{"title":"Seminal vesiculitis and blocked ampullae occurring simultaneously in a stallion","authors":"L. Huggins , P. Dini , E. Santos , G. Dujovne","doi":"10.1016/j.jevs.2025.105629","DOIUrl":"10.1016/j.jevs.2025.105629","url":null,"abstract":"<div><div>A Gypsy Vanner stallion presented with a history of subfertility and abnormal dense, grey ejaculate. Reproductive examination including ultrasound, cytology, and bacteriology confirmed the diagnosis of seminal vesiculitis. After evaluation of several ejaculates, bilateral blocked ampullae were confirmed by azoospermia and a low level of alkaline phosphatase (30 IU/L) in the filtered fraction. Continuous semen collections, along with oral antibiotics treatment, were performed at the owner’s facility for five weeks. After readmission, a successful collection was obtained with a high concentration and low motility. Seminal vesiculitis persisted, local antibiotic treatment of the seminal vesicles (SV) was initiated using video endoscopy, to improve response to treatment. After completing a total of 7 weeks of oral and local SV treatment, the seminal vesiculitis resolved. Despite persistent low semen quality, the stallion has successfully impregnated 2 out of 5 mares (40 %), with confirmed pregnancies and heartbeats.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"151 ","pages":"Article 105629"},"PeriodicalIF":1.3,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144275070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of a thermal camera in measuring surface temperature in horses","authors":"R.M. Ribeiro, T.O. Oliveira, D.S.F. Ribeiro","doi":"10.1016/j.jevs.2025.105614","DOIUrl":"10.1016/j.jevs.2025.105614","url":null,"abstract":"<div><div>The use of thermal cameras in horses has proven effective for monitoring surface body temperature non-invasively, enabling the early identification of disease and inflammation. This study evaluated the agreement between a thermal camera compared to an infrared thermometer in the measurement of surface temperatures of 60 healthy horses (27 mares, 33 stallions; 7.88 ± 4.01 years; 466 ± 50.1 kg). Measurements were taken at the neck, thorax, and coronary band between 8:00–9:00 a.m., under controlled environmental conditions. Analysis revealed strong correlations between the two methods at the coronary band (r = 0.81) and moderate correlations at the neck and thorax (r = 0.53, r = 0.67, respectively). However, the Bland-Altman method indicated wide limits of agreement, particularly in the neck region and a systematic bias, with the thermal camera generally recording higher temperatures. Despite this, the moderate to strong correlations between the thermal camera and infrared thermometer suggest its potential for tracking thermal trends and identifying localized temperature variations, particularly with careful calibration.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"151 ","pages":"Article 105614"},"PeriodicalIF":1.3,"publicationDate":"2025-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144208706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Forni , N. Ellero , A. Mannini , L. Scacco , F. Freccero
{"title":"Headshaking associated with a unique presentation of guttural pouches infection and otitis media in a Quarter Horse stallion: diagnosis, medical treatment and outcome","authors":"G. Forni , N. Ellero , A. Mannini , L. Scacco , F. Freccero","doi":"10.1016/j.jevs.2025.105616","DOIUrl":"10.1016/j.jevs.2025.105616","url":null,"abstract":"<div><div>A 3-year-old Quarter Horse stallion was referred for headshaking (HS) syndrome of one month duration. The horse underwent complete HS workup. Physical examination revealed up-and-down head movements associated with nose snorting during lunging and riding, in the absence of other abnormalities at rest or in motion. Lameness, neurologic and behavioural evaluations were unremarkable. Endoscopic examination of the upper respiratory tract showed bilateral guttural pouches (GPs) inflammation. The mucosal surface was altered by the presence of areas of microbullous appearance coalescing into some bullae of larger diameter. A bacterial culture obtained by flushing, brushing and biopsy of the GP mucosa was positive for <em>Serratia marcescens</em>. Computed tomographic evaluation was indicative of: moderate bilateral GP inflammation, bilateral otitis media, and nuchal ligament enthesopathy, with uncertain significance. The horse was treated with antimicrobials according to the sensitivity test, systemic NSAIDs and rest. At the one-month follow-up, HS condition was improved and the endoscopic examination showed a limited number of bullous lesions remaining. Bacterial culture re-obtained from the GPs lavages was positive for <em>Streptococcus equi zooepidemicus</em>. Based on the residual evidence of GP infection, the horse was re-treated with antimicrobials according to the sensitivity test, systemic NSAIDs and rest. At the two-month follow-up, HS condition was considered resolved based on clinical evaluation, the horse resumed gradually training with satisfactory results. Endoscopy and bacteriology showed resolution of GP infection. At one-year follow-up no relapses were reported.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"151 ","pages":"Article 105616"},"PeriodicalIF":1.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144181655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Luiza Maria Feitosa Ribeiro , João Victor Bersot Gomes , Wilder Hernando Ortiz Vega , Andressa da Silva Alves , Maurício Netto Machado , Luan Junio Wutke , Célia Raquel Quirino , Roberta Carvalho Basile , Paula Alessandra Di Filippo
{"title":"Thermal comfort and seminal traits in stallion reproduction: New methods to understand these relationships","authors":"Luiza Maria Feitosa Ribeiro , João Victor Bersot Gomes , Wilder Hernando Ortiz Vega , Andressa da Silva Alves , Maurício Netto Machado , Luan Junio Wutke , Célia Raquel Quirino , Roberta Carvalho Basile , Paula Alessandra Di Filippo","doi":"10.1016/j.jevs.2025.105615","DOIUrl":"10.1016/j.jevs.2025.105615","url":null,"abstract":"<div><h3>Background</h3><div>Stallion breeding is mostly based on desirable phenotypic traits, with little consideration for semen quality.</div></div><div><h3>Aims/objectives</h3><div>To identify relationships among semen parameters in stallions using a non-invasive, integrative approach across summer and winter seasons.</div></div><div><h3>Methods</h3><div>Twenty-four stallions were evaluated, in which semen collections, testicular thermography, and Doppler velocimetry of the testicular artery by Doppler ultrasonography were performed, in addition to the measurement of physiological, environmental, and heat stress parameters, both in winter (coat weather) and summer (T-shirt weather). Statistical tests included univariate and multivariate analyses in software SPSS® (IBM).</div></div><div><h3>Results</h3><div>Sperm defects (22.4 ± 7.3 %) and heat stress indices (THI: 79.7 ± 3.4; ThStress: 2.9 ± 0.7) were higher in summer (28.6 ± 2.7 °C) than in winter (12.5 ± 9.4 %; 21.1 ± 2.4 °C; THI: 73.5 ± 8.6; ThStress: 1.1 ± 0.3). The heat stress indices were calculated by the equation: THI= [0.8 x (EnTemp + RH/100)] x [(EnTemp – 14.4) + 46.4]. In contrast, systolic/diastolic testicular blood flow velocities, motility, and vigor were greater in winter (33.3 ± 9.3 cm/s; 5.9 ± 2.2 cm/s; 81.2 ± 8.5 %; 3.2 ± 0.4) than in summer (24.4 ± 6.7 cm/s; 4.0 ± 1.0 cm/s; 64.2 ± 21 %; 2.7 ± 0.6). Significant correlations were found between thermal comfort indices and seminal traits in summer, and between Doppler/thermographic data and semen quality in winter.</div></div><div><h3>Conclusion</h3><div>The analyzed indices characterized thermal, circulatory, and seminal parameters in stallions, highlighting seasonal differences and correlations, and supporting the use of Doppler ultrasonography and infrared thermography in fertility assessment, thermal comfort, and optimal breeding season selection.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"151 ","pages":"Article 105615"},"PeriodicalIF":1.3,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144180013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P.V.L.F. Oliveira , L.G.T.M. Segabinazzi , A.M. Crespilho , J.V. Oliveira , A.C. Silva , C.P. Freitas-Dell’Aqua , J.A. Dell’Aqua Jr , F.O. Papa
{"title":"Autologous seminal plasma and post-breeding uterine lavage as alternative options to improve fertility rates of jennies inseminated with cryopreserved jack semen","authors":"P.V.L.F. Oliveira , L.G.T.M. Segabinazzi , A.M. Crespilho , J.V. Oliveira , A.C. Silva , C.P. Freitas-Dell’Aqua , J.A. Dell’Aqua Jr , F.O. Papa","doi":"10.1016/j.jevs.2025.105613","DOIUrl":"10.1016/j.jevs.2025.105613","url":null,"abstract":"<div><h3>Background</h3><div>Poor fertility of cryopreserved semen remains a challenge in donkeys.</div></div><div><h3>Aims/objectives</h3><div>This study evaluated strategies to enhance fertility of cryopreserved semen in donkeys.</div></div><div><h3>Methods</h3><div>Experiment 1 assessed <em>in vitro</em> parameters of semen cryopreserved with two freezing-semen protocols (BC and mINRA). For evaluation, samples were thawed and split into two aliquots: one served as a control, and the second was re-extended with seminal plasma (SP). Sperm motility, plasma membrane and acrosome integrity (PMAI), lipid peroxidation (LPO), and hydrogen peroxide (H₂O₂) were analyzed. Experiment 2 involved 86 estrous cycles of 33 jennies, inseminated with semen cryopreserved using BC (n = 43 cycles) or mINRA (n = 43 cycles). Each group included control cycles (BC, n = 10; mINRA, n = 10) and three treatments: SP, frozen-thawed semen re-extended in SP (BC, n = 08; mINRA, n = 08); UL, uterine lavage (UL) 10 h post-AI (BC, n = 11; mINRA, n = 11); and SP+UL, frozen-thawed semen re-extended in SP followed by UL 10 h post-AI (BC, n = 14; mINRA, n = 14).</div></div><div><h3>Results</h3><div>Post-thaw motility was similar between cryopreservation protocols (P > 0.05), but adding SP reduced the motility parameters of cryopreserved semen (P < 0.05). PMAI was higher, and LPO and H<sub>2</sub>O<sub>2</sub> were lower in semen cryopreserved using BC (P < 0.05). Seminal plasma addition did not affect PMAI but reduced LPO and H<sub>2</sub>O<sub>2</sub> (P < 0.05). Pregnancy rates did not differ between semen cryopreserved using BC (21 %) and mINRA (23 %; P > 0.05). The SP+UL-assigned cycles had higher fertility rates (43 %; P = 0.01) compared to control-assigned cycles (0/20), whereas SP- (13 %) and UL-cycles (23 %) had intermediate pregnancy rates (P > 0.05).</div></div><div><h3>Conclusion</h3><div>Cryopreservation protocol did not impact sperm quality or fertility. However, the association of semen re-extension in SP and post-insemination UL improved fertility outcomes in jennies.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"150 ","pages":"Article 105613"},"PeriodicalIF":1.3,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F.M. Pastor, A.L.S. Miranda, N.C.C. Monteiro, T.S. Carvalho, C.A. Diamantino, S.O. Barros, L.E.C. Torres, F.O. Paes Leme, R.P.A. Maranhão, N.M. Ocarino, R. Serakides
{"title":"Chronic pancreatitis associated with hypertriglyceridemia in a domestic donkey (Equus asinus)","authors":"F.M. Pastor, A.L.S. Miranda, N.C.C. Monteiro, T.S. Carvalho, C.A. Diamantino, S.O. Barros, L.E.C. Torres, F.O. Paes Leme, R.P.A. Maranhão, N.M. Ocarino, R. Serakides","doi":"10.1016/j.jevs.2025.105612","DOIUrl":"10.1016/j.jevs.2025.105612","url":null,"abstract":"<div><div>Pancreatitis is extremely rare in domestic donkeys; hence, definitive antemortem diagnosis is difficult. Herein, we report the case of a four-year-old male domestic Pêga donkey (<em>Equus asinus</em>) with signs of abdominal pain and dysuria. The donkey was fed a high-calorie diet, was obese, and had high serum concentrations of triglycerides, lipase, and amylase. Despite analgesia, abdominal pain was uncontrollable, and the animal was euthanized. Clinical, laboratory, and pathological findings confirmed the diagnosis of chronic, multifocal to coalescent, lymphocytic, and necrotizing pancreatitis associated with hypertriglyceridemia. Pancreatitis should be considered in the differential diagnosis of equids that present with abdominal pain of unknown origin, particularly in those with high serum triglyceride concentrations. Appropriate species management, including a balanced diet and regular exercise, may help prevent obesity and the onset of associated endocrine and metabolic complications.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"150 ","pages":"Article 105612"},"PeriodicalIF":1.3,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Survey of veterinarians’ usage and satisfaction with intra-articular polyacrylamide gel in horses","authors":"Mathilde Pluim , Thibault Frippiat","doi":"10.1016/j.jevs.2025.105610","DOIUrl":"10.1016/j.jevs.2025.105610","url":null,"abstract":"<div><div>Polyacrylamide gel is increasingly used in equine veterinary medicine for osteoarthritis and other joint disorders. This study aimed to document the indications, treatment protocols, rehabilitation plans, outcomes, and satisfaction with intra-articular polyacrylamide gel in horses, as reported by equine veterinarians. An online questionnaire was distributed to practitioners through the European and American Colleges of Veterinary Sports Medicine and Rehabilitation mailing lists, social media, and direct contact. Of 197 respondents, 160 completed the survey, forming the basis for the descriptive statistical analysis. The primary indications for polyacrylamide gel use were chronic synovitis/osteoarthritis (87.1%) and failure of prior joint treatment (83.6%). Treatment protocols, post-treatment rehabilitation plans, and clinical outcomes varied among practitioners and respondent groups based on experience and disciplinary focus (assessed using automated A/B or Kruskal-Wallis with post-hoc Dunn’s test). Most respondents reported return to full exercise from week 4 post-treatment. Complications, worsening, or lack of improvement following treatment were rare, while complete recovery, partial improvement, or transient improvement were commonly observed. Median satisfaction score among respondents was rated 8/10. As expected with the study design, the responses limited detailed insights into individual experiences and may reflect response bias, with most respondents being specialized or focused on equine orthopedics. Despite these limitations, the survey highlights a general good satisfaction with intra-articular polyacrylamide gel, mainly for chronic synovitis/osteoarthritis and use after failure of prior joint treatment, with low reported complication rates and favorable outcomes. These findings support developing standardized guidelines for intra-articular polyacrylamide gel treatment and post-treatment rehabilitation protocols in horses.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"150 ","pages":"Article 105610"},"PeriodicalIF":1.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
R. Rinnovati , A. Spadari , A. Malpighi , F. Meistro , M.V. Ralletti , E. Marcucci , M.A. Tarasconi , R. Lo Chiatto , S. Della Tommasa
{"title":"Evaluation of an intra-articular carboxymethylcellulose crosslinked hydrogel in horses with osteoarthritis","authors":"R. Rinnovati , A. Spadari , A. Malpighi , F. Meistro , M.V. Ralletti , E. Marcucci , M.A. Tarasconi , R. Lo Chiatto , S. Della Tommasa","doi":"10.1016/j.jevs.2025.105611","DOIUrl":"10.1016/j.jevs.2025.105611","url":null,"abstract":"<div><h3>Background</h3><div>Numerous treatment strategies have been proposed to manage osteoarthritis (OA) in sport horses. Carboxymethylcellulose (CMC), is used in veterinary medicine for various purposes.</div></div><div><h3>Aims/objectives</h3><div>This study aimed to assess the efficacy of a CMC hydrogel in reducing clinical lameness in horses with OA affecting joint. The hypothesis was that intra-articular CMC would produce a sustained reduction in lameness compared to a control treatment.</div></div><div><h3>Methods</h3><div>Thirty client-owned horses diagnosed with OA-related lameness in the metacarpophalangeal joint were enrolled. Horses were randomly assigned to two groups: the treated group (n = 16) received a single intra-articular injection of CMC hydrogel (3 ml/joint); the control group (n = 14) received 12 mg/joint of triamcinolone acetonide (TA) and 20 mg/joint of hyaluronic acid (HA). Lameness was evaluated at baseline and at 15, 30, and 90 days using a standardized AAEP scoring system. Treatment success was defined as a reduction of at least one lameness grade without additional therapy. Statistical analysis included the Kruskal–Wallis test and Chi-Square test; significance was set at P < 0.05.</div></div><div><h3>Results</h3><div>At 90 days, 12/16 horses (75%) in the treated group achieved success (mean score 1.3 ± 0.6), compared to 1/14 horses (7.1%) in the control group (mean reduction 0.2 ± 0.4; P = 0.001). At 15 days, 3/15 CMC horses (20%) improved vs. 13/14 controls (90%). At 30 days, success was 75% in both groups.</div></div><div><h3>Conclusion</h3><div>Intra-articular administration of CMC hydrogel provided a delayed but longer-lasting improvement in lameness compared to corticosteroid and HA treatment.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"150 ","pages":"Article 105611"},"PeriodicalIF":1.3,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}