Journal of endodontics最新文献

{"title":"Integrated Micro-Computed Tomography and Computational Fluid Dynamics Analysis of Stepwise Enlargement in Mesial Root Canals of Mandibular Molars.","authors":"Sofia Moura Furtado, Jorge N R Martins, Emmanuel João Nogueira Leal Silva, Mário Rito Pereira, Marco Aurélio Versiani, António Ginjeira","doi":"10.1016/j.joen.2025.09.001","DOIUrl":"10.1016/j.joen.2025.09.001","url":null,"abstract":"<p><strong>Introduction: </strong>To evaluate how stepwise enlargement in the mesial root canals of mandibular first molars affect shaping outcomes and irrigant dynamics.</p><p><strong>Methods: </strong>The shaping ability and irrigant flow patterns in mesial canals of mandibular first molars enlarged with ProTaper Next instruments (25/.06v, 30/.07v, 40/.06v) were evaluated using micro-computed tomography (micro-CT) and computational fluid dynamics (CFD). Instrument design and metallurgical properties were assessed by 3D scanning, scanning electron microscopy, energy-dispersive X-ray spectroscopy, and differential scanning calorimetry to ensure manufacturing consistency. An extracted mandibular molar with two curved mesial root canals was selected and scanned by micro-CT to generate 3D models for CFD simulation. Root canals were prepared stepwise with the ProTaper Next. Digital canal and needle geometries were reconstructed from micro-CT and scanning electron microscopy data. CFD simulations were conducted in ANSYS Fluent to calculate irrigant velocity, wall shear stress, and apical pressure. For shaping analysis, ten mesial canals from 5 mandibular molars were scanned before and after each preparation step. Canal volume, surface area, unprepared surfaces, and pericervical dentine thickness were quantified. Data were analyzed using repeated measures ANOVA with Bonferroni correction (P < .05).</p><p><strong>Results: </strong>Excluding for dimensions, all instruments shared similar metallurgical features and design characteristics, with minor surface imperfections. The open-ended needle consistently delivered irrigant to the working length, unlike the side-vented needle. Enlargement slightly increased apical velocity and irrigant density, particularly with the open-ended needle, which also generated higher wall shear stress and apical pressure. Canal volume and surface area increased significantly with larger files (P < .05), while unprepared areas decreased. Progressive canal enlargement significantly reduced minimal dentine thickness at the distal aspect of the mesial canals (most at 1-2 mm below the furcation), dropping from preoperative means of ∼1 mm to 0.60 mm after the 40/.06v instrument (P < .05), with the proportion of sections with dentine <0.5 mm increasing from 0% to 26%, while those >1 mm decreased markedly.</p><p><strong>Conclusions: </strong>Progressive apical enlargement improved shaping and irrigant dynamics, but additional increases offered limited benefits. Irrigant effectiveness was more influenced by needle design than by size alone, suggesting that optimizing delivery systems may enhance cleaning efficiency while minimizing unnecessary dentine removal.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Static versus Dynamic Cyclic Fatigue Resistance in NiTi Endodontic Instruments with Different Alloy Treatments at Body Temperature.","authors":"Tareq Abdulkareem, Mohamed Jamal, Amre Atmeh, Haitham Elbishari, Amar H Khamis, Hyeon-Cheol Kim, Rashid El Abed","doi":"10.1016/j.joen.2025.09.004","DOIUrl":"10.1016/j.joen.2025.09.004","url":null,"abstract":"<p><strong>Introduction: </strong>This study evaluated the fatigue resistance of 2 nickel-titanium engine-driven file systems with identical geometries and different heat treatments tested under static and dynamic conditions in simulated root canals.</p><p><strong>Methods: </strong>Cyclic fatigue tests were conducted using ProTaper Universal (PTU) and ProTaper Gold (PTG) instruments with a curvature of 35° and a radius of 6 mm in both static and dynamic modes at body temperature using a customized cyclic fatigue testing device. The number of cycles to fracture was recorded. A scanning electron microscope was used to examine the fracture surfaces of fractured fragments.</p><p><strong>Results: </strong>The cyclic fatigue resistances of PTU and PTG varied depending on the testing methods (static or dynamic). Statistically significant differences were observed between the 2 file systems, with PTG demonstrating superior fatigue resistance in both testing modes. Notably, dynamic testing resulted in a higher number of cycles to fracture for both PTU and PTG compared to static testing. However, the relative improvement in the cyclic fatigue resistance of PTG over PTU remained consistent under both conditions. The scanning electron microscope analysis of the cross-sectional and longitudinal aspects of the fractured specimens revealed microcrack initiation regions, overloaded quick fracture areas, and microcracks. PTG demonstrated a lower concentration of microcracks compared to PTU.</p><p><strong>Conclusions: </strong>This study confirmed that heat treatment significantly enhances the cyclic fatigue resistance of NiTi instruments and static mode reduces the fatigue resistance of the file. Heat-treated files showed better property due to their martensitic phase, which improves flexibility and reduces crack initiation.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145033497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Validation of Smartphone-Enabled Pulse Oximetry for Objective Pulp Vitality Assessment: A Diagnostic Accuracy Study.","authors":"Celso Luiz Caldeira, Stephanie Isabel Diaz Zamalloa, Claudia Regina Guimaro Sakitani, Fernando Branco Barletta, Marinella Holzhausen","doi":"10.1016/j.joen.2025.09.003","DOIUrl":"10.1016/j.joen.2025.09.003","url":null,"abstract":"<p><strong>Introduction: </strong>Pulse oximetry exhibits great potential for use in endodontic diagnosis as an effective method to assess pulp vitality. Cell phone-integrated oximeters represent an emerging alternative that may offer greater accessibility. This study aimed to investigate the relation between pulp oxygenation rates (%SpO₂) and clinical diagnosis of healthy pulp (HP), reversible pulpitis (RP), symptomatic irreversible pulpitis (IP), or pulp necrosis (PN), comparing 2 pulse oximeters (conventional [PO1] and mobile-connected).</p><p><strong>Methods: </strong>Thirty affected premolars were grouped according to the clinical diagnosis of RP, IP, or PN and compared to vitality diagnosis by %SpO₂ obtained by both pulse oximeters: PO1 and by smartphone-based (PO2). For each affected tooth, 2 additional teeth (adjacent and contralateral) with HP were tested. Statistical analysis was performed using one-way ANOVA with Tukey's post-hoc test.</p><p><strong>Results: </strong>The mean %SpO₂ values measured by PO1 were 87.7% for RP, 80.5% for IP, 73.2% for PN, and 91.89% for HP. Corresponding values obtained with PO2 were 85.6% (RP), 79.3% (IP), 72.1% (PN), and 90.1% (HP). Significant differences in %SpO₂ were observed among RP, IP, and PN groups (P < .05), but not between healthy control subgroups (adjacent and contralateral). PO1 consistently recorded higher oxygen saturation than PO2 across all clinical conditions (P < .05), demonstrating superior discriminatory capability.</p><p><strong>Conclusions: </strong>Both pulse oximeters demonstrated significant clinical value for pulp vitality assessment and differentiation of pulpal inflammation stages and necrosis. The smartphone-adapted oximeter has proven to be comparable to PO1 in diagnostic performance, while offering data collection efficiency and clinical workflow integration.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autogenous Transplantation of an Immature Undersized Third Molar to Rehabilitate a Cyst-associated Lateral Incisor Defect: A 3-Year Case Report.","authors":"Cheng Bi, Zirui Bian, Bin Peng, Hui Wang, Jin Jiang, Yifan Chen, Jingyi Tan","doi":"10.1016/j.joen.2025.08.021","DOIUrl":"10.1016/j.joen.2025.08.021","url":null,"abstract":"<p><p>Severe periapical cysts frequently result in tooth loss with concomitant severe alveolar bone destruction, precluding immediate implant placement. Autogenous tooth transplantation, which involves transplanting the patient's own tooth to the recipient site, offers a biological approach to reconstruct both the dentition and supporting bone structures. This report presents a 25-year-old female with a mobile, discolored maxillary right lateral incisor (#12) exhibiting grade 3 mobility due to a large periapical cyst (extending to #14 on cone-beam computed tomography) with root developmental arrest, secondary to Oehlers type II dens invaginatus. Autogenous transplantation was performed using an immature impacted third molar (#28) with compatible root morphology. Despite pulp necrosis requiring root canal treatment at 1 month, functional stability was achieved by 3 months. Three-year follow-up demonstrated complete bone healing, intact periodontal ligament space, and absence of resorption or ankylosis on cone-beam computed tomography. This case underscores the promising role of autogenous tooth transplantation as a viable treatment option for complex dental scenarios.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sexual Dimorphism in Apical Periodontitis Severity Detected by CBCT.","authors":"Lucas R A Estrela, Carlos Estrela, Mike R Bueno, Anibal Diogenes","doi":"10.1016/j.joen.2025.08.020","DOIUrl":"10.1016/j.joen.2025.08.020","url":null,"abstract":"<p><strong>Introduction: </strong>Although several elegant studies have reported apical periodontitis prevalence in different populations, far less is known about its associated bone loss and its correlation to sex and age. Thus, this study investigated the impact of sex and age differences on the severity of apical periodontitis (AP) using cone-beam computed tomography (CBCT) and a volumetric periapical index (CBCT-PAI).</p><p><strong>Methods: </strong>CBCT scans of 401 patients (1,027 teeth) were analyzed by calibrated examiners in CBCT-PAI. Chi-square tests were used to assess associations between AP, sex, and age with other variables such as the presence and quality of endodontic treatment and coronal restorations. Robust Poisson regression models were used to evaluate the relationships between dependent and independent variables, with significance set at P < .05.</p><p><strong>Results: </strong>The prevalence of AP was greater in female patients compared to males (66.9% vs 33.1%). Notably, men showed a significantly higher prevalence of larger lesions (CBCT-PAI 4 and 5) than women. Apical periodontitis was more common in root-filled teeth than primary infections, particularly posterior teeth. Lastly, AP was more commonly found in teeth with inadequate root canal fill or coronal restoration, while an intracanal post did not affect its prevalence.</p><p><strong>Conclusions: </strong>AP was more common in women. However, the osteolytic lesions in females were smaller than those seen in males, who demonstrated a greater prevalence of severe lesions. These findings emphasize the importance of early diagnosis and treatment and highlight the significant sexual dimorphism in the pathophysiology of AP.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145000705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Location of the Apical Constriction in Relation to the Flash Bar of an Apex Locator: A Micro-CT Study","authors":"Haya Mohamed El Sweify BDS ,&nbsp;Nelly Abdelsalam BDS, MSD, DDS ,&nbsp;Nasr Hashem BDS, MSD, DDS ,&nbsp;Ahmed Mahmoud Fouda BDS, MSD, DDS ,&nbsp;Christoph Bourauel Dr. rer. nat. ,&nbsp;Marwa Sharaan BDS, MSD, DDS","doi":"10.1016/j.joen.2025.06.009","DOIUrl":"10.1016/j.joen.2025.06.009","url":null,"abstract":"<div><h3>Introduction</h3><div><span>This study aimed to locate the anatomical apical constriction (AC) of root canals using micro-computed tomography (micro-CT) and microscopy, and to correlate these findings with the flash bar of the Root ZX </span>electronic apex locator. The objective was to identify the protocol that positions the file closest to the AC.</div></div><div><h3>Methods</h3><div>The working lengths of 30 extracted single-rooted teeth were measured using Root ZX electronic apex locator with 3 employment protocols. Group I: Files were advanced in the canals to the “APEX” mark and then withdrawn to the “end of the Blue zone” mark. Group II: Files were advanced to the “APEX” mark and then withdrawn to the “0.5” mark. Group III: Files were advanced only to reach the “APEX” mark. Files were then cemented in place and examined using micro-CT and surgical operating microscope to identify the actual location of the AC. The discrepancies between the locations of the file's tip and the ACs were assessed by 3D slicer software and statistically analyzed.</div></div><div><h3>Results</h3><div>Significant differences were observed among the 3 groups based on micro-CT and surgical operating microscope measurements. The end of the blue zone achieved 100% accuracy, the 0.5 mark 85%–80%, and the apex mark protocol 0%–5% (within a tolerance of ±0.5 mm). An overall positive correlation was found between micro-CT and surgical microscopy measurements (r = 0.91). Working length overestimation beyond the major foramen occurred in 30% of the samples in the 0.5 mark protocol and 60% of the Apex mark group.</div></div><div><h3>Conclusions</h3><div>The “end of the Blue zone” mark protocol provided the greatest accuracy without file extrusion, followed by the “0.5” mark. The “APEX” mark protocol is not recommended since this protocol placed the file at an average of 1.24 mm apical to the AC.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 9","pages":"Pages 1278-1284"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144340163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Head Angulation in Cone-beam Computed Tomography Acquisition: Effect on the Diagnostic Accuracy of Vertical Root Fracture Detection","authors":"Marcela Tarosso Réa DDS, MS, PhD ,&nbsp;Lara Carvalho DDS ,&nbsp;Maria Fernanda Silva Andrade-Bortoletto DDS, MS ,&nbsp;Hugo Gaêta-Araujo DDS, MS, PhD ,&nbsp;Christiano de Oliveira-Santos DDS, MS, PhD ,&nbsp;Sergio Lins de-Azevedo-Vaz DDS, MS, PhD ,&nbsp;Deborah Queiroz Freitas DDS, MS, PhD","doi":"10.1016/j.joen.2025.05.020","DOIUrl":"10.1016/j.joen.2025.05.020","url":null,"abstract":"<div><h3>Introduction</h3><div>Vertical root fractures (VRFs) detection in cone-beam computed tomography (CBCT) images can be challenging when gutta-percha artifacts are present. This study aimed to evaluate how anteroposterior and laterolateral head angulations (APHA and LLHA, respectively), as well as tooth position (anterior or posterior region), influence the diagnostic accuracy of VRFs detection in CBCT for endodontically treated teeth.</div></div><div><h3>Methods</h3><div><span><span>Twenty single-rooted teeth were endodontically treated. A phantom was created using a human mandible and ballistic gelatin. </span>CBCT scans of each tooth, placed in both the anterior and posterior regions, were acquired with the phantom positioned at the standard position of 0°, at APHA of 30° and 45°, and at a LLHA of 20°, totaling 320 scans. The presence or absence of VRFs was assessed by 6 examiners using a 5-point scale. Area under the receiver operating characteristic curve, sensitivity, and specificity were analyzed using two-way repeated measures analysis of variance and Sidak's </span><em>post hoc</em><span> test. Intra- and interexaminer agreement was evaluated using the weighted Kappa coefficient (α = 0.05).</span></div></div><div><h3>Results</h3><div>In general, the 30° and 45° APHA significantly increased area under the receiver operating characteristic curve and sensitivity values for VRF detection in both anterior and posterior regions compared to 0° and 20° LLHA. Angulations and tooth position did not influence specificity. For specificity values, the 45° APHA exhibited a significantly higher value than the other angulations in the anterior region.</div></div><div><h3>Conclusions</h3><div>VRFs diagnosis enhanced at 30° and 45° APHA, regardless the region.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 9","pages":"Pages 1235-1241"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144199301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental Trauma Evo – Development of an Artificial Intelligence-powered Chatbot to Support Professional Management of Dental Trauma","authors":"Diulia Pereira Bubna DDS ,&nbsp;Pedro Felipe de Jesus Freitas DDS ,&nbsp;Aline Xavier Ferraz DDS ,&nbsp;Allan Abuabara MSc ,&nbsp;Flares Baratto-Filho PhD ,&nbsp;Bianca Marques de Mattos de Araujo PhD ,&nbsp;Erika Calvano Kuchler PhD ,&nbsp;Liliane Roskamp PhD ,&nbsp;Angela Graciela Deliga Schroder PhD ,&nbsp;Cristiano Miranda de Araujo PhD","doi":"10.1016/j.joen.2025.05.012","DOIUrl":"10.1016/j.joen.2025.05.012","url":null,"abstract":"<div><h3>Introduction</h3><div>Dental trauma is a public health issue that requires proper clinical management to minimize complications. Artificial intelligence-based technologies can support decision-making by providing standardized guidance. This study developed and evaluated an artificial intelligence-powered chatbot to assist professionals in managing dental trauma.</div></div><div><h3>Methods</h3><div>The Dental Trauma Evo chatbot was developed based on the guidelines of the International Association of Dental Traumatology<span>, utilizing a rule-based system to ensure recommendations aligned with established clinical protocols. The chatbot was implemented in Python, integrated with the ChatGPT-4 API, and made available via Streamlit, enabling interactions in over 50 languages. Validation was conducted by specialists in endodontics<span> and pediatric dentistry, who assessed the clarity and consistency of the responses. Performance was evaluated through 384 interactions covering 32 types of trauma, assessing the accuracy and completeness of the recommendations.</span></span></div></div><div><h3>Results</h3><div>The chatbot achieved 100% accuracy in recommending appropriate clinical management. However, some initial responses did not include all treatment options outlined in the guidelines, particularly in cases of enamel, dentin, and pulp-exposed fractures in primary teeth<span>, where only 53% of responses were complete. After adjustments to the code, the second round of testing showed significant improvements, reaching 100% completeness in most cases. Only the recommendations for the reimplantation of avulsed teeth with an open apex reached 93%, indicating the need for further refinements.</span></div></div><div><h3>Conclusions</h3><div>The artificial intelligence-powered chatbot demonstrated high performance in standardizing the management of dental trauma, proving its potential to assist professionals in decision-making by providing quick and precise responses aligned with international guidelines.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 9","pages":"Pages 1229-1234"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alkaline Treatment Enhances the Anti-Inflammatory and Reparative Potential of Dentin Matrix Proteins in Inflamed Pulp","authors":"Peipei Jia PhD ,&nbsp;Jingyi Li DDS ,&nbsp;Jilin Wu DDS ,&nbsp;Xueying Li BS ,&nbsp;Sicong Mao DDS ,&nbsp;Sainan Wang PhD ,&nbsp;Yanmei Dong DDS, PhD","doi":"10.1016/j.joen.2025.06.002","DOIUrl":"10.1016/j.joen.2025.06.002","url":null,"abstract":"<div><h3>Introduction</h3><div>To investigate the effects of dentin matrix-extracted complex proteins (DMEPs), especially basic-extracted DMEP (bDMEP), on the regulation of pulp inflammation and repair processes.</div></div><div><h3>Methods</h3><div><span><span><span>Protein components of neutral-extracted DMEP (nDMEP) and bDMEP were analyzed using a cytokine array and ELISA. Human dental pulp cells stimulated by </span>lipopolysaccharide<span><span> (LPS-hDPCs) were treated with nDMEP and bDMEP. CCK-8 and transwell experiments were employed to assess cell proliferation and migration. The expression levels of genes and proteins related to inflammation and odontogenic differentiation were analyzed through quantitative real-time polymerase chain reaction, ELISA, </span>immunofluorescence staining, and </span></span>western blot. </span><em>In vivo</em><span><span>, gelatin sponges containing nDMEP or bDMEP were employed as pulp-capping materials in a rat </span>pulpitis model. Histological staining was performed to evaluate inflammation and tissue repair in inflamed pulp tissue.</span></div></div><div><h3>Results</h3><div><span>Compared with nDMEP, alkaline treatment significantly increased the content of multiple growth factors (C-X-C motif chemokine<span><span><span> ligand 14, bone morphogenetic protein-2, transforming growth factor-beta1, transforming growth factor-beta2, osteopontin, insulin-like growth factor binding protein-2) and decreased some </span>inflammatory mediators (macrophage inflammatory protein-1, interleukin (IL)-17, intercellular adhesion molecule-2, neural cell adhesion molecule-1) in DMEP. bDMEP enhanced the proliferation and chemotaxis of LPS-hDPCs. DMEP, especially bDMEP, inhibited the levels of proinflammatory cytokines (IL-1β, IL-6, tumor necrosis factor-α, CXCL10) while enhancing the expression of odontogenesis-related genes (dentin matrix protein 1, dentin sialophosphoprotein, </span>collagen type I, alkaline phosphatase) in LPS-hDPCs. </span></span><em>In vivo</em><span>, bDMEP effectively reduced inflammatory infiltration and facilitated formation of dentin-like mineralization in a rat pulpitis model.</span></div></div><div><h3>Conclusions</h3><div>Alkaline treatment significantly improved the bioactivity of growth factors derived from the dentin matrix, alleviating the pulpal inflammatory response and facilitating biomimetic repair in inflamed pulp tissue.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 9","pages":"Pages 1215-1228"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144248172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Donor Honor Roll","authors":"","doi":"10.1016/S0099-2399(25)00566-7","DOIUrl":"10.1016/S0099-2399(25)00566-7","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 9","pages":"Pages 1316-1337"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145048166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}