Journal of Equine Veterinary Science最新文献

{"title":"Time-activity budget in horses and ponies: A systematic review and meta-analysis on feeding dynamics and management implications","authors":"M. Lamanna ,&nbsp;G. Buonaiuto ,&nbsp;R. Colleluori ,&nbsp;F. Raspa ,&nbsp;E. Valle ,&nbsp;D. Cavallini","doi":"10.1016/j.jevs.2025.105684","DOIUrl":"10.1016/j.jevs.2025.105684","url":null,"abstract":"<div><h3>Background</h3><div>The time-activity budget is a key indicator of animal welfare. This meta-analysis integrated 14 studies (1979-2020) with 364 horses under wild, natural-living, and stabled conditions to evaluate feeding, resting, standing, and locomotion.</div></div><div><h3>Aim</h3><div>The study aimed to categorize daily behavioural patterns in equines, with emphasis on feeding behaviour.</div></div><div><h3>Methods</h3><div>Fixed and random effects models, forest plots, and ANOVA assessed the influence of management, sociality, sex, age, body size, and feeding type. <em>Results:</em> Feeding was greater in free-ranging (56 %) than stabled horses (38 %; <em>p</em> &lt; 0.0001), in grouped (54 %) vs. isolated (39 %; <em>p</em> = 0.0052), in females (64 %) vs. males (48 %; <em>p</em> = 0.0253), and in grazing (56 %) vs. hay-fed horses (39 %; <em>p</em> = 0.0003). Resting was higher in young, small, grouped, and grazing horses. Standing was commoner in stabled, isolated, adult, larger, and hay-fed animals. Locomotion increased in free-ranging, grouped, and grazing horses.</div></div><div><h3>Conclusion</h3><div>Management promoting foraging, social interaction, and locomotion enhances equine welfare.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105684"},"PeriodicalIF":1.6,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145006247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic composition of gastric reflux and small intestinal fluid in horses: implications for fluid replacement","authors":"G.C. Paganelli,&nbsp;H.C. Schott II","doi":"10.1016/j.jevs.2025.105683","DOIUrl":"10.1016/j.jevs.2025.105683","url":null,"abstract":"<div><h3>Background</h3><div>Small intestinal (SI) distension and gastric reflux (GR) are common in horses with mechanical and functional ileus. Removal of GR results in fluid/ion losses.</div></div><div><h3>Aims/objectives</h3><div>1) Determine the capacity of healthy SI; 2) measure ion concentrations in normal SI fluid, GR, or fluid from SI undergoing resection. The authors hypothesized that Na⁺ and Cl^- concentrations would be lower and higher, respectively, in GR as compared to SI fluid from healthy horses, while there would be little difference in K⁺ concentration.</div></div><div><h3>Methods</h3><div>Observational study: two to 15 meters of SI from 15 horses were distended with water to 6-7 cm in diameter. Volume drained was measured to determine capacity. Ion concentrations were measured in SI fluid from healthy horses (<em>n</em>=10), GR (<em>n</em>=11), and strangulated bowel (<em>n</em>=2).</div></div><div><h3>Results</h3><div>Normal SI had a fluid capacity of 2.8 ± 0.6 L/m. Na⁺, K⁺, and Cl^- concentrations were 100 ± 6, 22 ± 5, and 66 ± 30 mmol/L, respectively, in normal SI fluid; 77 ± 16, 16 ± 11, and 96 ± 25 mmol/L, respectively, in GR; and 116 ± 11, 12 ± 5, and 43 ± 27 mmol/L, respectively, in fluid from strangulated SI. Concentrations of Na⁺ were lower (<em>P</em>&lt;0.01) and Cl^- greater (<em>P</em>&lt;0.03) in GR than in normal SI fluid or fluid from strangulated SI, while all fluid K⁺ concentrations were 3-5-fold greater than serum K⁺ concentration.</div></div><div><h3>Conclusion</h3><div>These data can be used to estimate water and ion losses that occur with SI disorders to implement appropriate fluid therapy plans.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105683"},"PeriodicalIF":1.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stable microclimates and autonomic modulation in horses housed in different stable architectures during monsoon in a tropical environment","authors":"Chanoknun Poochipakorn ,&nbsp;Kanokpan Sanigavatee ,&nbsp;Kansuda Leelehapongsathon ,&nbsp;Thita Wonghanchao ,&nbsp;Metha Chanda","doi":"10.1016/j.jevs.2025.105685","DOIUrl":"10.1016/j.jevs.2025.105685","url":null,"abstract":"<div><h3>Background</h3><div>The impact of climate conditions and stable design on horses housed in individual stalls plays a significant role in their well-being, especially in tropical climates. Limited information exists regarding their conditions during the monsoon season.</div></div><div><h3>Objective</h3><div>This study focused on the stable microclimate and autonomic regulation of horses kept in different stable architectures during the monsoon in a tropical setting.</div></div><div><h3>Methods</h3><div>Twenty-two horses were assigned to one of three stable designs, each offering unique microclimates, including relative humidity, air temperature, and various levels of noxious gases. Autonomic modulation was assessed by observing multiple heart rate variability (HRV) metrics at one-hour intervals for 24 consecutive hours.</div></div><div><h3>Results</h3><div>The findings revealed significant variations in stable microclimates across the different designs, with notable differences between day and night. Horses housed in stables with solid external walls and a lower volume-to-horse ratio exhibited lower HRV (<em>p</em> &lt; 0.05-0.0001), indicating more significant stress, compared to those in stables with solid walls and a higher volume-to-horse ratio or those without solid external walls. Furthermore, the study established a correlation between stable microclimates and HRV modulation based on stable design.</div></div><div><h3>Conclusion</h3><div>These results indicate that autonomic responses vary among horses in different stable designs, suggesting that stable architecture, microclimates, and the volume-to-horse ratio significantly influence horses’ stress responses. Overall, these findings offer important insights for improving housing management to enhance the welfare of horses during the monsoon season in tropical environments.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105685"},"PeriodicalIF":1.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo effects of Acacia mearnsii De Wild extract for cyathostomin control in horses","authors":"G.L.S. Silva ,&nbsp;U.A. Curcio ,&nbsp;J.A. Boufleur ,&nbsp;D.G.L. Borges ,&nbsp;M.T.M. Nakatani ,&nbsp;M.G. Freitas ,&nbsp;F.A. Borges ,&nbsp;F.J.C. Faria ,&nbsp;R.H.P. Silva ,&nbsp;G.L. Franco","doi":"10.1016/j.jevs.2025.105686","DOIUrl":"10.1016/j.jevs.2025.105686","url":null,"abstract":"<div><h3>Introduction</h3><div>Anthelmintic resistance has led to the use of organic extracts as alternative methods of parasite control.</div></div><div><h3>Objectives</h3><div>The study aimed to assess the effects of <em>Acacia mearnsii</em> extract (tannin) on the control of cyathostomins in horses, both <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Materials and methods</h3><div>Thirty Pantaneiro horses naturally infected with cyathostomins were sourced from two distinct farms, designated as Farms A and B. At the start of the study, all third-stage larvae were cyathostomins, and an egg hatchability test (EHT) was performed using fecal samples from horses on both farms. Two randomized 28-day field studies were conducted for the fecal egg count reduction test (FECRT) with evaluations on days 0, 7, 14, 21, and 28. On days 0 and 28, body weight (BW), body condition score (BCS), and blood samples for hematological and biochemical analysis were also collected. The horses diet included panicum grass pasture, 1 kg of concentrate, 40 g of liquid sugarcane molasses (Control), and 25 g of <em>A. mearnsii</em> extract.</div></div><div><h3>Results</h3><div>The EC50 values were 13.2 mg/mL (95 % confidence interval [CI]: 11.8–14.7 mg/mL) and 14.9 mg/mL (95 % CI: 13.3–16.9 mg/mL) for Farms A and B, respectively. The FECRT showed no effect (<em>P</em> &gt; 0.05) between the control and treated groups, with mean values of 3.09% and 3.18%, respectively. Blood and hematological parameters also showed no differences (<em>P</em> &gt; 0.05) between treatments.</div></div><div><h3>Conclusion</h3><div>The tannin extract reduced egg hatchability in vitro, but did not lower fecal egg counts in naturally infected horses during the in vivo trial.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105686"},"PeriodicalIF":1.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical evaluation of the ADVIA Centaur XPT chemiluminescent immunoassay for equine insulin measurement.","authors":"R Rey-Conejo, R E Toribio, S Möller, E Müller, P Fores-Jackson","doi":"10.1016/j.jevs.2025.105661","DOIUrl":"10.1016/j.jevs.2025.105661","url":null,"abstract":"<p><strong>Background: </strong>Insulin dysregulation is a key component of equine metabolic syndrome and is commonly assessed using basal insulin concentrations. Different analyzers may yield variable insulin results, limiting comparability.</p><p><strong>Aims/objectives: </strong>To assess the analytical performance of the ADVIA Centaur XPT chemiluminescent immunoassay (CLIA) for equine insulin measurement and to establish adapted decision thresholds based on comparison with a previously used CLIA method.</p><p><strong>Methods: </strong>Precision, linearity, and dilution recovery were assessed for the ADVIA Centaur XPT. A total of 89 equine serum samples submitted for basal insulin testing were measured using the ADVIA Centaur XPT and Immulite 2000 XPi analyzers. Results were compared using Deming regression, Bland-Altman analysis, and weighted kappa statistics to evaluate agreement in classification.</p><p><strong>Results: </strong>Intra-assay coefficients of variation were 3.56 %, 2.01 %, and 1.92 % for low, medium, and high insulin concentrations, respectively; inter-assay variation was 5.19 %, 5.78 %, and 5.68 %. Deming regression showed a proportional bias, with the ADVIA Centaur XPT consistently measuring lower insulin concentrations compared to the Immulite 2000 XPi. Bland-Altman analysis confirmed this bias across the measurement range. Spearman correlation between the two methods was 0.92 (95 % CI: 0.87-0.94; P < 0.0001), indicating a strong association in rank order. Classification agreement using adapted decision limits yielded a weighted kappa of 0.82, indicating strong agreement.</p><p><strong>Conclusion: </strong>The ADVIA Centaur XPT demonstrated acceptable precision. When insulin concentrations were classified using adapted thresholds, the agreement between the two CLIA analyzers was strong, suggesting that the ADVIA Centaur XPT may be suitable for measurement of equine insulin concentration.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105661"},"PeriodicalIF":1.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144768723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation rate and spectrum of germline de novo mutations in a closed population of Thoroughbred horses","authors":"Risako Furukawa ,&nbsp;Teruaki Tozaki ,&nbsp;Keijiro Mizukami ,&nbsp;Yusuke Iwasaki ,&nbsp;Koki Kawate ,&nbsp;Mio Kikuchi ,&nbsp;Taichiro Ishige ,&nbsp;Yukihide Momozawa ,&nbsp;Emiko Fukui ,&nbsp;Hironaga Kakoi","doi":"10.1016/j.jevs.2025.105682","DOIUrl":"10.1016/j.jevs.2025.105682","url":null,"abstract":"<div><h3>Background</h3><div>Thoroughbreds have been maintained as a closed breed for over 300 years since the crossbreeding between Arabian stallions and English mares. Despite interest in germline <em>de novo</em> mutations across species, their frequency in horses, particularly in Thoroughbreds, remains largely unexplored.</div></div><div><h3>Aims/objective</h3><div>This study aimed to identify <em>de novo</em> mutations in Thoroughbreds and estimate their frequency within a genetically closed population.</div></div><div><h3>Methods</h3><div>We performed deep whole-genome sequencing (≥230× depth, 150 bp paired-end reads) and Sanger validation in a Thoroughbred trio (sire, dam, and foal). Reads were aligned to the EquCab3.0 reference genome, and variants in regions with sequencing depth ≥20 (covering 99.6 % of the genome) were analyzed for Mendelian inconsistencies.</div></div><div><h3>Results</h3><div>A total of 48 <em>de novo</em> mutations were identified, comprising 46 single nucleotide substitutions and 2 deletions, none of which were found in public variant databases. Of these, 18 were of paternal origin and 6 were of maternal origin (<em>P</em> &lt; 0.05). The estimated mutation rate was 9.59 × 10⁻⁹, which is in a comparable range to that reported in humans. One mutation (chr7:g.2084761G&gt;<em>A</em>) introduced a nonsense variant in the zinc finger protein 77 gene, which encodes a putative transcriptional regulator.</div></div><div><h3>Conclusions</h3><div><em>De novo</em> mutations were identified in the Thoroughbred genome, and the mutation rate is consistent with estimates from other mammalian studies. A significant bias toward paternal origin was observed. Our findings suggest that germline <em>de novo</em> mutations are a source of novel genetic variation in Thoroughbreds, even within a genetically closed population.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105682"},"PeriodicalIF":1.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How prior racing performance influences competition level in off-the-track Thoroughbred horses’ post-racing eventing careers","authors":"Elouise K Bacon,&nbsp;Danica Parnell,&nbsp;Gary Muscatello,&nbsp;Jaimie McElroy,&nbsp;Brandon D Velie","doi":"10.1016/j.jevs.2025.105679","DOIUrl":"10.1016/j.jevs.2025.105679","url":null,"abstract":"<div><h3>Background</h3><div>The ongoing discussion surrounding the well-being of retired racehorses underscores the importance of implementing effective rehoming strategies that prioritise animal welfare. A significant knowledge gap persists regarding whether a horse’s success in racing can be used to reliably predict its performance in different equestrian sports upon retirement from racing.</div></div><div><h3>Aims/Objectives</h3><div>To examine the association between racehorse performance metrics and eventing metrics in off-the-track Thoroughbreds that compete in eventing within Australia.</div></div><div><h3>Methods</h3><div>Racing metrics and eventing metrics for a sample of off-the-track Thoroughbreds (<em>n</em> = 6,828) registered with Equestrian Australia were collated. Summary statistics of racing metrics were estimated and assessed for differences between horses competing in eventing at level EvA95 and above versus horses competing at levels below EvA95. Generalised linear models were applied to assess the influence of racing performance metrics on eventing performance measures at levels EvA95 and above.</div></div><div><h3>Results</h3><div>Horses that failed to reach an eventing level of EvA95 were shown to have had significantly (<em>P</em> &lt; 0.01) better racing performance and longer racing careers. Time elapsed since last race was shown to be favourably associated with most eventing parameters assessed and longer best race distances were shown to be significantly associated with higher levels of eventing achieved.</div></div><div><h3>Conclusions</h3><div>Riders seeking high-level eventing prospects may want to prioritize Thoroughbreds that excelled at longer race distances; however, racing performance alone does not appear to be a reliable predictor of success in eventing. Other factors, such as stride characteristics and the suitability of the rider-horse partnership, also warrant thoughtful consideration.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105679"},"PeriodicalIF":1.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of mixed polyphenol supplementation on lower airway inflammation in horses with Equine Asthma Syndrome","authors":"K.M. Andrews,&nbsp;L.J. Berghaus,&nbsp;K.A. Hart","doi":"10.1016/j.jevs.2025.105681","DOIUrl":"10.1016/j.jevs.2025.105681","url":null,"abstract":"<div><h3>Background</h3><div>Equine Asthma Syndrome (EAS) is a chronic inflammatory respiratory disease of adult horses. While dietary polyphenols have shown anti-inflammatory effects in human asthma, their use in equine asthma has not been studied.</div></div><div><h3>Aims</h3><div>To evaluate the effects of a mixed polyphenol supplement on systemic and pulmonary inflammation, bronchoalveolar lavage (BAL) cytology, and clinical respiratory signs in healthy and asthmatic horses. We hypothesized that polyphenol supplementation would decrease systemic and pulmonary inflammation in both healthy and asthmatic horses and would improve BAL cytology and clinical respiratory signs in asthmatic horses.</div></div><div><h3>Methods</h3><div>In Part One, 18 horses (eight healthy, 10 asthmatic) received a daily mixed polyphenol supplement for 6–8 weeks. Physical examination, BAL cytology, and cytokine concentrations in serum and BAL fluid were assessed before and after supplementation. In Part Two, 10 asthmatic horses were randomized into supplemented and control groups (<em>n</em> = 5/group). After 4 weeks on a low-dust diet with or without supplementation, airway inflammation was exacerbated using dusty hay for 4 weeks. Clinical respiratory signs, rebreathing exam findings, and pulmonary inflammation were assessed.</div></div><div><h3>Results</h3><div>In asthmatic horses in Part One, BAL % neutrophils and BAL IL-6 concentrations significantly decreased post-supplementation (<em>P</em> = 0.02; 95 % CI = 93.86 to 913.2 pg/ml), and a greater decrease in BAL IL-10 was observed in asthmatic compared to healthy horses (<em>P</em> = 0.02; 95 % CI = -22.4 to -0.01). In Part Two, the proportion of horses with abnormal rebreathing exams differed significantly between groups (<em>P</em> = 0.002; 95 % CI = 0.11 to 0.41), and supplemented horses had significantly lower respiratory rates (<em>P</em> = 0.008; 95 % CI = -18.0 to -4.0).</div></div><div><h3>Conclusions</h3><div>Polyphenol supplementation may modulate pulmonary inflammation and improve clinical signs in mildly asthmatic horses when combined with environmental management.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105681"},"PeriodicalIF":1.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fistulous withers causing spinal epidural abscess in a Thoroughbred mare","authors":"Serena Ceriotti ,&nbsp;Stuart Clark-Price ,&nbsp;Robert Cole ,&nbsp;Alana Kramer ,&nbsp;Maninder Sandey ,&nbsp;Mariano Mora","doi":"10.1016/j.jevs.2025.105680","DOIUrl":"10.1016/j.jevs.2025.105680","url":null,"abstract":"<div><div>This case describes a 15-year-old Thoroughbred mare with fistulous withers that progressed to vertebral osteomyelitis and spinal epidural abscess (SEA), a severe complication that has yet to be reported. Initially treated with antibiotics and anti-inflammatory medication, the mare developed acute hindlimb ataxia and spastic paresis, suggesting spinal cord compression at T3-L3. Diagnostic imaging revealed vertebral fractures and abscessation, but vertebral canal involvement could not be confirmed. Despite aggressive medical therapy, including antimicrobials, corticosteroids, and neuroprotective agents, the mare’s neurological condition deteriorated, leading to euthanasia. Post-mortem findings confirmed an extensive paraspinal abscess that had extended into the epidural space, causing severe spinal cord compression. The isolated pathogen, <em>Escherichia coli</em>, is commonly associated with chronic fistulous withers. This case underscores the importance of considering SEA as a diagnostic differential in horses with chronic paraspinal infections that develop acute neurological decline. Due to diagnostic and treatment limitations in equine medicine, prognosis remains guarded, with early surgical intervention and targeted antimicrobial therapy likely being crucial for better outcomes.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105680"},"PeriodicalIF":1.6,"publicationDate":"2025-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytoplasmic and nuclear Survivin expression in healthy gastric mucosae of equids: A comparative study between horses, donkeys, and mules","authors":"A.L. Medina-Bolívar ,&nbsp;J.D. Muñoz-Duque ,&nbsp;J.R. Martínez-Aranzales","doi":"10.1016/j.jevs.2025.105678","DOIUrl":"10.1016/j.jevs.2025.105678","url":null,"abstract":"<div><h3>Background</h3><div>Information regarding Survivin protein expression in the gastric mucosa of equids is scarce. This protein has been associated with functions related to modulating apoptosis and promoting mitosis in epithelial cells and is considered part of gastric cytoprotection mechanisms in humans and mice, maintaining mucosal integrity and regulating cellular renewal.</div></div><div><h3>Aims/objectives</h3><div>This study aimed to determine the expression of cytoplasmic and nuclear Survivin in gastric mucosae of equids (horses, donkeys, and mules).</div></div><div><h3>Methods</h3><div>A total of 30 healthy stomachs, 10 from each species, were evaluated using histology and immunohistochemistry techniques. Survivin protein was detected with antibody EP119 and with the polymer method.</div></div><div><h3>Results</h3><div>Immunohistochemistry revealed survivin expression in the gastric mucosa of equids. In horses and mules, the survivin index was evident in the cytoplasm of both glandular (59 ± 55; 55 ± 40) and squamous (15 ± 16; 18 ± 19) mucosa, being significantly higher in the glandular mucosa (P = 0.001; P = 0.041), respectively. In contrast, donkeys showed a higher survivin index in the cytoplasm, but no significant difference was observed between the two types of mucosa (P = 0.506).</div></div><div><h3>Conclusion</h3><div>Survivin expression in the healthy gastric tissue of equids was localized to the lamina propria and the basal layer of the epithelial layer, predominantly within the cytoplasmic compartment of the cells. However, further techniques are needed to study this protein in Equine Gastric Ulcer Syndrome (EGUS) to assess its potential role in cytoprotection and gastro-adaptation in these species.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"153 ","pages":"Article 105678"},"PeriodicalIF":1.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}