero , C. Castagnetti , F. Perina , F. Dondi , J. Mariella
{"title":"Plasma alpha-fetoprotein in neonatal foals affected by prematurity, sepsis and neonatal encephalopathy","authors":"A. Lanci , N. Ellero , C. Castagnetti , F. Perina , F. Dondi , J. Mariella","doi":"10.1016/j.jevs.2024.105231","DOIUrl":"10.1016/j.jevs.2024.105231","url":null,"abstract":"<div><div>Alpha-fetoprotein (AFP) concentrations have been reported in healthy foals and proposed as a biomarker of sepsis in foals born from mares with experimentally induced placentitis. This study aimed to describe the diagnostic and prognostic value of plasma AFP in foals spontaneously affected by different diseases. The study included all foals less than 72 h old that were diagnosed with either: (1) prematurity (PRE), when born prior to 320 days of gestation with immature physical characteristics; (2) sepsis (SEP), in the presence of both positive blood culture and SIRS or (3) neonatal encephalopathy (NE), with evidence of hypoxic-ischemic injury. Data from healthy foals (H; <em>n</em>=20) were obtained from a previous study. Foals received a complete physical and hematochemical evaluation and blood culture sample collection at hospital admission. Forty-six foals with an average age of 16 h were enrolled and divided into: PRE group (<em>n</em>=7); SEP group (<em>n</em>=14); NE group (<em>n</em>=25). AFP was measured in plasma collected at admission using a commercially available immunoassay validated for horses. AFP was increased in foals in PRE, SEP and NE groups compared with healthy ones (<em>P</em><0.001) but was not able to discriminate between different diseases and outcomes. Overall, AFP was negatively correlated with foal age (<em>r</em>=−0.6; <em>P</em><0.001), foal weight (<em>r</em>=−0.3; <em>P</em>=0.048), monocytes count (<em>r</em>=−0.4; <em>P</em>=0.011) and SAA concentration (<em>r</em>=−0.4; <em>P</em>=0.011). AFP appears to be a useful but non-specific indicator of neonatal health, since it upregulates not only in the presence of SIRS and bacteremia, but also during prematurity and hypoxic-ischemic injury.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105231"},"PeriodicalIF":1.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142639051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genetic characterization of phenotypic traits in endangered Taishu horse breed and their breeding strategy.","authors":"Tomoko Yoshihara, Teruaki Tozaki, Sakura Nakaya, Masaki Takasu, Koki Kawate, Risako Furukawa, Mio Kikuchi, Taichiro Isihge, Shun-Ichi Nagata, Hironaga Kakoi, Seiji Hobo","doi":"10.1016/j.jevs.2024.105233","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105233","url":null,"abstract":"<p><p>The Taishu horse is a native Japanese breed threatened with extinction. It is important to genotype the causative genes of rare phenotypes in endangered breeds because unique genetic traits might be lost unless a breeding strategy for conservation is constructed. In the present study, nine single-nucleotide variants and three indels of nine genes related to coat color, body composition, and gait were genotyped in 56 Taishu horses. Of these, only three genes were polymorphic. The observed coat color phenotypes coincided with the estimated phenotype from the genotypes, whereas black horses showed specific phenotypes that were generally black in winter but turned light brown in summer. No DNA polymorphisms in ligand-dependent nuclear receptor corepressor-like gene and very few polymorphisms in myostatin gene were identified. While means of withers height, chest circumference, cannon circumference, and body length were 122.8, 143.1, 16.9, and 130.2 cm, respectively, no relationship was found between the variants investigated and the body composition measurements. The gaited allele of doublesex and mab-3 related transcription factor 3 gene was not observed and the estimated phenotypes coincided with the observed phenotype, trot. Genotypes of coat color in the Taishu horses are essential for the construction of breeding strategies to conserve black and chestnut horses, which are much less common than bay horses. These results may help breeders construct breeding strategies to conserve rare phenotypic traits in this breed. This initiative will also be beneficial for breeding programs of other native Japanese horses, such as Noma and Miyako, whose numbers are declining.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105233"},"PeriodicalIF":1.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S C Chaintoutis, N Diakakis, Z S Polizopoulou, C I Dovas
{"title":"Serum protein electrophoretic profile changes in West Nile virus-naturally infected horses.","authors":"S C Chaintoutis, N Diakakis, Z S Polizopoulou, C I Dovas","doi":"10.1016/j.jevs.2024.105232","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105232","url":null,"abstract":"<p><p>Serum protein electrophoresis (SPE) is widely used to evaluate protein changes associated with several pathologies, aiming to assist clinical diagnosis. This study aimed to determine, for the first time, SPE profile changes in WNV-naturally infected horses. Sixty horses with different clinical and infection status (encephalitis, asymptomatic infection, and immunologically naïve) were included. Total protein concentrations were determined via an automated biuret method and SPE was performed using a cellulose acetate membrane-based automated system. Statistical analysis revealed significantly higher total protein between both infected horse groups (encephalitis, asymptomatic) and naïve controls. Horses with encephalitis had higher globulin and α2-globulin levels than the other two groups (asymptomatic, naïve), along with lower albumin percentage and albumin-to-globulin ratio. Furthermore, γ-globulin levels were significantly higher in asymptomatically infected compared to control horses. These findings highlight the diagnostic value of SPE profile determination, assisting the clinicians, and providing the grounds for further research.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105232"},"PeriodicalIF":1.3,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Arroyo , L.M. Whitelock , M.E. Stanton , V. Stevenson , L.H. de Aguiar , A. Kelleman
{"title":"Hydroallantois in a mare associated with schistosomus and unilateral ovarian agenesis in the fetus","authors":"E. Arroyo , L.M. Whitelock , M.E. Stanton , V. Stevenson , L.H. de Aguiar , A. Kelleman","doi":"10.1016/j.jevs.2024.105228","DOIUrl":"10.1016/j.jevs.2024.105228","url":null,"abstract":"<div><div>Hydropsical conditions in domestic animals are characterized by abnormal fluid accumulation. While their complete pathophysiology remains unclear, factors such as changes in chorioallantoic membranes, placentitis, or fetal abnormalities may contribute. Hydrops present as a medical emergency that can cause complications for the mare. Early diagnosis and treatment are crucial for the mare's prognosis, as fetal survival is often poor. This report describes a case of hydroallantois in a 9-year-old pregnant Quarter mare at 271 days of gestation, leading to dystocia of a non-viable foal with multiple congenital abnormalities. Given the poor prognosis for fetal survival, transcervical gradual fluid drainage and pregnancy termination were performed. The malformed fetus was subsequently delivered and humanely euthanized.</div><div>Necropsy findings revealed a schistosomus fetus with palatoschisis, a wry nose, prognathism, and unilateral ovarian agenesia. The case underscores the importance of timely intervention in managing hydroallantois. The report contributes to understanding horses' clinical presentation, diagnosis, and management of hydroallantois.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105228"},"PeriodicalIF":1.3,"publicationDate":"2024-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Soon Hon Cheong, Rebecca Franklin-Guild, Laura B Goodman, Yamilka Lago-Alvarez, Yoke Lee Lee, Luis Henrique de Aguiar, Mariana Diel de Amorim, Craig Altier
{"title":"Detection of fungal and bacterial organisms from clinical uterine samples of horses using Next-Generation Sequencing in comparison with culture.","authors":"Soon Hon Cheong, Rebecca Franklin-Guild, Laura B Goodman, Yamilka Lago-Alvarez, Yoke Lee Lee, Luis Henrique de Aguiar, Mariana Diel de Amorim, Craig Altier","doi":"10.1016/j.jevs.2024.105214","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105214","url":null,"abstract":"<p><p>Fungal and bacterial culture is currently the primary method for pathogen detection and identification. Next-generation sequencing is a powerful method for detecting and identifying the presence of microbial DNA in samples. We evaluated the correlation between fungal and bacterial culture with next-generation sequencing in equine uterine samples. Fungal cultures (n=63) were evaluated based on their culture results. In culture positive (n=16) samples, next-generation sequencing identified the same organism in 10 samples (62.5%), 5 samples did not identify fungal agents, and 1 sample identified other species of fungal agents. In no growth samples (n=42), next-generation sequencing did not identify fungal agents in 37 samples (88.1%), 4 samples had a potential fungal pathogen identified, and one sample identified only non-pathogenic fungal organisms. Fungal culture and next-generation sequencing had an 80% agreement and moderate correlation by Kappa coefficient (0.508). Bacterial culture (n=57) was also evaluated based on bacterial culture results. In bacterial culture Positive samples (n=32), next-generation sequencing identified the same organism in 25 (78.1%) samples, and identified different organisms from in the remaining 7 samples. In bacterial no growth samples (n=14), next-generation sequencing detected bacterial presence in 5 samples, and 9 samples had no bacterial DNA identified. The agreement between bacterial culture and next-generation sequencing was 74% with a moderate correlation by Kappa coefficient (0.46). In conclusion, culture and next-generation sequencing have moderate correlation, and next-generation sequencing has the potential as a diagnostic option for enhancing pathogen detection for equine endometritis.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105214"},"PeriodicalIF":1.3,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142622011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Environmental impacts and daily voluntary movement of horses housed in pasture tracks as compared to conventional pasture housing","authors":"Jenna Farmer Long , Jamie Duberstein , Kayla Costin , Todd Callaway , Alexander Abrams , Brooklyne Wassel , Kimberly Toal , Kylee Duberstein","doi":"10.1016/j.jevs.2024.105215","DOIUrl":"10.1016/j.jevs.2024.105215","url":null,"abstract":"<div><div>Pasture tracks are a modern equine housing trend often implemented as a weight control strategy due to the belief that they promote movement, though this is not proven experimentally. To test movement of horses housed in pasture tracks as compared to conventional pasture housing, two experiments were performed using a track (0.2ha, 3.5m in width, 561 m total distance) created around the perimeter of a 2.0 ha pasture. In experiment 1, eight horses were paired and rotated between three treatment locations: track (T), small 2 ha pasture housed within the track (SP), and a larger 20 ha pasture on the same farm (LP). A GPS-equipped watch attached to the crown piece of a halter tracked movement of horses over a ten-week study as they rotated in pairs to each location, with testing in all locations occurring simultaneously. Environmental impacts of housing were evaluated by comparing vegetative biomass and soil relocation throughout the study. In experiment 2, the same horses (n = 8) were rotated in isolated pairs to T or SP, with only one pair of horses tested at a time to ensure that social influences did not confound movement data. Collective results indicate that horses traveled approximately the same distance in T as compared to SP or LP. However, detrimental environmental impacts resulted from the track, including a decrease in vegetative biomass and greater sediment deposition from runoff. Potential environmental impacts should be further investigated to develop best management practices to mitigate erosion caused by pasture tracks.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"143 ","pages":"Article 105215"},"PeriodicalIF":1.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}