Plasma Activin A concentrations are not a useful biomarker for detecting insulin dysregulation and predicting laminitis risk in ponies

IF 1.6 3区农林科学 Q2 VETERINARY SCIENCES

Journal of Equine Veterinary Science Pub Date : 2025-07-29 DOI:10.1016/j.jevs.2025.105660

C.J. McGuire , E.J. Knowles , P.A. Harris , N.J. Menzies-Gow

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引用次数: 0

Abstract

Background

Circulating insulin concentrations are used to quantify risk of laminitis in currently non-laminitic ponies. Basal serum activin A (AA) concentration has previously been positively correlated with serum insulin concentrations 60 minutes (T60) following an oral sugar test (OST) in ponies with equine metabolic syndrome. Therefore, circulating AA might be a useful marker for insulin dysregulation (ID) and laminitis risk.

Objectives

To explore the relationship between circulating AA concentrations at baseline (T0) and T60 in ponies that developed laminitis within six months and non-laminitic ponies.

Methods

Case-control study. Forty-three ponies that developed laminitis (PLP) during a four-year surveillance period were selected from a larger cohort; 43 ponies from the same cohort that remained non-laminitic (NLP) were selected as controls. Plasma AA concentrations were measured using a validated ELISA at T0 and T60 and compared between groups at both time points using a Wilcoxon signed ranks test. Correlations with other previously measured metabolic markers were investigated using Spearman‘s rank correlation coefficients.

Results

There was a significant difference between T0 plasma AA (median 8.8; interquartile range [6.2, 13.1]ng/mL) and T60 (13.1; [8.8, 20.1]ng/mL) within the NLP group (P= <0.001) and between plasma AA at T0 (7.4 [5.5, 12.4]ng/mL) and T60 (12.3 [6.9, 16.6]ng/ml) in the PLP group (P= <0.001). There were no significant correlations between plasma AA (T0 or T60) and any other measured metabolic marker. Single timepoints and retrospective analysis were the main limitations.

Conclusion

Plasma AA concentration is not a useful marker for ID or predicting laminitis development in ponies.

查看原文本刊更多论文

血浆激活素A浓度不是检测胰岛素失调和预测小马板炎风险的有用生物标志物。

背景：循环胰岛素浓度用于量化目前非板层炎小马板层炎的风险。在患有马代谢综合征的小马中，基础血清激活素A （AA）浓度与口服糖试验（OST）后60分钟（T60）的血清胰岛素浓度呈正相关。因此，循环AA可能是胰岛素失调（ID）和板炎风险的有用标记。目的：探讨6个月内发生板层炎的小马和非板层炎小马的基线循环AA浓度（T0）和T60之间的关系。方法：病例-对照研究。在四年的监测期间，从一个更大的队列中选择了43匹患板部炎（PLP）的小马；从同一队列中选择43匹非带状疱疹（NLP）小马作为对照。在T0和T60时使用有效的ELISA测定血浆AA浓度，并在两个时间点使用Wilcoxon符号秩检验进行组间比较。与其他先前测量的代谢标志物的相关性使用秩相关系数进行研究。结果：T0血浆AA(中位8.8；四分位间距[6.2,13.1]ng/mL)和T60 (13.1；[8.8, 20.1]ng/mL) （P=）结论：血浆AA浓度不能作为马体ID或预测蹄板炎发展的有效指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Equine Veterinary Science 农林科学-兽医学

CiteScore

2.70

自引率

7.70%

发文量

249

审稿时长

77 days

期刊介绍： Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.