Journal of Equine Veterinary Science最新文献_第2页

Sequencing and assembling the genome of Przewalski's horse in the classroom

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-16 DOI: 10.1016/j.jevs.2025.105383

Christopher Faulk

引用次数: 0

Evaluating plasma lactate running speed derived parameters for predicting maximal lactate steady state in teaching horses

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-15 DOI: 10.1016/j.jevs.2025.105385

T.O. Littiere , G.B. Costa , N.A.A. Sales , J.R.G. Carvalho , I.D.M. Rodriguez , G.V. Ramos , G.C. Ferraz

{"title":"Evaluating plasma lactate running speed derived parameters for predicting maximal lactate steady state in teaching horses","authors":"T.O. Littiere , G.B. Costa , N.A.A. Sales , J.R.G. Carvalho , I.D.M. Rodriguez , G.V. Ramos , G.C. Ferraz","doi":"10.1016/j.jevs.2025.105385","DOIUrl":"10.1016/j.jevs.2025.105385","url":null,"abstract":"<div><div>In humans, there is an ongoing academic discussion about measuring plasma lactate concentration ([La<sup>−</sup>]) during an incremental exercise test (IET) to establish thresholds that can predict the maximal lactate steady state (MLSS). Training studies on horses have utilized the onset of [La<sup>−</sup>] (OBLA<sub>4 mM</sub>), also known as V<sub>4</sub>, the velocity at which the [La<sup>−</sup>] of 4 mM is reached. This study assesses the effectiveness of four methods for estimating the MLSS in teaching horses. Ten teaching horses underwent a single IET to determine four exercise intensity thresholds for predicting MLSS and performed several constant intensity running bouts to obtain the MLSS. The velocity corresponding to the visual lactate threshold (V<sub>LTV</sub>) reached by the horses during IET was the intensity in the first bout. A randomized and blind trial used a visual analysis (LT<sub>V</sub>) and a bi-segmented linear regression model (LT<sub>BI</sub>). The agreement among the velocities corresponding to the V<sub>2</sub>, V<sub>4</sub>, V<sub>LTV</sub>, and V<sub>LTBI</sub> and the MLSS (V<sub>MLSS</sub>) was established using mean difference (MD), ordinary least products (OLP), and correlation coefficient (<em>r</em>). The average plasma [La<sup>−</sup>] at MLSS was 1.50 ± 0.37 mM, and the V<sub>4</sub> was higher than V<sub>MLSS</sub> with an MD of 2.12 ± 0.59 m/s between them. V<sub>2</sub> and V<sub>LTV</sub> showed the lowest mean bias when compared to the V<sub>MLSS</sub>. The V<sub>4</sub> threshold, as determined with the IET protocol used, is unsuitable for estimating MLSS. In the exercise test protocol used in this study, predicting the V<sub>MLSS</sub> based on V<sub>2</sub>, V<sub>LTV</sub>, or V<sub>LTBI</sub> may be more accurate.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"147 ","pages":"Article 105385"},"PeriodicalIF":1.3,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early effects of anti-TNFα antibodies in horses with osteoarthritis

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-14 DOI: 10.1016/j.jevs.2025.105384

G. Perrone , C. Giampaoli , A. Lorenzo Smirnoff , A. Ochoa , R. Pareja , E. De Simone

{"title":"Early effects of anti-TNFα antibodies in horses with osteoarthritis","authors":"G. Perrone , C. Giampaoli , A. Lorenzo Smirnoff , A. Ochoa , R. Pareja , E. De Simone","doi":"10.1016/j.jevs.2025.105384","DOIUrl":"10.1016/j.jevs.2025.105384","url":null,"abstract":"<div><div>Osteoarthritis (OA) is the most common pathology in sport horses, presenting a chronic and recurrent course. Traditional anti-inflammatory treatments provide only temporary clinical improvement and pain relief; however, they have been associated with metabolic disorders in articular cartilage in the medium term. In this study, we generated a llama polyclonal antibody against equine TNF-α, which was used for intra-articular treatment in horses with OA. Treatment effects were assessed through clinical examination and the determination of inflammatory parameters (MMP-2, MMP-9, LDH, and GAGs) in synovial fluid prior to treatment (T0) and on day 30 (T30). Treated animals showed significant improvement in the clinical score, which decreased from 8.47 ± 2.57 to 6.16 ± 1.71 (mean ± SD) on T30 (<em>p</em> < 0.001). Additionally, a reduction in the activity of MMP-2 (188 % ± 82.99 to 147 % ± 40.6 %, <em>p</em> < 0.05), MMP-9 (100 % ± 61.28 to 74.37 % ± 64.26, <em>p</em> < 0.05), and LDH (239.3 IU/l ± 147.4 to 143 IU/l ± 61.21, <em>p</em> < 0.01) was observed. Furthermore, on T30, GAGs levels were significantly increased (1.167 ± 0.46 mg/ml to 1.439 ± 0.267 mg/ml, <em>p</em> < 0.01). These results indicate that the local blockade of pro-inflammatory molecules such as TNF-α reduces pain and the levels of inflammatory molecules associated with catabolic processes and joint damage.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"147 ","pages":"Article 105384"},"PeriodicalIF":1.3,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mild radiographic abnormalities of thoracolumbar spinous processes are not associated with decreased performance in 100 Warmblood jumping horses in active competition

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-11 DOI: 10.1016/j.jevs.2025.105366

M.P. Kerbert , S.S. le Jeune , K.L. Philips , K.L. Shaw , C.A. Erickson , P. Espinosa-Mur

{"title":"Mild radiographic abnormalities of thoracolumbar spinous processes are not associated with decreased performance in 100 Warmblood jumping horses in active competition","authors":"M.P. Kerbert , S.S. le Jeune , K.L. Philips , K.L. Shaw , C.A. Erickson , P. Espinosa-Mur","doi":"10.1016/j.jevs.2025.105366","DOIUrl":"10.1016/j.jevs.2025.105366","url":null,"abstract":"<div><div>Back pain is a common cause of poor performance in horses. However, the relationship between clinical signs and radiographic abnormalities is unclear. The aim of this study was to report radiographic findings of spinous processes in a population of Warmblood jumping horses and their association with performance. This was a descriptive observational study. One hundred Warmblood jumping horses (competing fence height: median 1.35 m, mean 1.3 m, range 0.90m-1.60 m), with no observed lameness and neurological signs, competing in 2018 were selected. Radiographs of spinous processes of the thoracolumbar region were taken and graded for presence of narrowed interspinous space, sclerosis, lucency and enthesopathy (absent, mild, moderate-severe) by four observers. The agreement between the four observers was studied by using the Intraclass Correlation Coefficient. Signalment, current level of performance, highest career level and years of competition were recorded and their correlation with radiographic abnormalities was analyzed.</div><div>There were no horses without radiographic abnormalities in this study. Agreement between observers was moderate-good. Narrowed interspinous spaces and sclerosis were common and mainly found between T15-L1, while enthesopathy was mainly found at the withers (T8-T13). Highest career level was positively correlated with narrowed interspinous spaces (<em>r</em> = .21, <em>p</em> = 0.037) and sclerosis (<em>r</em> = .21, <em>p</em> = 0.034). Current level of performance, age and years of competition did not significantly correlate with presence of radiographic abnormalities (<em>p</em> > 0.05).</div><div>In conclusion, mild radiographic abnormalities of spinous processes of the thoracolumbar spine were common in the current study population of Warmbloods in active jumping competition but did not necessarily negatively affect performance.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"147 ","pages":"Article 105366"},"PeriodicalIF":1.3,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Safety and immunogenicity of a sarcoid vaccine in horses

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-10 DOI: 10.1016/j.jevs.2025.105381

O. Jacob , B. Hause , K. Peters-Smith , E.N. Adam , A.E. Page , C. Floyd , C. Tucker , L.G. Eertink , D. Wang , F. Li

{"title":"Safety and immunogenicity of a sarcoid vaccine in horses","authors":"O. Jacob , B. Hause , K. Peters-Smith , E.N. Adam , A.E. Page , C. Floyd , C. Tucker , L.G. Eertink , D. Wang , F. Li","doi":"10.1016/j.jevs.2025.105381","DOIUrl":"10.1016/j.jevs.2025.105381","url":null,"abstract":"<div><div>Equine sarcoids are the most identified skin tumors of horses, which are highly associated with bovine papillomavirus infection. Sarcoids can impair the use of the horse and are difficult to treat, resulting in significant economic losses and a welfare concern. There is no vaccine available to protect global equines from sarcoids. We aimed to determine the safety and protective antibody response in horses immunized with a recombinant baculovirus vector vaccine expressing the L1 protein of bovine papillomavirus type 1 (BPV1). A group of 10 clinically healthy, sarcoid-free horses were immunized intramuscularly with 1 mL of the equine sarcoid vaccine in the neck twice at 3-week intervals. A control group of 5 clinically normal horses were immunized with culture medium. Clinical signs were monitored daily, while serum samples were collected from all horses before vaccination as well as periodically post-vaccination. All horses appeared normal clinically and no clinically relevant adverse reactions to vaccination were observed. Using a GFP-expressing pseudovirus based virus-neutralizing antibody assay, a robust neutralizing antibody response was detected in all immunized horses following two doses. Pre-vaccination serum from the vaccination group and serum samples from the control group had no detectable virus-neutralizing antibodies. All 10 vaccinated horses developed neutralizing antibody titers 40 to greater than 1280. As neutralizing antibodies are an established correlate of protection for BPV1, we anticipate that this vaccine could protect horses from sarcoids.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"146 ","pages":"Article 105381"},"PeriodicalIF":1.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143402731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Echocardiographic and clinical effects of two sedative doses of Dexmedetomidine in healthy horses

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-09 DOI: 10.1016/j.jevs.2025.105382

Ali Roustaei , Majid Masoudifard , Omid Azari , Hadi Keshipour , Omid Koohestani

{"title":"Echocardiographic and clinical effects of two sedative doses of Dexmedetomidine in healthy horses","authors":"Ali Roustaei , Majid Masoudifard , Omid Azari , Hadi Keshipour , Omid Koohestani","doi":"10.1016/j.jevs.2025.105382","DOIUrl":"10.1016/j.jevs.2025.105382","url":null,"abstract":"<div><div>The effects of dexmedetomidine (DEX), as a sedative drug, on echocardiographic measurements have been investigated in many species but not in horses. Therefore, this study aimed to evaluate the echocardiographic and clinical effects of two sedative doses of DEX in healthy horses. Six female crossbred horses weighted 250-330 kg underwent echocardiography before and after sedation by intravenous administration of a low dose of 3.5 μg/kg (LD) and a high dose of 7 μg/kg (HD) of DEX. A significant increase (p≤ 0.05) was observed in the diameters of the pulmonary artery and the left atrium measured at end-systole after HD treatment, as well as the aortic valve diameter measured at end-systole after LD and HD treatments. Furthermore, left and right ventricular internal diameter measured at end-systole increased significantly after LD and HD treatments. The mean and maximum values of pulmonary velocity and pulmonary pressure gradient were significantly decreased after LD and HD treatments. However, sedation did not affect the remaining cardiac dimensions, the various measures of cardiac function, or the presence and intensity of valvular regurgitation. This study showed that two sedative levels of DEX has an identical effect on most echocardiographic parameters, and like other alpha-2 agonists, DEX has an effect on echocardiographic parameters.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"146 ","pages":"Article 105382"},"PeriodicalIF":1.3,"publicationDate":"2025-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143399287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the role of exercise in the detection of selected respiratory pathogens from nose wipes collected from healthy horses

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-08 DOI: 10.1016/j.jevs.2025.105377

C.A. Wright , K. Lawton , S. Barnum , B.W. Craig , B.R. Farwell , J. Blea , C. McCook , N. Pusterla

{"title":"Investigation of the role of exercise in the detection of selected respiratory pathogens from nose wipes collected from healthy horses","authors":"C.A. Wright , K. Lawton , S. Barnum , B.W. Craig , B.R. Farwell , J. Blea , C. McCook , N. Pusterla","doi":"10.1016/j.jevs.2025.105377","DOIUrl":"10.1016/j.jevs.2025.105377","url":null,"abstract":"<div><div>This study aimed to determine if exercise increases the frequency of selected respiratory viruses/bacteria shedding in non-clinical horses using nose wipes. Nose wipes were taken pre- and post- exercise from 133 healthy horses of various ages and disciplines. Wipes were assayed for the presence of selected common (equine herpesvirus-1 (EHV), EHV-4, S. <em>equi</em> spp <em>equi</em>) and commensal (EHV-2, EHV-5, <em>S. equi</em> spp <em>zooepidemicus</em>) respiratory pathogens. The frequency of detection increased significantly (<em>p</em> < 0.05) in the post-exercise wipes for EHV-1, EHV-2, EHV-5, and <em>S. zooepidemicus</em>. EHV-4 and <em>S. equi</em> were only detected in a small number of horses post-exercise. The absolute quantitation of pathogens was significantly lower post-exercise (<em>p</em> < 0.05) for EHV-2, EHV-5, and <em>S. zooepidemicus</em>. While exercise increased the detection rate of EHV-1, EHV-2, EHV-5 and S<em>. zooepidemicus</em> in nose wipes, absolute quantitation of the respective microbes was lower. The use of nose wipes post-exercise to assess the shedding status of selected respiratory viruses/bacteria in non-clinical horses may be an alternative to the more invasive nasal swabs.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"146 ","pages":"Article 105377"},"PeriodicalIF":1.3,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143377400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suspected chlorfenapyr poisoning in a horse

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-07 DOI: 10.1016/j.jevs.2025.105380

B.P. Simões, F.M. Cerri, R.K. Takahira, A.S. Borges, J.P. Oliveira-Filho, R.M. Amorim

引用次数: 0

Estimation of genetic parameters of body conformation and racing performance traits in Yili horses

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-06 DOI: 10.1016/j.jevs.2025.105378

Chuankun Wang , Yaqi Zeng , Jianwen Wang , Tongliang Wang , Xueyan Li , Zhehong Shen , Jun Meng , Xinkui Yao

{"title":"Estimation of genetic parameters of body conformation and racing performance traits in Yili horses","authors":"Chuankun Wang , Yaqi Zeng , Jianwen Wang , Tongliang Wang , Xueyan Li , Zhehong Shen , Jun Meng , Xinkui Yao","doi":"10.1016/j.jevs.2025.105378","DOIUrl":"10.1016/j.jevs.2025.105378","url":null,"abstract":"<div><div>The Yili horse is a riding-type breed that has been continuously bred independently in China. The genetics of body conformation and racing performance traits need to be comprehensively considered in making breeding decisions. This study is the first to estimate the genetic parameters of 13 body conformation and racing performance traits in Yili horses using a linear model. In total, 9937 body conformation and 2576 race records of 9100 Yili horses obtained from 1993 to 2022 were reviewed. The results showed that heritability estimates of body measurement traits were medium to high, ranging from 0.334 (cannon bone perimeter, CP) to 0.720 (withers height, WH), whereas heritability of the linear scale traits were low to medium, ranging from 0.080 (body length score, BLS) to 0.245 (overall score, OS). The heritability of racing performance traits was low to medium, ranging from 0.054 (racing time, RT) to 0.347 (average speed, AS), but there was a high level of repeatability, ranging from 0.341 (ranking score, RS) to 0.604 (AS). Notably, high genetic correlations were estimated among body measurement (0.588 on average) and racing performance traits (0.924 on average). Overall, body measurement traits and racing performance traits were highly influenced by genetics, had high potential for selection. These findings confirm the possibility of carrying out an evaluation program of Yili horses. We are also continuing to track and evaluate the performance of Yili horses and provide research ideas and methodologies for genetic evaluation of other local horse breeds.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"146 ","pages":"Article 105378"},"PeriodicalIF":1.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of Salmonella spp. in pooled environmental samples from an equine veterinary hospital using a novel point-of-care PCR assay

IF 1.3 3区农林科学

Journal of Equine Veterinary Science Pub Date : 2025-02-05 DOI: 10.1016/j.jevs.2025.105376

Nicola Pusterla , Kaila Lawton , Samantha Barnum , Andrej Vitomirov , Selina Anaya , Pramod Naranatt , Himani Swadia , Eric Mendonsa

{"title":"Detection of Salmonella spp. in pooled environmental samples from an equine veterinary hospital using a novel point-of-care PCR assay","authors":"Nicola Pusterla , Kaila Lawton , Samantha Barnum , Andrej Vitomirov , Selina Anaya , Pramod Naranatt , Himani Swadia , Eric Mendonsa","doi":"10.1016/j.jevs.2025.105376","DOIUrl":"10.1016/j.jevs.2025.105376","url":null,"abstract":"<div><div>The objective of this study was to evaluate a point-of-care (POC) PCR assay for the detection of <em>Salmonella</em> spp. in pooled environmental samples collected at an equine veterinary hospital. A total of 945 environmental samples were collected from high-risk areas, including ICU and isolation stalls, high-traffic areas, treatment rooms, and surgical suites. The environmental samples were collected using drag swabs placed in selenite broth and individually incubated at 35 °C for 20 h. Following the incubation period, 1 mL of up to 10 individual environmental samples were pooled together. Each pool was processed for nucleic acid purification, followed by qPCR analysis for <em>Salmonella</em> spp., as well as direct testing using the POC PCR assay. PCR analyses were performed in a masked fashion, i.e., qPCR and POC PCR assay results remained unknown. Follow-up testing by qPCR and POC PCR for individual environmental samples was performed when a positive pool was detected. A total of 135 pools ranging from 6 to 10 samples per pool were tested. Results showed 100 % agreement between qPCR and POC PCR, with 118 and 17 pools testing PCR-negative and -positive, respectively. Testing of individual environmental samples from the 17 PCR-positive pools identified the same <em>Salmonella</em> spp. positive individual environmental samples by both qPCR and POC PCR. The strategy of pooling environmental samples for the PCR testing of <em>Salmonella</em> spp. has shown promise in monitoring high-risk areas in equine veterinary hospitals. The <em>Salmonella</em> spp. POC PCR assay showed excellent agreement with qPCR, further improving compliance by reducing the turn-around time to 24 h from sample collection to analysis.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"146 ","pages":"Article 105376"},"PeriodicalIF":1.3,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143350092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0