Journal of Experimental Biology最新文献_第2页

Hummingbirds excel at maneuvering and flying through tight spaces. 蜂鸟擅长机动和在狭小空间中飞行。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-13 DOI: 10.1242/jeb.250269

Zixuan M Zhang, Nicolai Konow, Andrew A Biewener

{"title":"Hummingbirds excel at maneuvering and flying through tight spaces.","authors":"Zixuan M Zhang, Nicolai Konow, Andrew A Biewener","doi":"10.1242/jeb.250269","DOIUrl":"https://doi.org/10.1242/jeb.250269","url":null,"abstract":"Flying animals (birds, bats, and insects) often negotiate cluttered environments, sometimes involving the need to safely transit through tight spaces between obstacles. Considering their remarkable flight capabilities, hummingbirds are suitable for gaining a better understanding of how flying animals transit tight passages. We studied wild ruby-throated hummingbirds (Archilochus colubris) transiting a chain-link fence with openings smaller than their wingspan to reach a feeder. We identified two distinct flight strategies, involving swept-wing versus sideways styles. Swept-wing transits were characterized by interrupted flapping and a retracted posture with a ballistic body trajectory and were faster than sideways transits, which involved, continuous flapping with a reduced wingbeat amplitude, higher wingbeat frequencies and more uniform body pitch and yaw compared to the body movements dynamics used in swept-wing transits. Our novel data for free-flying hummingbirds voluntarily negotiating narrow openings to access food reinforce earlier findings of captive hummingbirds, demonstrating that some fliers can modulate their wing kinematics and body rotations sufficiently to safely navigate openings smaller than their wingspan, a finding that may reflect a more general strategy across fliers. This study contributes both to understanding biological flight dynamics in cluttered environments and potentially to informing the design of autonomous aerial vehicles.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene expression correlates and mechanistic insights into electric organ discharge duration change in mormyrid electric fish. 电鱼电器官放电持续时间变化的基因表达相关性及机制研究。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-12 DOI: 10.1242/jeb.249548

Mauricio Losilla, Jason R Gallant

{"title":"Gene expression correlates and mechanistic insights into electric organ discharge duration change in mormyrid electric fish.","authors":"Mauricio Losilla, Jason R Gallant","doi":"10.1242/jeb.249548","DOIUrl":"https://doi.org/10.1242/jeb.249548","url":null,"abstract":"Electric organ discharge (EOD) duration in African weakly electric fish (Mormyridae) is the most variable waveform component between species and the basis for distinguishing species-specific signals. EOD duration is thought to be influenced by morphological and physiological features of electrocytes (the cells that comprise the electric organ), but the mechanistic details are poorly understood. It has long been known that EOD duration is modulated by androgen hormones, affording an opportunity to identify gene expression correlates of EOD duration differences. We induced EOD elongation in the mormyrid Brienomyrus brachyistius by administering 17α-methyltestosterone (17αMT) to three treatment groups: control (no 17αMT exposure), T1day and T8day (samples taken one and eight days after a single exposure to 17αMT, respectively). We then performed RNAseq, differential gene expression, and functional enrichment analysis to detect gene expression changes during EOD duration change. Our analyses indicate 44 genes whose expression changed in tandem with EOD elongation and include genes responsible for actin filaments and microtubules, extracellular matrix organization, and membrane lipid metabolism. Additionally, we found expression changes in one Na+ channel beta subunit, and five K+ voltage-gated channels. Together, these genes point toward specific cellular processes that contribute to morphological and physiological changes that contribute to EOD duration changes.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic stress and the development of the depression-like neuroendocrine profile in Atlantic salmon. 大西洋鲑鱼慢性应激与抑郁样神经内分泌的发展。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-12 DOI: 10.1242/jeb.249862

Marco A Vindas, Ole Folkedal, Johan Aerts, Øyvind Øverli, Jonatan Nilsson, Ida B Johansen, Tore S Kristiansen, Erik Höglund

{"title":"Chronic stress and the development of the depression-like neuroendocrine profile in Atlantic salmon.","authors":"Marco A Vindas, Ole Folkedal, Johan Aerts, Øyvind Øverli, Jonatan Nilsson, Ida B Johansen, Tore S Kristiansen, Erik Höglund","doi":"10.1242/jeb.249862","DOIUrl":"https://doi.org/10.1242/jeb.249862","url":null,"abstract":"A subpopulation of aquaculture salmon are characterized by abnormal swimming behavior, growth stunting, and anorexia, as well as chronically elevated cortisol and brain serotonergic levels. This profile is associated with a depression-like state (DLS) and these fish are unable to respond to further stressors. Whereas the underlying causes behind this phenomenon remain elusive, the physiological profile strongly suggests that chronic stress plays a significant role in this phenomenon. We subjected Atlantic salmon to a chronic stress regime consisting of incremental increases in environmental CO2 concentrations during the freshwater phase for 68 days. Plasma corticosteroids, brain stem, hypothalamic and telencephalic serotonin concentrations and telencephalic whole transcriptome expression was then assessed under basal and acute stress conditions. We found that CO2 fish were characterized by a long-term increase in cortisol, cortisol+cortisone and serotonin (5-HT) signaling in the brain stem. Furthermore, in response to an acute confinement stressor, the CO2 fish increased their levels of cortisol, cortisol+cortisone, and decreased their cortisone/cortisol ratio. But unlike the control fish, they were unable to also respond to confinement by increased 5-HT signaling in the brain stem. In terms of their transcriptional response, post stress gene regulation in CO2 fish was the opposite of that observed in control fish. We believe this profile is an example of allostatic overload, characterized by the inability to cope with stress. This profile is associated with DLS, suggesting that chronic stress may be an important factor leading up to the development of the DLS phenotype in salmon.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143988138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bumblebees locate goals in 3D with absolute height estimation from ventral optic flow. 大黄蜂利用腹侧光流估计的绝对高度来定位三维目标。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-12 DOI: 10.1242/jeb.249763

Annkathrin Sonntag, Martin Egelhaaf, Olivier J N Bertrand, Mathieu Lihoreau

{"title":"Bumblebees locate goals in 3D with absolute height estimation from ventral optic flow.","authors":"Annkathrin Sonntag, Martin Egelhaaf, Olivier J N Bertrand, Mathieu Lihoreau","doi":"10.1242/jeb.249763","DOIUrl":"https://doi.org/10.1242/jeb.249763","url":null,"abstract":"When foraging, flying animals like bees are often required to change their flight altitude from close to the ground to above the height of vegetation to reach their nest or a food source. While the mechanisms of navigating towards a goal in two dimensions are well investigated, the explicit use of height as a source for navigation in three dimensions remains mostly unknown. Our study aims to unravel which strategies bumblebees use for height estimation and whether they rely on global or local cues. We expanded a 2D goal localization paradigm, where a goal location is indicated by cylindrical landmarks, to the third dimension by using spherical landmarks to indicate a feeder's position in 3D and examined the search pattern of bumblebees. Additionally, we assessed the ability of bees to estimate the height of a feeder based on local landmarks and global references such as the ground. The search distribution for a feeder's position in 3D was less spatially concentrated compared to 2D. Assessing the bees' height estimation ability, we found bees could estimate a feeder's height using the ground as a reference. However, the feeder needed to be sufficiently close to the ground for the bees to choose correctly. When bumblebees are faced with the challenge of foraging in a 3D environment where the height of a food source and landmark cues are important, they demonstrate the ability to learn and return to a specific flower height. This suggests bumblebees rely on ventral optic flow for goal height estimation.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How consistent is 'the dynamic gut'?: Complex physiological responses to dietary fiber and protein across three rodent species. “动态肠道”的一致性如何？三种啮齿类动物对膳食纤维和蛋白质的复杂生理反应。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-12 DOI: 10.1242/jeb.249797

Kevin D Kohl, Nick Barts, Karen Peralta Martínez, Anna Lackey, Emily Lyons, Matthew J Maier, Maya Maurer, Domenique Tripoli, Tate Yawitz, Rodolfo Martínez-Mota, Bret Pasch, M Denise Dearing, Brian K Trevelline

{"title":"How consistent is 'the dynamic gut'?: Complex physiological responses to dietary fiber and protein across three rodent species.","authors":"Kevin D Kohl, Nick Barts, Karen Peralta Martínez, Anna Lackey, Emily Lyons, Matthew J Maier, Maya Maurer, Domenique Tripoli, Tate Yawitz, Rodolfo Martínez-Mota, Bret Pasch, M Denise Dearing, Brian K Trevelline","doi":"10.1242/jeb.249797","DOIUrl":"https://doi.org/10.1242/jeb.249797","url":null,"abstract":"To efficiently digest food resources that may vary spatially and temporally, animals maintain physiological flexibility across levels of organization. For example, in response to dietary shifts, animals may exhibit changes in the expression of digestive enzymes, sizes of digestive organs, or the structure of their gut microbiome. A 'Grand Challenge' in comparative physiology is to understand how components of flexibility across organizational levels may scale to cumulatively determine organismal performance. Here, we conducted feeding trials on three rodent species with disparate feeding strategies: herbivorous montane voles (Microtus montanus), omnivorous white-footed mice (Peromyscus leucopus), and carnivorous grasshopper mice (Onychomys torridus). For each species, four groups of individuals were presented with diets that varied in carbohydrate, fiber, and protein content. After 4-5 weeks, we measured organismal performance in the form of nutrient digestibility (dry matter, nitrogen, fiber). We also measured gut anatomy, organ sizes, and conducted enzyme assays on various tissues to measure activities of carbohydrases and peptidases. We found some shared physiological responses e.g., fiber generally increased gut size across species. However, the specifics of these responses were distinct across species, suggesting different capacity for flexibility. Thus, in the context of digestion, we still lack an understanding of how flexibility across organizational levels may scale to determine whole animal performance.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gut bacterial and fungal communities of three rodent species respond uniquely to dietary fiber and protein manipulation. 三种啮齿类动物的肠道细菌和真菌群落对膳食纤维和蛋白质的操纵有独特的反应。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-12 DOI: 10.1242/jeb.249794

Nick Barts, Brian K Trevelline, M Denise Dearing, Rodolfo Martínez-Mota, Bret Pasch, Kevin D Kohl

{"title":"Gut bacterial and fungal communities of three rodent species respond uniquely to dietary fiber and protein manipulation.","authors":"Nick Barts, Brian K Trevelline, M Denise Dearing, Rodolfo Martínez-Mota, Bret Pasch, Kevin D Kohl","doi":"10.1242/jeb.249794","DOIUrl":"https://doi.org/10.1242/jeb.249794","url":null,"abstract":"Diet and host identity play fundamental roles in digestive physiology and the assembly of gut microbial communities. Research shows that microbial communities are plastic, with abundances of taxa and community interactions exhibiting changes in response to diet. Few studies considering the influence of diet on host and microbial plasticity disentangle the unique roles of specific nutrients, such as protein and fiber. Additionally, in the context of host-microbiome interactions, few studies have explored how host dietary strategies shape the plastic responses of microbial communities within the host digestive tract. To address these current gaps, we fed rodents with distinct dietary strategies (Peromyscus leucopus , Microtus montanus , and Onychomys torridus) diet treatments varying in fiber and protein content. Species varied in the degree of cecum size plasticity, with the carnivore showing no significant changes and the omnivore responding to both fiber and protein manipulation. There were also differences in the diversity indices of bacterial and fungal communities across hosts, and the microbes driving those differences were largely unique across rodent species. Additionally, community network interactions varied across treatments, and hub taxa that play a role in regulating network properties were identified. For example, bacteria in the Eubacterium groups, which are known to aid in fiber fermentation, were identified as hub taxa in all three species, but no group shared the same Eubacterium as a hub taxa. Overall, our data suggests that hosts with unique dietary strategies and their microbiomes respond uniquely to changes in the nutrient composition of their diets.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tapered insect (Acheta domesticus) antennae have rapid damped return with minimal oscillation after perturbation. 锥形昆虫（Acheta domesticus）的触角在扰动后具有最小振荡的快速阻尼返回。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-06 DOI: 10.1242/jeb.249243

Marlo G McCarter, Derek Kellogg, Stanley Sowy, Catherine Loudon

{"title":"Tapered insect (Acheta domesticus) antennae have rapid damped return with minimal oscillation after perturbation.","authors":"Marlo G McCarter, Derek Kellogg, Stanley Sowy, Catherine Loudon","doi":"10.1242/jeb.249243","DOIUrl":"10.1242/jeb.249243","url":null,"abstract":"As tactile sensors, antennae must be flexible and responsive while maintaining shape and control of the structure. We evaluated the geometric and mechanical properties of cricket antennae, which we treat as bending cantilever beams. Flexural rigidity (EI) is the mechanical property that most significantly controls bending behavior. We determined that the flexural rigidity decreases steeply (proximal to distal) by evaluating the quasistatic bent shapes in response to obstacle contact at different points along the antennae. This steep decrease in flexural rigidity causes the antennae to bend readily only near the obstacle contact, in contrast to the curvature of a beam with uniform properties and cross-section (which bends closer to the base). This flexural rigidity gradient in the antennae is consistent with the morphology: a decreasing second moment of area calculated from the measured taper and the diminishing wall (cuticle) thickness. Cricket antennae recovered from a single localized perturbation quickly and with minimal to no oscillation, suggesting behavior close to critical damping (fastest return without oscillations). Bending primarily occurred in the portion of the flagellum near the obstacle contact, reducing the length of the flagellum that participated in the oscillating behavior (natural frequency ∼11 Hz). Forced sinusoidal vibrations generated a resonance frequency of ∼30 Hz with imperceptible movement in the proximal part of the flagellum while the distal part vibrated. The results suggest that tapering of an elongated mechanosensor may facilitate a rapid return to its original shape without oscillation, which is an advantageous attribute that may also inform biomimetic applications.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079660/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seminal vesicle protein caCA12 in Corydoras aeneus inhibits sperm motility for sperm drinking. 凤尾蝶精囊蛋白caCA12对饮精后精子运动的抑制作用。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-15 DOI: 10.1242/jeb.250293

Junki Yoshida, Maho Yamamoto, Junki Kamiya, Akinaga Kondo, Yukihito Sakaguchi, Nanami Morino, Takako Saito, Masanori Kohda, Satoshi Awata, Ban Sato, Kenji Miyado, Natsuko Kawano

{"title":"Seminal vesicle protein caCA12 in Corydoras aeneus inhibits sperm motility for sperm drinking.","authors":"Junki Yoshida, Maho Yamamoto, Junki Kamiya, Akinaga Kondo, Yukihito Sakaguchi, Nanami Morino, Takako Saito, Masanori Kohda, Satoshi Awata, Ban Sato, Kenji Miyado, Natsuko Kawano","doi":"10.1242/jeb.250293","DOIUrl":"https://doi.org/10.1242/jeb.250293","url":null,"abstract":"Seminal vesicle (SV) secretions enhance fertilization by regulating sperm motility and fertilization capacity, and by forming plugs that prevent mating with other males. Although SVs are rare in teleosts, certain species, such as Corydoras spp., do possess them. In Corydoras spp. and other species that exhibit sperm drinking or related behaviors, females attach their mouths to the males' genital pore to ingest semen, a reproductive behavior known as sperm drinking. However, the major proteins and functions of seminal vesicle fluid (SVF) in Corydoras remain unidentified. This study aimed to identify the SVF proteins in Corydoras aeneus and clarify the functions of the identified major SVF proteins. The SVF of this species was found to be highly viscous with a high protein concentration. Sperm motility was strongly suppressed in the presence of the SVF. We identified three SVF proteins - alpha-2-macroglobulin (A2M), carbonic anhydrase 12 (CA12) and lymphocyte antigen 6 (Ly6) - through RNA sequencing (RNA-Seq), LC-MS/MS and amino acid sequencing. Additionally, we found that the identified CA12, termed 'caCA12,' was degraded into about 10 kDa and 33 kDa polypeptides containing the CA domain. The 33 kDa polypeptide with the CA domain was found to inhibit sperm motility. The identified SVF proteins, including caCA12, may play a role in keeping sperm in an immotile state until they are close to the female ova, facilitating the remarkable sperm drinking reproductive process observed in C. aeneus.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reproductive steroids as potential mediators of parental-reproductive trade-offs in a brood parasitic species. 生殖类固醇作为一种寄生蜂亲代-生殖权衡的潜在媒介。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jeb.250044

Kathleen S Lynch, Elisha Henson

{"title":"Reproductive steroids as potential mediators of parental-reproductive trade-offs in a brood parasitic species.","authors":"Kathleen S Lynch, Elisha Henson","doi":"10.1242/jeb.250044","DOIUrl":"https://doi.org/10.1242/jeb.250044","url":null,"abstract":"Avian brood parasites display enhanced annual fecundity compared with other passerine birds. Female brood parasitic brown-headed cowbirds (Molothrus ater) lay a staggering estimated 40-50 eggs year-1. We examined how reproductive steroids mediate a possible trade-off between increased annual fecundity and parental care by comparing seasonal fluctuations in steroid profiles and follicular development in cowbirds and red-winged blackbirds (Agelaius phoeniceus), a related non-parasitic species. We also used gonadotropin-releasing hormone (GnRH) administration to determine whether species variation in GnRH responsivity reflects differences in behavioral phenotypes. These correlational and experimental studies are meant to test the hypothesis that reproductive steroid profiles have diverged in these two species, possibly in such a way that mediates a reproductive-parental trade-off in cowbirds. We identified several mechanisms that could enhance annual fecundity in cowbirds, and one mechanism that would do this at the cost of parental care: elevated testosterone. These results reveal that cowbirds exhibit earlier onset of breeding as measured by follicular size and estrogen concentration. Moreover, female cowbirds produce testosterone significantly quicker and more robustly in response to GnRH administration compared with red-winged blackbirds. Species divergence in seasonal steroid profiles and responsivity to GnRH, particularly with respect to testosterone, indicate the hypothalamic-pituitary-gonadal axis exhibits consequential modifications in cowbirds that may enhance reproductive output while also possibly simultaneously inhibiting caregiving behaviors.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus. 缺氧-复氧对大扇贝离体线粒体能量学和氧化应激的影响。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jeb.249870

Linda Lumor, Christian Bock, Felix Christopher Mark, Siriluck Ponsuksili, Inna Sokolova

{"title":"Effects of hypoxia-reoxygenation on the bioenergetics and oxidative stress in the isolated mitochondria of the king scallop, Pecten maximus.","authors":"Linda Lumor, Christian Bock, Felix Christopher Mark, Siriluck Ponsuksili, Inna Sokolova","doi":"10.1242/jeb.249870","DOIUrl":"https://doi.org/10.1242/jeb.249870","url":null,"abstract":"The king scallop (Pecten maximus) is a highly aerobic subtidal bivalve species vulnerable to fluctuations in oxygen availability. This study investigated the effects of short-term (15 min) and long-term (90 min) hypoxia-reoxygenation (H/R) stress on substrate-specific mitochondrial functions in the gill and digestive gland tissues of P. maximus, oxidizing substrates that engage mitochondrial Complex I (pyruvate, palmitate) and Complex II (succinate). Under normoxic conditions, scallop mitochondria preferentially oxidized pyruvate. H/R stress induced a significant decline in Complex I-driven ATP synthesis, increased proton leak and dysregulated fatty acid oxidation, indicating mitochondrial vulnerability to H/R stress. Following H/R, both tissues demonstrated a greater capacity for succinate oxidation than for Complex I substrates; however, long-term H/R exposure led to a reduction in respiratory coupling efficiency across all substrates. Notably, gill mitochondria exhibited more effective regulation of reactive oxygen species efflux and electron leak compared with digestive gland mitochondria under H/R stress. Despite these physiological changes, no evidence of oxidative damage was detected, suggesting the presence of a robust mitochondrial antioxidant defense. Collectively, these findings suggest that succinate oxidation plays an important role in stress recovery in P. maximus, providing insights into mitochondrial resilience and the management of oxidative stress during intermittent hypoxia.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0