Journal of ethnopharmacology最新文献

{"title":"Use and health effects of herbal medicine Ocotea guianensis during pregnancy in French Guiana","authors":"Gwladys Forsans ,&nbsp;Astrid Van-Melle ,&nbsp;Maelys Proquot ,&nbsp;Marc-Alexandre Tareau ,&nbsp;Jérôme Langrand ,&nbsp;Stéphanie Bernard ,&nbsp;Mathieu Nacher ,&nbsp;Guillaume Odonne ,&nbsp;Célia Basurko","doi":"10.1016/j.jep.2025.120161","DOIUrl":"10.1016/j.jep.2025.120161","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Ocotea guianensis</em> Aubl. leaves, in Creole <em>fey darjan</em> (“silver leaves”) are frequently cited by women in French Guiana for childbirth related practices. Although this use has been described in several ethnographic studies, the frequency of use of this plant has not been quantified yet. Moreover, beyond alarming anecdotes, the profile of users, the ways in which leaves are used during pregnancy, and its influence on childbirth and the immediate postpartum period remained to be determined. Our main objective was thus to estimate the frequency of <em>O. guianensis</em> use among women giving birth.</div></div><div><h3>Material and methods</h3><div>A descriptive cross-sectional study was conducted in the maternity wards of Cayenne and Kourou in the immediate postpartum period. Data were collected from 332 women between March 21 and July 10, 2023. Women willing to participate were invited by multilingual health workers to answer a 10-min structured quantitative questionnaire. Qualitative individual interviews were also conducted with some caregivers and patients.</div></div><div><h3>Results</h3><div>The frequency of use in the sample was 10 %. The leaves were generally used in the form of a sitz bath. Users were generally women born in French Guiana, with a relatively high level of education and social status. The study found no evidence of an increase in fetal distress or obstetrical complications in women who used <em>O.guianensis</em> compared with those who did not.</div></div><div><h3>Conclusion</h3><div>The study did not reveal any significant influence of <em>O. guianensis</em> use on pregnancy outcomes. Due to the small sample size of users and potential underreporting, this study may not have highlighted all user profiles in French Guiana. Even so, the study showed that the use of this herbal medicine concerned at least one in 10 women giving birth in Cayenne and Kourou, thus almost 484 deliveries per year. A complementary study conducted on a larger sample beyond maternity wards would refine usage frequency estimates and <em>O. guianensis</em> user profiles.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120161"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to Huanglian Jiedu decoction inhibits vascular smooth muscle cell-derived foam cell formation by activating autophagy via suppressing P2RY12 [J. Ethnopharmacol. 328 (2024) 118125. doi:10.1016/j.jep.2024.118125].","authors":"Jinhai Lin, Mingyang Gu, Xiaolong Wang, Yuanyuan Chen, Nhi Van Chau, Junlong Li, Qingmin Chu, Lijin Qing, Wei Wu","doi":"10.1016/j.jep.2025.120117","DOIUrl":"https://doi.org/10.1016/j.jep.2025.120117","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"120117"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144317068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zhen-wu-tang alleviates nephrotic syndrome by upregulating 5-HTR1B to activate AMPK/PGC-1α-mediated mitochondrial biogenesis","authors":"Beini Lao ,&nbsp;Xiaotong Lin ,&nbsp;Xipan Zhao ,&nbsp;Yuxian Li ,&nbsp;Xiuye Huang ,&nbsp;Yiwen Cao ,&nbsp;Shengliang Yuan ,&nbsp;Jiuyao Zhou","doi":"10.1016/j.jep.2025.120160","DOIUrl":"10.1016/j.jep.2025.120160","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Zhen-wu-tang (ZWT) as a classic herbal formula is widely recognized for its therapeutic efficacy in treating nephropathy. It is commonly used to alleviate edema, improve renal function, and manage symptoms associated with kidney yang deficiency. However, its therapeutic efficacy and mechanism for nephrotic syndrome (NS) is not yet clear.</div></div><div><h3>Aim of the study</h3><div>To investigate the underlying mechanism of ZWT in regulating mitochondrial biogenesis (MB) to treat NS.</div></div><div><h3>Methods</h3><div>An adriamycin (ADR)-induced NS animal model and a renal tubular epithelial cell injury model were used, followed by intervention with three concentrations of ZWT. The location of 5-HTR1B in kidney cells was observed using immunofluorescence co-localization. Protein levels of the MB-related proteins, including recombinant transcription factor A (TFAM), peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), adenosine 5′-monophosphate-activated protein kinase (AMPK) and p-AMPK, were determined by western blotting after administration of 5-hydroxytryptamine receptor 1B (5-HTR1B) agonist and inhibitor interventions. The bioactive compounds of ZWT-containing serum were determined using ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry.</div></div><div><h3>Results</h3><div>ZWT treatment ameliorated renal dysfunction and pathological damage in NS rats by attenuating podocyte and tubular cell damage. Additionally, ZWT reduced NS-induced mitochondrial dysfunction and reactive oxygen species levels. Immunofluorescence revealed that 5-HTR1B was predominantly expressed in the collecting ducts and proximal tubule cells. In ADR-induced models, ZWT upregulated the levels of PGC-1α, TFAM, AMPK and p-AMPK proteins involved in MB. In addition, a novel finding was that both gene and protein levels of 5-HTR1B were downregulated in these injury models, whereas ZWT intervention upregulated them. Studies on 5-HTR1B agonists and antagonists indicated that ZWT regulates AMPK/PGC-1α through 5-HTR1B-mediated mechanisms. Furthermore, 24 bioactive compounds were identified in ZWT-containing serum, of which paeoniflorin bound best to 5-HTR1B and acted similarly to 5-HTR1B agonists.</div></div><div><h3>Conclusion</h3><div>Overall, this study demonstrated that ZWT alleviates NS by upregulating MB via the 5-HTR1B/AMPK/PGC-1α signaling. This novel mechanism enriches the reference of ZWT for NS therapy.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"351 ","pages":"Article 120160"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144321345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gu Han Yang Sheng Jing improves major depressive disorder via attenuating neuroinflammation by inhibiting AKT1/p65 signaling pathway based on network pharmacology analysis and experimental validation","authors":"Xun Li ,&nbsp;San-Qiao Yang ,&nbsp;Hai-Jun Zhang ,&nbsp;Yuan Zhong ,&nbsp;Ge Wen ,&nbsp;Xuan Kang ,&nbsp;Zi-Jian Xiao","doi":"10.1016/j.jep.2025.120146","DOIUrl":"10.1016/j.jep.2025.120146","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Previous studies indicate that Gu Han Yang Sheng Jing (GHYSJ) exhibits neuroprotective properties in the central nervous system, but the protective effect of GHYSJ on major depressive disorder (MDD) and the precise mechanism underlying its protective effects remains unclear.</div></div><div><h3>Aim of the study</h3><div>This study aimed to clarify the antidepressant role of GHYSJ in MDD and the precise mechanism underlying via network pharmacology and experimental evaluations.</div></div><div><h3>Materials and methods</h3><div>Compounds-target-disease and protein-protein interaction network were constructed by network pharmacology to predict the potential targets of GHYSJ for the treatment of MDD. Molecular docking was used to predict the binding affinity between active components and pivotal targets. Protein expression levels were quantified via western blot analysis. The levels of proinflammatory factor were measured using ELISA kits. Immunofluorescence staining was performed to quantify Iba-1-positive cells. The antidepressant role of GHYSJ in MDD was tested by depression-like behavioral tests.</div></div><div><h3>Results</h3><div>This study identified a total of 94 active compounds and 185 targets of GHYSJ associated with MDD. Functional enrichment analyses revealed that inflammatory-related signalling pathways may be involved in GHYSJ-against MDD. Molecular docking demonstrated high-affinity binding between quercetin and TNF receptors. Furthermore, GHYSJ attenuated depressive-like behaviors of chronic unpredictable mild stress (CUMS)-exposed rats, reduced neuroinflammation, and inhibited the AKT1/p65 signaling pathway in the hippocampus of CUMS-exposed rats, which was reversed by the treatment of SC97 (AKT1 activator).</div></div><div><h3>Conclusion</h3><div>Taken together, These results suggest that GHYSJ improves MDD via attenuating neuroinflammation by inhibiting AKT1/p65 signaling pathway.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120146"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qing-Re-Xiao-Zheng-(Yi-Qi) formula attenuates the renal podocyte ferroptosis in diabetic kidney disease through AMPK pathway","authors":"Jiale Zhang ,&nbsp;Qiaoru Wu ,&nbsp;Chenhui Xia ,&nbsp;Huijuan Zheng ,&nbsp;Weimin Jiang ,&nbsp;Yaoxian Wang ,&nbsp;Weiwei Sun","doi":"10.1016/j.jep.2025.120157","DOIUrl":"10.1016/j.jep.2025.120157","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Diabetic kidney disease (DKD), a prevalent microvascular complication of diabetes, is a leading cause of end-stage renal disease (ESRD). Emerging evidence implicates ferroptosis in DKD pathogenesis. Qing-Re-Xiao-Zheng-(Yi-Qi) Formula (QRXZYQF), a traditional Chinese medicine with a 30-year clinical application history, exhibits multifaceted pharmacological benefits. But its potential role in DKD has yet to be fully investigated.</div></div><div><h3>Aim of the study</h3><div>This study investigates whether QRXZYQF alleviates podocyte injury and mitigates DKD progression by modulating ferroptosis through AMP-activated protein kinase (AMPK) pathway activation.</div></div><div><h3>Materials and methods</h3><div>We induced DKD in male sprague dawley (SD) rats by performing left unilateral nephrectomy followed by a single intraperitoneal injection of streptozotocin (STZ, 50 mg/kg). Rats received QRXZYQF (12/24 g/kg), metformin (100 mg/kg), and valsartan (8 mg/kg) for 16 weeks. Renal function, blood glucose, lipid profiles, 24-h urinary protein (24 h-UTP), oxidative stress markers glutathione (GSH) and malondialdehyde (MDA), and histopathology were assessed. In vitro, high-glucose-cultured conditionally immortalized mouse podocytes (MPC-5) cells were analyzed for cell viability assays, ferroptosis markers, mitochondrial integrity, and AMPK signaling. Additionally, we used short hairpin RNA (shRNA) to suppress AMPK expression to confirm whether QRXZYQF exerts protective effects on DKD via AMPK-mediated ferroptosis signaling.</div></div><div><h3>Results</h3><div>QRXZYQF improved body weight, glucose-lipid metabolism, and renal function in DKD rats, and alleviated kidney tissue pathology, renal fibrosi<strong>s</strong> and mitochondrial damage. Furthermore, QRXZYQF upregulated the expression of ferroptosis-related proteins glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) while downregulating acyl CoA synthase long-chain family member 4 (ACSL4) expression, and attenuated oxidative stress. Moreover, AMPK silencing partially reversed QRXZYQF's protective effects, confirming AMPK-dependent ferroptosis inhibition.</div></div><div><h3>Conclusions</h3><div>QRXZYQF attenuates DKD progression by activating AMPK signaling, thereby suppressing podocyte ferroptosis. These findings underscore its potential as a therapeutic agent for DKD.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"351 ","pages":"Article 120157"},"PeriodicalIF":4.8,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144306352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shaoyao Gancao decoction promotes splenic immune homeostasis mediating cGAS-STING signaling pathway to delay autoimmune hepatitis progression","authors":"Anli Xu ,&nbsp;Zequan Zhang ,&nbsp;Biran Zhu ,&nbsp;Min Xiao ,&nbsp;Xiaocui Jiang ,&nbsp;Yong Yang ,&nbsp;Min Zhao","doi":"10.1016/j.jep.2025.120121","DOIUrl":"10.1016/j.jep.2025.120121","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Shaoyao Gancao Decoction (SGD), a classic Chinese herbal formula from the “Treatise on Cold Damage (Shang Han Lun),” is widely used for treating liver disease. However, the efficacy and mechanisms by which SGD delays liver fibrosis progression in autoimmune hepatitis (AIH) require further investigation.</div><div><em>Aim of the study</em>: This study aimed to investigate the mechanisms underlying the SGD-mediated delay of liver fibrosis progression in AIH through the spleen.</div></div><div><h3>Materials and methods</h3><div>SGD was administered via gavage to mice for 15 days before establishing the concanavalin A (ConA)-induced AIH model. The key factors were identified using bioinformatics analysis. The intervention effects and mechanisms of SGD on AIH were clarified using liver function assay, enzyme-linked immunosorbent assay (ELISA), and histopathological, real-time quantitative PCR (RT-qPCR), immunofluorescence (IF), and Western blot analyses. Hepatic stellate cells (HSCs) were co-cultured with splenic mononuclear cells in a Transwell chamber system.</div></div><div><h3>Results</h3><div>SGD pretreatment alleviated liver injury, spleen injury, and abnormal liver function in AIH mice. Interleukin-1β (IL-1β) and IL-12 were identified as key factors in SGD intervention of AIH. SGD pretreatment promoted immune homeostasis in the spleen and liver. Additionally, SGD reduced HSC proliferation and activation. Further, SGD decreased cyclic GMP-AMP synthase (cGAS), stimulator of interferon genes (STING), nuclear factor kappa-B (NF-κB), and transforming growth factor-β1 (TGF-β1) expression in the liver and HSCs.</div></div><div><h3>Conclusions</h3><div>SGD promoted splenic immune homeostasis, which inhibited the hepatic cGAS-STING signaling pathway, thereby attenuating HSC activation and ultimately delaying liver fibrosis progression in AIH.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120121"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Standardization of Kushmanda Ghrita and Its Neuroprotective Effect in Trimethyltin-Induced Dementia in Wistar Rats: Insights into Cognitive Function and Oxidative Stress Modulation.","authors":"Swati R Dhande, Abhishek Gupta, Sampada Bhosale","doi":"10.1016/j.jep.2025.119976","DOIUrl":"https://doi.org/10.1016/j.jep.2025.119976","url":null,"abstract":"<p><p>An ethnopharmacological review reveals that Kushmanda ghrita is a polyherbal formulation made from Glycyrrhiza glabra L. (Yashtimadhu) and Benincasa hispida (Thunb.) Cogn. (Kushmanda) in ghrita (cow's ghee) and serves as a carrier for its active compounds. Yashtimadhu and Kushmanda have been reported to exhibit memory-enhancing and antioxidant activity.</p><p><strong>Aim of the study: </strong>This study aimed to standardize Kushmanda ghrita and evaluate its neuroprotective activity against trimethyltin-induced dementia in albino Wistar rats.</p><p><strong>Materials and methods: </strong>Kushmanda ghrita was standardized for various parameters according to the AYUSH protocol. The HPTLC method was developed and validated to standardize Kushmanda ghrita for the markers rutin, glycyrrhizin, gallic acid, and quercetin. Well-established parameters, such as locomotor activity, behavioral parameters (Morris water maze, elevated plus maze, social recognition test, and novel object recognition test), biochemical parameters (malonaldehyde, glutathione, and catalase), and histopathological studies were performed to evaluate the neuroprotective effects of trimethyltin-induced dementia in rats.</p><p><strong>Results and discussion: </strong>In addition to its organoleptic properties, Kushmanda ghrita was standardized for its specific gravity, viscosity, refractive index, loss on drying, pH, rancidity, iodine value, saponification value, acid value, peroxide value, heavy metal test (As, Cd, Hg, and Pb), microbial contamination test, and specific pathogen tests. A novel HPTLC method was developed to simultaneously detect and quantify selected markers (ICH Q₂ R₂ guidelines). Kushmanda ghrita treated groups significantly improved cognition and memory trimethyltin-induced dementia in rats. It also reduces malondialdehyde, glutathione, and catalase levels, thereby reducing oxidative stress in the brain. In addition, a brain histopathology study revealed less damage to neuronal structures in Kushmanda ghrita-treated animals than in diseased animals.</p><p><strong>Conclusion: </strong>Kushmanda ghrita was standardized, and an accurate, precise, robust, and specific HPTLC analytical method was established. Based on the pharmacological activity data, the study concludes that Kushmanda ghrita effectively reduces oxidative stress and prevents damage to hippocampal neurons, probably due to learning and memory enhancement activity.</p>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"119976"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sanleng Wan inhibits endometriosis progression by regulating sphingolipid metabolism via the S1P/S1PR1-Akt axis based on serum metabolomics and network pharmacology","authors":"Zhiyi Fu ,&nbsp;Hong Zhou ,&nbsp;Hao Liu ,&nbsp;Xiaoqiong Li ,&nbsp;Lixing Cao ,&nbsp;Weilin Zheng","doi":"10.1016/j.jep.2025.120153","DOIUrl":"10.1016/j.jep.2025.120153","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Endometriosis is a gynecological disorder characterized by chronic pelvic pain and infertility in women. The traditional herbal medicine Sanleng Wan (SLW) is composed of &lt;em&gt;Curcuma phaeocaulis&lt;/em&gt; Valeton (Chinese name: Ezhu) and &lt;em&gt;Sparganium stoloniferum&lt;/em&gt; (Buch.-Ham. ex Graebn.) Buch.-Ham. ex Juz. (Chinese name: Sanleng), has been recognized for its therapeutic potential in managing gynecological conditions, including endometriosis. In numerous traditional Chinese medicine prescriptions, SLW is classified as either the “principal drug” or the “minister drug.” However, the extent to which the therapeutic effects of SLW are mediated through the modulation of serum metabolites and associated molecular targets remains unclear.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;This study aimed to elucidate the potential mechanism by which SLW inhibits endometriosis, utilizing serum metabolomics, network pharmacology, molecular docking, SPR affinity analysis and preliminary &lt;em&gt;in vivo&lt;/em&gt; validation.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;A rat model of endometriosis was established using the autotransplantation method. The animals were divided into blank control, model, high-, middle-, and low-dose SLW groups, and the dienogest-treated group as a positive control. SLW or dienogest was administered for 4 weeks. Fibrosis in the lesions was assessed using Masson staining. Serum metabolomics analysis was conducted to investigate the effects of SLW on serum metabolites in endometriosis model rats, and metabolic pathways were analyzed using the MetaboAnalyst database. A metabolites-targets-pathways network was constructed using Cytoscape software. Additionally, surface plasmon resonance (SPR)-based binding affinity analysis was conducted to investigate potential direct interactions between the bioactive compounds and key proteins. Five major principal compounds (zederone, curcumin, ferulic acid, curcumol, and curdione) were evaluated for the binding capabilities with three proteins: sphingosine kinase 2 (SphK2), sphingosine-1-phosphate receptor 1 (S1PR1), and AKT serine/threonine kinase (Akt). Subsequently, the expression levels of transforming growth factor-β (TGF-β), sphingosine-1-phosphate (S1P), S1PR1, SphK2 and Akt, and phosphorylated Akt (p-Akt) in ectopic endometrium tissue were quantified using Western blot analysis.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;In a rat model, SLW inhibited the growth of endometriotic lesions and suppressed fibrosis. Metabolomic analysis revealed distinct serum metabolites associated with SLW treatment. A total of 76 significant metabolites were identified in the serum of the SLW-treated group, including L-threonine, (S)-homostachydrine, DL-arginine, and L-homoarginine, among others. These metabolites were involved in various metabolic pathways, including sphingolipid signaling, tyrosine metabolism, fatty acid degradation, arachidonic acid metabolism, pyruv","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"352 ","pages":"Article 120153"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Botanical repositories for Indian medicinal plants: A unified harmonisation approach to authentication, quality control, and conservation","authors":"Himanshu Sharma ,&nbsp;Sanjeev K. Lale ,&nbsp;Shyam B. Prasad ,&nbsp;J.C. Arya ,&nbsp;Vaibhav Charde ,&nbsp;Vijay Kumar ,&nbsp;Sujeet K. Mishra ,&nbsp;Hemant Soni ,&nbsp;Rajesh Bolleddu ,&nbsp;Sugreev K. Gaur ,&nbsp;Ch. Venkata Narasimhaji ,&nbsp;Nagayya Shiddamallayya ,&nbsp;Anupam K. Mangal ,&nbsp;Narayanam Srikanth ,&nbsp;Rabinarayan Acharya ,&nbsp;K.S. Dhiman ,&nbsp;Bidhan Mahajon ,&nbsp;Himanshu Dwivedi ,&nbsp;Mahesh K. Dadhich ,&nbsp;Rajeev K. Sharma ,&nbsp;S.N. Murthy","doi":"10.1016/j.jep.2025.120130","DOIUrl":"10.1016/j.jep.2025.120130","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>True botanical identity, quality issues, and conservation are the current gaps in the trade of Indian medicinal plant species used in traditional systems. The Ministry of AYUSH, India executed raw drug repositories (RDR) as a key strategy to solve such issues.</div><div><em>Aim of the study</em>:This review presents a unified concept of botanical repositories as a systematic approach to managing herbal raw drugs used in the Ayurveda, Siddha, and Unani (ASU) systems.</div></div><div><h3>Material</h3><div>Electronic databases, pharmacopoeias, reports, and books were referred to identify relevant literature.</div></div><div><h3>Results</h3><div>The review systematically organizes scientific literature of botanical repositories in a concise and structured manner, making it accessible and comprehensible. It proposed a networking of the AYUSH RDR with a special focus on the National Raw Drug Repository (NRDR) with existing herbal repositories to explore future opportunities in the AYUSH domain. The study categorised raw drugs, their profiling, and the methodology for development of botanical reference substances (BRS). It discussed the significance of NRDR in terms of BRS, harmonisation of herbal trade, and botanical research. This work proposed a schematic design for a virtual repository to enhance user accessibility.</div></div><div><h3>Conclusion</h3><div>The study serves as a guide for researchers, stakeholders, and practitioners to resolve issues on Indian medicinal plant species. It will raise awareness about the collection center of ASU drugs, authentication facilities, BRS, and proposed a sustainability plan &amp; recommendations for betterment of RDR. The study serves as a role model for their further extension into other Indian geographical zones.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"351 ","pages":"Article 120130"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the detoxification components and mechanisms of glycyrrhizae radix et rhizoma: Traditional and modern clinical perspectives","authors":"Jie Dong ,&nbsp;Gang Tian ,&nbsp;Qiongfang Xu ,&nbsp;Wenli Jiang ,&nbsp;Ruina Liu ,&nbsp;Jingqi Han ,&nbsp;Yu Xiong ,&nbsp;Bo Zhu ,&nbsp;Qiyuan Shan ,&nbsp;Gang Cao","doi":"10.1016/j.jep.2025.120154","DOIUrl":"10.1016/j.jep.2025.120154","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Glycyrrhizae Radix et Rhizoma (GRR), is a widely used herbal medicines in traditional Chinese medicine (TCM) known for its detoxification properties. GRR plays a significant role in reducing the toxic effects of harmful substances and drugs in both TCM and Western medicine.</div></div><div><h3>Aim of the study</h3><div>This review investigates the detoxification components and mechanisms of GRR from both traditional and modern clinical perspectives.</div></div><div><h3>Materials and methods</h3><div>Literature published or earlier accessed up to December 31, 2024 was searched from PubMed, Web of Science and CNKI databases. Keywords “licorice detoxification”, “licorice processing”, “licorice compatibility”, etc., were used for screening. In addition, information from TCM classic texts was collected.</div></div><div><h3>Results</h3><div>GRR's ability to harmonize medicinal properties and reduce toxicity is supported by both historical usage and emerging pharmacological evidence. In TCM, the sweet flavor and neutral nature of GRR are the key points to processed or prescribed with other herbs for detoxification and harmonization. From modern pharmacological view, the extracts of GRR and its bioactive components, exhibit significant detoxification effects both <em>in vivo</em> and <em>in vitro</em>. The detoxification of GRR primarily involve physical and chemical detoxification mechanisms, and biological detoxification by pharmacokinetics (PK) and pharmacodynamics (PD) modulations via gut microbiota, phase I-III metabolism, as well as immune regulations.</div></div><div><h3>Conclusion</h3><div>By integrating classical theory with contemporary scientific evidence, we seek to advance the understanding of GRR's role in harmonizing medicinal properties and mitigating the toxicity of various compounds, to provide a theoretical foundation and insights for the further development and pharmacological utilization of GRR.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"351 ","pages":"Article 120154"},"PeriodicalIF":4.8,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}