Journal of ethnopharmacology最新文献

{"title":"Yinchenhao Tang exerts an inhibitory effect on the influenza a virus by modulating the JAK/STAT signaling pathway","authors":"Yeqing Lu ,&nbsp;Yu Huang ,&nbsp;Qiaoli Lu ,&nbsp;Liting Fu ,&nbsp;Mengjie Xiao ,&nbsp;Pei Feng ,&nbsp;Huihuang Deng ,&nbsp;Run-Feng Li ,&nbsp;Huihui Ti","doi":"10.1016/j.jep.2025.119886","DOIUrl":"10.1016/j.jep.2025.119886","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Yinchenhao Tang (YCHT), a traditional Chinese medicine (TCM), consists of Yinchen, Zhizi, and Dahuang. For over a millennium, it has been widely used in the treatment of liver disorders and jaundice. Furthermore, numerous studies have highlighted its anti-inflammatory, immunomodulatory, and antiviral properties. However, the specific active constituents and the involvement response to influenza virus infection remain unclear.</div></div><div><h3>Aim of the study</h3><div>The present study aims to explore the YCHT’ s antiviral and anti-inflammatory abilities, and further clarify the possible mechanisms underlying such effects.</div></div><div><h3>Materials and methods</h3><div><em>In vitro</em> and <em>in vivo</em> studies were performed to confirm the anti-influenza and anti-inflammatory abilities of YCHT. Ultra-Performance Liquid Chromatography-Quadrupole Orbitrap High-Resolution Mass Spectrometry (UPLC-Q-Orbitrap HRMS) analysis was used to determine the active components and mechanism of YCHT. The potential mechanisms exert its anti-influenza virus effects were investigated <em>via</em> network pharmacology and molecular docking, and the results of cell cytokines and signaling pathways were elucidated by Western blot.</div></div><div><h3>Results</h3><div>The findings showed that YCHT increased the survival rate of virus-infected mice, reduced viral titers in lung tissue and improved lung pathology. Moreover, in viral-infected A549 cells and pulmonary tissues, inflammatory cytokine expression was suppressed. A total of 26 active compounds were identified in the serum of infected mice in the YCHT treatment group. According to experimental research, molecular docking, and network pharmacology, YCHT inhibits the JAK/STAT signaling pathway to provide its anti-influenza effects.</div></div><div><h3>Conclusions</h3><div>This study verified YCHT could prevent the production of inflammatory cytokines after influenza A virus infection and relieve the pneumonia <em>via</em> the regulation of JAK/STAT signaling pathway.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119886"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whether traditional Chinese medicine injection can reduce adverse events in patients with cancer? A meta-analysis of randomized controlled trials","authors":"Zilin Long ,&nbsp;Houyu Zhao ,&nbsp;Fengqi Liu ,&nbsp;Meng Zhang ,&nbsp;Feng Sun","doi":"10.1016/j.jep.2025.119969","DOIUrl":"10.1016/j.jep.2025.119969","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Adverse events of anticancer treatment were common and debilitating in cancer patients. Traditional Chinese medicine injection (TCMI) plays an important role in the comprehensive treatment of cancer in China.</div></div><div><h3>Aim of the review</h3><div>To explore whether TCMI can reduce adverse events of anticancer treatment in patients with cancer.</div></div><div><h3>Materials and methods</h3><div>Ten databases (i.e., Embase, Web of Science, PubMed, ClinicalTrials.gov, the Cochrane Library, CBM, Scoups, CNKI, Wangfang Database and VIP) were searched up to July 2, 2024. RCT was included if it assessed TCMI in cancer patients and reported any type of adverse events. Data were extracted from eligible studies, and risk of bias was assessed using the RoB2 tool. Certainty of evidence was evaluated by the GRADE tool. Meta analysis was conducted by using random effects model. Subgroup and sensitivity analyses were performed for primary outcomes. The publication bias was evaluated for outcomes reported by more than 10 trials using funnel plots and Egger's test.</div></div><div><h3>Results</h3><div>A total of 76 eligible RCTs involving 9862 patients with cancer and 45 type of adverse events were included. Meta-analysis revealed that compared with anticancer therapy alone, combination treatment with TCMI had lower risk for adverse events in bone marrow suppression (RR 0.60; 95 % CI,0.51, 0.70) and gastrointestinal issues (RR 0.69; 95 % CI, 0.63, 0.76). Also, TCMI was associated with decreased risk of leukopenia (RR 0.67; 95 % CI, 0.61, 0.74), thrombocytopenia (RR 0.66; 95 % CI, 0.57, 0.77), hemoglobin reduction (RR 0.76; 95 % CI, 0.68, 0.86), neutropenia (RR 0.74; 95 % CI,0.59, 0.93), anemia (RR 0.57; 95 % CI, 0.48, 0.68), liver adverse events (RR 0.63; 95 % CI, 0.54, 0.74), renal adverse events (RR 0.55; 95 % CI, 0.43, 0.70), neurotoxicity (RR 0.70; 95 % CI, 0.61, 0.81), cardiovascular toxicity (RR 0.42; 95 % CI, 0.23, 0.77), radiation-induced injuries (RR 0.43; 95 % CI, 0.34, 0.54]), oral mucositis (RR 0.46; 95 % CI, 0.36, 0.58), fatigue (RR 0.66; 95 % CI, 0.48, 0.90), fever (RR 0.52; 95 % CI, 0.32, 0.85), infection (RR 0.48; 95 % CI, 0.32, 0.72) and chemotherapy toxicity (RR 0.95; 95 % CI, 0.36, 0.86). GRADE assessment indicated high certainty for evidence in bone marrow suppression, hemoglobin reduction, liver adverse events, renal adverse events and oral mucositis.</div></div><div><h3>Conclusion</h3><div>TCMI can reduce adverse events of anticancer treatment and serve as an adjuvant therapy. It is necessary to strengthen the mechanism research of TCMI and carry out more large-scale, multi-center and well-designed clinical studies to evaluate the synergistic effects of TCMI in the future.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119969"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential impact of extracts from distinct Sceletium tortuosum chemotypes on central neurotransmitter concentrations in C57BL/6 mice","authors":"Catherine H. Kaschula ,&nbsp;Anna-Mart Engelbrecht ,&nbsp;Nokwanda P. Makunga ,&nbsp;Magriet Muller ,&nbsp;André de Villiers ,&nbsp;Kamano Mochoele Dube ,&nbsp;Sarel Brand ,&nbsp;Jo-Anne Stroebel ,&nbsp;Willem AL. van Otterlo ,&nbsp;De Wet Wolmarans","doi":"10.1016/j.jep.2025.119974","DOIUrl":"10.1016/j.jep.2025.119974","url":null,"abstract":"<div><h3>Ethnopharmacology relevance</h3><div><em>Sceletium tortuosum</em>, also known as kanna, or kougoed, has long been an integral part of the traditional medicinal practices of the San and Khoikhoi peoples of Southern Africa. Among the various <em>Sceletium</em> species, <em>S. tortuosum</em> is used for its mood-enhancing properties, attributed to the structurally related mesembrine-type alkaloids found therein. While significant research has focused on mesembrine and mesembrenone, the therapeutic potential of extracts from plants that produce more of the so-called “minor alkaloids”, remains unexplored.</div></div><div><h3>Aim of the study</h3><div>To assess the CNS modulatory effects of two chemotypes of <em>S. tortuosum,</em> wild-collected from two different geographic locations in South Africa (Touwsrivier and De Rust), each featuring different alkaloid profiles and with elevated minor alkaloid concentrations.</div></div><div><h3>Materials and methods</h3><div>Extracts from these chemotypes, as well as a vehicle control and a commercial extract, were administered to four groups of mice for 35 days. Mice were then euthanised, and their frontal cortices, striata and hippocampi dissected. Serotonin, dopamine, noradrenaline, glutamate, and gamma-aminobutyric acid (GABA) concentrations were analysed using LC-MS.</div></div><div><h3>Results</h3><div>Both chemotypes, compared to both control and commercial extract exposure, robustly increased noradrenaline and decreased GABA concentrations in all regions of the mouse brain analysed.</div></div><div><h3>Conclusion</h3><div>This finding may support a mood-enhancing effect of <em>S. tortuosum</em> and indicates its potential to modulate anxiety and stress processing, attention, and alertness. Alkaloid profiling further suggests that the mesembrine alcohols and sceletium A4 may be important contributors in driving these neurochemical changes.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"350 ","pages":"Article 119974"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acute toxicity study of ethanol extract of Potentilla freyniana Bornm rhizome in rats","authors":"Tingting Tian ,&nbsp;Yang Chen ,&nbsp;Qin He ,&nbsp;Yangmiao Jiao ,&nbsp;Lingqun Chen ,&nbsp;Maomao Shu ,&nbsp;Mujeeb ur Rehman ,&nbsp;Zaiqi Zhang ,&nbsp;Liang Cao","doi":"10.1016/j.jep.2025.119977","DOIUrl":"10.1016/j.jep.2025.119977","url":null,"abstract":"<div><h3>Ethnopharmacology relevance</h3><div>Rhizome of <em>Potentilla freyniana</em> Bornm, commonly known as “Ma Deng Ai” (MDA), is a widely used Dong ethnic medicine for the treatment of dysentery, malaria, bacterial infections, and traumatic bleeding.</div></div><div><h3>Aims of the study</h3><div>This study aims to evaluate the acute toxicity of MDA <em>in vivo</em>.</div></div><div><h3>Materials and methods</h3><div>In the acute toxicity study, MDA was administered to rats as a single oral dose of 1000 mg/kg, 2000 mg/kg, 4000 mg/kg, 8000 mg/kg, and 16000 mg/kg, along with a normal control group with distilled water in the same volume. Clinical signs of toxicity and general characteristics of animals were observed for 7 days. Water and food intake were recorded. After this hematological, biochemical, and histological analyses were performed.</div></div><div><h3>Results</h3><div>The results of the experiments performed illustrated significant changes in the rat's weight, water intake, and food consumption in all MDA treated groups. At a dose of 4000 mg/kg, rats began to show mild symptoms of difficulty in breathing. At a dose of 8000 mg/kg, rats showed more severe symptoms of breathing difficulty, leading to mortalities in this group. At a dose of 16000 mg/kg, rats showed most severe symptoms of breathing difficulty, with restlessness, and died within 6 h of oral administration, demonstrating the Lethal Dose-50 (LD₅₀) of 8510 mg/kg. The study indicates that the MDA treated groups showed significant changes in parts of hematological parameters such as mean corpuscle hemoglobin concentration (MCHC). The experiment groups have significant differences with control group in parameters of SGPT, SGOT, UREA, CRE and TG (<em>p</em> &lt; 0.05∗). The histopathological analysis showed that at a dose of 16000 mg/kg, the rat's lung, liver, and kidneys showed pathological changes such as vascular congestion and dilation.</div></div><div><h3>Conclusions</h3><div>The present study demonstrated the significant changes caused by MDA such as histopathological changes in the lung, liver, and kidneys. Oral doses of MDA to 8000 and 16000 mg/kg in rats showed oral-related toxicity or death of animals. The death was related to lung injury. Histopathological examination displayed observable cellular damage in cell morphology, nuclear characteristics, and organ tissue integrity in lungs, liver and kidneys. While the groups with the dosage of 1000, 2000 and 4000 mg/kg showed significant change in the food and water intake pattern. These findings provide a strong rationale for advancing to <em>in vivo</em> (safety and efficacy) studies to explore the biological and pharmacological profile of MDA in greater detail.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119977"},"PeriodicalIF":4.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144070439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trichilia silvatica extracts modulate the oxinflammatory response: an in vitro analysis","authors":"Leonardo Lopes Silveira ,&nbsp;Manoela Maciel dos Santos Dias ,&nbsp;Silvânia Mól Pelinsari ,&nbsp;Rosinéa Aparecida de Paula ,&nbsp;Adriano Simões Barbosa Castro ,&nbsp;Vera Lúcia de Almeida ,&nbsp;Reggiani Vilela Gonçalves","doi":"10.1016/j.jep.2025.119973","DOIUrl":"10.1016/j.jep.2025.119973","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Ethnopharmacological relevance&lt;/h3&gt;&lt;div&gt;Plants belonging to the Meliaceae family, such as &lt;em&gt;Trichilia silvatica&lt;/em&gt; C. DC., known as catiguá-branco, have attracted considerable interest in phytochemical research due to their diverse and significant secondary metabolites. &lt;em&gt;Trichilia silvatica&lt;/em&gt; has traditionally been employed in Brazilian medicine to treat inflammatory disorders. Moreover, studies have reported its antioxidant and antimicrobial properties, highlighting its potential therapeutic applications.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Aim of the study&lt;/h3&gt;&lt;div&gt;This study aimed to evaluate the potential of &lt;em&gt;Trichilia silvatica&lt;/em&gt; leaf and stem extracts in modulating OxInflammation in RAW264.7 macrophage cells following exposure to lipopolysaccharide (LPS) or hydrogen peroxide (H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;) and to elucidate the underlying mechanisms of action.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Material and methods&lt;/h3&gt;&lt;div&gt;The phytochemical composition of the extracts was characterized using thin-layer chromatography (TLC), HPLC equipped with a reversed-phase Hypersil C-18 column, and spectrophotometric method. Their antioxidant activity was evaluated using the 2,2-difenil-1-picrilhidrazil (DPPH) and Ferric Reducing Antioxidant Power (FRAP) assays. Cell viability was assessed via the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, alongside the determination of catalase (CAT) and superoxide dismutase (SOD) enzyme activities, as well as nitric oxide (NO) production in cells treated with the extracts and subsequently stimulated with H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;. Gene expression levels of Factor nuclear kappa B (NF-κB), Ciclooxygenase 2 (COX-2), Tumor necrosis factor alpha (TNF-α), Interleukin 10 (IL-10), and Hypoxia-inducible factor-1 (HIF-1) were quantified using RT-qPCR.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;&lt;em&gt;Trichilia silvatica&lt;/em&gt; extracts revealed the presence of terpenes/steroids, coumarins, condensed tannins, and phenolic acids, including chlorogenic and caffeic acids. The findings indicate that the leaf extract at 100 μg/ml and the stem extract at 100 μg/ml and 250 μg/ml preserved or enhanced cell viability, conferring protection against H&lt;sub&gt;2&lt;/sub&gt;O&lt;sub&gt;2&lt;/sub&gt;-induced oxidative stress. These concentrations significantly increased CAT activity, whereas SOD activity remained unaffected. Nitric oxide production was significantly reduced when cells were treated with 100 μg/ml and 250 μg/ml of both leaf and stem extracts. Moreover, FRAP value revealed an increase in antioxidant capacity at 250 μg/mL. Both leaf and stem extracts, at 100 μg/mL and 250 μg/mL, exhibited a DPPH radical scavenging capacity exceeding 75 % and downregulated the expression of pro-inflammatory cytokines, including NF-κB, TNF-α, and COX-2. Notably, the leaf extract at 250 μg/ml and the stem extract at 100 μg/mL upregulated the expression of IL-10 and H1F1.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusions&lt;/h3&gt;&lt;div&gt;These findings indicate that &lt;em&gt;Trichilia silvat","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119973"},"PeriodicalIF":4.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrating bioinformatic analysis, network pharmacology, molecular docking and experimental validation to explore the mechanism of Tian Long Cha against influenza virus","authors":"Wanqi Wang ,&nbsp;Zexing Chen ,&nbsp;Jinyi Zhu ,&nbsp;Yanling Xiang ,&nbsp;Yutao Wang ,&nbsp;Xinhua Wang ,&nbsp;Wanyi Huang","doi":"10.1016/j.jep.2025.119964","DOIUrl":"10.1016/j.jep.2025.119964","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Tong Long Cha (TLC) has been employed in clinical treatment for respiratory diseases, such as influenza, for over three decades. However, the precise mechanisms underlying its defense against influenza remain poorly understood.</div></div><div><h3>Aim of study</h3><div>This study aimed to investigate the bioactive compounds, pharmacological effects, and underlying mechanisms of TLC in combating influenza viruses.</div></div><div><h3>Methods</h3><div>Ultrahigh-performance liquid chromatography (UPLC)-Q-Exactive analysis was employed to identify the bioactive compounds of TLC. Key therapeutic targets and pathways involved in TLC's treatment for influenza were predicted using bioinformatics analysis, network pharmacology, and molecular docking. The antiviral effects of TLC and its principal active compound against various strains of influenza A viruses (A/Aichi/2/1968 (H3N2), A/PR/8/34 (PR8), A/California/04/2009 (CA04), A/HK/Y280/97(H9N2)) and influenza B virus (B/Lee/1940 (Lee)) were assessed using the cytopathic effect (CPE) inhibition assay and plaque reduction assay in MDCK cells. The therapeutic effects of TLC were evaluated using an influenza H3N2 virus-infected Balb/c mouse model. Quantitative PCR and Western blot analyses were employed to quantify the expression levels of key targets involved in TLC's potential mechanisms within A549 cells and the lungs of mice, as well as to investigate BCL's (baicalin) mechanism in A549 cells post-H3N2 infection. A co-culture model using Jurkat T cells and H3N2-infected A549 cells was also established to verify the modulation of key targets by TLC and BCL by using Western blot analyses.</div></div><div><h3>Results</h3><div>A total of 25 bioactive compounds were identified in TLC, with BCL being the predominant compound. TLC and BCL significantly inhibited the replication of the aforementioned influenza virus strains in MDCK cells. Additionally, TLC reduced weight loss, lung index, viral titers, lung tissue lesions, and levels of pro-inflammatory cytokines (IL-1β, IL-6, IL-8, IP-10, TNF-α), interferon (IFN-γ), and chemokine (MCP-1) in H3N2-infected mice. Mechanistic studies revealed that TLC and BCL upregulated Interleukin (IL)-2-inducible T cell kinase (ITK) and Tyrosine-protein kinase (FYN) expression while downregulating mitogen-activated protein kinase (MAPK14) expression, thereby modulating the T cell receptor signaling pathway, as predicted by bioinformatics analysis, network pharmacology, and molecular docking.</div></div><div><h3>Conclusion</h3><div>TLC could inhibit influenza virus replication and mitigate excessive inflammatory responses by modulating the T cell receptor signaling pathway, suggesting that it may serve as a promising therapeutic agent in traditional Chinese medicine for the treatment of influenza.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119964"},"PeriodicalIF":4.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical compounds from Allii Tuberosi Semen exhibiting anti-fibrotic effects on hepatic stellate cells","authors":"Rihong Zhao ,&nbsp;Xiping Liu ,&nbsp;Jinjin Liu ,&nbsp;Hao Wang ,&nbsp;Yuzi Cui ,&nbsp;Rui Yu ,&nbsp;Tiantian Jin ,&nbsp;Shuyun Wang ,&nbsp;Xinyu Qin ,&nbsp;Meijun Wang ,&nbsp;Li Ji ,&nbsp;Tianxiao Wang ,&nbsp;Aldarmaa Jalsrai ,&nbsp;Yue Cong","doi":"10.1016/j.jep.2025.119949","DOIUrl":"10.1016/j.jep.2025.119949","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Liver fibrosis involves changes in tissue structure and extracellular matrix composition due to multiple chronic liver diseases. Hepatic stellate cells (HSCs) play a crucial role in the development of liver fibrosis. Allii Tuberosi Semen, derived from the mature seed of <em>Allium tuberosum</em> Rottl.ex Spreng. (Liliaceae), is a traditional Chinese medicine known for its numerous bioactive properties, including hepatoprotective effects, sexual function improvement, immunity enhancement, anti-oxidation, and anti-microbial activities.</div></div><div><h3>Aim of the study</h3><div>This study aimed to identify effective ingredients in Allii Tuberosi Semen with anti-liver fibrosis properties.</div></div><div><h3>Materials and methods</h3><div>Extensive spectroscopic analysis was performed to identify the structures of the unreported compounds. Furthermore, these compounds were evaluated for anti-fibrotic effects on HSCs and the underlying molecular mechanisms.</div></div><div><h3>Results</h3><div>Five new steroidal saponins, compounds <strong>1</strong>–<strong>5</strong>, were isolated from Allii Tuberosi Semen, together with eight known compounds. Additionally, the carbon and hydrogen signals of compound <strong>6</strong> were assigned for the first time. These compounds significantly suppressed TGF-β1-induced HSCs activation via effectively reducing cell viability and migration while increasing the apoptosis rate. Further investigation was found that compound <strong>8</strong> inhibit the TGF-β1/Smad and PI3K/Akt pathways, increasing Bax/Bcl-2 ratio.</div></div><div><h3>Conclusions</h3><div>The results indicate that compounds <strong>1</strong>–<strong>12</strong> are effective inhibitors of liver fibrosis, making them promising agents for treating hepatic fibrosis.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119949"},"PeriodicalIF":4.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yin-chen Wu-ling powder inhibits MAPKs/CXCL1/CXCR2-induced neutrophil infiltration to alleviate LPS/D-GalN-induced acute liver failure","authors":"Liyue Lu ,&nbsp;Jiacheng Lin ,&nbsp;Feng Wei ,&nbsp;Weifan Huang ,&nbsp;Yali Sang ,&nbsp;Yuge Zhou ,&nbsp;Chang Yu ,&nbsp;Weian Yuan ,&nbsp;Yu Feng ,&nbsp;Xiaoni Kong","doi":"10.1016/j.jep.2025.119957","DOIUrl":"10.1016/j.jep.2025.119957","url":null,"abstract":"<div><h3>Ethnopharmacological relevance</h3><div>Acute liver failure (ALF) is the result of progression from acute liver injury with high mortality, and novel treatments are needed. Yin-chen Wu-ling powder (YWP), a traditional herbal medicine in China, has been used for treating acute liver injury for thousands of years. However, the mechanism of YWP is unknown.</div></div><div><h3>Aim of the study</h3><div>In vitro and in vivo studies were conducted to clarify YWP's protective effect on ALF and investigate its hepatoprotective mechanism.</div></div><div><h3>Materials and methods</h3><div>We established an LPS/D-GalN-induced ALF mouse model and in vitro system to evaluate the effect of YWP. We characterized YWP's chemical composition via UHPLC-Q-Exactive Orbitrap HRMS. Enzyme-linked immunosorbent assay, hematoxylin and eosin staining, immunohistochemistry and immunofluorescence, flow cytometry, qPCR, Western blot were used to discover key mechanisms both in vitro and in vivo.</div></div><div><h3>Results</h3><div>YWP alleviated liver dysfunction and liver necrosis. YWP reduced hepatocyte death and inflammatory responses. Importantly, YWP markedly inhibited neutrophil infiltration into the liver. We examined key chemokines that contribute to neutrophil recruitment. The results showed that YWP inhibited CXCL1, which is sourced from inflammation-activated hepatocytes. In addition, YWP inhibited TNF-α-induced CXCL1 transcription via the inhibition of MAPKs signaling in vitro. Furthermore, the anti-ALF effect of YWP was weakened when CXCL1/CXCR2 signaling was suppressed.</div></div><div><h3>Conclusion</h3><div>YWP alleviates inflammatory liver injury in ALF by suppressing neutrophil infiltration into the liver, potentially through inhibition of the MAPKs/CXCL1/CXCR2 axis. We suggest that YWP is a potential anti-inflammatory treatment for ALF.</div></div>","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":"349 ","pages":"Article 119957"},"PeriodicalIF":4.8,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Buzhong Yiqi Decoction Improves Inflammation and Oxidative Damage in Autoimmune Thyroiditis by Inhibiting Apoptosis via the SIRT1-Mediated Nrf2/NF-κB Axis.","authors":"Zhuo Zhao, Jiayun Li, Nan Song, Hao Gao, Dong Lin Liu, Zhe Jin, Yiran Chen, Xuanlin Guo, Ziyu Liu, Xiao Yang","doi":"10.1016/j.jep.2025.119967","DOIUrl":"https://doi.org/10.1016/j.jep.2025.119967","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Ethnopharmacological relevance: &lt;/strong&gt;Buzhong Yiqi decoction (BZYQ), a compound formula comprising eight traditional Chinese medicinal herbs, has been used clinically to treat various autoimmune diseases, including autoimmune thyroiditis (AIT). Our long-term clinical practice and research have shown that BZYQ demonstrates promising anti-inflammatory efficacy in the management of AIT, and the underlying pharmacological mechanisms involved warrant further exploration.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Aim: &lt;/strong&gt;To investigate the therapeutic effects and underlying mechanisms of the impact of BZYQ on AIT both in vitro and in vivo.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Materials and methods: &lt;/strong&gt;An AIT model was developed in NOD.H-2&lt;sup&gt;h4&lt;/sup&gt; mice by administering 0.05% NaI. The therapeutic efficacy of BZYQ on AIT was evaluated using hematoxylin-eosin (H&E) staining and enzyme-linked immunosorbent assay(ELISA). Oxidative stress and inflammation-related parameters, including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) were analyzed. Thyroid cell apoptosis was observed using TUNEL staining. Reverse transcription-quantitative polymerase chain reaction(RT-qPCR), Western blotting, immunohistochemistry, and immunofluorescence were performed. These techniques were used to determine the alterations in key genes and proteins involved in the sirtuin 1 (SIRT1)-mediated Nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/nuclear factor-kappaB p65 (NF-κB p65) axis regulating the apoptotic pathway in thyroid tissues of AIT mice after BZYQ intervention. Furthermore, lipopolysaccharide(LPS)was used to create a cellular model, which was then treated with BZYQ-containing serum (300 mg/kg). Confirmatory studies were conducted using a SIRT1 inhibitor. The protein levels of SIRT1, Nrf2, NF-κB p65, and caspase-3 in Nthy-ori 3-1 cells were analyzed to gain further mechanistic insights.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;BZYQ ameliorated thyroid pathology in AIT mice, reduced inflammatory cell infiltration, lowered inflammation scores, decreased serum levels of TGAb and TPOAb antibodies, and diminished the spleen index. These findings suggest that BZYQ has a protective effect against thyroid damage in AIT. BZYQ-M exhibited the most pronounced therapeutic efficacy. Mechanistically, BZYQ exerted its anti-inflammatory, antioxidant, and antiapoptotic effects by upregulating SIRT1, which subsequently promoted Nrf2 expression and inhibited NF-κB p65 activation. These changes led to increased protein expression and mRNA levels of its downstream targets, considerably suppressing the production of inflammatory cytokines (TNF-α and IL-6), attenuating oxidative stress (MDA, SOD, and CAT), regulating the expressions of apoptotic markers (Bax, Bcl2, CytC, and caspase-3), and reducing the apoptosis rate of thyroid cells. Further validation via in vitro experiments revealed that SIRT1 inhibitors can block the protective effects of","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"119967"},"PeriodicalIF":4.8,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to \"Bioactive compounds and mechanism of Xianglian pill in the treatment of gastric cancer: Network pharmacology analysis and experimental validation\" [J. Ethnopharmacol. 314 (2023) 116573].","authors":"Lei Yu, Luyao Sun, Qian Yu, Fang Xiong, Daibo Wang, Lin Pu, Fu Peng, Xiaofang Xie, Cheng Peng","doi":"10.1016/j.jep.2025.119928","DOIUrl":"https://doi.org/10.1016/j.jep.2025.119928","url":null,"abstract":"","PeriodicalId":15761,"journal":{"name":"Journal of ethnopharmacology","volume":" ","pages":"119928"},"PeriodicalIF":4.8,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}