Alisa C. Herbst, Kyle S. Hartmann, Diana Orban Brown, Karyn Malinowski
{"title":"2022 Equine community needs assessment survey","authors":"Alisa C. Herbst, Kyle S. Hartmann, Diana Orban Brown, Karyn Malinowski","doi":"10.1016/j.jevs.2024.105193","DOIUrl":"10.1016/j.jevs.2024.105193","url":null,"abstract":"<div><p>To stay up-to-date with the demographics, interests, and needs of the equine community, equine extension programs commonly conduct so called “needs assessment surveys”. The main goals of the 2022 Rutgers Equine Science Center (ESC) needs assessment survey were to: 1) characterize the equine community, 2) identify information sources used by the equine community, 3) assess the usefulness of selected equine-related services and programs, and 4) determine the perceived importance of selected equine-related issues. An anonymous online survey was distributed through the Rutgers ESC's email list, a press release, and social media. Analysis-eligible surveys were received from <em>n</em> = 493. Most participants were female (92.7 %), 36-60 years of age (48.4 %), white (88.8 %), and horse owners (87.8 %). The top three sources of equine-related information used were 1) personal knowledge (95.7 %), 2) the veterinarian (93.7 %), and 3) the farrier (83.7 %). The survey showed that the provision of horse health information is the most useful service that the Rutgers ESC specifically can provide (79.3 %). This service may also be most useful for other extension programs, but a follow-up study is needed for clarification. The five equine-related issues most often considered to be extremely important were “Lameness and Soundness” (76.5 %), “Ethical Use of Horses” (73.2 %), “Nutrition” (63.9 %), “Right to Farm” (56.8 %), and “Care of Old Horses” (55.6 %). The survey provided insights into the current demographics of the equine community, highlighted personal knowledge, veterinarians and farriers as important information sources, and elucidated “Lameness and Soundness” as the most important equine-related issue.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105193"},"PeriodicalIF":1.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0737080624001990/pdfft?md5=ee2944a13e2d7780fb32c8ec19c71b74&pid=1-s2.0-S0737080624001990-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142271205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B.S. Kerley , P. Harris , K. Lea , M. McClendon , A.A. Adams
{"title":"Effects of storage-handling methods on nutrient analysis of fresh-forage samples","authors":"B.S. Kerley , P. Harris , K. Lea , M. McClendon , A.A. Adams","doi":"10.1016/j.jevs.2024.105182","DOIUrl":"10.1016/j.jevs.2024.105182","url":null,"abstract":"<div><p>Forage low in nonstructural carbohydrates (NSC) is recommended for insulin dysregulated (ID) horses, indicating the importance of an accurate forage analysis. However, it remains to be fully understood how handling forage samples pre-analysis impacts nutrient values. The objective of this study was to compare the effect of fresh-forage storage-handling methods: microwave-oven (MO; 9kw; 70s then stored at -20℃), room temperature (20℃), 3℃, -20℃, and -80℃. Subsamples collected from a mixed-grass pasture and a ryegrass plot were analyzed for crude protein (CP), water soluble carbohydrates (WSC), ethanol soluble carbohydrates (ESC), starch and NSC (starch and WSC). Forage samples were stored for two different time periods (24h vs 1wk) prior to being shipped on ice to a commercial laboratory for wet chemistry (WC) and near-infrared spectroscopy (NIR) analysis. Mixed grass stored at RT showed a reduction in WSC (P=0.009), ESC (P=0.001) and NSC (P=0.006) from 24h to 1wk. Similarly, ESC and starch of the ryegrass and CP of mixed grass decreased after 1wk, but only when stored at -80℃ (P=0.007; P=0.001; P=0.02). Additionally, over time, CP of ryegrass and ESC of mixed grass became higher when stored at -20℃ and MO, respectively (P=0.02; P=0.03). From this study, in order to limit metabolic changes and provide accurate nutrient composition results, practically fresh forage that cannot be quickly analyzed should be transported on ice post collection to a storage location then immediately refrigerated where it can be kept up to one week prior to being shipped on ice for analysis.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105182"},"PeriodicalIF":1.3,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S.P. Daniels , E.J. Whiteside , S. Martin , M.J.S. Moore-Colyer , P. Harris
{"title":"Straight from the horse's mouth: The effect of different feedstuffs on oral pH in horses and ponies","authors":"S.P. Daniels , E.J. Whiteside , S. Martin , M.J.S. Moore-Colyer , P. Harris","doi":"10.1016/j.jevs.2024.105181","DOIUrl":"10.1016/j.jevs.2024.105181","url":null,"abstract":"<div><p>Feedstuffs, especially ensiled forage, may be detrimental to equid oral health by exposing the oral cavity to low pH. This study aimed to identify if salivary pH was altered by 1) a range of different feedstuffs and (2) specifically by feeding haylages with differing nutrient profiles. Two studies were conducted. The first measured oral pH following five feedstuffs, (hay, haylage, unmolassed sugarbeet pulp, unmolassed alfalfa chaff and rolled oats), tested individually over five days. Saliva (≥1 ml) was collected in triplicate, prior to feeding, directly after ingesting 500 g of each feedstuff, then 15 min and 30 min post-prandially. Oral pH was determined (pH meter) within 10 min of collection. In study two, eight ponies, were fed as their total diet, four different haylages over four 15-day periods. Saliva was collected, prior to feeding and immediately after ingesting 500 g of forage on day 1, day 6, and day 12 of each period. Samples were collected and analysed as per study one. All data were analysed by repeated measures ANOVA, and in study two linear regression was used to attempt to predict nutrients that influenced oral pH. All statistics were conducted in Genstat 20 th Ed. Only feeding unmolassed sugarbeet caused a reduction (p<0.001) in oral pH. There were differences in oral pH depending on the type of haylage fed in study two but at all times oral pH post-feeding was the same or greater than basal pH. These studies suggest any feed associated modulation of oral pH in horses may only be short-lived and quickly buffered by saliva. However, these studies only reflect oral pH within the oral cavity around the feeding occasion and may not reflect gingival pH or the effects of different feeds over longer time periods.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105181"},"PeriodicalIF":1.3,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142145729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dale Kelley , Stephanie Walbornn , Christine Bartley , Jamie Kaczor , Christopher Premanandan , Nathaniel Newton , Robert Holland , Maria Schnobrich
{"title":"Intra-uterine injection of amnion-derived acellular bioscaffold product in mares, a description of systemic and intra-uterine effects over 21 days","authors":"Dale Kelley , Stephanie Walbornn , Christine Bartley , Jamie Kaczor , Christopher Premanandan , Nathaniel Newton , Robert Holland , Maria Schnobrich","doi":"10.1016/j.jevs.2024.105180","DOIUrl":"10.1016/j.jevs.2024.105180","url":null,"abstract":"<div><p>Amnion-derived acellular bioscaffold (ADABP) products demonstrate interesting anti-inflammatory and healing properties which could be beneficial for intrauterine use. The objective of this study was to evaluate the safety of intrauterine injection of ADABP on systemic and uterine health. The study design randomly assigned subjects to one of two groups, control mares (<em>n</em> = 3) which received 3 mL injection of sterile saline in the base of each uterine horn, and treatment mares (<em>n =</em> 9) which received 3 mL of ADABP in the base of one uterine horn and 3 mL injection of sterile saline in the base of the other uterine horn. The leukogram had no significant effect of group and no group by day interaction. The serum biochemistry panel had no effect of group on any of the parameters examined. There were no significant differences in uterine culture or uterine biopsy results. The data suggests intrauterine injection of ADABP has no negative systemic or uterine effects.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105180"},"PeriodicalIF":1.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142098799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P.E.M. Sibthorpe, D.M. Fitzgerald, M.N. Sillence, M.A. de Laat
{"title":"Studies in vitro of equine intestinal glucagon-like peptide-2 secretion","authors":"P.E.M. Sibthorpe, D.M. Fitzgerald, M.N. Sillence, M.A. de Laat","doi":"10.1016/j.jevs.2024.105179","DOIUrl":"10.1016/j.jevs.2024.105179","url":null,"abstract":"<div><p>Equine insulin dysregulation (ID) is a significant metabolic problem because the hyperinsulinaemia that develops increases the animal's risk of developing laminitis, a debilitating foot condition. The role of gastrointestinal factors, such as incretin hormones, in the pathogenesis of ID and hyperinsulinaemia in horses is poorly understood, particularly in comparison to other species. Glucagon-like peptide-2 (GLP-2) is an intestinotrophic peptide released from L cells in the gastrointestinal tract and is implicated in metabolic dysfunction in other species. The aim of this study <em>in vitro</em> was to establish basic physiological understanding about intestinal secretion of GLP-2 in horses. Basal and glucose-stimulated GLP-2 secretion was measured in post-mortem tissue samples from the duodenum, jejunum, and ileum. We observed that GLP-2 secretion was minimal in samples from the duodenum compared to the jejunum and ileum (5-9-fold higher; P < 0.05). Furthermore, GLP-2 secretion was not responsive to glucose stimulation in the ileum or duodenum but was responsive to glucose in the jejunum. This effect in the jejunum was inhibited by 30 % (P = 0.02) using phlorizin, a selective sodium-glucose cotransporter-1 (SGLT-1) inhibitor, and by 38 % (P = 0.04) using phloretin, a non-selective SGLT-1/GLUT-2 inhibitor. The localisation of glucose-responsive GLP-2 secretion in the jejunum might be relevant to the development of post-prandial hyperinsulinaemia. This study has provided data on GLP-2 secretion from the equine small intestine that will enable more complex and dynamic studies on the pathogenesis of ID.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105179"},"PeriodicalIF":1.3,"publicationDate":"2024-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0737080624001850/pdfft?md5=06082b63f6d81e4c63ba3aaab452c8c7&pid=1-s2.0-S0737080624001850-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Myriah Albrecht , Katherine Cole MacGillivray , Beatriz Juez , Russell Freeland
{"title":"Esophageal perforation diagnosis, treatment, complications, and outcome in a Standardbred colt","authors":"Myriah Albrecht , Katherine Cole MacGillivray , Beatriz Juez , Russell Freeland","doi":"10.1016/j.jevs.2024.105177","DOIUrl":"10.1016/j.jevs.2024.105177","url":null,"abstract":"<div><p>A 4.5-month-old Standardbred colt presented for neck swelling and fever. Endoscopy and contrast radiography identified a full thickness esophageal perforation. Surgical intervention with fasciotomies and placement of an esophageal feeding tube was elected. Intensive postoperative treatment was carried out with broad-spectrum antibiotics, gastroprotectants and probiotics, calculated feeding plan, esophagostomy and fasciotomy site lavages, care, and cleaning. Complications included recurrent bilateral pneumothorax and development of an incomplete fistula at the esophagostomy site following removal of the feeding tube, necessitating primary closure. One year following presentation, the colt appears to have made a full recovery with an excellent body and muscle condition score and undergoing athletic training. Anticipated price of sale as a yearling was achieved. This study highlights a viable treatment route for youngstock with esophageal perforation that necessitate an esophagostomy. Despite intensive dietary needs of growing foals, appropriate nutritional requirements and normal growth rates can be achieved in such cases.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105177"},"PeriodicalIF":1.3,"publicationDate":"2024-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142073005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Damone , L. Bass , B. Gadomski , S. Rao , C. Frank , V.J. Moorman
{"title":"Biomechanical and histological variables differ by site but not by lameness in equine digital cushion samples from forelimbs","authors":"J. Damone , L. Bass , B. Gadomski , S. Rao , C. Frank , V.J. Moorman","doi":"10.1016/j.jevs.2024.105178","DOIUrl":"10.1016/j.jevs.2024.105178","url":null,"abstract":"<div><p>The equine digital cushion (DC) has been a poorly understood structure regarding its mechanical properties and composition. The objective of this study was to develop a sampling technique and to compare the biomechanical and histologic properties of DC between lame and non-lame forelimbs. Both forefeet from horses with induced carpal lameness were radiographed prior to humane euthanasia. Radiographs were used to guide sample collection of two, post-mortem, midline DC samples, palmar and dorsal, via an 8mm biopsy punch. Samples were subjected to compressive testing to determine elastic modulus. Histological evaluation was used to quantify collagen, adipose, ground substance, elastic fibers, and blood vessels. Comparisons of variables between palmar and dorsal DC sites and lame and non-lame limbs were made using student's t-tests or Wilcoxon signed rank tests with <em>P</em> < 0.05. There were no differences in histologic or biomechanical properties in DCs of lame and non-lame forelimbs. The dorsal DC sampling site had a significantly higher median elastic modulus (median: 0.054 MPa, range: 0.001 – 2.110 MPa) and a larger median percentage of ground substance (median: 15 %, 95 % CI: 3 – 30 %) compared to the palmar DC site (elastic modulus: median: 0.023 MPa, range: 0 – 0.576 MPa; ground substance: median 5 %, 95 % CI: 3 – 25 %) (<em>P</em> = 0.03, <0.001; respectively). This study demonstrated that adequate samples could be collected from the DC for biomechanical and histological analysis. Differences in elastic modulus and ground substance between palmar and dorsal samples may reflect different roles of these sites within the DC.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"142 ","pages":"Article 105178"},"PeriodicalIF":1.3,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142055784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L.T. Johns , M.P. Smythe , L.S. Dewberry , E.A. Staiger , K. Allen , S.A. Brooks
{"title":"Digital video analysis reveals gait parameters that predict performance in the jumping test phase of three-day eventing","authors":"L.T. Johns , M.P. Smythe , L.S. Dewberry , E.A. Staiger , K. Allen , S.A. Brooks","doi":"10.1016/j.jevs.2024.105166","DOIUrl":"10.1016/j.jevs.2024.105166","url":null,"abstract":"<div><p>In international equestrian sport, visual inspections assess gait and lameness to protect the welfare of performance horses during competition. Horses competing internationally in three-day eventing must pass two mandatory inspections (pre-competition and post-cross country) before attempting the final phase: the jumping test (JT). We hypothesized that digitally quantifying objective gait parameters captured during the two mandatory inspections will identify locomotor characteristics that predict success during the jumping test. Utilizing the DeepLabCut (DLC) software package for labeling of anatomical landmarks and a custom analysis pipeline we calculated gait parameters for 194 competition horses at the trot. During the pre-competition inspection, relative trot speed was significantly associated (P = 0.0060, GLMM), and the forelimb travel trended towards significance (P =0.0800, GLMM), with achieving a clear round in the later jumping test. Post-cross country, the forelimb travel significantly predicted JT results (P = 0.0188, GLMM). As our parameters are scaled for body size, these parameters may indicate conformational characteristics for superior jumping ability and overall athletic fitness. Within each competitive effort, comparisons of the post-cross country and pre-competition observations revealed that the change in speed and duty factor were significantly different in the group that accrued jumping faults (P = 0.00376 and P = 0.02430, GLMM), perhaps capturing locomotor signs of exercise fatigue. Further work employing these approaches to better understand competition performance will encourage the use of objective measures to protect sport horse welfare, as well as provide an advantageous tool for gait evaluation in the horse.</p></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"141 ","pages":"Article 105166"},"PeriodicalIF":1.3,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}