Journal of Equine Veterinary Science最新文献

{"title":"Morphometric parameters, metabolic profile and musculoskeletal alterations in Criollo horses competing in the morphology trial","authors":"Mariana A. Mousquer ,&nbsp;Cahuê F. R. Paz ,&nbsp;Leandro A. Rafael ,&nbsp;Camila G. Wendt ,&nbsp;Bruna R. Curcio ,&nbsp;Rafaela P. de Souza ,&nbsp;Isadora P. O. dos Santos ,&nbsp;Rafaela B. da Silva ,&nbsp;Vitória Müller ,&nbsp;Carlos Eduardo W. Nogueira","doi":"10.1016/j.jevs.2025.105703","DOIUrl":"10.1016/j.jevs.2025.105703","url":null,"abstract":"<div><h3>Background</h3><div>Obesity is common in some horse breeds, raising concerns about health and performance. Criollo horses in morphological events often undergo intensive management promoting early adiposity.</div></div><div><h3>Aims/objectives</h3><div>This study aimed (1) to assess morphometric traits, metabolic profile, hoof morphology, and tarsal joint alterations in Criollo horses participating in morphological trials; (2) to evaluate sex-related differences; and (3) the associations between morphometric measurements, hoof morphology, and tarsal osteoarthritis grades.</div></div><div><h3>Methods</h3><div>Fifty-five horses (30 mares, 25 stallions) were evaluated for body condition score (BCS), cresty neck score (CNS), morphometric measurements and metabolic parameters including cholesterol, lipoproteins, leptin, adiponectin and muscle enzymes. Forelimb hooves (<em>n</em> <em>=</em> <em>49</em>) radiographs were analyzed for morphology and tarsal joints (<em>n</em> <em>=</em> <em>48</em>) radiographs were analyzed for the presence of degenerative alterations.</div></div><div><h3>Results</h3><div>A high prevalence of overweight horses (BCS ≥7) was observed. Males had greater neck circumference (<em>p</em> &lt; 0.05), females had higher body fat percentage (<em>p</em> &lt; 0.05). Distal phalanx descent correlated positively with body fat (<em>r</em> <em>=</em> <em>0.31, P</em> <em>=</em> <em>0.01</em>) and tailhead fat (<em>r</em> <em>=</em> <em>0.31, P</em> <em>=</em> <em>0.01</em>). Distal lamellar zone distance positively correlated with body fat <em>(r</em> <em>=</em> <em>0.34; P</em> <em>=</em> <em>0.009</em>) and tailhead fat <em>(r</em> <em>=</em> <em>0.34; P</em> <em>=</em> <em>0.009)</em>, and hoof angle correlated negatively with neck fat (<em>r=-0.41; P</em> <em>=</em> <em>0.02</em>). Males showed longer P2 (<em>p</em> &lt; 0.05). All horses had tarsal radiographic lesions; females exhibited higher lesion scores than males (<em>p</em> = 0.005).</div></div><div><h3>Conclusion</h3><div>These findings highlight the prevalence of overweight animals in Criollo breed morphological trials and the occurrence of tarsal joint degeneration in this population. The association between hoof measurements and adiposity variables emphasizes the potential impact of elevated body condition on musculoskeletal alterations and laminitis development.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105703"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145069774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agreement between subjective evaluations and a markerless AI-based gait analysis system during lungeing assessment in traditional racehorses","authors":"F. Meistro,&nbsp;M.V. Ralletti,&nbsp;R. Rinnovati,&nbsp;A. Spadari","doi":"10.1016/j.jevs.2025.105704","DOIUrl":"10.1016/j.jevs.2025.105704","url":null,"abstract":"<div><h3>Background</h3><div>Subjective lameness evaluation during lungeing is routinely performed in equine practice, but its consistency remains limited, especially in cases of mild or complex asymmetry.</div></div><div><h3>Aims</h3><div>This study aimed to assess the agreement between subjective gait evaluations and a markerless AI-based gait analysis system (OAI-MS) in traditional racehorses during lungeing. Intra- and inter-observer agreement of subjective evaluations was also investigated.</div></div><div><h3>Methods</h3><div>24 traditional racehorses were assessed during routine pre-race inspections (T0) while trotting on a soft surface. Two experienced equine clinicians independently evaluated each horse on both reins using the AAEP 0–5 scale; scores were then converted to a 3-level ordinal scale (0 = sound, 1 = mild, 2 = severe). Simultaneously, gait data were collected using the OAI-MS. A subset of 10 horses was re-evaluated after 10 days (T1) to assess short-term repeatability of the OAI-MS. Video-based reassessment (T2) was used to evaluate intra-observer agreement. Agreement was calculated using weighted Cohen’s and Fleiss’ kappa. <em>p</em> &lt; 0.05.</div></div><div><h3>Results</h3><div>Inter-observer agreement ranged from κ = –0.20 to 0.36. Agreement between subjective evaluators and the OAI-MS ranged from slight to moderate (κ = 0.13–0.47). Intra-observer agreement was fair (κ ≈ 0.22), and OAI-MS repeatability reached κ = 0.43. Agreement was higher for forelimbs than hindlimbs. Most discrepancies were of low magnitude.</div></div><div><h3>Conclusion</h3><div>Subjective gait evaluations during lungeing showed limited agreement. The OAI-MS demonstrated moderate repeatability, supporting its usability in the field and its potential role as a complementary tool in clinical decision-making, particularly when asymmetries are mild or disagreement occurs.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105704"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145191545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro and in vivo effects of Acacia mearnsii De Wild extract for cyathostomin control in horses","authors":"G.L.S. Silva ,&nbsp;U.A. Curcio ,&nbsp;J.A. Boufleur ,&nbsp;D.G.L. Borges ,&nbsp;M.T.M. Nakatani ,&nbsp;M.G. Freitas ,&nbsp;F.A. Borges ,&nbsp;F.J.C. Faria ,&nbsp;R.H.P. Silva ,&nbsp;G.L. Franco","doi":"10.1016/j.jevs.2025.105686","DOIUrl":"10.1016/j.jevs.2025.105686","url":null,"abstract":"<div><h3>Introduction</h3><div>Anthelmintic resistance has led to the use of organic extracts as alternative methods of parasite control.</div></div><div><h3>Objectives</h3><div>The study aimed to assess the effects of <em>Acacia mearnsii</em> extract (tannin) on the control of cyathostomins in horses, both <em>in vitro</em> and <em>in vivo</em>.</div></div><div><h3>Materials and methods</h3><div>Thirty Pantaneiro horses naturally infected with cyathostomins were sourced from two distinct farms, designated as Farms A and B. At the start of the study, all third-stage larvae were cyathostomins, and an egg hatchability test (EHT) was performed using fecal samples from horses on both farms. Two randomized 28-day field studies were conducted for the fecal egg count reduction test (FECRT) with evaluations on days 0, 7, 14, 21, and 28. On days 0 and 28, body weight (BW), body condition score (BCS), and blood samples for hematological and biochemical analysis were also collected. The horses diet included panicum grass pasture, 1 kg of concentrate, 40 g of liquid sugarcane molasses (Control), and 25 g of <em>A. mearnsii</em> extract.</div></div><div><h3>Results</h3><div>The EC50 values were 13.2 mg/mL (95 % confidence interval [CI]: 11.8–14.7 mg/mL) and 14.9 mg/mL (95 % CI: 13.3–16.9 mg/mL) for Farms A and B, respectively. The FECRT showed no effect (<em>P</em> &gt; 0.05) between the control and treated groups, with mean values of 3.09% and 3.18%, respectively. Blood and hematological parameters also showed no differences (<em>P</em> &gt; 0.05) between treatments.</div></div><div><h3>Conclusion</h3><div>The tannin extract reduced egg hatchability in vitro, but did not lower fecal egg counts in naturally infected horses during the in vivo trial.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105686"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144992736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Readers Information","authors":"","doi":"10.1016/S0737-0806(25)00378-8","DOIUrl":"10.1016/S0737-0806(25)00378-8","url":null,"abstract":"","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105720"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145462754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic composition of gastric reflux and small intestinal fluid in horses: implications for fluid replacement","authors":"G.C. Paganelli,&nbsp;H.C. Schott II","doi":"10.1016/j.jevs.2025.105683","DOIUrl":"10.1016/j.jevs.2025.105683","url":null,"abstract":"<div><h3>Background</h3><div>Small intestinal (SI) distension and gastric reflux (GR) are common in horses with mechanical and functional ileus. Removal of GR results in fluid/ion losses.</div></div><div><h3>Aims/objectives</h3><div>1) Determine the capacity of healthy SI; 2) measure ion concentrations in normal SI fluid, GR, or fluid from SI undergoing resection. The authors hypothesized that Na⁺ and Cl^- concentrations would be lower and higher, respectively, in GR as compared to SI fluid from healthy horses, while there would be little difference in K⁺ concentration.</div></div><div><h3>Methods</h3><div>Observational study: two to 15 meters of SI from 15 horses were distended with water to 6-7 cm in diameter. Volume drained was measured to determine capacity. Ion concentrations were measured in SI fluid from healthy horses (<em>n</em>=10), GR (<em>n</em>=11), and strangulated bowel (<em>n</em>=2).</div></div><div><h3>Results</h3><div>Normal SI had a fluid capacity of 2.8 ± 0.6 L/m. Na⁺, K⁺, and Cl^- concentrations were 100 ± 6, 22 ± 5, and 66 ± 30 mmol/L, respectively, in normal SI fluid; 77 ± 16, 16 ± 11, and 96 ± 25 mmol/L, respectively, in GR; and 116 ± 11, 12 ± 5, and 43 ± 27 mmol/L, respectively, in fluid from strangulated SI. Concentrations of Na⁺ were lower (<em>P</em>&lt;0.01) and Cl^- greater (<em>P</em>&lt;0.03) in GR than in normal SI fluid or fluid from strangulated SI, while all fluid K⁺ concentrations were 3-5-fold greater than serum K⁺ concentration.</div></div><div><h3>Conclusion</h3><div>These data can be used to estimate water and ion losses that occur with SI disorders to implement appropriate fluid therapy plans.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105683"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fistulous withers causing spinal epidural abscess in a Thoroughbred mare","authors":"Serena Ceriotti ,&nbsp;Stuart Clark-Price ,&nbsp;Robert Cole ,&nbsp;Alana Kramer ,&nbsp;Maninder Sandey ,&nbsp;Mariano Mora","doi":"10.1016/j.jevs.2025.105680","DOIUrl":"10.1016/j.jevs.2025.105680","url":null,"abstract":"<div><div>This case describes a 15-year-old Thoroughbred mare with fistulous withers that progressed to vertebral osteomyelitis and spinal epidural abscess (SEA), a severe complication that has yet to be reported. Initially treated with antibiotics and anti-inflammatory medication, the mare developed acute hindlimb ataxia and spastic paresis, suggesting spinal cord compression at T3-L3. Diagnostic imaging revealed vertebral fractures and abscessation, but vertebral canal involvement could not be confirmed. Despite aggressive medical therapy, including antimicrobials, corticosteroids, and neuroprotective agents, the mare’s neurological condition deteriorated, leading to euthanasia. Post-mortem findings confirmed an extensive paraspinal abscess that had extended into the epidural space, causing severe spinal cord compression. The isolated pathogen, <em>Escherichia coli</em>, is commonly associated with chronic fistulous withers. This case underscores the importance of considering SEA as a diagnostic differential in horses with chronic paraspinal infections that develop acute neurological decline. Due to diagnostic and treatment limitations in equine medicine, prognosis remains guarded, with early surgical intervention and targeted antimicrobial therapy likely being crucial for better outcomes.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105680"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144956604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrinsic characteristics of donkeys that affect drug treatment","authors":"Ignacio Lizarraga ,&nbsp;Fernanda Castillo-Alcala","doi":"10.1016/j.jevs.2025.105700","DOIUrl":"10.1016/j.jevs.2025.105700","url":null,"abstract":"<div><div>Donkeys have contributed to the development and livelihoods of humans for almost 7,000 years. They have been used for traction and transportation, kept as pets, utilized in animal-assisted therapies, or farmed as food-producing animals. Over the past two decades, significant progress has been made in bridging the knowledge gap in veterinary medicine between donkeys and horses. Veterinary practitioners are now more aware that donkeys have important behavioral, anatomical, and physiological characteristics that distinguish them from horses. These peculiarities should be considered when treating donkeys. For instance, donkeys are highly efficient in compartmentalizing and conserving water and metabolizing xenobiotics via cytochrome P450 enzymes. This affects the selection of drugs and their dosages. Estimating live weight and recognising and assessing pain accurately are also important factors when instituting pharmacological treatments for donkeys. Therapeutic regimens have been established for some drugs in donkeys, but many are still directly extrapolated from horses. Because donkeys are classified as a minor species, there are relatively few medications approved for their use. In addition, some cultures also consider donkeys as to have lower economic value than horses, which may preclude donkeys from receiving appropriate veterinary care. Veterinary education on donkey-specific topics would improve the health and ultimately the welfare of these animals. This review explores the characteristics of donkeys and offers guidance on their care and treatment, aiming to enhance their overall quality of life.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105700"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145053859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutation rate and spectrum of germline de novo mutations in a closed population of Thoroughbred horses","authors":"Risako Furukawa ,&nbsp;Teruaki Tozaki ,&nbsp;Keijiro Mizukami ,&nbsp;Yusuke Iwasaki ,&nbsp;Koki Kawate ,&nbsp;Mio Kikuchi ,&nbsp;Taichiro Ishige ,&nbsp;Yukihide Momozawa ,&nbsp;Emiko Fukui ,&nbsp;Hironaga Kakoi","doi":"10.1016/j.jevs.2025.105682","DOIUrl":"10.1016/j.jevs.2025.105682","url":null,"abstract":"<div><h3>Background</h3><div>Thoroughbreds have been maintained as a closed breed for over 300 years since the crossbreeding between Arabian stallions and English mares. Despite interest in germline <em>de novo</em> mutations across species, their frequency in horses, particularly in Thoroughbreds, remains largely unexplored.</div></div><div><h3>Aims/objective</h3><div>This study aimed to identify <em>de novo</em> mutations in Thoroughbreds and estimate their frequency within a genetically closed population.</div></div><div><h3>Methods</h3><div>We performed deep whole-genome sequencing (≥230× depth, 150 bp paired-end reads) and Sanger validation in a Thoroughbred trio (sire, dam, and foal). Reads were aligned to the EquCab3.0 reference genome, and variants in regions with sequencing depth ≥20 (covering 99.6 % of the genome) were analyzed for Mendelian inconsistencies.</div></div><div><h3>Results</h3><div>A total of 48 <em>de novo</em> mutations were identified, comprising 46 single nucleotide substitutions and 2 deletions, none of which were found in public variant databases. Of these, 18 were of paternal origin and 6 were of maternal origin (<em>P</em> &lt; 0.05). The estimated mutation rate was 9.59 × 10⁻⁹, which is in a comparable range to that reported in humans. One mutation (chr7:g.2084761G&gt;<em>A</em>) introduced a nonsense variant in the zinc finger protein 77 gene, which encodes a putative transcriptional regulator.</div></div><div><h3>Conclusions</h3><div><em>De novo</em> mutations were identified in the Thoroughbred genome, and the mutation rate is consistent with estimates from other mammalian studies. A significant bias toward paternal origin was observed. Our findings suggest that germline <em>de novo</em> mutations are a source of novel genetic variation in Thoroughbreds, even within a genetically closed population.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"154 ","pages":"Article 105682"},"PeriodicalIF":1.6,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144989807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphometric characteristics of quarter horses in barrel racing events: impacts on performance and competitive success","authors":"Giovanna Polo Freitag ,&nbsp;Luis Gustavo Freitag de Lima ,&nbsp;Luiz Ernandes Kozicki ,&nbsp;Fernando Andrade Souza ,&nbsp;Eriklis Nogueira ,&nbsp;Leonir Bueno Ribeiro","doi":"10.1016/j.jevs.2025.105676","DOIUrl":"10.1016/j.jevs.2025.105676","url":null,"abstract":"<div><h3>Aims</h3><div>This study investigated the influence of morphometric characteristics on performance in Quarter Horses competing in barrel racing.</div></div><div><h3>Methods</h3><div>A total of 125 horses were assessed using 28 linear and angular body measurements derived from standardized left-side photographs captured via smartphone and analyzed with open-source software.</div></div><div><h3>Results</h3><div>Measurements were subjected to exploratory factor analysis, extracting six principal components (forequarters, hindquarters, midsection, posterior angles, scapular traits, age and weight), which together explained 83.0 % of the morphological variance. Forequarter morphology, including withers height and limb lengths, accounted for 37.4 % of the variation in race performance. Hierarchical cluster analysis grouped horses by trial times, revealing that animals with faster times exhibited significantly larger ribcage dimensions, longer scapulae, and greater coxofemoral angles. A multivariate regression model incorporating the principal components predicted individual potential trial times with high accuracy (R² = 0.6848; <em>P</em> = 0.0388), and comparisons with actual times confirmed the model’s validity. Age and body weight were also relevant, with younger, well-muscled horses demonstrating superior performance.</div></div><div><h3>Conclusion</h3><div>Forequarter conformation, scapular inclination, and indicators of respiratory and muscular capacity were strong predictors of barrel racing success in Quarter Horses, and image-based morphometric analysis was a valuable tool for performance prediction and selection in this study.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"153 ","pages":"Article 105676"},"PeriodicalIF":1.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma Activin A concentrations are not a useful biomarker for detecting insulin dysregulation and predicting laminitis risk in ponies","authors":"C.J. McGuire ,&nbsp;E.J. Knowles ,&nbsp;P.A. Harris ,&nbsp;N.J. Menzies-Gow","doi":"10.1016/j.jevs.2025.105660","DOIUrl":"10.1016/j.jevs.2025.105660","url":null,"abstract":"<div><h3>Background</h3><div>Circulating insulin concentrations are used to quantify risk of laminitis in currently non-laminitic ponies. Basal serum activin A (AA) concentration has previously been positively correlated with serum insulin concentrations 60 minutes (T60) following an oral sugar test (OST) in ponies with equine metabolic syndrome. Therefore, circulating AA might be a useful marker for insulin dysregulation (ID) and laminitis risk.</div></div><div><h3>Objectives</h3><div>To explore the relationship between circulating AA concentrations at baseline (T0) and T60 in ponies that developed laminitis within six months and non-laminitic ponies.</div></div><div><h3>Methods</h3><div>Case-control study. Forty-three ponies that developed laminitis (PLP) during a four-year surveillance period were selected from a larger cohort; 43 ponies from the same cohort that remained non-laminitic (NLP) were selected as controls. Plasma AA concentrations were measured using a validated ELISA at T0 and T60 and compared between groups at both time points using a Wilcoxon signed ranks test. Correlations with other previously measured metabolic markers were investigated using Spearman‘s rank correlation coefficients.</div></div><div><h3>Results</h3><div>There was a significant difference between T0 plasma AA (median 8.8; interquartile range [6.2, 13.1]ng/mL) and T60 (13.1; [8.8, 20.1]ng/mL) within the NLP group (P= &lt;0.001) and between plasma AA at T0 (7.4 [5.5, 12.4]ng/mL) and T60 (12.3 [6.9, 16.6]ng/ml) in the PLP group (P= &lt;0.001). There were no significant correlations between plasma AA (T0 or T60) and any other measured metabolic marker. Single timepoints and retrospective analysis were the main limitations.</div></div><div><h3>Conclusion</h3><div>Plasma AA concentration is not a useful marker for ID or predicting laminitis development in ponies.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"153 ","pages":"Article 105660"},"PeriodicalIF":1.6,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144760284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}