子宫内膜异位症与垂体间质功能障碍(PPID)关系的探讨

IF 1.3 3区 农林科学 Q2 VETERINARY SCIENCES
I. Hamner, J. Howard, R.A. Crook, G. Unger, S.J. Coleman, P.M. McCue, C.E. Fedorka
{"title":"子宫内膜异位症与垂体间质功能障碍(PPID)关系的探讨","authors":"I. Hamner,&nbsp;J. Howard,&nbsp;R.A. Crook,&nbsp;G. Unger,&nbsp;S.J. Coleman,&nbsp;P.M. McCue,&nbsp;C.E. Fedorka","doi":"10.1016/j.jevs.2025.105504","DOIUrl":null,"url":null,"abstract":"<div><div>Pituitary pars intermedia dysfunction (PPID) is an endocrine disorder associated with elevated adrenocorticotropin hormone (ACTH). Animals suffering from this disease have been found to have elevated systemic inflammation. It is unknown if this inflammation is noted within the reproductive tract, or if this inflammation is associated with fibrosis. Fibrosis of the endometrium, deemed endometriosis, is often noted in aged mares and is associated with subfertility. Therefore, the objective of this study is to evaluate endometriosis of the PPID mare. We hypothesize that elevated ACTH and its associated chronic inflammation will coincide with an increase in endometrial fibrosis. To evaluate this, 11 mares were screened for PPID in late summer using a thyrotropin-releasing hormone (TRH) stimulation test. In brief, ACTH concentrations were measured before and 1 h after stimulation with 1.0 mg TRH. Of these, 7 mares were found to be PPID positive (n = 7; ACTH &gt;110 pg/mL), and 4 were found to be PPID negative (n = 4; ACTH &lt;30 pg/mL). When mares were in diestrus (presence of a corpus luteum; uterine tone; P4 &gt;4 ng/mL), 2 endometrial biopsies were then collected. One biopsy was processed for qPCR analysis of select inflammatory cytokines and the other was paraffin embedded for histology. Statistics were performed on SAS 9.4, and data were assessed for normality and equal variances utilizing a Bartlett's and Shapiro–Wilk test. Next, the impact of PPID on expression of transcripts associated with fibrosis (MMP1, MMP2, MMP9, TIMP-2, and TNF) were evaluated using an unequal variances <em>t</em>-test. Correlation between ACTH and expression of fibrosis markers was assessed using a Pearson correlation. Significance was set at <em>P</em> &lt; 0.05. PPID was not found to affect the expression of any transcript evaluated, and this included MMP2 (<em>P</em> = 0.56), MMP9 (<em>P</em> = 0.91), TIMP-2 (<em>P</em> = 0.19), and TNF (<em>P</em> = 0.35). There was also no significant correlation between levels of ACTH and expression of fibrotic markers, including MMP2 (<em>P</em> = 0.61; R<sup>2</sup> = 0.04), MMP9 (<em>P</em> = 0.66; R<sup>2</sup> = 0.03), TIMP-2 (<em>P</em> = 0.97; R<sup>2</sup> &lt; 0.01), or TNF (<em>P</em> = 0.53; R<sup>2</sup> = 0.05). Additionally, there was no significant difference when assessing fibrosis based on histology, as fibrosis was noted in 4 of 7 PPID animals and 2 of 4 control animals (<em>P</em> = 0.82). In conclusion, PPID does not appear to have a correlation with endometriosis.</div></div>","PeriodicalId":15798,"journal":{"name":"Journal of Equine Veterinary Science","volume":"148 ","pages":"Article 105504"},"PeriodicalIF":1.3000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Investigation into the association between endometriosis and pituitary pars intermedia dysfunction (PPID)\",\"authors\":\"I. Hamner,&nbsp;J. Howard,&nbsp;R.A. Crook,&nbsp;G. Unger,&nbsp;S.J. Coleman,&nbsp;P.M. McCue,&nbsp;C.E. Fedorka\",\"doi\":\"10.1016/j.jevs.2025.105504\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Pituitary pars intermedia dysfunction (PPID) is an endocrine disorder associated with elevated adrenocorticotropin hormone (ACTH). Animals suffering from this disease have been found to have elevated systemic inflammation. It is unknown if this inflammation is noted within the reproductive tract, or if this inflammation is associated with fibrosis. Fibrosis of the endometrium, deemed endometriosis, is often noted in aged mares and is associated with subfertility. Therefore, the objective of this study is to evaluate endometriosis of the PPID mare. We hypothesize that elevated ACTH and its associated chronic inflammation will coincide with an increase in endometrial fibrosis. To evaluate this, 11 mares were screened for PPID in late summer using a thyrotropin-releasing hormone (TRH) stimulation test. In brief, ACTH concentrations were measured before and 1 h after stimulation with 1.0 mg TRH. Of these, 7 mares were found to be PPID positive (n = 7; ACTH &gt;110 pg/mL), and 4 were found to be PPID negative (n = 4; ACTH &lt;30 pg/mL). When mares were in diestrus (presence of a corpus luteum; uterine tone; P4 &gt;4 ng/mL), 2 endometrial biopsies were then collected. One biopsy was processed for qPCR analysis of select inflammatory cytokines and the other was paraffin embedded for histology. Statistics were performed on SAS 9.4, and data were assessed for normality and equal variances utilizing a Bartlett's and Shapiro–Wilk test. Next, the impact of PPID on expression of transcripts associated with fibrosis (MMP1, MMP2, MMP9, TIMP-2, and TNF) were evaluated using an unequal variances <em>t</em>-test. Correlation between ACTH and expression of fibrosis markers was assessed using a Pearson correlation. Significance was set at <em>P</em> &lt; 0.05. PPID was not found to affect the expression of any transcript evaluated, and this included MMP2 (<em>P</em> = 0.56), MMP9 (<em>P</em> = 0.91), TIMP-2 (<em>P</em> = 0.19), and TNF (<em>P</em> = 0.35). There was also no significant correlation between levels of ACTH and expression of fibrotic markers, including MMP2 (<em>P</em> = 0.61; R<sup>2</sup> = 0.04), MMP9 (<em>P</em> = 0.66; R<sup>2</sup> = 0.03), TIMP-2 (<em>P</em> = 0.97; R<sup>2</sup> &lt; 0.01), or TNF (<em>P</em> = 0.53; R<sup>2</sup> = 0.05). Additionally, there was no significant difference when assessing fibrosis based on histology, as fibrosis was noted in 4 of 7 PPID animals and 2 of 4 control animals (<em>P</em> = 0.82). In conclusion, PPID does not appear to have a correlation with endometriosis.</div></div>\",\"PeriodicalId\":15798,\"journal\":{\"name\":\"Journal of Equine Veterinary Science\",\"volume\":\"148 \",\"pages\":\"Article 105504\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Equine Veterinary Science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0737080625001625\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Equine Veterinary Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0737080625001625","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

摘要

垂体部中间功能障碍(PPID)是一种与促肾上腺皮质激素(ACTH)升高相关的内分泌疾病。患有这种疾病的动物被发现全身炎症升高。尚不清楚这种炎症是否发生在生殖道内,或者这种炎症是否与纤维化有关。子宫内膜纤维化,被认为是子宫内膜异位症,经常在老年母马中发现,并与生育能力低下有关。因此,本研究的目的是评估PPID母马的子宫内膜异位症。我们假设ACTH升高及其相关的慢性炎症将与子宫内膜纤维化的增加同时发生。为了评估这一点,11匹母马在夏末使用促甲状腺激素释放激素(TRH)刺激试验筛选PPID。简而言之,在1.0 mg TRH刺激前和刺激后1 h测量ACTH浓度。其中,7匹母马PPID阳性(n = 7;ACTH >110 pg/mL), PPID阴性4例(n = 4;ACTH 30 pg/mL)。当母马处于发情期(黄体存在);子宫基调;P4 >4 ng/mL),然后收集2个子宫内膜活检。一个活检组织进行qPCR分析选择的炎症细胞因子,另一个石蜡包埋进行组织学检查。采用SAS 9.4进行统计,并利用Bartlett's和Shapiro-Wilk检验评估数据的正态性和等方差。接下来,使用不等方差t检验评估PPID对纤维化相关转录物(MMP1、MMP2、MMP9、TIMP-2和TNF)表达的影响。使用Pearson相关性评估ACTH与纤维化标志物表达的相关性。P <;0.05. 未发现PPID影响任何转录物的表达,包括MMP2 (P = 0.56)、MMP9 (P = 0.91)、TIMP-2 (P = 0.19)和TNF (P = 0.35)。ACTH水平与纤维化标志物MMP2表达也无显著相关性(P = 0.61;R2 = 0.04),mmp9 (p = 0.66;R2 = 0.03),timp-2 (p = 0.97;R2 & lt;0.01)或TNF (P = 0.53;R2 = 0.05)。此外,在基于组织学评估纤维化时,没有显着差异,因为7只PPID动物中有4只和4只对照动物中有2只出现了纤维化(P = 0.82)。总之,PPID似乎与子宫内膜异位症无关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation into the association between endometriosis and pituitary pars intermedia dysfunction (PPID)
Pituitary pars intermedia dysfunction (PPID) is an endocrine disorder associated with elevated adrenocorticotropin hormone (ACTH). Animals suffering from this disease have been found to have elevated systemic inflammation. It is unknown if this inflammation is noted within the reproductive tract, or if this inflammation is associated with fibrosis. Fibrosis of the endometrium, deemed endometriosis, is often noted in aged mares and is associated with subfertility. Therefore, the objective of this study is to evaluate endometriosis of the PPID mare. We hypothesize that elevated ACTH and its associated chronic inflammation will coincide with an increase in endometrial fibrosis. To evaluate this, 11 mares were screened for PPID in late summer using a thyrotropin-releasing hormone (TRH) stimulation test. In brief, ACTH concentrations were measured before and 1 h after stimulation with 1.0 mg TRH. Of these, 7 mares were found to be PPID positive (n = 7; ACTH >110 pg/mL), and 4 were found to be PPID negative (n = 4; ACTH <30 pg/mL). When mares were in diestrus (presence of a corpus luteum; uterine tone; P4 >4 ng/mL), 2 endometrial biopsies were then collected. One biopsy was processed for qPCR analysis of select inflammatory cytokines and the other was paraffin embedded for histology. Statistics were performed on SAS 9.4, and data were assessed for normality and equal variances utilizing a Bartlett's and Shapiro–Wilk test. Next, the impact of PPID on expression of transcripts associated with fibrosis (MMP1, MMP2, MMP9, TIMP-2, and TNF) were evaluated using an unequal variances t-test. Correlation between ACTH and expression of fibrosis markers was assessed using a Pearson correlation. Significance was set at P < 0.05. PPID was not found to affect the expression of any transcript evaluated, and this included MMP2 (P = 0.56), MMP9 (P = 0.91), TIMP-2 (P = 0.19), and TNF (P = 0.35). There was also no significant correlation between levels of ACTH and expression of fibrotic markers, including MMP2 (P = 0.61; R2 = 0.04), MMP9 (P = 0.66; R2 = 0.03), TIMP-2 (P = 0.97; R2 < 0.01), or TNF (P = 0.53; R2 = 0.05). Additionally, there was no significant difference when assessing fibrosis based on histology, as fibrosis was noted in 4 of 7 PPID animals and 2 of 4 control animals (P = 0.82). In conclusion, PPID does not appear to have a correlation with endometriosis.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Journal of Equine Veterinary Science
Journal of Equine Veterinary Science 农林科学-兽医学
CiteScore
2.70
自引率
7.70%
发文量
249
审稿时长
77 days
期刊介绍: Journal of Equine Veterinary Science (JEVS) is an international publication designed for the practicing equine veterinarian, equine researcher, and other equine health care specialist. Published monthly, each issue of JEVS includes original research, reviews, case reports, short communications, and clinical techniques from leaders in the equine veterinary field, covering such topics as laminitis, reproduction, infectious disease, parasitology, behavior, podology, internal medicine, surgery and nutrition.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信