{"title":"Opinions of trainers of Warmblood, Thoroughbred and Trotter horses in Germany on the pretraining management of yearlings and two-year-old horses – a qualitative analysis","authors":"F. Pilger , C. Aurich , J.E. Aurich","doi":"10.1016/j.jevs.2025.105736","DOIUrl":"10.1016/j.jevs.2025.105736","url":null,"abstract":"<div><h3>Background</h3><div>The early career start of racehorses, the pretraining of two-year-old Warmblood stallions for licensing, and individual housing of young horses increasingly raise animal welfare concerns.</div></div><div><h3>Aims/objectives</h3><div>Assess pretraining practices and the opinion of trainers on housing of young horses, minimum age to commence training and pretraining injuries.</div></div><div><h3>Methods</h3><div>Qualitative interviews with trainers of Warmblood stallions and Thoroughbred and Trotter racehorses (n = 10 each).</div></div><div><h3>Results</h3><div>Pretraining of Thoroughbreds and Trotters began at 18 months, whereas licensing preparation of Warmblood stallions began at 29-30 months. For horses not achieving the expected results, procedures for Warmblood stallions differed from racehorses. Warmblood stallions not meeting expectations were often castrated and trained as riding horses, whereas Thoroughbreds and Trotters remained in training, but intensity was reduced. Warmblood trainers did not report injuries requiring pretraining interruption. Thoroughbred and Trotter trainers suggested tendon damage as an occasional problem. Group housing was not utilized because of the risk of injury due to agonistic behavior, especially among stallions, the difficulty of individual feeding and disruption in a group whenever horses were removed and returned. Straw bedding was used in 28 out of 30 yards and horses were fed 8-10 kg of hay/day. Except for three yards, horses were provided contact with neighbors through bars in the side walls of the stalls.</div></div><div><h3>Conclusions</h3><div>Trainers largely fulfilled legal horse welfare requirements but horses had limited free movement and social interaction. None of the trainers agreed with the suggestion of a minimum age of 30 months for training.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"156 ","pages":"Article 105736"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Salivary cortisol concentration in western performance horses and riders: Associations with performance","authors":"M.V. Pennington , J.L. Zoller , S.H. White-Springer , J.F. Harlin , C.J. Huseman , J.L. Leatherwood , C.L. O’Reilly","doi":"10.1016/j.jevs.2025.105737","DOIUrl":"10.1016/j.jevs.2025.105737","url":null,"abstract":"<div><h3>Background</h3><div>Competition stress is a common factor influencing the success of performing athletes. However, few studies have quantified the physiological stress responses in horses and riders surrounding an equestrian event.</div></div><div><h3>Aims/objectives</h3><div>The objectives of the current study were to determine salivary cortisol concentrations (SCC) as an indicator of stress in fifteen horse and rider pairs competing in western stock horse events and to investigate the relationship between SCC and performance. Methods: Subjects included male and female Quarter horses aged 3-15 years and equestrians aged 18-22 years. Salivary samples were taken at rest, during practice, during four stock horse classes (pleasure, trail, reining, and working cow horse) in a Stock Horse of Texas sanctioned event, and at a maximal exercise test for riders only. Saliva samples were collected 20 min pre- and 20 min post-test.</div></div><div><h3>Results</h3><div>Rider mean SCC following stock horse pleasure (6.42±1.73 nmol/L) was lower (<em>p</em> < 0.05) than reining (13.53±1.87 nmol/L), cow work (12.85±1.60 nmol/L), and maximal exercise tests (10.05±1.77 nmol/L). There was no significant impact of event on horse SCC (<em>p</em> > 0.05). Novice riders had a greater SCC (10.26+4.73 nmol/L) than non-pro riders (8.92+3.74 nmol/L) post cow work. Rider pre-competition SCC was inversely related to overall placing (<em>p</em> = 0.016, r<sup>2</sup>=0.397) and scores in the reining class (<em>p</em> = 0.044, r<sup>2</sup>=0.239). Horse SCC before reining was also inversely related to score (<em>p</em> = 0.034, r<sup>2</sup>=0.348).</div></div><div><h3>Conclusion</h3><div>An increased rider SCC before competition, negatively impacted the outcome of horse and rider performance; indicating that competition stress may impact equestrian performance in western stock horse events.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"156 ","pages":"Article 105737"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145582259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Horses show limited owner bias in reunion and odor tests: a pilot study","authors":"Ellinor Rönnow, Lina S.V. Roth","doi":"10.1016/j.jevs.2025.105755","DOIUrl":"10.1016/j.jevs.2025.105755","url":null,"abstract":"<div><div>Compared to other companion animals, our understanding of the human-horse relationship is limited, particularly from the horse’s perspective. This pilot study examined whether horses (<em>N</em> = 30) show a bias towards their owner in a reunion in the presence of both the owner and a stranger. Afterwards, the horses were also presented with the odor of the owner and a stranger. Both tests were video recorded for behavioral analyzation. No significant owner-directed bias was observed at the group level in either the reunion test or the odor test. Nonetheless, in the reunion test, owner bias in physical contact - though limited in number - was positively associated with the length of the horse-owner relationships. There were no effects of sex (mares and geldings) or types of horses (Icelandic horses, pony-sized breeds, and larger horse breeds), or age. In the odor test, older horses revealed a stronger owner bias than younger horses, but no significant effects were found for sex, horse type, or relationship length. Hence, we did not find evidence of a conclusive owner bias in our tests. Therefore, we speculate that horses may form a more generalized relationship with humans, based on their previous individual experiences with humans.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"156 ","pages":"Article 105755"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145781439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect on equine sperm of post-thaw glycerol dilution using two different semen extenders","authors":"J.C. Otero , D.M. Neild , A.A. Ferrante , M.C. Gambarotta , M.L. Caldevilla","doi":"10.1016/j.jevs.2025.105747","DOIUrl":"10.1016/j.jevs.2025.105747","url":null,"abstract":"<div><h3>Background</h3><div>Glycerol, a penetrating cryoprotectant, is most employed for deep freezing spermatozoa. However, it can induce toxic, chemical, and osmotic effects, altering the lipid structure of the sperm membrane. Rapid glycerol addition has been demonstrated to have fewer toxic effects than its removal.</div></div><div><h3>Aims/objectives</h3><div>To minimize both the toxic and osmotic effects of glycerol on equine sperm through post-thaw dilution using two different extenders.</div></div><div><h3>Methods</h3><div>Samples of equine semen frozen with 5% glycerol from nine stallions were thawed, re-diluted 1:2, and incubated for 30 minutes at 22°C in Tyrodes (Tyr) and a commercial equine extender (CE). Kinematic parameters were assessed using Computer-Assisted Semen Analysis, plasma membrane integrity and acrosome status with the fluorescein isothiocyanate-peanut agglutinin-propidium iodide stain, lipid peroxidation with the BODIPY581/591 stain, and DNA fragmentation with the Halo test.</div></div><div><h3>Results</h3><div>No significant differences (P>0.05) in total (TM) and progressive (PM) motility were observed between the control (TM 43.8 ± 20.9%; PM 30.6 ± 15.2%) and the tested extenders (TM Tyr 41 ± 22%, CE 40.4 ± 19.2% and PM Tyr 27.5 ± 15.6%, CE 29.7 ± 14.4%). DNA fragmentation and lipid peroxidation also showed no significant differences (P>0.05) between treatments. However, significantly higher percentages (P<0.05) of sperm with intact plasma membranes and acrosomes were recorded in CE (39.4 ± 12.3%) compared to the control (33.9 ± 10.5%) and Tyr (33.5 ± 12.1%).</div></div><div><h3>Conclusion</h3><div>post-thaw dilution did not yield beneficial effects on equine sperm parameters. However, dilution in CE potentially reduces the proportion of sperm with reacted acrosomes.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"156 ","pages":"Article 105747"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145723891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M.F. Posada-Guzmán , A.E. Jiménez-Rocha , J.F. Sánchez-Bermúdez , J.J. Romero-Zúñiga , G. Dolz
{"title":"Babesia caballi and Theileria equi in ticks from horses in four Indigenous communities of Costa Rica","authors":"M.F. Posada-Guzmán , A.E. Jiménez-Rocha , J.F. Sánchez-Bermúdez , J.J. Romero-Zúñiga , G. Dolz","doi":"10.1016/j.jevs.2025.105750","DOIUrl":"10.1016/j.jevs.2025.105750","url":null,"abstract":"<div><h3>Background</h3><div>Equine piroplasmosis, caused by <em>Babesia caballi</em> and <em>Theileria equi</em>, is endemic in Costa Rica. However, the presence of these hemoparasites in tick vectors has not been documented in the country.</div></div><div><h3>Aims/objectives</h3><div>To identify tick species infesting horses in four Indigenous communities and to determine the presence of <em>B. caballi</em> and <em>T. equi</em> DNA in ticks from piroplasmosis-positive horses.</div></div><div><h3>Methods</h3><div>Ticks (<em>n</em> = 1063) were collected from 129 horses in four Indigenous communities. Species identification and sex classification were performed. From 76 PCR-positive horses, 203 ticks were analyzed for <em>B. caballi</em> and <em>T. equi</em> using nested PCR. Two positive samples were sequenced and compared to GenBank entries<em>.</em></div></div><div><h3>Results</h3><div><em>Dermacentor nitens</em> was the predominant tick (90.3 %), followed by <em>Amblyomma mixtum</em> (9.7 %). <em>Theileria equi</em> was detected in ticks from 55.3 % of positive horses; <em>B. caballi</em> was only found in coinfection with <em>T. equi</em>. Sequences showed 99 % similarity with GenBank references.</div></div><div><h3>Conclusion</h3><div>This is the first report of <em>B. caballi</em> and <em>T. equi</em> DNA detection in ticks from Costa Rica. <em>Dermacentor nitens</em> and <em>A. mixtum</em> are implicated in potential transmission. Surveillance and tick control are important in Indigenous communities to prevent the spread of pathogens. The study was based on tick samples collected in 2011; thus, results reflect the situation at that time. Updated surveillance and vector competence studies are needed to support equine health and One-Health efforts in Indigenous regions of Costa Rica.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"156 ","pages":"Article 105750"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145695997","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
B. Pepper , E. North , J. Culwell , M.C. Nicodemus , C. Cavinder , K. Harvey , T. Williams
{"title":"Pedigree tracing to determine the origin of the golden coat phenotype within the golden American saddlebred horse","authors":"B. Pepper , E. North , J. Culwell , M.C. Nicodemus , C. Cavinder , K. Harvey , T. Williams","doi":"10.1016/j.jevs.2025.105752","DOIUrl":"10.1016/j.jevs.2025.105752","url":null,"abstract":"<div><h3>Background</h3><div>Due to the incorporation of the Golden American Saddlebred Horse Association (GASHA) into the American Saddlebred Horse and Breeders Association (ASHBA), breeding for coat color has become more challenging for horse breeders. However, with this merger, pedigree tracing can be of value in pinpointing foundation bloodlines within the GASHA that influence production of the golden coat phenotype.</div></div><div><h3>Objectives</h3><div>The study objective was to document the historical origins of the GASHA through pedigree tracing to determine the influence of dilution alleles that produce the golden coat phenotype.</div></div><div><h3>Methods</h3><div>A sample of 550 horses was obtained from the GASHA Official Directory. Using the All-Breed Database, sampled horses were examined for pedigrees suitable for color inheritance analysis with 507 being found to have extended pedigrees with coat color documentation that were traced to record lineage and associated coat color assignments.</div></div><div><h3>Results</h3><div>Pedigrees traced determined 43 % carried the <em>champagne</em> dilution allele, 36.1 % carried the <em>crème</em> dilution allele, and 20 % had non-informative pedigrees that either traced back to unknown ancestors or were found to have incorrect ancestry. Another 0.9 % were determined to be obligate carriers for the <em>crème</em> dilution allele due to conflicting color designation. All pedigrees traced back to six foundation mares with the majority tracing back to two of these mares, Maud which carried the <em>champagne</em> dilution allele and Allens Queen which carried the <em>crème</em> dilution allele.</div></div><div><h3>Conclusion</h3><div>Through pedigree tracing the GASHA origins were documented, unveiling genetic contributions for today's GASHA golden coat phenotype that can facilitate selective breeding practices.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"156 ","pages":"Article 105752"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145742890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Deep learning approach for classifying grazing behavior in yearling horses using triaxial accelerometer data: A pilot study","authors":"Uta Kamiya , Kasumi Kakiuchi , Kensuke Kawamura , Koichiro Ueda , Masahito Kawai , Akira Matsui , Natsuko Negishi","doi":"10.1016/j.jevs.2025.105706","DOIUrl":"10.1016/j.jevs.2025.105706","url":null,"abstract":"<div><h3>Background</h3><div>Accurate monitoring of grazing behavior in horses is essential for pasture management and welfare evaluation; however, conventional observation methods are labor-intensive and lack temporal resolution.</div></div><div><h3>Aims/objectives</h3><div>This pilot study aimed to develop and validate a deep learning model using jaw-mounted accelerometer data to classify grazing and non-grazing behaviors in yearling horses under various pasture conditions.</div></div><div><h3>Methods</h3><div>Four yearling Thoroughbred horses were equipped with triaxle accelerometers mounted under their jaws. Data were recorded at 10 Hz (100 ms) during a 19 h free-grazing period in a 4.0 ha paddock. A total of 230,286 data points were annotated as grazing (G) or non-grazing (NG) using synchronized video observation. Three deep learning models—one-dimensional convolutional neural network (CNN), long short-term memory (LSTM), and combined CNN+LSTM—were trained and evaluated under varying sampling rates (100–10,000 ms) and time windows (5–60 s). Model performance was assessed using accuracy, F1 score, precision, recall, and area under the curve (AUC).</div></div><div><h3>Results</h3><div>The CNN+LSTM model demonstrated the highest classification performance with a test accuracy of 98.0 % and an AUC of 1.00. F1 scores were 0.99 for G and 0.97 for NG behavior. Across the full observational period, the proportion of grazing behavior was 58.3 % (±2.1 %). Spatial analysis revealed that grazing was concentrated along paddock peripheries, whereas non-grazing was more frequent in central zones.</div></div><div><h3>Conclusion</h3><div>A deep learning framework that combines CNN and LSTM can accurately classify grazing behavior in horses using jaw-mounted accelerometers. This non-invasive, high-resolution method offers a promising tool for automated behavioral monitoring in pasture-based systems.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"155 ","pages":"Article 105706"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145225114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Claudia Giannone , Elie Atallah , Emanuela Dalla Costa , Eleonora Benetti , Enrica Santolini , Patrizia Tassinari , Marco Bovo
{"title":"Impact of the technology to monitor horse behaviour and health: a scoping review","authors":"Claudia Giannone , Elie Atallah , Emanuela Dalla Costa , Eleonora Benetti , Enrica Santolini , Patrizia Tassinari , Marco Bovo","doi":"10.1016/j.jevs.2025.105734","DOIUrl":"10.1016/j.jevs.2025.105734","url":null,"abstract":"<div><div>Technology for monitoring behaviour and health of horses has evolved significantly, presenting new opportunities and challenges in equine management and healthcare. This scoping review consolidates recent findings on wearable sensors and non-contact technologies, discussing their benefits and limitations. Among these advancements, heart rate monitors stand out as a key point in equine monitoring. By tracking heart rate variability, devices help monitor cardiac autonomic regulation, optimise training regimens and allow early detection of cardiac issues, although accuracy can be compromised by inadequate placement or signal interference. Beyond heart rate monitors, wearable biometric sensors and smart stable systems are revolutionising equine care. Biometric sensors track vital signs, temperature, and activity levels, offering continuous monitoring of health data. However, challenges remain regarding data accuracy, device durability, and integration with existing systems. Non-contact technology like computer vision provides a non-invasive method of analysing images and videos to detect horses, recognise specific features and track movement over time. This technology helps monitor behavioural patterns, social interactions, and overall activity. In conclusion, the application of wearable and remote monitoring technologies has shown specific benefits in supporting equine welfare. This review presents an overview of current sensors that support daily horse management and promote animal health through the detection of behavioural changes. These metrics could prevent the development of more serious issues. However, addressing limitations such as accuracy, reliability, and privacy concerns is essential. Scientific validation is necessary for guaranteeing the effectiveness of these systems and to maximise their potential to improve equine health and performance.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"155 ","pages":"Article 105734"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145534241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brendo Junior Pereira Farias , Dermeval Araújo Furtado , José Wallace Barbosa do Nascimento , José Pinheiro Lopes Neto , Fabiana Terezinha Leal de Morais , Thiago Lira Souza Santos , Alexandre Sales Vasconcelos , Rafael Costa Silva , José Ilton Pereira Alves , Concepta Mcmanus , Robson Mateus Freitas Silveira , Neila Lidiany Ribeiro
{"title":"Proof of concept of a wearable IoT-based system for monitoring respiratory rate and surface temperature in horses","authors":"Brendo Junior Pereira Farias , Dermeval Araújo Furtado , José Wallace Barbosa do Nascimento , José Pinheiro Lopes Neto , Fabiana Terezinha Leal de Morais , Thiago Lira Souza Santos , Alexandre Sales Vasconcelos , Rafael Costa Silva , José Ilton Pereira Alves , Concepta Mcmanus , Robson Mateus Freitas Silveira , Neila Lidiany Ribeiro","doi":"10.1016/j.jevs.2025.105729","DOIUrl":"10.1016/j.jevs.2025.105729","url":null,"abstract":"<div><div>This study presents the development and proof of concept of a wearable monitoring system designed to measure respiratory rate (RR) and coat surface temperature (CST) in horses. The device integrates an embedded system based on an ESP-32 microcontroller equipped with sensors for RR (strain gauge) and CST (DS18B20) Data are transmitted in real time via Wi-Fi and stored locally on SD cards. The system was validated against conventional manual methods under field conditions. Agreement between methods was evaluated using Intraclass Correlation Coefficients (ICC) and Bland-Altman plots. Results showed excellent reproducibility for RR (ICC = 0.85) and very good reproducibility for CST (ICC = 0.67), with no significant differences detected by paired t-tests (<em>p</em> > 0.05). The Bland-Altman analysis indicated low bias and narrow limits of agreement for both variables. This proof-of-concept demonstrates that the wearable system provides reliable, accurate, and non-invasive measurements of key physiological and environmental parameters in horses.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"155 ","pages":"Article 105729"},"PeriodicalIF":1.6,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145488852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}