Journal of Experimental Biology最新文献_第3页

A multi-scaling approach showing a transient metabolic mismatch in a freshwater fish (Zingel asper) during an acute heat stress. 一种多尺度方法显示了淡水鱼在急性热应激期间的短暂代谢不匹配。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 DOI: 10.1242/jeb.250202

Julia Watson, Chloé Souques, François-Xavier Dechaume-Moncharmont, Damien Roussel, Julie Leguyader, Rémy Lassus, Ludovic Guillard, Angeline Clair, Laëtitia Averty, Candice Bastianini, Lilian Redon, Anne Morales-Montaron, Yann Voituron, Martin Daufresne, Elisa Thoral, Loïc Teulier

{"title":"A multi-scaling approach showing a transient metabolic mismatch in a freshwater fish (Zingel asper) during an acute heat stress.","authors":"Julia Watson, Chloé Souques, François-Xavier Dechaume-Moncharmont, Damien Roussel, Julie Leguyader, Rémy Lassus, Ludovic Guillard, Angeline Clair, Laëtitia Averty, Candice Bastianini, Lilian Redon, Anne Morales-Montaron, Yann Voituron, Martin Daufresne, Elisa Thoral, Loïc Teulier","doi":"10.1242/jeb.250202","DOIUrl":"https://doi.org/10.1242/jeb.250202","url":null,"abstract":"Heat stress events will be more frequent and intense in the future. These events will challenge the capacity of organisms to exhibit sufficient metabolic flexibility to adapt to such variations. To better understand the acclimation processes implemented in response to acute warming, with an integrative approach we examined in vivo metabolic rate and cardiac mitochondrial respiration in the Rhône streber, during and after a heat stress on a precise timeline. The temperature was raised from 13°C to 18°C (+ 1°C per hour) and maintained at 18°C for 5 days, before returning to 13°C at the same rate. We repeatedly measured, during the heat stress and 5 days after the end of the event, in vivo metabolic rate in the same individuals and cardiac mitochondrial respiration from different individuals. At the organismal level, oxygen consumption increased in line with warming, and was subsequently followed by a return to pre-acclimated levels just after the end of the heat stress. At the opposite, cardiac mitochondrial respiration decreased during the heat stress, especially 24 hours in, and was recovered at the end of the event. Our results suggest that the heat stress was responsible for a metabolic mismatch in the strebers. Indeed, we observed (i) a strong thermodynamic effect without any acclimation process, suggesting that the range of temperatures chosen was not stressful for the fish, and (ii) the establishment of a transitory energy saving process. Our results underline the need for more integrative studies to understand how organisms will adapt to climate change.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143999648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The sticky truth: how spider predation success depends on their prey's body surface. 棘手的事实是：蜘蛛捕食成功与否取决于猎物的体表。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-08 DOI: 10.1242/jeb.249347

Lucas Baumgart, Sascha Schlüter, Marieke Moog, Annika Schönfeld, Adrian Heß, Florian Menzel, Anna-Christin Joel

{"title":"The sticky truth: how spider predation success depends on their prey's body surface.","authors":"Lucas Baumgart, Sascha Schlüter, Marieke Moog, Annika Schönfeld, Adrian Heß, Florian Menzel, Anna-Christin Joel","doi":"10.1242/jeb.249347","DOIUrl":"10.1242/jeb.249347","url":null,"abstract":"Spiders are prominent predators for insects, with which they have a close co-evolutionary history. Manifold capture techniques have evolved, with spider webs being one of most well-known traps in the world. Many webs include specialised threads, bearing either glue or cribellate nanofibres as adhesive to capture prey. Some webs, such as the sheet webs of Tarantulae, have no such intricate threads. The adhesion of gluey threads has been extensively studied already, but often on artificial surfaces. However, recent studies discovered that adhesion of cribellate nanofibres increases massively after contact with insect cuticular hydrocarbons (CHCs). This raises the question whether insect CHCs generally influence prey capture. We compared the adhesion of cribellate, ecribellate gluey and ecribellate non-specialised threads to either uncoated or CHC-coated foil, or native prey body surfaces. We found an influence of CHCs on all silken threads, but with different outcomes. CHC presence, its composition as well as the surface structure can impact the final adhesion force positively or negatively, depending on the thread type. In extreme cases, the adhesion was reduced to nearly zero (e.g. for gluey capture threads in contact with real prey). Thus, prey influence on adhesion is not limited to cribellate capture threads, but is a universal influence on adhesion of spider silken capture threads. Future studies should consider both insect surface chemistry and surface structure when assessing the effectiveness of capture thread types in an ecological and evolutionary context.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079663/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arduino allows development of a low-cost, high-resolution metabolic and behavioral phenotyping system for birds. Arduino允许开发低成本，高分辨率的鸟类代谢和行为表型系统。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-09 DOI: 10.1242/jeb.249921

Elizabeth J Rogers, Cory Elowe, Maria Stager, Alexander R Gerson

引用次数: 0

Encoding of antennal position and velocity by the Johnston's organ in hawkmoths. 天蛾触须位置和速度的约翰斯顿器官编码。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-02 DOI: 10.1242/jeb.249342

Chinmayee L Mukunda, Sanjay P Sane

{"title":"Encoding of antennal position and velocity by the Johnston's organ in hawkmoths.","authors":"Chinmayee L Mukunda, Sanjay P Sane","doi":"10.1242/jeb.249342","DOIUrl":"10.1242/jeb.249342","url":null,"abstract":"Insect antennae function as versatile, multimodal sensory probes in diverse behavioural contexts. In addition to their primary role as olfactory organs, they serve essential mechanosensory functions across insects, including auditory perception, vestibular feedback, airflow detection, gravity sensing and tactile sensation. These diverse functions are facilitated by the mechanosensory Johnston's organ (JO), located at the joint between the flagellum and the pedicel (second antennal segment). This joint lacks muscles, which means that JOs can perceive only passive deflections of the flagellum. Earlier work that characterized the sensitivity and short response time of the JO sensory units in hawkmoths showed that their sensitivity to a broad frequency range is range-fractionated. This vastly expands the functional repertoire of the JO. However, it is not clear what components of antennal kinematics are encoded by the JO. Here, we conducted experiments to test the hypothesis that JO neurons encode the position and velocity of angular movements of the flagellum. We recorded intracellularly from the axons of primary sensory neurons of the JO while stimulating it with ramp-and-hold stimuli in which either the antennal position or antennal angular velocity was maintained at various constant values. Our study shows that JO neurons encode angular velocity and position of the antenna in their response. We also characterized the neural adaptation of the responses to angular velocities and positions. The majority of neurons were sensitive to a movement in the ventrad direction, in the direction of gravity. The adaptation and directional response properties give rise to a nonlinear hysteresis-like response. Together, these findings highlight the neurophysiological basis underlying the functional versatility of the JO.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12079665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dive-by-dive variation in the diving respiratory air volume of southern elephant seals (Mirounga leonina). 南象海豹（miounga leonina）潜水呼吸气量随潜水的变化。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 DOI: 10.1242/jeb.249659

George Sato, Taiki Adachi, Christophe Guinet, Patrick Miller

{"title":"Dive-by-dive variation in the diving respiratory air volume of southern elephant seals (Mirounga leonina).","authors":"George Sato, Taiki Adachi, Christophe Guinet, Patrick Miller","doi":"10.1242/jeb.249659","DOIUrl":"https://doi.org/10.1242/jeb.249659","url":null,"abstract":"The role of diving respiratory air volume (DRAV) in deep-diving phocid seals remains poorly understood, largely due to the lack of methods for measuring DRAV in free-ranging divers that exhale before diving. We developed a method to estimate DRAV using a hydrodynamic glide model applied to descent glides recorded using multi-sensor data loggers. We estimated dive-by-dive DRAV for six negatively buoyant female southern elephant seals (Mirounga leonina). During shallow descent glides, rapid compression of DRAV influenced net buoyancy and gliding speed, making this phase suitable for estimating DRAV. Our results revealed dive-by-dive variation in DRAV, which was positively correlated with RMS sway acceleration (a proxy for per-stroke effort) and the depth at which gliding began during the initial descent. DRAV increased with both tissue density and maximum dive depth, suggesting that seals adjusted their DRAV to stay closer to neutral buoyancy through their dives. However, the observed level of adjustment did not result in neutral buoyancy at half of the maximum dive depth, as predicted to minimise round-trip locomotion costs. Instead, the seals typically adjusted DRAV to reach neutral buoyancy at ∼30 m depth,<10% of their mean maximum dive depth. This indicates that strong negative tissue density imposes transit costs that cannot be fully compensated by DRAV adjustment alone. Future work should explore whether other breath-hold divers show similar patterns of DRAV adjustment and quantify the associated physiological and ecological benefits.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transcriptomic responses to within-generation and intergenerational environmental warming in a cold-adapted salmonid. 冷适应鲑鱼对代际和代内环境变暖的转录组反应。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-02 DOI: 10.1242/jeb.249415

Chantelle M Penney, Gary Burness, Gerardo Zapata, François Lefebvre, Chris C Wilson

{"title":"Transcriptomic responses to within-generation and intergenerational environmental warming in a cold-adapted salmonid.","authors":"Chantelle M Penney, Gary Burness, Gerardo Zapata, François Lefebvre, Chris C Wilson","doi":"10.1242/jeb.249415","DOIUrl":"10.1242/jeb.249415","url":null,"abstract":"Cold-adapted species are particularly threatened by climate change as rates of environmental warming outpace the ability of many populations to adapt. Recent evidence suggests that intergenerational thermal plasticity may play a role in the response of cold-adapted organisms to long-term changes in temperature. Using RNA sequencing, we explored differential gene expression of lake trout (Salvelinus namaycush), a cold-adapted species, to examine the molecular processes that respond to elevated temperature under conditions of within-generation (offspring) and intergenerational (parental) warm acclimation. We hypothesized that genes associated with metabolism, growth and thermal stress/tolerance would be differentially expressed in juvenile lake trout offspring depending on their own acclimation temperature and that of their parents. While parental warm acclimation did have an intergenerational effect on gene expression in their offspring, within-generation (offspring) warm acclimation had a greater effect on the number of differentially expressed genes. Differentially expressed genes enriched pathways for thermal stress, signalling processes, immune function and transcription regulation and depended on the acclimation temperature of the offspring in isolation or in combination with parental warm acclimation. Despite evidence of intergenerational effects on gene expression in lake trout in response to elevated temperatures, the effect is unlikely to significantly increase populations' ability to cope with increasing environmental temperatures associated with climate change.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The missing effect of temperature on branchial O2 loss in an air-breathing catfish. 温度对呼吸鲶鱼鳃部氧损失的缺失效应。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jeb.250295

Sara L Jensen, Magnus L Aaskov, Hans Malte, Mark Bayley

引用次数: 0

Sublethal glyphosate exposure reduces honey bee foraging and alters the balance of biogenic amines in the brain. 接触亚致死的草甘膦会减少蜜蜂的觅食，并改变大脑中生物胺的平衡。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-06 DOI: 10.1242/jeb.250124

Laura C McHenry, Roger Schürch, McAlister Council-Troche, Aaron D Gross, Lindsay E Johnson, Bradley D Ohlinger, Margaret J Couvillon

{"title":"Sublethal glyphosate exposure reduces honey bee foraging and alters the balance of biogenic amines in the brain.","authors":"Laura C McHenry, Roger Schürch, McAlister Council-Troche, Aaron D Gross, Lindsay E Johnson, Bradley D Ohlinger, Margaret J Couvillon","doi":"10.1242/jeb.250124","DOIUrl":"https://doi.org/10.1242/jeb.250124","url":null,"abstract":"Glyphosate is a broad-spectrum herbicide that inhibits the shikimate pathway, which honey bees (Apis mellifera), a non-target beneficial pollinator, do not endogenously express. Nonetheless, sublethal glyphosate exposure in honey bees has been correlated to impairments in gustation, learning, memory and navigation. While these impacted physiologies underpin honey bee foraging and recruitment, the effects of sublethal glyphosate exposure on these important behaviors remain unclear, and any proximate mechanism of action in the honey bee is poorly understood. We trained cohorts of honey bees from the same hives to forage at one of two artificial feeders offering 1 mol l-1 sucrose solution, either unaltered (N=40) or containing glyphosate at 5 mg acid equivalent (a.e.) l-1 (N=46). We then compared key foraging behaviors and, on a smaller subset of bees, recruitment behaviors. Next, we quantified protein levels of octopamine, tyramine and dopamine, and levels of the amino acid precursor tyrosine in the brains of experimental bees collected 3 days after the exposure. We found that glyphosate treatment bees reduced their foraging by 13.4% (P=0.022), and the brain content of tyramine was modulated by a crossover interaction between glyphosate treatment and the number of feeder visits (P=0.004). Levels of octopamine were significantly correlated with its precursors tyramine (P=0.011) and tyrosine (P=0.018) in glyphosate treatment bees, but not in control bees. Our findings emphasize the critical need to investigate impacts of the world's most-applied herbicide and to elucidate its non-target mechanism of action in insects to create better-informed pollinator protection strategies.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of back muscle fatigue and modified trunk inertia on lumbar kinematics, kinetics and muscle activity during walking. 背部肌肉疲劳和躯干惯性改变对行走时腰椎运动学、动力学和肌肉活动的影响。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-09 DOI: 10.1242/jeb.250479

Benjamin E Sibson, Andrew K Yegian, Ali Yawar, Daniel E Lieberman

{"title":"Effects of back muscle fatigue and modified trunk inertia on lumbar kinematics, kinetics and muscle activity during walking.","authors":"Benjamin E Sibson, Andrew K Yegian, Ali Yawar, Daniel E Lieberman","doi":"10.1242/jeb.250479","DOIUrl":"https://doi.org/10.1242/jeb.250479","url":null,"abstract":"Back endurance is a strong predictor of back pain, but the mechanisms underlying this relationship are not clear. Fatigue reduces muscles' force-generating capacity, so greater fatigability may increase lumbar motion and loading and trunk muscle co-contraction. Using a novel pack to modify inertia, we tested the effect of back fatigue and increased trunk inertia on lumbar kinematics, kinetics and muscle activity during walking. Lumbar kinematic and kinetic amplitudes and maximum muscle activity were measured per stride across four conditions: pre- and post-fatigue, with and without increased trunk inertia. The pack caused increases in maximum lumbar erector spinae (ES) activity by 3.19 times the average value calculated during the pre-fatigue no-pack trial (P<0.001), amplitude of lumbar flexion-extension moment by 0.0189 N m (kg g m)-1 (P<0.001), lumbar lateral bending moment by 0.0028 N m (kg g m)-1 (P=0.019) and lumbar axial rotation moment by 0.0203 N m (kg g m)-1 (P<0.001), and decreases in the amplitude of roll angle by 1.31 deg and yaw angle by 6.65 deg (both P<0.001). Back endurance is positively associated with change in maximum lumbar ES activity (r=0.69, P=0.013) and negatively associated with change in maximum rectus abdominus (RA) activity (r=-0.72, P=0.008) and lumbar flexion-extension moment amplitude (r=-0.62, P=0.031). Overall, individuals with less back endurance had increased maximum RA activity and sagittal kinetics post-fatigue whereas individuals with higher back endurance showed the opposite response. Increased RA activity with less back endurance may be a protective mechanism for stabilizing the trunk in response to increased sagittal lumbar loading due to fatigue.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143995887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bumblebees increase their learning flight altitude in dense environments. 大黄蜂在密集环境中提高学习飞行高度。

IF 2.8 2区生物学

Journal of Experimental Biology Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jeb.249714

Annkathrin Sonntag, Mathieu Lihoreau, Olivier J N Bertrand, Martin Egelhaaf

{"title":"Bumblebees increase their learning flight altitude in dense environments.","authors":"Annkathrin Sonntag, Mathieu Lihoreau, Olivier J N Bertrand, Martin Egelhaaf","doi":"10.1242/jeb.249714","DOIUrl":"https://doi.org/10.1242/jeb.249714","url":null,"abstract":"Bumblebees rely on visual memories acquired during the first outbound flights to relocate their nest. While these learning flights have been extensively studied in sparse environments with few objects, little is known about how bees adapt their flight in more dense, cluttered, settings that better mimic their natural habitats. Here, we investigated how environmental complexity influences the first outbound flights of bumblebees. In a large arena, we tracked the bees' 3D positions to examine the flight patterns, body orientations and nest fixations across environmental conditions characterised by different object constellations around the nest entrance. In cluttered environments, bees prioritised altitude gain over horizontal distance, suggesting a strategy to overcome obstacles and visual clutter. Body orientation patterns became more diverse in dense environments, indicating a balance between nest-oriented learning and obstacle avoidance. Notably, bees consistently preferred to fixate the location of the nest entrance from elevated positions above the dense environment across all conditions. Our results reveal significant changes in 3D flight structure, body orientation and nest fixation behaviours as object density increases. This highlights the importance of considering 3D space and environmental complexity in understanding insect navigation.","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":"228 9","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0