Journal of ethnopharmacology最新文献

{"title":"Saorilao Qingfei Zhike Capsule alleviated inflammation and bronchial remodeling in LPS and CS-induced COPD via MAPK signaling pathway","authors":"Yanan Wang ,&nbsp;Fengyu Han ,&nbsp;Lisha Ye ,&nbsp;Tongqiang ShangGuan ,&nbsp;Shengci Fan ,&nbsp;Yihuan She ,&nbsp;Xiuling Yu ,&nbsp;Dongmei Wang ,&nbsp;Baolian Wang","doi":"10.1016/j.jep.2025.119993","DOIUrl":"10.1016/j.jep.2025.119993","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Chronic obstructive pulmonary disease (COPD) is a persistent inflammatory airway disease primarily caused by prolonged exposure to toxic gases or particles. The Saorilao Qingfei Zhike Capsule (SRL), derived from a traditional Mongolian medicine recipe, has demonstrated promising clinical efficacy in treating chronic bronchitis. However, its potential role in COPD treatment remains unclear.</div></div><div><h3>Aim of the study</h3><div>This study aimed to assess the potential of SRL in treating COPD and further related mechanisms by utilizing network pharmacology and experimental validation.</div></div><div><h3>Materials and methods</h3><div>The study induced COPD in mice through intratracheal instillation of LPS combined with exposure to CS. From day 57 to day 84, the mice were treated with distilled water, NAC, or SRL at doses of 200, 400, and 800 mg/kg. Lung function indices and histopathological examinations were used to evaluate the therapeutic efficacy of the treatments. Furthermore, network pharmacology, along with <em>in vitro</em> and <em>in vivo</em> experiments, was conducted to explore the potential mechanisms by which SRL against COPD.</div></div><div><h3>Results</h3><div>The study demonstrated that SRL improved lung function and reduced lung index in COPD mice. Histopathological analysis revealed that SRL decreased apoptosis, collagen accumulation, airway inflammation, and excessive mucus production, highlighting its therapeutic potential in COPD treatment. RT-PCR analysis showed a marked decrease in the expression of Pro-Col I, Pro-Col III, TIMP-1, and TIMP-2 in SRL-treated COPD mice. Furthermore, Western blot analysis indicated a substantial increase in the expression levels of p-Smad3, p-P38 and p-JNK in the lung tissue of COPD mice compared to normal controls, suggesting activation of the MAPK pathway, which was consistent with network pharmacology predictions. Additionally, SRL treatment obviously suppressed the phosphorylation of these proteins and significantly reduced α-SMA expression.</div></div><div><h3>Conclusion</h3><div>It was firstly reported that SRL exhibited promising therapeutic potential for treating COPD, possibly through the activation of the MAPK pathway.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"350 ","pages":"Article 119993"},"PeriodicalIF":4.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heshuxiaoji pill suppresses steatohepatitis and fibrosis by regulating the AngII-BACH1 mediated vasoconstriction","authors":"Yueheng Pu ,&nbsp;Wei Ren ,&nbsp;Zhonghua Gan ,&nbsp;Shiyang Wang ,&nbsp;Mengyun Peng ,&nbsp;Rensong Yue ,&nbsp;Rui Huang","doi":"10.1016/j.jep.2025.119989","DOIUrl":"10.1016/j.jep.2025.119989","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Non-alcoholic steatohepatitis (NASH), a widespread hepatic affliction marked by hepatic fibrosis progression towards hepatocellular carcinoma, is significantly influenced by endothelial dysfunction and endothelial-to-mesenchymal transition (EndMT). Although Heshuxiaoji (HSXJ) Pill, an empirical prescription formulated by Prof. Tongjiao Sun has demonstrated significant efficacy in mitigating steatohepatitis and fibrosis, the precise mechanisms underlying its therapeutic effects remain to be fully elucidated.</div></div><div><h3>Aim of the study</h3><div>To investigate the antifibrotic effect of HSXJ pill and to explore its mechanism in vivo and in vitro.</div></div><div><h3>Materials and methods</h3><div>To probe the antifibrotic impact of HSXJ pill and unravel its mechanisms, murine liver fibrosis and NASH models were induced in vivo via Western diet and CCl₄ injection. In vitro, human umbilical vein endothelial cells were stimulated with AngII, followed by Western blot analysis. Additionally, liver biopsies from patients with mild-to-moderate fibrosis (S1–S2) were utilized to verify EndMT involvement in fibrosis.</div></div><div><h3>Results</h3><div>In the hepatocyte sections exhibiting human liver fibrosis, we observed a significant upregulation of AngII and the transcription factor BTB and CNC homology 1 (BACH1). Genetic ablation of AngII significantly ameliorates hepatic fibrosis and EndMT, while attenuating pathological angiogenesis via decreased BACH1 expression. In contrast, AngII overexpression exacerbates these conditions. In vivo, the HSXJ pill effectively alleviates hepatic fibrosis, reduces alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, and suppresses BACH1 and AngII production, thereby inhibiting EndMT. In vitro, the pill mitigates EndMT-associated fibrosis by regulating BACH1 to inhibit AngII activation.</div></div><div><h3>Conclusion</h3><div>The study indicates that the HSXJ pill effectively diminishes hepatocyte injury markers and alleviates liver fibrosis, with optimal efficacy at medium/high doses. BACH1 serves as a key regulator of hepatic fibrosis via modulation of AngII expression.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"350 ","pages":"Article 119989"},"PeriodicalIF":4.8,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic effects of Saussurea graminea Dunn and its active compounds in sepsis-associated liver injury: Transcriptomics, metabolomics and experimental validation","authors":"Yushun Cui ,&nbsp;Zhiqiang Li ,&nbsp;Miao Lai ,&nbsp;Ying Yang ,&nbsp;Zhengwen Zhang ,&nbsp;Yulin Feng ,&nbsp;Min Yao ,&nbsp;Junmao Li","doi":"10.1016/j.jep.2025.119985","DOIUrl":"10.1016/j.jep.2025.119985","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Saussurea graminea</em> Dunn (SG), a traditional Chinese medicinal herb known as \"Za Chi\" in Tibet of China, is frequently utilized in the treatment of inflammatory diseases such as hepatitis. However, the active ingredients and mechanism of its therapeutic effect on Sepsis - associated liver injury (SALI) remain unclear.</div></div><div><h3>Aim of the study</h3><div>To elucidate the effect of SG in combating SALI, uncover its mechanism of action, and explore possible active compounds.</div></div><div><h3>Materials and methods</h3><div>We established a SALI model by intraperitoneal injection of lipopolysaccharide to assess the efficacy of SG. Transcriptomics and metabolomics were employed to reveal its possible mechanism of action. Subsequently, Western blot, flow cytometry, confocal microscopy, quantitative PCR, HPLC-MS, and molecular docking were utilized to verify its mechanism and active ingredients.</div></div><div><h3>Results</h3><div>SG effectively counteracts SALI by inhibiting the cytokine storm. Transcriptomics indicates that SG regulates SALI through mitochondrial/TNF and metabolic pathways. Metabolomics demonstrates that arachidonic acid metabolism is involved in the process of SG treating SALI. HPLC-MS identified the main components of SG as chlorogenic acid, syringin, scopoletin, rutin, isochlorogenic acid, and narcissin, and these six compounds were confirmed as potential active components in the RAW264.7 inflammation model.</div></div><div><h3>Conclusion</h3><div>SG and its active ingredients play a role in alleviating SALI by reducing the cytokine storm through mtDNA/TNF/arachidonic acid metabolism.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119985"},"PeriodicalIF":4.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Smilax china L. Rhizome extract enhances anti-tumor immune responses by resetting M2-like macrophages and tumor-associated macrophages to M1-like via ERK1/2 signaling","authors":"Yingxue Guo ,&nbsp;Xiaochen Lin ,&nbsp;Penghao Wang ,&nbsp;Yingying Wang ,&nbsp;Mengyun Chen ,&nbsp;Shuiyan Tang ,&nbsp;Lu Jin ,&nbsp;Weiye Mao ,&nbsp;Xia Liu ,&nbsp;Qiyang Shou ,&nbsp;Huiying Fu","doi":"10.1016/j.jep.2025.119983","DOIUrl":"10.1016/j.jep.2025.119983","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Sm<em>ilax china</em> L. is a traditional Chinese herb. <em>Smilax china</em> L. Rhizome (SCR) have historically been used in ethnomedicine for their anti-inflammatory, anti-tumor, and immunomodulatory properties.</div></div><div><h3>Aim of the study</h3><div>This study aimed to evaluate the anti-tumor efficacy of SCR in the MMTV-PyMT mouse mammary tumor model and elucidate its immunomodulatory mechanisms within the tumor microenvironment (TME).</div></div><div><h3>Materials and methods</h3><div>SCR was administered to MMTV-PyMT mice to assess its effects on tumor progression and metastasis. Immune cell profiling (M1/M2-like macrophages, CD8⁺ T cells) was conducted via flow cytometry. <em>In vitro</em> macrophage polarization assays under IL-4 stimulation and mechanistic studies (MAPK/ERK signaling) were performed using Western blot and pharmacological inhibitors. Diosgenin, a key SCR constituent, was identified and validated through phytochemical analysis and functional assays.</div></div><div><h3>Results</h3><div>SCR treatment significantly slowed primary tumor growth and reduced lung metastases. SCR induces a shift in macrophage polarization from immunosuppressive M2-like to proinflammatory M1-like and promotes increased CD8⁺ T cell infiltration. <em>In vitro</em>, SCR inhibited IL-4-induced M2 polarization and suppressed ERK1/2 phosphorylation, a critical node in the MAPK pathway. Diosgenin was identified as a pivotal bioactive compound contributing to SCR's anti-tumor and immunomodulatory effects.</div></div><div><h3>Conclusions</h3><div>These findings provide a theoretical basis for the potential clinical application of SCR in cancer treatment, highlighting its critical role in remodeling the tumor immune microenvironment.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119983"},"PeriodicalIF":4.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new incompatible combination: Reynoutria multiflora combined with Cullen corylifolium enhances idiosyncratic hepatotoxicity under immunological stress","authors":"Li Lin ,&nbsp;Longxin Guo ,&nbsp;Minjuan Long ,&nbsp;Ming Niu ,&nbsp;Yuming Guo ,&nbsp;Shengkai Zhu ,&nbsp;Zhaofang Bai ,&nbsp;Xu Zhao ,&nbsp;Huaqiang Zhai ,&nbsp;Xiaohe Xiao","doi":"10.1016/j.jep.2025.119986","DOIUrl":"10.1016/j.jep.2025.119986","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Compound preparations of traditional Chinese medicines (TCMs) have gained considerable interest. However, ensuring their safety is difficult because of ingredient complexity. &lt;em&gt;Reynoutria multiflora&lt;/em&gt; (Thunb.) Moldenke (PM), commonly used in tonic preparations for clinical use, has been frequently reported to cause drug-induced liver injury (DILI). Liver injury caused by PM is idiosyncratic and its underlying mechanisms have been elucidated. However, a systematic evaluation of the safety of PM compound formulations is lacking. Our preliminary research predicted a potential compatibility risk between PM and &lt;em&gt;Cullen corylifolium&lt;/em&gt; (Linnaeus) Medikus (PF); however, the specific effects and mechanisms of their combined action on the liver have not been fully studied.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;To determine the effects of the PM and PF combination on the liver and its mechanisms.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;This study used a lipopolysaccharide-induced idiosyncratic (IDILI) model. Kits were employed to measure the liver function indices and ELISA was used to detect inflammatory factor content. The histopathological changes of the liver were observed using H&amp;E and TUNEL staining. Serum metabolomics and liver transcriptomics were performed simultaneously to detect overall differences in metabolite and gene expression. Network pharmacology and molecular docking were used to screen hepatotoxic components and their potential pathways.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Liver function indicators and pathological results demonstrated that the combined treatment with PM and PF may exacerbate immune-mediated liver injury compared to treatment with either herb alone. Inflammatory factor levels indicated that the combination had an immuno-amplifying effect, further increasing inflammatory cytokine levels. Integrated metabolomic and transcriptomic analysis revealed that the combination primarily affected the primary bile acid synthesis and glycerophospholipid metabolism. This exacerbated hepatocyte apoptosis and liver injury by down-regulating the expression of key genes (&lt;em&gt;BAAT&lt;/em&gt; and &lt;em&gt;ALB&lt;/em&gt;) for bile acid metabolism. Network pharmacology and molecular docking identified 2,3,5,4′-Tetrahydroxy stilbene-2-Ο-β-D-glucoside and psoralidin as potential toxic components of PM and PF, respectively. These two ingredients specifically targeted the cytosolic DNA-sensing pathway and NOD-like receptor signalling pathway. When combined, PM and PF triggered immune hyperactivation via dual-pathway cross-regulation, leading to immuno-metabolic disorders, increased hepatocyte apoptosis and enhanced inflammatory cascade responses.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;The PM and PF combination represents a newly identified incompatible pair that can aggravate IDILI under specific conditions. Our findings provide a critical reference for the safe use of compound m","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"350 ","pages":"Article 119986"},"PeriodicalIF":4.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Mass spectrometry-based metabolomics reveal Dendrobium huoshanense polysaccharide effects and potential mechanism of N-methyl-N'-nitro-N-nitrosoguanidine -induced damage in GES-1 cells\" [J. Ethnopharmacol. 310 (2023) 116342].","authors":"Huiqun Xie, Mengqing Hu, Jiao Yu, Xinyu Yang, Jinmiao Li, Nianjun Yu, Lan Han, Daiyin Peng","doi":"10.1016/j.jep.2025.119927","DOIUrl":"https://doi.org/10.1016/j.jep.2025.119927","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"119927"},"PeriodicalIF":4.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LC-MS/MS profiling of Zanthoxylum piperitum (L.) DC. leaves cultivated in Egypt, isolation of its bioactive components, and interrelationships with anti-ulcerative activities: in vitro and in vivo approaches, molecular docking, and dynamics studies","authors":"Nermin S. El Tayeb ,&nbsp;Nermin A. Younis ,&nbsp;Samar M. Mouneir ,&nbsp;Kawkab A. Ahmed ,&nbsp;Ahmed A. Al‐Karmalawy ,&nbsp;Radwan Alnajjar ,&nbsp;Azza R. Abdel-monem ,&nbsp;Nesrin M. Fayek","doi":"10.1016/j.jep.2025.119984","DOIUrl":"10.1016/j.jep.2025.119984","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div><em>Zanthoxylum piperitum</em> (L.) DC. cultivated in Egypt is a new cultivar of the known anti-inflammatory food spice <em>Zanthoxylum piperitum</em> (L.) DC.</div></div><div><h3>Aim of the study</h3><div>This study aimed to investigate the new cultivar leaves phytochemically and biologically.</div></div><div><h3>Materials and methods</h3><div>UPLC-Triple TOF-MS/MS analysis examined the ethanolic extract's composition, and FRAP and ORAC assays evaluated its antioxidant activity. Major flavonoids were isolated and identified by spectroscopic techniques. The anti-inflammatory properties of the extract and isolated compounds were investigated <em>in vitro.</em> Then, by <em>in vivo</em> acetic acid-induced UC model on Sixty-three male Wistar rats at high and low doses compared to standard prednisolone. Evaluation involved macroscopic and microscopic assessment of rectal damage, cytokine measurement, molecular docking, and dynamic simulations to support the findings.</div></div><div><h3>Results</h3><div>Extract of <em>Zanthoxylum piperitum</em> (L.) DC. Cultivated in Egypt demonstrated rich phenolic content and antioxidant action. Isoquercitrin and quercitrin were isolated and identified via spectroscopy. The <em>in vitro</em> anti-inflammatory tests showed that the extract inhibited COX1 and LOX significantly (<em>p &lt;</em> 0.05). In the <em>in vivo</em> UC model, all treatments ameliorated the macroscopic and microscopic damage in the intestine of UC rats' and improved the biochemical markers in a dose-dependent manner, especially isoquercitrin at a high dose (40 mg/kg) showed the most significant results (<em>p &lt;</em> 0.05) compared to the standard group. Molecular docking and dynamics studies supported these findings.</div></div><div><h3>Conclusions</h3><div>This indicates the potential of the extract of <em>Zanthoxylum piperitum</em> (L.) DC. cultivated in Egypt and its main constituents for the treatment of UC.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"350 ","pages":"Article 119984"},"PeriodicalIF":4.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cistanche deserticola polysaccharides protect against cyclophosphamide-induced premature ovarian failure in mice by regulating the JAK-STAT pathway","authors":"Jinhua Li ,&nbsp;Weiwei Sun ,&nbsp;Xiuli Wan ,&nbsp;Yanping Zhang ,&nbsp;Xitang Yang ,&nbsp;Bangyu Ouyang ,&nbsp;Qing Zhang ,&nbsp;Qian Wang ,&nbsp;Xue Li ,&nbsp;Xinrui Liu ,&nbsp;Yikai Qiu ,&nbsp;Xiaoli Yu ,&nbsp;Xiuying Pei","doi":"10.1016/j.jep.2025.119971","DOIUrl":"10.1016/j.jep.2025.119971","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Classical Chinese medical texts, notably the Compendium of Materia Medica (Ben Cao Gang Mu) and Zhong Hua Ben Cao (Chinese Herbal Medicine), have historically documented <em>Cistanche deserticola's</em> application as a tonic herb for addressing reproductive disorders including male impotence, reduced fertility and female menstrual infertility, among other conditions. Polysaccharides from <em>Cistanche deserticola</em> are recognized as the plant's principal bioactive components. Nevertheless, the therapeutic potential and mechanistic actions of <em>Cistanche deserticola</em> polysaccharides (CDPs) in premature ovarian failure (POF) remain unexplored.</div></div><div><h3>Aim of the study</h3><div>This study aimed to determine the protective role of CDPs in POF and to elucidate the underlying mechanisms.</div></div><div><h3>Material and methods</h3><div>POF mouse models were developed through intraperitoneal administration of cyclophosphamide (CTX) at a high dose of 120 mg/kg/day and followed by 8 mg/kg/day (low maintenance dose) administered daily for 14 consecutive days. In a prophylactic therapeutic regimen, CDPs received pre-treatment initiation two weeks before model establishment, with phased administration maintained throughout three distinct temporal parameters (2-, 6-, and 8-week intervals) during pharmacological intervention. Upon anesthetization of the mice, ovarian tissues were collected for subsequent histopathological and molecular investigations. These analyses included immunohistochemistry to detect apoptotic proteins, Proliferative index mapping through Ki67 immunofluorescence, and electron microscopy to assess mitochondrial status and other pertinent indicators. RNA-seq elucidated the core regulatory pathway governing POF and potential protective targets for CDPs.</div></div><div><h3>Results</h3><div>Experimental evidence established that intragastric administration of CDPs ameliorate histopathological ovarian lesions and rescue endocrine homeostasis, thereby enhancing the health of offspring in CTX-induced POF mice. This effect is facilitated by the promotion of follicular development, the proliferation of follicles, and the suppression of granulosa cell apoptosis. Furthermore, CDPs significantly attenuation of oxidative stress via ROS scavenging and restore mitochondrial morphology and function. In conclusion, the protective role of CDPs are closely linked to the JAK-STAT signaling pathway in POF models.</div></div><div><h3>Conclusion</h3><div>Our findings demonstrate that CDPs are capable of protecting protect the ovary tissue against CTX-induced damages through suppression of the activation of the JAK-STAT pathway and attenuation of granulosa cells (GCs) apoptosis.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119971"},"PeriodicalIF":4.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological effects and mechanisms of alkamides DDA-E and DDA-Z from Asari Radix et Rhizoma in migraine: Insights from serum pharmacochemistry, network pharmacology, and experimental validation","authors":"Fujie Cai ,&nbsp;Hanxue Wang ,&nbsp;Hanze Liu ,&nbsp;Wenkang Liu ,&nbsp;Xianrun Hu ,&nbsp;Sitong Zhang ,&nbsp;Junyi Wang ,&nbsp;Min Zheng ,&nbsp;Rui Dang ,&nbsp;Mireyi Bahatijiang ,&nbsp;Huida Guan ,&nbsp;Xuemei Cheng ,&nbsp;Changhong Wang","doi":"10.1016/j.jep.2025.119978","DOIUrl":"10.1016/j.jep.2025.119978","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Asari Radix et Rhizoma (ARR), a traditional Chinese medicine, has been used for centuries to treat various diseases, including migraine, rheumatic pain, and toothache. Valued for its capacity to warm the meridians and promote dispersion, ARR is regarded as an essential herb for releasing the exterior, dispelling cold, and alleviating pain. Alkamides, represented by &lt;em&gt;N&lt;/em&gt;-isobutyl-2&lt;em&gt;E&lt;/em&gt;,4&lt;em&gt;E&lt;/em&gt;,8&lt;em&gt;Z&lt;/em&gt;,10&lt;em&gt;E/Z&lt;/em&gt;-dodecatetraenamide (DDA-E/Z), were considered closely related to traditional properties of ARR and exhibited diverse biological activities. However, their underlying anti-migraine mechanisms remain unclear.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;This study aimed to identify the active constituents of ARR and investigate the pharmacological effects and mechanisms of ARR-derived alkamides DDA-E and DDA-Z in migraine models through integrated network pharmacology and experimental validation.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Material and methods&lt;/h3&gt;&lt;div&gt;The chemical profile and blood entry components of ARR were analyzed by ultra-high performance liquid chromatography-quadruple time-of-flight mass spectrometry (UHPLC-Q/TOF-MS). Network pharmacology was used to identify potential targets and pathways associated with the prototypical plasma components in migraine. Analgesic and anti-inflammatory activities of the key active ingredients, DDA-E, DDA-Z and their combinations, were assessed using behavioral tests and periorbital mechanical pain thresholds. Hematoxylin and eosin, immunofluorescence analysis, enzyme-linked immunosorbent assay, Westen blot, and real-time quantitative reverse transcription PCR were conducted to explore underlying mechanisms. Functional assay and molecular docking studies investigated the ability of DDA-E and DDA-Z to activate CB1/CB2 receptors.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;A total of 35 components were identified in ARR, with 10 of them entering the blood as prototypes. Network pharmacology revealed 209 potential targets of ARR-derived prototypical blood-entry components in migraine. DDA-E and DDA-Z showed high plasma exposure and the highest degree values in the network analysis, indicating their roles as important active ingredients of ARR for migraine. The &lt;em&gt;in vitro&lt;/em&gt; and &lt;em&gt;in vivo&lt;/em&gt; experiments suggested that the potential targets of DDA-E were CGRP, ERK, and AKT, which mainly acted through cAMP and PI3K-Akt pathways, while DDA-Z targeted COX-2, MAPK, and AKT through MAPK and PI3K-Akt pathways. Immunofluorescence, functional assays, and molecular docking results confirmed that DDA-E and DDA-Z tend to selectively activate CB1/CB2 receptors.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;In this study, serum pharmacochemistry combined with network pharmacology identified DDA-E and DDA-Z as the key active constituents of ARR. Subsequent experimental validation elucidated their potential anti-migraine mechanisms, highlighting their selective","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119978"},"PeriodicalIF":4.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Shuangyang Houbitong granules against acute erythroleukemia through PI3K/AKT and JAK/STAT signaling pathways","authors":"Xiang Liu ,&nbsp;Bo Wang ,&nbsp;Yu Mou ,&nbsp;Yulin Sun ,&nbsp;Qing Rao ,&nbsp;Jingrui Song ,&nbsp;Yubing Huang ,&nbsp;Min Wen ,&nbsp;Lei Huang ,&nbsp;Yanmei Li","doi":"10.1016/j.jep.2025.119979","DOIUrl":"10.1016/j.jep.2025.119979","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Traditional Chinese Medicine (TCM) posits that the onset of leukemia is often attributed to internal deficiencies and imbalances between yin and yang. Shuangyang Houbitong granules (SY) have been used in folk medicine to treat leukemia by balancing the yin-yang. However, the molecular mechanisms in which SY exerts anti-leukemia effects remain largely unexplored.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;To investigate the molecular mechanisms and bioactive ingredients of SY for the treatment of acute erythroleukemia (AEL).&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Materials and methods&lt;/h3&gt;&lt;div&gt;A mouse model of erythroleukemia was established using Friend murine leukemia virus (F-MuLV). The therapeutic effect of SY in erythroleukemic mice was assessed through various methods, including examining spleen morphology, measuring biochemical parameters, evaluating erythrocyte differentiation, analyzing splenic immune cell markers, conducting histopathological staining, and recording survival rates. Erythroleukemia cell viability and apoptosis were measured using MTT assays, flow cytometry, and JC-1 staining. Furthermore, network pharmacology and RNA sequencing (RNA-seq) were employed to identify differentially expressed genes and explore regulatory pathways. Western blotting was utilized to detect relevant protein expression, and the components of SY were analyzed using UHPLC-Q-TOF/MS.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The &lt;em&gt;vivo&lt;/em&gt; experiments revealed that SY significantly repaired F-MuLV-induced splenic injuries in erythroleukemic mice. Flow cytometry analysis indicated that SY promoted differentiation towards mature erythrocytes, markedly reducing the ratio of CD71/Ter119 in the spleens of the treated mice. In addition, SY enhanced the proportion of immune cells (CD3, CD4, CD8a, B220, and CD11b), effectively activating the immune system and prolonging the survival of the mice. &lt;em&gt;In vitro&lt;/em&gt;, SY significantly induced apoptosis in human erythroleukemia (HEL) cells in a time- and dose-dependent manner, inhibiting HEL cell proliferation. The results of network pharmacology and RNA sequencing suggested that SY may inhibit HEL cell proliferation by affecting the PI3K/AKT, JAK/STAT signaling pathways. Consistent with the above findings, SY increased the expression of apoptosis-related proteins (Bad, cleaved caspase-3/9, and cleaved PARP) and key proteins of the JAK/STAT signaling pathway (p-JAK2, p-STAT3), while down-regulating Bcl-xl and proteins related to the PI3K/AKT signaling pathway (p-PI3K and p-AKT) in the HEL cells. Using UHPLC-Q-TOF/MS analysis, a total of 144 compounds were identified in the component of SY that entered the serum. We evaluated the anti-erythroleukemia activity of eight selected compounds (luteolin, genistin, isofraxidin, glabridin, isovitexin, bergenin, diosmetin, and xanthohumol) from the initial 144 compounds. Notably, xanthohumol (XAN) exhibited the most significant inhibition of HEL","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"350 ","pages":"Article 119979"},"PeriodicalIF":4.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}