{"title":"Arousal from hibernation increases blood oxygen saturation in 13-lined ground squirrels.","authors":"Brynne M Duffy, Catherine M Ivy, James F Staples","doi":"10.1242/jeb.249830","DOIUrl":"https://doi.org/10.1242/jeb.249830","url":null,"abstract":"<p><p>Hibernating Ictidomys tridecemlineatus, 13-lined ground squirrels (TLGS), are considered models of ischemia-reperfusion tolerance, as both tissues and isolated mitochondria withstand anoxia followed by rapid re-oxygenation in vitro. This tolerance is likely adaptive, protecting against damage during the numerous arousals from torpor throughout the hibernation season. O2 availability is likely low during torpor, but suppressed metabolism lowers O2 demand, potentially mitigating hypoxic stress. During arousal to interbout euthermia (IBE), heart rate, blood pressure, and ventilation increase rapidly, suggesting increased O2 availability, but tissue oxygenation has not been measured during arousal or IBE in TLGS. Using pulse-oximetry collars, we characterized dramatic increases in O2 availability during arousal; carotid artery O2 saturation rose from as low as 35% early in arousal to 87% during IBE. These changes closely followed rising heart rate. Our results demonstrate that hibernating TLGS survive profound O2 deprivation early in arousal and rapid O2 influx as arousal progresses.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143719740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Adam B Grimmitt, Maeve E Whelan, Douglas N Martini, Wouter Hoogkamer
{"title":"Walking with increased step length variability increases the metabolic cost of walking in young adults.","authors":"Adam B Grimmitt, Maeve E Whelan, Douglas N Martini, Wouter Hoogkamer","doi":"10.1242/jeb.250126","DOIUrl":"10.1242/jeb.250126","url":null,"abstract":"<p><p>Several studies have observed a relationship between step length variability and the metabolic cost of walking. In these studies, changes in step length variability were secondary to changes in walking speed or step width variability. The purpose of this study was to determine how directly increasing step length variability affects the metabolic cost of walking. Eighteen healthy young adults completed 5-minute trials of treadmill walking at 1.20 m/s while we manipulated their step length variability. Illuminated rectangles were projected onto the surface of a treadmill to cue step length variabilities of 0, 5 and 10% coefficient of variation. Step length and its variability were tracked with reflective markers on the feet. Metabolic power across habitual (no projections) and the three variability conditions was measured using indirect calorimetry and analyzed using linear mixed effects modeling. Metabolic power was largest in the 10% condition (4.30±0.23 W/kg) compared to 0% (4.16±0.18 W/kg) and habitual (3.98±0.25 W/kg). Actual step length variability was significantly different from prescribed: 0% (3.17±0.64%), 5% (4.38±0.98%) and 10% (6.94±1.07%) conditions. For every 1% increase in step length variability, there was a 1.1% (0.05 W/kg; p<0.001) increase in metabolic power. Our results demonstrate an association between the metabolic cost of walking and step length variability. This suggests that increased gait variability contributes to a small portion of the increased cost of walking seen in older adults and neurological populations.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143700564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Brett M Culbert, Stephen D McCormick, Nicholas J Bernier
{"title":"Osmoregulatory contributions of the corticotropin-releasing factor system in the intestine of Atlantic salmon.","authors":"Brett M Culbert, Stephen D McCormick, Nicholas J Bernier","doi":"10.1242/jeb.250052","DOIUrl":"https://doi.org/10.1242/jeb.250052","url":null,"abstract":"<p><p>The ability of euryhaline fishes to tolerate different environmental salinities depends upon the flexibility of their osmoregulatory organs, including the intestine. Several endocrine pathways contribute to the coordination of osmoregulatory processes in the teleost intestine; however, while the corticotropin-releasing factor (CRF) system has established osmoregulatory actions in the mammalian intestine, it is unclear whether the intestinal CRF system serves similar functions in teleosts. Therefore, we sought to determine whether the CRF system contributes to osmoregulatory processes in the intestine of Atlantic salmon (Salmo salar). We first showed using in vitro sac preparations that activation of CRF receptor type 2 (CRFR2) in the middle and posterior regions of the intestine reduces water, Na+, and Cl- absorption. However, co-activation of CRFR1 and CRFR2 inhibited water and Na+ absorption without affecting net Cl- absorption. We then assessed how the CRF system in the middle and posterior regions of the intestine was transcriptionally regulated during the seasonal acquisition of seawater tolerance (i.e., smoltification) and following changes in environmental salinity. Smolts had higher transcript levels of CRF ligands than parr and this difference persisted following seawater transfer. Additionally, seawater transfer caused transient increases in transcription of urocortin 2 (UCN2) and CRFR2 (posterior intestine only). Similar increases in UCN2 and CRFR2 were observed following seawater to freshwater transfer of post-smolts. Our results indicate that the intestinal CRF system of Atlantic salmon contributes to osmoregulation during the initial days following changes in environmental salinity and that osmoregulatory actions of the intestinal CRF system are conserved across vertebrates.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Multigenerational exposure to glyphosate has only modest effects on life history traits, stress tolerance, and microbiome in a field cricket.","authors":"J W Whitlock, P M Orwin, Z R Stahlschmidt","doi":"10.1242/jeb.250210","DOIUrl":"https://doi.org/10.1242/jeb.250210","url":null,"abstract":"<p><p>Glyphosate (GLY) is the most used herbicide worldwide, and it can be toxic to off-target species, such as insects. While GLY-based herbicides (GBHs) can influence insect microbiomes, little is known about its cascading effects on fitness-related traits, such as life history or stress tolerance, especially in the context of long-term, multigenerational exposure. Thus, we exposed the variable field cricket, Gryllus lineaticeps, to GBH within- and across-generations to examine the potential role of GBH in developmental plasticity and evolution. Specifically, we measured its effects on life-history traits (e.g., developmental duration, adult body size and mass, and a life-history tradeoff between investment into reproduction and flight), stress (heat and desiccation) tolerance, and the gut microbiome. One generation of exposure to GBH reduced desiccation tolerance, which was also lower in flight-capable individuals. However, after 11 generations of exposure to GBH, this cost of GBH disappeared, and GBH exposure instead increased adult body size and mass in flight-incapable individuals. Flight capacity had a stronger effect on the gut bacterial community than GBH exposure where flight-capable individuals contained more than twice as many Family Oscillospiraceae and fewer than half as many Family Erysipelotrichaceae. The effects of both flight capacity and GBH on the microbiome were only evident in Generation 1. Together, our results indicate that GBH exposure may have quite modest long-term effects on stress tolerance and the gut microbiome. However, GBH may facilitate the evolution of flightlessness given its potential benefits to flight-incapable individuals, which exhibit greater reproductive potential and tolerance to climate stressors compared to flight-capable individuals.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Gene expression and enzyme activity analysis of carbohydrate digestion in Strongylocentrotus purpuratus larvae.","authors":"Jasper Hildebrand, Meike Stumpp","doi":"10.1242/jeb.250125","DOIUrl":"https://doi.org/10.1242/jeb.250125","url":null,"abstract":"<p><p>Carbohydrates play multifaceted roles in marine ecosystems, serving as structural components in algae, energy storage molecules, and vital nutrients for marine organisms. The purple sea urchin Strongylocentrotus purpuratus undergoes metamorphosis during ontogeny, transitioning its feeding strategy from microalgae to macroalgae as the primary food source. However, the digestive mechanisms underlying carbohydrate digestion in sea urchin larvae remain poorly understood. We investigated the carbohydrate digestion capabilities of S. purpuratus larvae, using expression level analysis of candidate genes putatively involved in carbohydrate digestion, quantification of enzyme activity and characterization (pH and temperature optima) for the digestion of starch, laminarin, cellulose, xylan, and trehalose. Transcriptomic analyses revealed the expression of genes encoding putative carbohydrate-degrading enzymes during early larval development. RT-qPCR demonstrated age- and/or feeding-dependent expression patterns of glycosidase candidate genes β-1,3-glucanase (laminarinase), α-amylase, endo-ß-1,4-glucanase D-like (cellulase), xylanase/β-glucanase-like and trehalase. Furthermore, enzymatic assays elucidated differential temporal patterns, and thermal and pH optima of associated carbohydrate-degrading enzymes. A comparison of the enzymatic degradation of five substrates demonstrated that laminarinase activity is five times higher than the enzyme activities involved in digesting starch, cellulose, xylan, and trehalose, opening up a hypothesis regarding the importance of laminarin for larval growth.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143674227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Dan J Hayman, Lola M Morrin, Sudipta Halder, Eleanor J Phillips, Mirre J P Simons, Iwan R Evans
{"title":"Expansion of Drosophila haemocytes using a conditional GeneSwitch driver affects larval haemocyte function, but does not modulate adult lifespan or survival after severe infection.","authors":"Dan J Hayman, Lola M Morrin, Sudipta Halder, Eleanor J Phillips, Mirre J P Simons, Iwan R Evans","doi":"10.1242/jeb.249649","DOIUrl":"https://doi.org/10.1242/jeb.249649","url":null,"abstract":"<p><p>Macrophages are responsible for diverse and fundamental functions in vertebrates. Drosophila blood cells (haemocytes) are dominated by cells bearing a striking homology to vertebrate macrophages (plasmatocytes). The importance of haemocytes has been demonstrated previously, with immune and developmental phenotypes observed upon haemocyte ablation. Here we show that we can increase Hml-positive cell numbers using a constitutively-active form of ras and ablate Hml-positive cell numbers using the pro-apoptotic transgene bax. However, in adults, compared to larvae, total blood cell numbers were not significantly affected by experimental expansion or ablation, implying the existence of feedback mechanisms regulating haemocyte numbers. No effect on lifespan was observed from driving ras and bax in Hml-positive cells via a conditional approach (Hml-GeneSwitch). Using constitutive expression, we observed differences in lifespan, however we attribute this to differences in genetic background. Additionally, no effect of either transgene was observed upon infection with a high dose of two different bacterial species, although pupal lethality was observed upon expansion of Hml-positive cells in a self-encapsulation mutant genetic background. The latter confirms that changes in Hml-positive cell numbers can result in phenotypes. The lack of adult phenotypes could be due to the strength of experimental manipulations or compensation via feedback mechanisms operating to regulate total blood cell numbers. Our study demonstrates the importance of conditional approaches to modulate haemocyte cell numbers, allowing for more precise study of innate immune function. This strategy could be especially fruitful to uncover mechanisms regulating total blood cell numbers across development and ageing.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143670041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alexa R Cesari, Jesse K Placone, Nicole L Ramo, Michael V Rosario, Matthew Morris, Danielle S Adams, Frank E Fish
{"title":"Comparative mechanical and elastic properties of the doral and ventral tendons in the peduncle of harbor porpoise (Phocoena phocoena).","authors":"Alexa R Cesari, Jesse K Placone, Nicole L Ramo, Michael V Rosario, Matthew Morris, Danielle S Adams, Frank E Fish","doi":"10.1242/jeb.249621","DOIUrl":"https://doi.org/10.1242/jeb.249621","url":null,"abstract":"<p><p>Cetaceans swim via vertical movements of the tail. The tendons located in the caudal peduncle are attached to the caudal vertebrae to generate propulsive oscillations. Arguments on whether the upstrokes and downstrokes of the tail and propulsive flukes are symmetrical or asymmetrical in time. Previous research from kinematics of swimming animals, muscle architecture, and histology has supported both conditions. The composition and structure of the tendons, however, suggest a potential mechanism to evaluate this disparity. In this study, the tendons of the caudal peduncle of the harbor porpoise (Phocoena phocoena), specifically the extensor caudae medialis (ECM) and the extensor caudae lateralis (ECL) from the epaxial muscle, and the medial hypaxialis lumborum (MHL) from the hypaxial muscle were mechanically tested. Ramp to failure was performed on isolated tendon fascicles. Stress relaxation tests to 3% strain were also performed on fascicles. Polarized light microscopy was used to visualize the fibril crimp as tensile forces were applied to fascicles. Uncrimping of isolated fascicles was visualized at mean strain values between 0.031% and 0.048%. The maximum elastic moduli of fascicles taken to failure were between 1039.5 and1185.8 MPa. No differences were found in the mechanical performance of the fascicles of the epaxial and hypaxial tendons. The mechanical properties of peduncle fascicles suggest a symmetrical stroke cycle for swimming by the porpoise.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Angus B Thies, Maitri R Paul, Daniel Wangpraseurt, Martin Tresguerres
{"title":"Co-option of immune and digestive cellular machinery to support photosymbiosis in amoebocytes of the upside-down jellyfish Cassiopea xamachana.","authors":"Angus B Thies, Maitri R Paul, Daniel Wangpraseurt, Martin Tresguerres","doi":"10.1242/jeb.249849","DOIUrl":"https://doi.org/10.1242/jeb.249849","url":null,"abstract":"<p><p>The upside-down jellyfish, Cassiopea spp., host their algal symbionts inside a subset of amoebocytes, phagocytic cells that also play innate immune functions akin to macrophages from vertebrate animals. Amoebocyte precursors phagocytose algae from the jellyfish gut and store them inside intracellular compartments called symbiosomes. Subsequently, the precursors migrate to the mesoglea, differentiate into symbiotic amoebocytes, and roam throughout the jellyfish body where the algae remain photosynthetically active and supply the jellyfish host with a significant portion of their organic carbon needs. Here, we show that the amoebocyte symbiosome membrane contains V-H+-ATPase (VHA), the proton pump that acidifies phagosomes and lysosomes in all eukaryotes. Many symbiotic amoebocytes also abundantly express a carbonic anhydrase (CA), an enzyme that reversibly hydrates CO2 into H+ and HCO3-. Moreover, we found that the symbiosome lumen is pronouncedly acidic and that pharmacological inhibition of VHA or CA activities significantly decreases photosynthetic oxygen production in live jellyfish. These results point to a carbon concentrating mechanism (CCM) that co-opts VHA and CA from the phago-lysosomal machinery that ubiquitously mediates food digestion and innate immune responses. Analogous VHA-dependent CCMs have been previously described in reef-building corals, anemones, and giant clams; however, these other two cnidarians host their dinoflagellate algae inside gastrodermal cells -not in amoebocytes- and the clam hosts theirs within the gut lumen. Thus, our study identifies an example of convergent evolution at the cellular level that might broadly apply to invertebrate-microbe photosymbioses while also providing evolutionary links with intra- and extracellular food digestion and the immune system.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143662531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Encoding of antennal position and velocity by the Johnston's organ in hawkmoths.","authors":"Chinmayee L Mukunda, Sanjay P Sane","doi":"10.1242/jeb.249342","DOIUrl":"https://doi.org/10.1242/jeb.249342","url":null,"abstract":"<p><p>Insect antennae function as versatile, multimodal sensory probes in diverse behavioural contexts. In addition to their primary role as olfactory organs, they serve essential mechanosensory functions across insects, including auditory perception, vestibular feedback, airflow detection, gravity sensing, and tactile sensation. These diverse functions are facilitated by the mechanosensory Johnston's organ (JO), located at the joint between the flagellum and the second antennal segment i.e. the pedicel. The pedicel-flagellum joint lacks muscles which means that the Johnston's organs can perceive only passive deflections of the flagellum. Earlier work which characterized the sensitivity and short response time of the sensory units of JO in hawkmoths, showed that their sensitivity to a broad frequency range is range-fractionated. This vastly expands the functional repertoire of the JO. However, it is not clear what components of antennal kinematics are encoded by the JO. Here, we conducted experiments to test the hypothesis that JO neurons encode the position and velocity of angular movements of the flagellum. We recorded intracellularly from the axons of primary sensory neurons of JO while stimulating it with ramp-and-hold stimuli in which either the antennal position or the antennal angular velocity was maintained at various constant values. Our study shows that JO neurons encode angular velocity and position of the antenna in their response. We also characterized the neural adaptation of the responses to angular velocities and positions. A majority of neurons were sensitive to a movement in the ventrad direction, in the direction of gravity. The adaptation and the directional response properties give rise to a nonlinear hysteresis-like response. Together, these findings highlight the neurophysiological basis underlying the functional versatility of the JO.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143649046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Patrick M Mineo, Cameron J World, T A Morris, Nancy J Berner
{"title":"Dietary modification of membrane composition mimics characteristics of thermal acclimation in the Eastern newt (Notophthalmus viridescens).","authors":"Patrick M Mineo, Cameron J World, T A Morris, Nancy J Berner","doi":"10.1242/jeb.249613","DOIUrl":"https://doi.org/10.1242/jeb.249613","url":null,"abstract":"<p><p>Acclimation in response to seasonal temperature fluctuations is well described across animal taxa. Our previous studies on adult Eastern red spotted newts have demonstrated that winter or cold-acclimated newts prefer lower cloacal temperatures, have higher standard metabolic rates (SMR), exhibit higher skeletal muscle cytochrome C oxidase (CCO) and citrate synthase (CS) activity, and possess membranes composed of elevated polyunsaturated fatty acid (PUFA) content compared to skeletal muscle of summer or warm-acclimated newts. Acclimation to cold also results in partial compensation of locomotor performance. Additionally, northern populations have higher CS and CCO activity and a higher degree of membrane unsaturation compared to southern populations regardless of acclimation conditions. In the present study we tested the hypothesis that modification of membrane FA composition mimics characteristics of thermal acclimation. We modified membrane composition in newts independent of environmental temperature by feeding them diets differing in FA composition (saturated, monounsaturated, and n3 or n6 PUFA diets) and measured CCO and CS activity, SMR, preferred cloacal temperature, locomotor performance and thermal tolerance. Here we present data suggesting that a diet-mediate elevation of PUFA in tissue membranes results in lower proffered body temperature, increased metabolic rate, increased burst speed at low temperature, and decreased burst speed at high temperature. This introduces an ectothermic vertebrate model system that acclimates characteristics across levels of biological organization in which we can effectively uncouple membrane composition from environmental temperature or light cycle, and further suggests that diet may be an important component of thermal acclimation in nature.</p>","PeriodicalId":15786,"journal":{"name":"Journal of Experimental Biology","volume":" ","pages":""},"PeriodicalIF":2.8,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143657479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}