Journal of Functional Biomaterials最新文献

{"title":"Innovative Strategies and Methodologies in Antimicrobial Peptide Design.","authors":"Devesh Pratap Verma, Amit Kumar Tripathi, Ashwani Kumar Thakur","doi":"10.3390/jfb15110320","DOIUrl":"10.3390/jfb15110320","url":null,"abstract":"<p><p>Multiple lines of research have led to the hypothesis that antimicrobial peptides (AMPs) are an important component of the innate immune response, playing a vital role in the defense against a wide range of infectious diseases. In this review, we explore the occurrence and availability of antimicrobial proteins and peptides across various species, highlighting their natural abundance and evolutionary significance. The design of AMPs has been driven by the identification of key structural and functional features, which are essential for optimizing their antimicrobial activity and reducing toxicity to host cells. We discuss various approaches, including rational design, high-throughput screening, and computational modeling, that have been employed to develop novel AMPs with enhanced efficacy. A particular focus is given to the identification and characterization of peptide fragments derived from naturally occurring host defense proteins, which offer a promising avenue for the discovery of new AMPs. The incorporation of artificial intelligence (AI) and machine learning (ML) tools into AMP research has further accelerated the identification, optimization, and application of these peptides. This review also discusses the current status and therapeutic potential of AMPs, emphasizing their role in addressing the growing issue of antibiotic resistance. The conclusion highlights the importance of continued research and innovation in AMP development to fully harness their potential as next-generation antimicrobial agents.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595219/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing Performance of Silicone Hydrogel Contact Lenses with Hydrophilic Polyphenolic Coatings.","authors":"Paul Demian, Daichi Nagaya, Roeya Refaei, Kaoru Iwai, Daiki Hasegawa, Masaki Baba, Phillip B Messersmith, Mouad Lamrani","doi":"10.3390/jfb15110321","DOIUrl":"10.3390/jfb15110321","url":null,"abstract":"<p><p>This study explores the application of a dopamine-assisted co-deposition strategy to modify the surface of daily disposable silicone hydrogel contact lenses. Aiming to enhance the hydrophilicity of these typically hydrophobic lenses, we developed an industry-friendly process using simple dip coating in aqueous solutions. By co-depositing tannic acid, dopamine and chitosan derivative and employing periodate oxidation, we achieved a rapid and efficient coating process. High-molecular-weight branched polyethylene imine was introduced to promote surface reactions. The resulting lenses exhibited extreme hydrophilicity and lipid repellency without compromising their intrinsic properties or causing cytotoxicity. While the coating demonstrated partial antimicrobial activity against Gram-positive Staphylococcus aureus, it offers a foundation for the further development of broad-spectrum antimicrobial coatings. This versatile and efficient process, capable of transforming hydrophobic contact lenses into hydrophilic ones in just 15 min, shows significant potential for improving comfort and performance in daily disposable contact lenses.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595945/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sericin Protein: Structure, Properties, and Applications.","authors":"Rony Aad, Ivana Dragojlov, Simone Vesentini","doi":"10.3390/jfb15110322","DOIUrl":"10.3390/jfb15110322","url":null,"abstract":"<p><p>Silk sericin, the glue protein binding fibroin fibers together, is present in the <i>Bombyx mori</i> silkworms' cocoons. In recent years, sericin has gained attention for its wide range of properties and possible opportunities for various applications, as evidenced by the meta-analysis conducted in this review. Sericin extraction methods have evolved over the years to become more efficient and environmentally friendly, preserving its structure. Due to its biocompatibility, biodegradability, anti-inflammatory, antibacterial, antioxidant, UV-protective, anti-tyrosinase, anti-aging, and anti-cancer properties, sericin is increasingly used in biomedical fields like drug delivery, tissue engineering, and serum-free cell culture media. Beyond healthcare, sericin shows promise in industries such as textiles, cosmetics, and food packaging. This review aims to highlight recent advancements in sericin extraction, research, and applications, while also summarizing key findings from earlier studies.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595228/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penetration of Non-Adhesive Gel-like Embolic Materials During Dural Vessels Embolization According to Characteristics of Tantalum Powder.","authors":"Andrey Petrov, Arkady Ivanov, Sergei Ermakov, Egor Kolomin, Anna Petrova, Oleg Belokon, Konstantin Samochernykh, Larisa Rozhchenko","doi":"10.3390/jfb15110319","DOIUrl":"10.3390/jfb15110319","url":null,"abstract":"<p><p>Tantalum powder is included in the composition of Non-Adhesive Gel-like Embolic Materials (NAGLEMs) for X-ray opacity. The duration of X-ray opacity during embolization is primarily associated with the particle size, which differs in the most used NAGLEMs-ONYX (Medtronic) and SQUID (Balt). NAGLEMs are widely used for the embolization of branches of the middle meningeal artery (MMA) in patients with chronic subdural hematomas (CSDHs). Considering the size (5-15 microns) of the target dural vessels, we assumed that not only the viscosity of NAGLEMs, but also the size and shape of tantalum granules may be important for the penetration of these gel-like embolic agents and determine their behavior. A notable discrepancy in size was observed. The medium-sized granules in the SQUID 18 sample (0.443 ± 0.086 microns, M ± SD) were found to be approximately ten times smaller than the tantalum granules in the ONYX 18 sample (5.2 ± 0.33 microns, M ± SD).Tantalum granules in SQUID 18 have a regular spherical shape; in ONYX 18 they have an irregular angular shape. When comparing the behavior of gel-like embolic agents of the same viscosity during MMA embolization in patients with CSDHs (an average age of 62.2 ± 14.3 years) in the group where SQUID 18 (<i>n</i> = 8) was used, the gel-like embolic agent in dural vessels demonstrated significantly greater penetration ability compared with the group where ONYX 18 (<i>n</i> = 8) was used. Accordingly, not only the viscosity of NAGLEMs, but also the size and shape of tantalum granules can have a significant effect on the penetration ability of gel compositions.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advances in the Preparation, Antibacterial Mechanisms, and Applications of Chitosan.","authors":"Kunjian Wu, Ziyuan Yan, Ziyang Wu, Jiaye Li, Wendi Zhong, Linyu Ding, Tian Zhong, Tao Jiang","doi":"10.3390/jfb15110318","DOIUrl":"10.3390/jfb15110318","url":null,"abstract":"<p><p>Chitosan, a cationic polysaccharide derived from the deacetylation of chitin, is widely distributed in nature. Its antibacterial activity, biocompatibility, biodegradability, and non-toxicity have given it extensive uses in medicine, food, and cosmetics. However, the significant impact of variations in the physicochemical properties of chitosan extracted from different sources on its application efficacy, as well as the considerable differences in its antimicrobial mechanisms under varying conditions, limit the full realization of its biological functions. Therefore, this paper provides a comprehensive review of the structural characteristics of chitosan, its preparation methods from different sources, its antimicrobial mechanisms, and the factors influencing its antimicrobial efficacy. Furthermore, we highlight the latest applications of chitosan and its derivatives across various fields. We found that the use of microbial extraction shows promise as a new method for producing high-quality chitosan. By analyzing the different physicochemical properties of chitosan from various sources and the application of chitosan-based materials (such as nanoparticles, films, sponges, and hydrogels) prepared using different methods in biomedicine, food, agriculture, and cosmetics, we expect these findings to provide theoretical support for the broader utilization of chitosan.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595984/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-Scale Characterization of Conventional and Immediate Dental Implant Systems.","authors":"Seeun Mok, Mori E Naftulin, Luiz Meirelles, Minji Kim, Jie Liu, Christine H Lee, Hany A Emam, Courtney A Jatana, Hua-Hong Chien, Ching-Chang Ko, Do-Gyoon Kim","doi":"10.3390/jfb15110317","DOIUrl":"10.3390/jfb15110317","url":null,"abstract":"<p><p>We hypothesized that the different post-implantation healing stages between the conventional and immediate implantations produce different amounts and tissue composition of the peri-implant bone. Thus, the objective of the current study was to examine whether the stability of dental implant systems is associated with characteristics of the interfacial bone area at different post-implanation healing periods. Mandibular molars were extracted from each beagle dog. After 10 weeks post-extraction, a screw-type titanium dental implant was placed in the molar location following a conventional dental implant (<i>Con</i>) procedure. Simultaneously, mandibular premolars were extracted and the same type of dental implant was placed in the distal site of the extracted premolar root following an immediate dental implant (<i>Imm</i>) procedure. The implant stability quotient (ISQ) values were not significantly different between <i>Con</i> and <i>Imm</i> groups at 0-, 3-, and 6-weeks post-implantation. However, 3D micro-computed tomography and 2D histological images confirmed that the <i>Imm</i> system had more gaps between the bone and implant than the <i>Con</i> system. On the other hand, the nanoindentation modulus value at the bone-implant interface was significantly higher for the <i>Imm</i> group than the <i>Con</i> group at both 3 weeks and 6 weeks post-implantation. The current results from multi-scale characterization suggest that the higher interfacial bone quality of the <i>Imm</i> system, despite its earlier post-implantation stage, plays a crucial role in maintaining stability comparable to that of the <i>Con</i> system.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium Alginate Microneedles Loaded with Vancomycin for Skin Infections.","authors":"Juhaina M Abu Ershaid, Han Zhang, May Tayyem, Akmal H Sabri, Ryan F Donnelly, Lalitkumar K Vora","doi":"10.3390/jfb15110316","DOIUrl":"10.3390/jfb15110316","url":null,"abstract":"<p><strong>Background: </strong>Skin and soft tissue infections (SSTIs) present significant treatment challenges. These infections often require systemic antibiotics such as vancomycin, which poses a risk for increased bacterial resistance. Topical treatments are hindered by the barrier function of the skin, and microneedles (MNs) offer a promising solution, increasing patient compliance and negating the need for traditional needles.</p><p><strong>Methods: </strong>This study focused on the use of sodium alginate MNs for vancomycin delivery directly to the site of infection via a cost-effective micromolding technique. Dissolving polymeric MNs made of sodium alginate and loaded with vancomycin were fabricated and evaluated in terms of their physical properties, delivery ability, and antimicrobial activity.</p><p><strong>Results: </strong>The MNs achieved a 378 μm depth of insertion into ex vivo skin and a 5.0 ± 0 mm zone of inhibition in agar disc diffusion assays. Furthermore, in ex vivo Franz cell experiments, the MNs delivered 34.46 ± 11.31 μg of vancomycin with around 35% efficiency, with 9.88 ± 0.57 μg deposited in the skin after 24 h.</p><p><strong>Conclusions: </strong>These findings suggest that sodium alginate MNs are a viable platform for antimicrobial agent delivery in SSTIs. Future in vivo studies are essential to confirm the safety and effectiveness of this innovative method for clinical use.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595082/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Surface-Immobilized States of Antimicrobial Peptides on Their Ability to Disrupt Bacterial Cell Membrane Structure.","authors":"Tong Lou, Xueqiang Zhuang, Jiangfan Chang, Yali Gao, Xiuqin Bai","doi":"10.3390/jfb15110315","DOIUrl":"10.3390/jfb15110315","url":null,"abstract":"<p><p>Antimicrobial peptide (AMP) surfaces are widely used to inhibit biofilm formation and bacterial infection. However, endpoint-immobilized AMPs on surfaces are totally different from free-state AMPs due to the constraints of the surface. In this work, the interactions between AMPs and bacterial cell membranes were analyzed through coarse-grained molecular dynamics and all-atom molecular dynamics simulations. This AMP disrupted membrane structure by altering the thickness and curvature of the membrane. Furthermore, the effect of surface-immobilized states of AMPs on their ability to disrupt membrane structure was revealed. The immobilized AMPs in the freeze-N system could bind to the membrane and disrupt the membrane structure through electrostatic forces between positively charged N-terminal amino acid residues and the negatively charged membrane, while the immobilized AMPs in the freeze-C system were repelled. The results will aid in the rational design of new AMP surfaces with enhanced efficacy and stability.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11595214/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetically Engineered Filamentous Bacteriophages Displaying TGF-β1 Promote Angiogenesis in 3D Microenvironments.","authors":"In-Hyuk Baek, Volkhard Helms, Youngjun Kim","doi":"10.3390/jfb15110314","DOIUrl":"10.3390/jfb15110314","url":null,"abstract":"<p><p>Combined 3D cell culture in vitro assays with microenvironment-mimicking systems are effective for cell-based screening tests of drug and chemical toxicity. Filamentous bacteriophages have diverse applications in material science, drug delivery, tissue engineering, energy, and biosensor development. Specifically, genetically modified bacteriophages have the potential to deliver therapeutic molecules or genes to targeted tumor tissues. The engineered bacteriophages in this study significantly enhanced endothelial cell migration and tube formation within the extracellular matrix (ECM). Compared to TGF-β1 alone and non-modified phages, the presence of TGF-β1 on the bacteriophages demonstrated superior performance as a continuous stimulant in the microenvironment, effectively promoting these angiogenic processes. Assays, including RT-qPCR, ELISA, and fluorescence microscopy, confirmed the expression of angiogenic markers such as CD31, validating the formation of 3D angiogenic structures. Our findings indicate that the TGF-β1 displayed by bacteriophages likely acted as a chemotactic factor, promoting the migration, proliferation, and tube formation of endothelial cells (ECs) within the ECM. Although direct contact between ECs and bacteriophages was not explicitly confirmed, the observed effects strongly suggest that TGF-β1-RGD bacteriophages contributed to the stimulation of angiogenic processes. The formation of angiogenic structures by ECs in the ECM was confirmed as three-dimensional and regulated by the surface treatment of microfluidic channels. These results suggest that biocompatible TGF-β1-displaying bacteriophages could continuously stimulate the microenvironment in vitro for angiogenesis models. Furthermore, we demonstrated that these functionalized bacteriophages have the potential to be utilized as versatile biomaterials in the field of biomedical engineering. Similar strategies could be applied to develop angiogenic matrices for tissue engineering in in vitro assays.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 11","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11594957/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142729515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pH-Responsive Ti-Based Local Drug Delivery System for Osteosarcoma Therapy.","authors":"Qinle Xiao, Changjun Wan, Zhe Zhang, Hui Liu, Pingting Liu, Qianli Huang, Dapeng Zhao","doi":"10.3390/jfb15100312","DOIUrl":"https://doi.org/10.3390/jfb15100312","url":null,"abstract":"<p><p>Osteosarcoma is one of the major bone cancers, especially for youngsters. The current treatment usually requires systemic chemotherapy and the removal of bone tumors. Titanium (Ti)-based implants can be modified as local drug delivery (LDD) systems for controllable and localized chemotherapeutic drug release. In this work, a pH-responsive Ti-based LDD prototype was designed by introducing polydopamine (PDA) to release doxorubicin (DOX) around osteosarcoma cells with low pH. Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and a contact angle meter were applied for surface characterization. Both direct and indirect cell culture modes were performed for biocompatibility and biofunction assessments. The results indicate that the Ti-based LDD prototype exhibits significant pH-dependent DOX release. The cumulative release can reach up to approximately 40% at pH = 6.0 after 72 h, but only around 20% at pH = 7.4. The Ti-based LDD implant shows good biocompatibility with approximately 93% viability of MC3T3 cells after direct culture in vitro for 24 h. Both direct and indirect culture modes verify the good anti-osteosarcoma function of the LDD implant, which should be attributed to the pH-responsive release of DOX.</p>","PeriodicalId":15767,"journal":{"name":"Journal of Functional Biomaterials","volume":"15 10","pages":""},"PeriodicalIF":5.0,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11508615/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}