{"title":"Development of an ultrasound-guided proximo-medial approach for injection of the carpal flexor tendon sheath in horses and comparison with the conventional proximo-lateral ´blind` technique.","authors":"C A Dörner, V Vargas, C H Castellón","doi":"10.1016/j.jevs.2024.105258","DOIUrl":"https://doi.org/10.1016/j.jevs.2024.105258","url":null,"abstract":"<p><p>The aim of this prospective case-control study was to describe and implement a reliable, accurate, and straightforward ultrasound-guided proximo-medial approach technique for injecting the carpal flexor tendon sheath in horses. The proximo-medial ultrasound-guided approach for intrathecal access to the carpal canal, as described in this study, was firstly developed on ten cadaveric limbs using an undiluted ionic iodinated contrast medium. Landmarks were identified, and the access point was established. Subsequently, the technique was validated in ten horses, and its efficacy was compared with the proximo-lateral ´blind` technique. For each injection, the following was recorded: successful injection (contrast within the carpal canal), number of attempts, and whether a structure other than the carpal sheath was penetrated. The ´blind` technique had a total accuracy of 70% (7/10; 95% confidence interval [CI]: CI 35%-93%), with inadvertent injection into the dorso-palmar pouch of the radiocarpal joint, carpal canal surrounding soft tissue, and intravenous injection being the most common issues associated with this approach. In contrast, the ultrasound-guided approach achieved an accuracy of 100% (10/10; 95% confidence interval [CI]: 69%-100%) for intrasynovial administration of the contrast medium. The ultrasound-guided proximo-medial approach for injecting the carpal sheath is an accurate and technically straightforward technique. This approach should be considered for synoviocentesis of the carpal sheath, particularly in horses with no evident effusion.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105258"},"PeriodicalIF":1.3,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y Nakamae, Y A Elce, M E Saab, J T McClure, A J Doyle
{"title":"Alcohol-based antisepsis without the use of chlorhexidine for arthroscopy in horses.","authors":"Y Nakamae, Y A Elce, M E Saab, J T McClure, A J Doyle","doi":"10.1016/j.jevs.2024.105257","DOIUrl":"10.1016/j.jevs.2024.105257","url":null,"abstract":"<p><p>Alcohol-based antisepsis has shown experimentally to be as effective as 4 % chlorhexidine gluconate (CHG) at reducing bacterial counts (colony forming units; CFU) on equine skin. Our objectives were to determine the immediate and post-surgical reduction in CFU/mL on equine skin prepared with CHG-based or 70 % isopropyl alcohol (IPA)-based (without CHG) protocols in a clinical setting with arthroscopic surgery. Our hypotheses were that the log<sub>10</sub>CFU/mL reduction would not significantly differ between protocols immediately after preparation or at the end of surgery. Six horses underwent a 40 min bilateral tarsocrural joint arthroscopy with each limb randomly assigned to Group A or B. Group A tarsocrural joints underwent a rough scrub using 4 % CHG and a 5 min sterile scrub using 2 % CHG. Group B underwent a rough scrub with neutral soap followed by a 90 s sterile scrub with IPA. Samples were collected before rough scrub (T0), immediately after sterile scrub (T1), and end of surgery (T2). CFU/mL were determined in duplicate and were log-transformed and averaged. ANOVA models compared the immediate reduction (T0-T1) and sustained reduction (T0-T2) between treatment groups. The immediate and sustained log10CFU/mL reduction between groups was not different (P = 0.46, P = 0.42). Both groups achieved at least a 2-log immediate and sustained reduction. Limitations include small population size, short surgical duration, length of follow-up, and researchers were not blinded to treatment during sampling. This study demonstrates efficacy of IPA-based antisepsis, without the need for CHG, and supports further investigation in clinical surgery as an acceptable method of surgical site preparation.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105257"},"PeriodicalIF":1.3,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A F C Nassar, D P Chiebao, C Del Fava, S Miyashiro, V Castro, R A Ogata, J M Yamamora, C A S Monteiro, E J B Monteiro
{"title":"Histopathological and diagnostic aspects of glanders based on a case series from Brazil.","authors":"A F C Nassar, D P Chiebao, C Del Fava, S Miyashiro, V Castro, R A Ogata, J M Yamamora, C A S Monteiro, E J B Monteiro","doi":"10.1016/j.jevs.2024.105248","DOIUrl":"10.1016/j.jevs.2024.105248","url":null,"abstract":"<p><p>Glanders is a zoonotic disease of equids caused by the bacterium Burkholderia mallei, responsible for considerable economic loss. This study aimed to describe the clinical manifestations, pathological findings, and also bacteriological and molecular methods for agent detection in naturally infected animals (16 adult horses and one fetus) detected by serological survey from three glanders outbreaks. Of the 16 horses, 6 (37.5%) did not show clinical signs. After necropsy,samples of organs, lymph nodes, lesions and secretions were collectedfor histopathology, bacterial isolation, and PCR. The clinical and gross alterations mainly comprised nasal and respiratory forms in the three outbreaks, and less of the cutaneous form. All tested animals were positive by PCR (100%, n=17) on at least one sample. Of 121 samples analyzed 8.2% (10/121) isolated B. mallei and 41.3% (50/121) were polymerase chain reaction (PCR) positive. This work highlights the importance of combining diagnostic techniques, such as histopathology microbiological culture and PCR,to confirm cases and characterize the morbidity of glanders as well as considering seropositive animals without glanders clinical signs as potential carrier animals affecting disease control programmes.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105248"},"PeriodicalIF":1.3,"publicationDate":"2024-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ice application without water drainage supports optimal hoof cooling in adult horses.","authors":"K M Folk, R R White, C B Gleason","doi":"10.1016/j.jevs.2024.105255","DOIUrl":"10.1016/j.jevs.2024.105255","url":null,"abstract":"<p><p>Cryotherapy is often used to reduce inflammation in acute equine laminitis cases. Certain hoof temperatures have been suggested as effective in minimizing the inflammatory process; however, there is limited evidence on which methods are best at achieving these temperatures. Our objective was to determine how different methods of cryotherapy influence the rate and extent of cooling for the equine hoof wall. Four horses received three hoof cooling treatments and a control (CON; no treatment application) in a 4 × 4 Latin square design. Treatments included (1) ice surrounding the hoof in a 5 L fluid bag with water drainage holes (DB), (2) ice surrounding the hoof in an undrained bag (UB), and (3) ice in a commercial wader boot (CW). Hoof wall temperatures were collected via thermal imaging for 12 h. Thermally imaged body temperatures and thermometer-based rectal temperatures, heat index, relative humidity, and ambient temperature were recorded. A treatment × time interaction (P < 0.001) was observed for all hoof temperatures. All treatments differed from CON after 2 h post-application, with the UB treatment resulting in the greatest and most sustained reduction in hoof temperatures over the 12 h period (a change of -23.7 °C ± 1.6). The wader boot showed similar trends to the UB treatment but was poorly tolerated by the horses. Environmental effects differed between hoof and body surface. Our findings indicate that cryotherapy treatments that maintain an ice-water slurry around the hoof result in greater decreases in hoof temperatures 2 h post-treatment compared to drained ice application.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105255"},"PeriodicalIF":1.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M DeBoer, T Rieck, L Johnson, H Redenius, K Martinson
{"title":"Effect of hay nets on subluxations, pain-pressure thresholds, and cervical range of motion in the axial skeleton of adult horses.","authors":"M DeBoer, T Rieck, L Johnson, H Redenius, K Martinson","doi":"10.1016/j.jevs.2024.105256","DOIUrl":"10.1016/j.jevs.2024.105256","url":null,"abstract":"<p><p>Horses have become more intensively managed, including the use of hay nets to increase intake time and reduce hay waste, but their impact on health is not well known. The objectives were to compare changes in subluxations, range of motion, and the pain-pressure threshold in the axial skeleton of horses fed hay with (NET) or without (CON) hay nets. In September 2021, 13 adult horses were blocked by bodyweight (BW) and randomly assigned to the NET or CON treatments for one year. After one year, horses switched treatments and the trial concluded in September 2023. Horses were housed in dry lots with free choice access to round bales with or without hay nets (4.45 cm openings). Chiropractic adjustments occurred in May and August each year to note axial skeleton subluxations. Evaluations occurred in June, July, and August to measure cervical range of motion and mechanical nociceptive thresholds (MNTs) of the jaw, poll, and withers. SAS was used and significance was set at P≤0.05. There was an average 1.6-point increase in cervical subluxations observed in NET horses (P<0.05) while no changes were observed for the TMJ and thoracic or lumbar vertebral regions (P>0.05). Average cervical range of motion was 5 degrees greater in NET horses compared to CON horses for chin to chest and chin to fetlock measurements (P≤0.05). No differences were observed in MNTs (P>0.05). These data suggest that netted round bales may only have minor impacts on the axial skeleton of horses over 12-weeks.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105256"},"PeriodicalIF":1.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J C Samper, J C Hernandez Aviles, L F Ramirez-Agamez, C C Love, C Gonzalez-Marin, P Fleury, P Dini, A De La Fuente, R Foss, F L Campos, P J Ross
{"title":"The use of sex-sorted semen in horses.","authors":"J C Samper, J C Hernandez Aviles, L F Ramirez-Agamez, C C Love, C Gonzalez-Marin, P Fleury, P Dini, A De La Fuente, R Foss, F L Campos, P J Ross","doi":"10.1016/j.jevs.2024.105251","DOIUrl":"10.1016/j.jevs.2024.105251","url":null,"abstract":"<p><p>The application of sex-sorted semen in horses has historically lagged the bovine industry due to differences in the reproductive physiology and grater variability in equine semen quality. Recent advancements, including SuperGen™ sorters and Ultraplus™ semen processing methods developed by STgenetics® (Navasota, Texas), have significantly improved the efficiency of the sex-sorting process and reduced the sperm damage previously reported. To facilitate the broader use sex-sorted semen in the equine industry, it is essential to address challenges such as shipping cooled semen to a central sorting facility, minimizing oxidative stress and DNA fragmentation, and developing effective methods for short-term cooling or long-term cryopreservation of the sex-sorted sperm. In this study, we evaluated the feasibility of shipping cooled (8 °C) equine semen to a central facility for sorting and assessed the quality of sperm post-sorting and preservation. Results from the improved process showed enhancements in sperm motility, morphology, viability and DNA fragmentation following sex-sorting. Although there was a slight, consistent increase in lipid peroxidation of sex-sorted sperm, the quality of sperm over the first 24 h post-sorting was comparable to that of conventional (non-sorted) cool-shipped semen. Fertility rates with fresh semen did not differ between conventional and sex-sorted sperm. When using frozen-thawed sex-sorted sperm for intracytoplasmic sperm injection (ICSI), consistent blastocyst production was achieved, albeit at slightly lower rates than those observed with conventional semen for the same stallions. Stallion-specific variability may account for some of these differences. Overall, sex-sorted semen used for ICSI resulted in a higher number of embryos of the desired sex compared to those obtained using conventional semen.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105251"},"PeriodicalIF":1.3,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K L Kaufman, J K Suagee-Bedore, S E Johnson, K M Ely, S J Ghajar, B M McIntosh
{"title":"Horses with previous episodes of laminitis have altered insulin responses to seasonal oral sugar testing and grazing compared to horses with no known history of laminitis.","authors":"K L Kaufman, J K Suagee-Bedore, S E Johnson, K M Ely, S J Ghajar, B M McIntosh","doi":"10.1016/j.jevs.2024.105254","DOIUrl":"10.1016/j.jevs.2024.105254","url":null,"abstract":"<p><p>Forage is essential for equine health and performance, but intake of elevated pasture nonstructural carbohydrates (NSC) may exacerbate metabolic disorders. This study aimed to investigate the influence of laminitis history on metabolic and morphometric responses in grazing horses. Twelve non-pregnant mares (15 ± 3.4 yrs) were selected based on previous diagnosis of laminitis (PRELAM; n=6) or not (NOLAM; n=6). Horses were maintained on 8.5-ha pasture. Weekly pasture samples were clipped at random for nutrient analysis. Monthly blood samples were collected and analyzed for glucose and insulin concentrations. Body weight (BW), body condition score (BCS), and cresty neck score (CNS) were evaluated monthly. Each month, a modified oral sugar test (OST) was used to assess basal (T0) and 75-minute (T75) insulin dynamics following 0.30 mL/kg BW Karo Light Corn Syrup. Data were analyzed using PROC MIXED with repeated measures (SAS Institute). PRELAM had greater CNS (P < 0.001), BW (P < 0.05), and BCS compared to NOLAM (P < 0.05). Non-fasted glucose was influenced by month (P < 0.001) while non-fasted insulin was affected by group by month (P = 0.004). Fasted insulin concentrations (T0) were affected by month and group (P < 0.001) where PRELAM had greater concentrations. T75 glucose and insulin concentrations were affected by month (P < 0.001 and P = 0.002, respectively) and were higher for PRELAM (P = 0.003). These data suggest horses with greater general and regional adiposity, and previous history of laminitis, often exhibit greater insulin concentrations on pasture and to OST.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105254"},"PeriodicalIF":1.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L Radovic, V Remer, D Rigler, S Felkel, G Brem, B Wallner
{"title":"Y-chromosomal insights into the breeding history and sire line genealogies of two traditional Baroque horse breeds: Lipizzaner and Kladruber.","authors":"L Radovic, V Remer, D Rigler, S Felkel, G Brem, B Wallner","doi":"10.1016/j.jevs.2024.105252","DOIUrl":"10.1016/j.jevs.2024.105252","url":null,"abstract":"<p><p>The paternally inherited, male-specific part of the Y chromosome (MSY) is an ideal marker for studying the origin, genealogies, and historical connections of horse patrilines. Here, we performed fine-scaled MSY haplotype (HT) analysis in two Baroque horse breeds, the Lipizzaner and the Kladruber, both known for their long-standing tradition of sire line breeding and interconnected genealogies. We genotyped 95 MSY markers using KASP<sup>TM</sup> technology in 90 stallions representing all patrilines of both breeds. We identified 14 HTs across eight Lipizzaner sire lines and six HTs in eight Kladruber lines. Y-chromosomal analysis confirmed the presumed Arabian and Iberian origins of two Lipizzaner and two Kladruber foundation sires. Interestingly, six Lipizzaner sire lines clustered into the MSY haplogroup (HG) daC_Tb, a HG previously associated with the Turkoman horse, suggesting a Turkish origin of several Lipizzaner foundation sires, contrary to documented records. We also found evidence for an undocumented Arabian founder in the Lipizzaner breed. Furthermore, three private HTs were detected in Kladruber horses, highlighting the uniqueness of their foundation sires. HTs in shared patrilines between Lipizzaner and Kladruber were consistent, while three Lipizzaner sublines showed discrepancies between MSY results and recorded pedigree data. In conclusion, MSY haplotyping validated historical breed documentation, revealed new insights into the origins and distribution of sire lines, and proved effective in resolving parentage issues across generations.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105252"},"PeriodicalIF":1.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M L A Kneipp, L N Sousa, L O Cota, B D Malacarne, I C Winter, C H Santana, R L Santos, A B S Xavier, R R Faleiros, A M Carvalho
{"title":"Bilateral coxofemoral dysplasia in a Mangalarga Marchador foal.","authors":"M L A Kneipp, L N Sousa, L O Cota, B D Malacarne, I C Winter, C H Santana, R L Santos, A B S Xavier, R R Faleiros, A M Carvalho","doi":"10.1016/j.jevs.2024.105253","DOIUrl":"10.1016/j.jevs.2024.105253","url":null,"abstract":"<p><p>Coxofemoral dysplasia is a condition characterized by the abnormal development and flattening of the femoral head and deformities in the acetabulum, leading to joint instability. Although described in various animal species, it is uncommon and rarely reported in horses. This article presents a case of incapacitating coxofemoral dysplasia diagnosed in a four-month-old foal Mangalarga Marchador, which exhibited lameness in the hind limbs, thoracolumbar kyphosis, gluteal muscle atrophy, and pronounced sensitivity during pelvic limb flexion. The clinical diagnosis indicative of bilateral coxofemoral dysplasia was confirmed through post-mortem examination. This case report highlights the importance of awareness and monitoring for early detection of coxofemoral dysplasia in foals.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105253"},"PeriodicalIF":1.3,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142791582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Veronika Solbach, Markus Grabatin, Yury Zablotski, Robert Fux, Holm Zerbe, Tanja Semira Witte
{"title":"Prevalence of Taylorella equigenitalis in Icelandic mares and geldings in Southern Germany and Austria.","authors":"Veronika Solbach, Markus Grabatin, Yury Zablotski, Robert Fux, Holm Zerbe, Tanja Semira Witte","doi":"10.1016/j.jevs.2024.105247","DOIUrl":"10.1016/j.jevs.2024.105247","url":null,"abstract":"<p><p>Contagious Equine Metritis (CEM) caused by the bacterium Taylorella equigenitalis (T. equigenitalis), is a venereal infection of equids which is of international concern to the equine breeding industry. A recent study showed a high prevalence of T. equigenitalis in Icelandic stallions when compared to stallions of other breeds also using for natural breeding. Consequently, the objectives of the present study were to investigate the prevalence of T. equigenitalis in Icelandic mares and geldings and to determine factors associated with a T. equigenitalis-positive qPCR result. In total, 361 Icelandic horses located in Southern Germany and Austria were tested for T. equigenitalis using a qPCR assay. An overall prevalence of 14.4 % was detected. Positive qPCR results were found in 2.2 % (3/134) of brood mares, 9.0 % (11/122) of maiden mares and in 36.2 % (38/105) of geldings. The odds for a T. equigenitalis-positive qPCR result were significantly lower in both brood (OR = 40.1, 95 % CI: 8.38-192, P < 0.001) and maiden mares (OR = 9.51, 95 % CI: 3.26-25.7, P < 0.001) when compared to geldings. Advancing age was not associated with higher odds for a T. equigenitalis-positive qPCR result (OR = 0.98, 95 % CI: 0.94-1.03, P = 0.51). However, horses of the younger age group showed significantly lower C<sub>t</sub> values compared to horses of the older age group (P = 0.04). Furthermore, geldings showed significantly lower C<sub>t</sub> values than brood (P < 0.03) and maiden mares (P < 0.001). This study showed a significantly higher prevalence of T. equigenitalis in Icelandic geldings compared to Icelandic mares. Icelandic geldings might therefore represent a reservoir for T. equigenitalis.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105247"},"PeriodicalIF":1.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}