Veronika Solbach, Markus Grabatin, Yury Zablotski, Robert Fux, Holm Zerbe, Tanja Semira Witte
{"title":"Prevalence of Taylorella equigenitalis in Icelandic mares and geldings in Southern Germany and Austria.","authors":"Veronika Solbach, Markus Grabatin, Yury Zablotski, Robert Fux, Holm Zerbe, Tanja Semira Witte","doi":"10.1016/j.jevs.2024.105247","DOIUrl":"10.1016/j.jevs.2024.105247","url":null,"abstract":"<p><p>Contagious Equine Metritis (CEM) caused by the bacterium Taylorella equigenitalis (T. equigenitalis), is a venereal infection of equids which is of international concern to the equine breeding industry. A recent study showed a high prevalence of T. equigenitalis in Icelandic stallions when compared to stallions of other breeds also using for natural breeding. Consequently, the objectives of the present study were to investigate the prevalence of T. equigenitalis in Icelandic mares and geldings and to determine factors associated with a T. equigenitalis-positive qPCR result. In total, 361 Icelandic horses located in Southern Germany and Austria were tested for T. equigenitalis using a qPCR assay. An overall prevalence of 14.4 % was detected. Positive qPCR results were found in 2.2 % (3/134) of brood mares, 9.0 % (11/122) of maiden mares and in 36.2 % (38/105) of geldings. The odds for a T. equigenitalis-positive qPCR result were significantly lower in both brood (OR = 40.1, 95 % CI: 8.38-192, P < 0.001) and maiden mares (OR = 9.51, 95 % CI: 3.26-25.7, P < 0.001) when compared to geldings. Advancing age was not associated with higher odds for a T. equigenitalis-positive qPCR result (OR = 0.98, 95 % CI: 0.94-1.03, P = 0.51). However, horses of the younger age group showed significantly lower C<sub>t</sub> values compared to horses of the older age group (P = 0.04). Furthermore, geldings showed significantly lower C<sub>t</sub> values than brood (P < 0.03) and maiden mares (P < 0.001). This study showed a significantly higher prevalence of T. equigenitalis in Icelandic geldings compared to Icelandic mares. Icelandic geldings might therefore represent a reservoir for T. equigenitalis.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105247"},"PeriodicalIF":1.3,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Evaluation of a non-radioactive rapid test for the determination of serum progesterone in the mare.","authors":"L Längerer, G Schuler, K Büttner, A Wehrend","doi":"10.1016/j.jevs.2024.105250","DOIUrl":"10.1016/j.jevs.2024.105250","url":null,"abstract":"<p><p>The determination of progesterone from mares' serum plays a decisive role in diagnosing estrus cycle disorders or luteal insufficiency. To date, no measurement methods are available for rapid quantitative diagnosis of serum progesterone in the mare that would allow results to be available within a two-hour time frame. The present study will evaluate a commercial enzyme-linked fluorescent assay, the mini VIDAS device (bioMérieux, Nürtingen, Germany). Serum was prepared from the blood samples of one hundred and seven mares, divided into two aliquots, and stored at -20°C. Subsequently, comparative progesterone measurements were carried out using radioimmunoassay (RIA) and mini VIDAS, with RIA as the reference standard. The results show a strong positive correlation between the two measurement methods (Spearman rank correlation rs = 0.96381 (p < 0.05). The tests confirm that the mini VIDAS can be used for equine serum progesterone concentration determination and provides reliable results in less than an hour. For accurate estrus cycle diagnostics, high precision in the low measurement range is essential, as even minor deviations can result in ovulation misdiagnosis. Despite the high correlation of the measuring methods, deviations from the reference method can be observed in the low measuring range of the mini VIDAS, which suggests potential limitations of this study.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105250"},"PeriodicalIF":1.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Laura Sala-Ayala, Juan Cuervo-Arango, Rebeca Martinez-Bovi
{"title":"Improved embryo recovery through increased flushing media volume and the impact on unintended pregnancies in a single subject donor mare.","authors":"Laura Sala-Ayala, Juan Cuervo-Arango, Rebeca Martinez-Bovi","doi":"10.1016/j.jevs.2024.105246","DOIUrl":"10.1016/j.jevs.2024.105246","url":null,"abstract":"<p><p>The case report discusses the implications of using different volumes of flushing media during embryo flushing (EF) procedures in a single donor mare that experienced multiple unintended pregnancies following negative EF outcomes. The study involved two sets of EF experiments over two years on a non-lactating Spanish purebred mare. The initial experiments (group 1, n = 6) used 1 L of flushing medium (Ringer´s Lactate), and subsequent tests (group 2, n = 5) increased the volume to 2 L, to assess whether a larger volume could enhance embryo recovery rates. After every EF procedure, no PGF2α was administered. The overall frequency of double OV was 36.3 %, and did not vary (p > 0.05) between group 1 and 2 (33.3 % and 40 %). The results indicated a significant improvement (p < 0.05) in embryo recovery rates with the increased medium volume (group 1, 25 %; group 2, 85.7 %). A tendency was noted (p = 0.06) for more positive EF outcomes in group 2 (100 % positive outcome). The number of unintended pregnancies observed was significant (p < 0.05) between groups (group 1, 4/8; group 2, 0/7). The study concluded that a higher volume of flushing medium yields a higher embryo recovery rate and minimizes unintended pregnancies in the subject mare.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105246"},"PeriodicalIF":1.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A Charles, H Vande Kerckhove, L De Maré, D Cassart, J Ficheroulle, G de la Rebière de Pouyade, I Tosi
{"title":"Generalized sarcoidosis associated with hypertrophic osteopathy in a Standardbred racehorse.","authors":"A Charles, H Vande Kerckhove, L De Maré, D Cassart, J Ficheroulle, G de la Rebière de Pouyade, I Tosi","doi":"10.1016/j.jevs.2024.105249","DOIUrl":"10.1016/j.jevs.2024.105249","url":null,"abstract":"<p><p>A Standardbred racehorse was presented for exercise intolerance, weight loss, pyrexia and facial deformity. Radiography and ultrasonography revealed periostitis and regional soft tissue swelling of maxillary bones. Computed tomography excluded any dental or sinus origin of these abnormalities. Further deformities on distal limbs and skin lesions appeared during hospitalization. Radiography identified bilateral periostitis and soft tissue swelling in the distal radius and metatarsal bones, as observed in the head, suggestive of hypertrophic osteopathy (HO). Skin biopsies revealed granulomatous dermatitis, indicating equine sarcoidosis (ES). The horse was euthanized due to rapid deterioration of his condition. Post-mortem examination and histopathology revealed splenomegaly, several pulmonary nodules and disseminated granulomatous lesions. These findings confirmed the diagnosis of generalized ES, or equine idiopathic systemic granulomatous disease. The concurrent with HO was probably associated with the intrathoracic pathology. Polymerase chain reaction (PCR) on pooled tissues identified equine herpesvirus 5 (EHV-5) DNA.</p>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":" ","pages":"105249"},"PeriodicalIF":1.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A.E. de Lima , R.P. Mendes , D.D.V. Piñeros , B.B. Alibrando , A.L.V. de Zoppa , L.C.L.C. da Silva , S.H. de Freitas , R.R. Corrêa
{"title":"Fenestration of the maxillary septal bulla using different surgical approaches and endoscopy systems in horses: An ex vivo study","authors":"A.E. de Lima , R.P. Mendes , D.D.V. Piñeros , B.B. Alibrando , A.L.V. de Zoppa , L.C.L.C. da Silva , S.H. de Freitas , R.R. Corrêa","doi":"10.1016/j.jevs.2024.105229","DOIUrl":"10.1016/j.jevs.2024.105229","url":null,"abstract":"<div><div>Sinusitis is common in horses. Sinoscopy is used for visualization of sinus cavities and can be used for diagnostic and therapeutic purposes. In this surgical procedure, a larger chamber is created and communication with other sinus compartments facilitated. To compare the applicability of different surgical approaches and rigid endoscopy systems for sinus inspection and fenestration of the maxillary septal bulla, and to examine the impact of different lens angles on the effectiveness of the procedure. Six chemically preserved cadaveric equine heads were used. Frontal and caudal maxillary portals were tested. Portals were created in the right and left antimeres of each specimen, over the frontal and the caudal maxillary sinus. Two types of rigid 4 mm endoscopes (0º and 30º) were used. The comprehensiveness of sinus examination and the applicability of portals for fenestration of the maxillary septal bulla were evaluated. Satisfactory exploration of sinus cavities and structures of interest was achieved in all specimens. Fenestration of the maxillary septal bulla was successfully accomplished using frontal portals as viewing portals and caudal maxillary portals as instrument portals. When caudal maxillary portals were used as viewing portals, endoscope maneuverability was limited by the infraorbital canal in 50% of antimeres. When frontal portals were used as instrument portals, the frontomaxillary opening interfered with or prevented appropriate fenestration of the bulla in 83.33 % and 16.66 % of antimeres respectively. Ideally, frontal portals should be used as viewing portals and caudal maxillary portals as instrument portals for successful fenestration of the maxillary septal bulla.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105229"},"PeriodicalIF":1.3,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142715863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jillian E. Guertin , Joao de Agostini Losano , Sophia Salazar, Justin Callaham, Bradford W. Daigneault
{"title":"Prolonged maintenance of stallion semen by optimization of cooling conditions","authors":"Jillian E. Guertin , Joao de Agostini Losano , Sophia Salazar, Justin Callaham, Bradford W. Daigneault","doi":"10.1016/j.jevs.2024.105243","DOIUrl":"10.1016/j.jevs.2024.105243","url":null,"abstract":"<div><div>Bottlenecks to the success of equine assisted reproductive technologies (ART) include suboptimal conditions for prolonged storage of stallion sperm. Shipped stallion sperm are transported in cooling devices designed to maintain temperature for up to 48 h. Increasing the storage time of cooled semen while maintaining acceptable motility would relieve logistical ART challenges. Experiments were conducted to test the hypothesis that external regulation of shipment container temperature would prolong storage time of cooled stallion semen. Initial experiments determined the effect of pre-freezing cooling cans at -20 °C or -80 °C on sperm motility. Fresh sperm was extended in INRA96 and placed in commercial Equitainers for 3.5 days (84 h). Quantification of sperm kinematics was determined every 12 h. Sperm held in Equitainers with -20°C cans maintained higher total and progressive motility than -80 °C conditions at 60 h (63 %, 29 % vs. 32 %, 17 %, respectively). Internal monitoring of Equitainers containing -20 °C freezer can temperature identified 20 °C as the threshold for rapidly decreased motility. In the second experiment, sperm were maintained in Equitainers containing -20 °C freezer cans and placed in two different conditions: 1) ambient temperature for 48 h (23 °C) or 2) 5 °C for external temperature regulation up to 8 days, or when total motility dropped below 50 %. Sperm kinematics was determined every 24 h. Total motility from samples held with external temperature regulation (5 °C) remained above 50 % for more than 7 days. These findings suggest that cooled stallion semen can be advanced beyond traditional 48 h constraints by improving temperature maintenance of storage conditions.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105243"},"PeriodicalIF":1.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Denyus Augusto de Oliveira Padilha , Suelen Fernandes Padilha , Rafaela Martins , Bruna Elisa Machado Scheffer , Mayumi Regina Miliorini , Laila Talarico Dias , Rodrigo de Almeida Teixeira
{"title":"Estimation of genetic parameters for racing time and ranking in Thoroughbred horses","authors":"Denyus Augusto de Oliveira Padilha , Suelen Fernandes Padilha , Rafaela Martins , Bruna Elisa Machado Scheffer , Mayumi Regina Miliorini , Laila Talarico Dias , Rodrigo de Almeida Teixeira","doi":"10.1016/j.jevs.2024.105244","DOIUrl":"10.1016/j.jevs.2024.105244","url":null,"abstract":"<div><div>This study aimed to estimate genetic parameters for race time in seconds and final ranking, as well as to analyze the genetic trends associated with race time. The study utilized a dataset consisting of 23,290 records of race times and final ranks at distances of 1,000, 1,600, and 2,000 m from 6,213 Thoroughbred horses from the São Paulo Jockey Club. Our model considered the year of the run, animal sex, race class, track conditions, the linear effect of horse weight and age, and the quadratic effect of age as fixed covariates. Random effects included direct additive genetic, animal permanent environmental, and residual effects. Heritability estimates ranged from 0.01±0.03 to 0.17±0.04 (race time); 0.04±0.03 to 0.09±0.04 (final ranking), and repeatability from 0.19±0.02 to 0.37±0.02 (race time) and 0.15±0.02 to 0.23±0.04 (final ranking). Pearson's correlations of estimated breeding values between race time and ranking ranged from -0,027±0.16 to 0.84±0.01. When analyzing the genetic trend for races at a distance of 1,000 m, we observed a decrease in race time over time. However, for races at 1,600 and 2,000 m, a clear pattern of time reduction was not apparent. Race time offers a more promising response to selection, particularly in shorter races. Selecting for race time is expected to have a greater indirect impact on final rank. Genetic trends have revealed limited genetic progress over the years; therefore, it is advisable to reevaluate the current selection process.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105244"},"PeriodicalIF":1.3,"publicationDate":"2024-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fiber composition, in vitro true digestibility, and fiber degradation of hempseed meal compared to mainstream feedstuffs using equine feces as microbial inoculum within the DaisyII Incubator","authors":"R.W. Springer , N.M. Cherry , J.P. Muir , R.H. Raub , K.B. Wellmann , T.N. Jones","doi":"10.1016/j.jevs.2024.105230","DOIUrl":"10.1016/j.jevs.2024.105230","url":null,"abstract":"<div><div>Recent changes in legislation have put hempseed meal (<em>Cannabis sativa</em> L.; HSM) under consideration as a potential livestock feedstuff. Digestibility of HSM has been researched in other species, however, there are little data in horses. The study objective was to determine the in vitro true digestibility (IVTD) of HSM compared to common equine feedstuffs. An in vitro split-split plot design used four geldings (8.5 ± 3.9yr; 558.4 ± 31.0kg; 6.0 ± 0.4 BCS) to investigate the IVTD of HSM compared to alfalfa hay (ALF), Coastal Bermudagrass hay (CBH), soybean meal (SBM), rice bran pellets (RBP), and a commercial concentrate (Bluebonnet Equilene Pellets; EQU). Fresh feces were collected after a 21-d acclimation period and used as incubation inoculum. Dried ground feed samples were incubated in ANKOM filter bags within 2 DaisyII incubators for 48h. Split samples were analyzed for pre-fermentation neutral detergent fiber (NDF). Incubated filter bags were dried and analyzed for NDF to determine IVTD and fiber degradation. Hempseed meal NDF was greater than ALF (<em>P</em> < 0.001), RBP (<em>P</em> < 0.001), and SBM (<em>P</em> < 0.001) but less than CBH (<em>P</em> < 0.001) and tended to be greater than EQU (<em>P</em> = 0.077). The IVTD of HSM was greater than CBH (<em>P</em> < 0.001) but less than all other feedstuffs (<em>P</em> < 0.001). The results indicate that HSM has an NDF and IVTD between ALF and CBH. Due to its lower digestibility and NDF content compared to concentrates, HSM has potential to be used as a fiber source in equine rations.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105230"},"PeriodicalIF":1.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S.C. Chaintoutis, N. Diakakis, Z.S. Polizopoulou, C.I. Dovas
{"title":"Serum protein electrophoretic profile changes in West Nile virus-naturally infected horses","authors":"S.C. Chaintoutis, N. Diakakis, Z.S. Polizopoulou, C.I. Dovas","doi":"10.1016/j.jevs.2024.105232","DOIUrl":"10.1016/j.jevs.2024.105232","url":null,"abstract":"<div><div>Serum protein electrophoresis (SPE) is widely used to evaluate protein changes associated with several pathologies, aiming to assist clinical diagnosis. This study determines, for the first time, SPE profile changes in West Nile virus-naturally infected horses. Sixty horses with different clinical and infection status (encephalitis, asymptomatic infection, and immunologically naïve) were included. Total protein concentrations were determined via an automated biuret method and SPE was performed using a cellulose acetate membrane-based automated system. Statistical analysis revealed significantly higher total protein between both infected horse groups (encephalitis, asymptomatic) and naïve controls. Horses with encephalitis had higher globulin and α2-globulin levels than the other two groups (asymptomatic, naïve), along with lower albumin percentage and albumin-to-globulin ratio. Furthermore, γ-globulin levels were significantly higher in asymptomatically infected compared to control horses. These findings highlight the diagnostic value of SPE profile determination, assisting the clinicians, and providing the grounds for further research.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"144 ","pages":"Article 105232"},"PeriodicalIF":1.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}