V. Jovanović , M. Vučinić , E. Voslarova , K. Nenadović
{"title":"Welfare assessment of stabled horses in five equestrian disciplines","authors":"V. Jovanović , M. Vučinić , E. Voslarova , K. Nenadović","doi":"10.1016/j.jevs.2024.105203","DOIUrl":"10.1016/j.jevs.2024.105203","url":null,"abstract":"<div><div>In Serbia, at present, there is very little information about the welfare of horses. The aim of this study was to identify the main welfare issues likely to be encountered in five different categories of horses (Western riding horses, riding school horses, leisure horses, jumping horses, and endurance horses) kept in stabled housing systems. A total of 50 horses were evaluated using the Animal Welfare Indicator (AWIN) welfare assessment protocol for horses. Identified welfare issues in horses were inadequate box dimensions (52%, 26/50), insufficient quantity of bedding material (34%, 17/50), dirty bedding material (52%, 26/50), integument alterations (68%, 34/50), lameness (16%, 8/50), stereotypic behaviors (28%, 14/50), and negative response to human presence (24%, 12/50). The mean scores for alopecia, skin lesions, hardened spots at the mouth corners, horse grimace scale, and human-animal relationship tests in riding school horses were significantly higher (P<0.05; P<0.001) than for other categories of horses. The leisure horses exercised significantly less often (P<0.001) compared with other categories of horses. Additionally, important risks to welfare, identified across all groups of horses, were insufficient social interaction where 14 horses (28%) were unable to have any visual or physical contact with other horses. The results of this study provide the first analysis and valuable insight into the impact of the individual stable housing system on the welfare of different categories of horses in the Balkan region.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"143 ","pages":"Article 105203"},"PeriodicalIF":1.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Amber T.K. Best , Jessica M. Morgan , Clara L.D. Burgy , Harriet Flynn , Linda S. Barter , Jennifer M. Cassano
{"title":"A blinded crossover study design to evaluate midazolam as an adjunct for equine standing sedation for routine oral examinations","authors":"Amber T.K. Best , Jessica M. Morgan , Clara L.D. Burgy , Harriet Flynn , Linda S. Barter , Jennifer M. Cassano","doi":"10.1016/j.jevs.2024.105201","DOIUrl":"10.1016/j.jevs.2024.105201","url":null,"abstract":"<div><div>This study aimed to evaluate the effects of including midazolam to a common equine standing sedation protocol for routine oral examination. Twelve horses underwent two examinations at least seven days apart. Horses were randomly assigned to receive midazolam intravenously (IV) (0.02mg/kg) or a placebo injection of saline (2-2.5mL IV). Five minutes later, detomidine (0.01mg/kg) and butorphanol (0.01mg/kg) were administered IV and horses were placed in standing stocks. A veterinarian blinded to the treatment protocol used a descriptive scoring system to assess degree of ataxia, acceptance of speculum, chewing on the speculum, headshaking, tongue movement, resistance to palpation, and eye appearance as related to the grimace score. During each examination, additional sedation of IV detomidine (0.006mg/kg) and butorphanol (0.006mg/kg) was administered at the discretion of the blinded practitioner to facilitate safe examination. At the second examination horses received the opposite treatment protocol and, following examination, a routine occlusal adjustment. Scores were compared using JMP software with a repeated measures mixed effects model, treatment as a fixed effect and horse and horse/treatment interaction as random effects. Significance was set at P<0.05. There were no significant differences in any of the single or overall sedation scores between treatment groups or within individual horses (P=0.3). Trends towards improvement of some assessed characteristics of sedation, including decreased tongue movement and less resistance to acceptance of speculum were observed. The use of midazolam may prove beneficial for routine oral examination, as well as other standing procedures, with no obvious undesired side effects.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"143 ","pages":"Article 105201"},"PeriodicalIF":1.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Streptococcus ovis associated abortion in an Icelandic mare","authors":"J.S. Agerholm , P. Damborg , M. Christoffersen","doi":"10.1016/j.jevs.2024.105202","DOIUrl":"10.1016/j.jevs.2024.105202","url":null,"abstract":"<div><div>Streptococci are well-known opportunistic bacterial abortifacients in mares. Colonization of the pregnant uterus is considered to happen after transcervical migration of bacteria from the lower genital tract mucosa. <em>Streptococcus ovis</em> is a pathogen mainly associated with inflammatory lesions in sheep. This species has not been reported in association with disease in horses. In the present case, <em>S. ovis</em> was isolated in monoculture from the lung of an 8-months-old equine fetus and was associated with development of acute suppurative bronchopneumonia, umbilical cord cellulitis and placentitis in the cervical star region of the allantochorion. The mare had been in a pasture together with sheep. One week prior to abortion, a double-guarded uterine swab had been inserted into the cervical canal by a veterinarian, who was unaware of the mare being pregnant. This probably damaged the cervical mucus plug thus allowing <em>S. ovis</em> bacteria to pass the cervical canal and colonize the placenta.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"143 ","pages":"Article 105202"},"PeriodicalIF":1.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142348025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M.A.F. Lopes, E. Hollenbach, E.C. Schliewert, M.A. Murphy, F. Corrêa
{"title":"Closed laceration of the trachea, esophagus and guttural pouches in a mare caused by blunt trauma","authors":"M.A.F. Lopes, E. Hollenbach, E.C. Schliewert, M.A. Murphy, F. Corrêa","doi":"10.1016/j.jevs.2024.105196","DOIUrl":"10.1016/j.jevs.2024.105196","url":null,"abstract":"<div><div>A mare was admitted for progressive swelling of the neck, lethargy and anorexia. Radiography revealed perilaryngeal, peritracheal, and periesophageal emphysema. Endoscopy revealed pharyngeal and tracheal roof collapse, and a small laceration on the trachea. Treatment with antimicrobials, fluids, and flunixin was initiated. To prevent exacerbation of the emphysema, temporary tracheostomy was performed. Clipping for the tracheostomy revealed a hoofprint mark on the ventral neck. Subsequent endoscopies revealed laceration of the esophagus and guttural pouch septum communicating with the visceral compartment of the neck. Four days after admission, an esophagostomy was performed to prevent leakage of ingesta into the neck and allow feeding. Complications occurred: Deep cervical infection requiring surgical drainage; Esophageal impaction with shavings on one occasion causing extensive mucosa erosions; Laminitis managed with restricted physical activity and corrective farriery. Tracheostomy tube removal, esophagostomy tube removal, and patient discharge occurred 10, 36 and 51 days after admission, respectively. The mare did well after discharge and returned to competing in children's showing classes. Blunt trauma to the neck can lacerate the trachea, esophagus and guttural pouches causing emphysema and deep cervical infection, which can be treated with antimicrobials, temporary tracheostomy, temporary esophagostomy, surgical drainage, and supportive care.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"143 ","pages":"Article 105196"},"PeriodicalIF":1.3,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
D. Caivano , M. Cicogna , S. Orvieto , D. Spitale , F. Porciello
{"title":"Quadricuspid aortic valve in a 16-year-old Quarter horse","authors":"D. Caivano , M. Cicogna , S. Orvieto , D. Spitale , F. Porciello","doi":"10.1016/j.jevs.2024.105199","DOIUrl":"10.1016/j.jevs.2024.105199","url":null,"abstract":"<div><div>A 16-year-old horse, 400 kg, male, Quarter horse gelding was examined for a cardiac murmur that had not been previously heard. Physical examination revealed a regularly irregular pulse and a grade III/VI, decrescendo, diastolic murmur with a point of maximum intensity over the left heart base. Base-apex standard electrocardiographic examination at rest showed sinus rhythm with second-degree atrio-ventricular blocks. Echocardiography identified the presence of four aortic valve cusps, two equal larger and two unequal smaller cusps. Color flow Doppler examination showed a diastolic regurgitant jet emerging from the central region of closed aortic valve. Based on clinical and echocardiographic findings a diagnosis of mild to moderate aortic valve insufficiency secondary to quadricuspid aortic valve was made. This report describes a rare congenital heart defect that can be detected by transthoracic echocardiography in the horse.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105199"},"PeriodicalIF":1.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0737080624002053/pdfft?md5=1c0fe421d311ec4ffadd6d81d9208c57&pid=1-s2.0-S0737080624002053-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
José R. Martínez-Aranzales , Mateo Córdoba-Agudelo , Juan E. Pérez-Jaramillo
{"title":"Fecal microbiome and functional prediction profiles of horses with and without crib-biting behavior: A comparative study","authors":"José R. Martínez-Aranzales , Mateo Córdoba-Agudelo , Juan E. Pérez-Jaramillo","doi":"10.1016/j.jevs.2024.105198","DOIUrl":"10.1016/j.jevs.2024.105198","url":null,"abstract":"<div><div>Crib-biting is a stereotyped oral behavior with poorly understood etiology and pathophysiology. The relationship between the gut microbiome and brain function has been described in behavioral disorders such as schizophrenia, depression and anxiety in humans. In horses, studies of behavioral problems and the microbiome are very limited. This study aimed to characterize the fecal microbiome and the predicted functional profile of horses with and without aerophagia. Fecal samples were collected from 12 Colombian Creole Horses of both sexes, divided into two groups: group 1, composed of six horses with crib-biting (3 females and 3 males), average body weight of 330 ± 10 kg, age of 7.0 ± 1.2 years and body condition score (BCS) of 5/9 ± 1 and group 2, consisting of six horses without crib-biting (3 females and 3 males), average body weight of 335 ± 5 kg, age 6.5 ± 1 years and BCS of 6/9 ± 1. From each horse in both groups fecal total DNA was obtained and 16S ribosomal RNA gene amplicons were sequenced to characterize the bacterial community structure. Community structure and differential abundance analyses revealed significant differences between the two conditions (p < 0.05). Specifically, the fecal microbiota at the family level in crib-biting horses, showing a decrease in <em>Bacteroidales</em> and an increase in <em>Bacillota</em> and <em>Clostridia</em>, differed from that of healthy horses without crib-biting, consistent with findings from previous studies. Furthermore, metagenome prediction suggests metabolic profile changes in bacterial communities between both conditions in horses. Further studies are required to validate the role of the microbiota-gut-brain axis in the etiology of crib-biting and other abnormal and stereotyped behaviors.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105198"},"PeriodicalIF":1.3,"publicationDate":"2024-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A novel method for equine embryo transfer from contaminated recipient mares into second healthy recipients for surviving embryos","authors":"M.K. Derbala , A.M. Abu-Seida , A.E. EL-Metwally , H.A.E. Asfour","doi":"10.1016/j.jevs.2024.105200","DOIUrl":"10.1016/j.jevs.2024.105200","url":null,"abstract":"<div><div>Embryo transfer (ET) is regarded as one of the reproductive methods for genetic improvement initiatives in the horse business. The current study aimed to increase the success rate of ET by re-flushing and re-transferring of embryos from contaminated recipient mares into second healthy recipients. The study involved 25 recipient mares that demonstrated embryonic vesicles inside intraluminal fluid (ILF) on ultrasound scanning 2 ± 0.5 days after ET. Seven recipient mares were left without re-flushing and re-transferring of embryos (control group) and 18 recipient mares (treated group) were subjected to re-flushing and re-transferring of the embryos to second healthy and prepared recipients. The retrieved fluid from treated mares was subjected to microbiological and cytological examinations. The results revealed mixed and single infections, as well as polymorphonuclear neutrophils (PMNs). The total bacterial count and cytology findings revealed that all first recipient mares had endometritis. All control mares showed embryonic death at 14 ± 1.1 days (13–15 days) of embryonic age. In treated group, microscopic evaluation of retrieved embryos revealed a well-developed thick capsule in 12/18 (66.7 %) of them and a normal capsule in 6/18 (33.3 %). The pregnancy rate in well-developed capsule embryos was greater (66.7 %) than in typical capsule embryos (33.3 %). This study illustrates the value of swabbing the uterus of the recipient mares during estrus prior to ET and their checking at 2 days after ET. In addition to boosting pregnancy rates in ET commercial programs, our novel method can protect the embryos from the harmful effects of the inflammatory process and help them survive.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105200"},"PeriodicalIF":1.3,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of protein acetylation on capacitation of stallion sperm","authors":"L.H. de Aguiar , C.R.F. Pinto","doi":"10.1016/j.jevs.2024.105195","DOIUrl":"10.1016/j.jevs.2024.105195","url":null,"abstract":"<div><div>Sperm capacitation is considered the main factor limiting conventional in vitro fertilization (IVF) in horses. A recent scientific breakthrough in sperm processing for IVF in horses has resulted in embryos and foals being produced; however, various aspects of the IVF process remain to be fully elucidated. Lysine acetylation has been shown to play a role in sperm capacitation in several species and the objective of this study was to detect and evaluate this process in the horse. Ejaculates of two stallions were collected and incubated in different conditions with deacetylase inhibitors to induce a hyperacetylation state. Although lysine acetylation was successfully detected in all experimental groups, sperm hyperacetylation could not be induced following incubation with deacetylase inhibitors. In addition, no hyperactivation was detected by kinematic sperm evaluation and tyrosine phosphorylation increased only in the positive control group. Treatments with high doses of deacetylase inhibitors increased acrosome reaction indicating a possible connection between induction of acrosome reaction and protein acetylation. Future studies investigating the effect of longer incubation periods with different doses of deacetylase inhibitors are warranted to elucidate the ability of protein acetylation to induce capacitation of stallion sperm.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105195"},"PeriodicalIF":1.3,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Malsa , F. Reigner , M. Riou , A. Gesbert , F. Guégnard , N. Perrot , D. Serreau , G. Fleurance , G. Sallé
{"title":"Evaluation of plant commercial feed additives for equine cyathostomin control","authors":"J. Malsa , F. Reigner , M. Riou , A. Gesbert , F. Guégnard , N. Perrot , D. Serreau , G. Fleurance , G. Sallé","doi":"10.1016/j.jevs.2024.105197","DOIUrl":"10.1016/j.jevs.2024.105197","url":null,"abstract":"<div><div>The increasing emergence of anthelmintic-resistant parasitic isolates prompts us to reassess the management of intestinal strongylosis in horses. Additionally, societal demand is shifting toward reducing the use of chemical treatments, aligning with environmentally-friendly practices and the exploration of natural alternatives. In this context, we provide an initial view of the antiparasitic activity and the effect on immune circulating blood cells of three commercialized plant-based feed additives in ponies. Three treatments, based either on mugwort (<em>Artemisia vulgaris</em>), echinacea (<em>Echinacea purpurea</em>) or curcumin (<em>Curcuma longa</em>) were administrated to 18 (six per treatment) Welsh female ponies naturally infected with cyathostomins to mimic their practical use in farming conditions. Another group of six untreated ponies was used as a control. Fecal egg count (FEC), the larval development percentage and the number of red blood cells, lymphocytes, monocytes, neutrophils, eosinophils and basophils were measured the first and the last day of each treatment, and compared with those characterizing the control group. None of the three treatments showed a significant effect on the studied parameters. Moreover, the efficacy of treatments, measured from the FEC reduction compared to the control group, was weak (≤ 38.6 %). Therefore, these results do not support the practical use of these additives in equine farming, even if the determination of Cohen's d values associated with the three treatments revealed some incidences on FEC and blood immune cell counts, as well as on larval development for mugwort.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105197"},"PeriodicalIF":1.3,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S073708062400203X/pdfft?md5=729e0183a4eb78e5276d375298c0cbfb&pid=1-s2.0-S073708062400203X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142289001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Caure , P. Dendauw , L. Thesee , E. Villedey , A. Malinvaud , M. Cousty , V. Prie , K. Horan , R. Weller
{"title":"Effects of different shoeing conditions on equine cervical and back kinematics during walking and trotting on a soft surface","authors":"S. Caure , P. Dendauw , L. Thesee , E. Villedey , A. Malinvaud , M. Cousty , V. Prie , K. Horan , R. Weller","doi":"10.1016/j.jevs.2024.105194","DOIUrl":"10.1016/j.jevs.2024.105194","url":null,"abstract":"<div><p>There is a paucity of scientific data on the effect of shoeing on equine neck and back kinematics during locomotion over commonly used sand training surfaces. A better appreciation of how alterations at hoof-ground interface influence equine upper body movements is relevant for improving horse's health and performance. Our objectives were to determine the effects of different shoeing conditions on equine neck and back kinematics at walk and trot in straight line over sand. Two-dimensional kinematic video analysis was performed under seven shoeing conditions: front feet shod with aluminum shoes and hind feet with steel racehorse shoes (REFSHOD), front aluminum shoe and hind feet unshod (FORESHOD), front feet unshod and hind steel race shoes (HINDSHOD), all four feet unshod (UNSHOD), front feet shod in combination with hind egg bar shoes (hEGGBAR), hind wide toe shoes (hTOE) and hind reverse shoes (hREVERSE). Data indicated that joint angles in the cervicothoracic junction were four times more likely to be significantly affected by the shoeing condition than in the back and sacrum. FORESHOD largely modifies the kinematics in comparison to REFSHOD or UNSHOD, with respectively a 6-11±1-2° (<em>P</em><0.001) increased cervicothoracic extension at walk and trot, and a 3-4±1° (<em>P</em><0.05) increased thoracolumbar flexion at trot. In comparison to REFSHOD, hEGGBAR, hTOE and hREVERSE induce a 5-7±1-2° (<em>P</em><0.05) increased cervicothoracic extension at trot and walk respectively, and UNSHOD induced cervicothoracic flexion at trot (6±2°, <em>P</em><0.05). In conclusion, shoeing conditions impact equine neck and back position, which should be considered during clinical examination, rehabilitation and training.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105194"},"PeriodicalIF":1.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142239832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}