Journal of endodontics最新文献

{"title":"Diagnostic Performance of Iterative Reconstruction of Cone-beam Computed Tomography for Detecting Vertical Root Fractures in the Presence of Metal Artifacts.","authors":"Matheus Barros-Costa, Gustavo Santaella, Christiano Oliveira-Santos, Deborah Queiroz Freitas, William C Scarfe, Francisco Carlos Groppo","doi":"10.1016/j.joen.2025.02.008","DOIUrl":"10.1016/j.joen.2025.02.008","url":null,"abstract":"<p><strong>Introduction: </strong>Vertical root fractures (VRFs) are challenging to detect, especially in endodontically treated teeth. Cone-beam computed tomography (CBCT) has improved VRF detection over periapical radiography but is limited by metal artifacts. Iterative Reconstruction (IR) algorithms are an alternative to the traditional Filtered-Back Projection (FBP) and have been shown to reduce image noise due to metal artifacts. This study aimed to evaluate the impact of IR on VRF detection in the presence of metal artifacts from intracanal materials and/or adjacent implants.</p><p><strong>Methods: </strong>This ex-vivo study included 22 single-rooted teeth (10 fractured, 12 nonfractured) placed in a human mandible phantom. CBCT scans were performed using Midmark EIOS CBCT with both reconstruction methods (FBP and IR), with and without metal artifact reduction (MAR) tool activation. Clinical conditions included the presence or absence of gutta-percha and adjacent titanium dental implants. Five oral radiologists evaluated the anonymized images for VRF presence using a 5-point scale. Diagnostic accuracy, sensitivity, specificity, and intra- and interobserver agreement were assessed using receiver operating characteristic curves and multiway ANOVA.</p><p><strong>Results: </strong>The area under the curve values ranged from 0.60 to 0.85, with no significant differences between reconstruction methods (P > .05). Sensitivity and specificity were generally unaffected by reconstruction method or MAR activation (P > .05), except when MAR reduced sensitivity in FBP with gutta-percha (P < .05). Metal artifacts from gutta-percha and implants negatively impacted VRF detection.</p><p><strong>Conclusions: </strong>The reconstruction method did not significantly influence VRF detection, suggesting that either method can be used in clinical practice without compromising diagnostic accuracy, even in the presence of metal artifacts.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Endodontically Treated Teeth in Patients Undergoing Treatment for Painful Temporomandibular Disorders: An Observational Study.","authors":"Nara Sarmento Macedo Signorelli, Camilla Christian Gomes Moura, Roger Ferreira Borges, Paulo Cézar Simamoto Júnior, Jennifer L Gibbs","doi":"10.1016/j.joen.2025.02.006","DOIUrl":"10.1016/j.joen.2025.02.006","url":null,"abstract":"<p><strong>Introduction: </strong>Temporomandibular disorders (TMDs) and endodontic pain often present overlapping symptoms, complicating the diagnosis and treatment of patients experiencing orofacial pain.</p><p><strong>Methods: </strong>This observational retrospective cohort study aims to determine the prevalence of endodontically treated teeth on the side of pain complaint in patients with TMD, and to explore the association between TMD diagnoses and the presence of endodontically treated teeth. Dental records of patients treated at the TMD and Pain clinic at the Federal University of Uberlândia was reviewed. The study included 85 patients, and demographic data, TMD diagnosis, and the side of referred pain were extracted from the records. Diagnostic imaging exams, including panoramic and periapical radiographs, and cone-beam computed tomography, were analyzed to count the number of endodontically treated teeth on each side of the mouth.</p><p><strong>Results: </strong>The study population consisted of 88% (n = 70) women, with a mean age of 40.5 ± 16.3 years. A significant association was found between the side of TMD pain and the number of endodontically treated teeth, with more treated teeth on the side of pain complaint (median = 1) compared to the pain-free side (median = 0), with P < .001. When analyzing specific TMD diagnoses, the association was significant in muscle-related and mixed TMD diagnoses but not in joint-related TMD pain. Additionally, a binary analysis confirmed this association, showing that patients were significantly more likely to have at least 1 endodontically treated tooth on the side of TMD pain compared to the nonpain side (χ² = 13.06, P = .0003).</p><p><strong>Conclusion: </strong>This study underscores the importance of comprehensive diagnostic evaluations in patients with orofacial pain to avoid unnecessary endodontic treatments and ensure appropriate care. Further research is needed to explore the underlying mechanisms linking endodontic and TMD pain and to validate these findings in diverse populations.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Outcomes of Regenerative Endodontic Procedure: Periapical Healing, Root Development, and Intracanal Calcification.","authors":"Minju Song, Hoi In Jung, Sahng Gyoon Kim","doi":"10.1016/j.joen.2025.02.007","DOIUrl":"10.1016/j.joen.2025.02.007","url":null,"abstract":"<p><strong>Introduction: </strong>This retrospective study aimed to investigate the clinical outcomes and prognostic factors of regenerative endodontic procedure in terms of the resolution of apical periodontitis (AP), root development (RtDev), and intracanal calcification (IC).</p><p><strong>Methods: </strong>The clinical outcomes of a total of 111 cases from 94 patients who underwent regenerative endodontic procedures between 2015 and 2020 were assessed based on clinical and radiographic evaluations.</p><p><strong>Results: </strong>The cumulative survival rate was 85.15%, and RtDev was identified in 67.33% of cases. The incidence of IC has increased over time, reaching 43.45%. The median follow-up time was 1267 days (95% confidence interval, 991-∞) for AP healing and RtDev and 776 days (95% confidence interval, 563-1754) for IC. The 4th stage of RtDev and intracanal medication for less than 14 days or more than 60 days were associated with significantly lower AP healing (P < .05). Patients younger than 8 years of age, absence of a periapical lesion, 2nd and 3rd stage of RtDev, and 14-30 days of intracanal medication were associated with a higher incidence of RtDev (P < .05). Dens evaginatus was associated with a significantly higher incidence of IC (P < .05).</p><p><strong>Conclusions: </strong>The stage of RtDev and duration of intracanal medication significantly affected AP healing and RtDev. Additionally, age and the presence of a periapical lesion were found to influence RtDev, whereas etiology was identified as the only predictor of IC.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation Between Fibrous Area Ratio and Numbers of Cycles to Fracture Resistances of Nickel-Titanium Files.","authors":"Hyeonu Jo, Sang Won Kwak, Asgeir Sigurdsson, Hyeon-Cheol Kim","doi":"10.1016/j.joen.2025.02.004","DOIUrl":"10.1016/j.joen.2025.02.004","url":null,"abstract":"<p><strong>Introduction: </strong>This study aimed to evaluate the correlation between the fibrous area ratio and the number of cycles to failure (NCF) in terms of fatigue fracture resistance of nickel-titaniumrotary files.</p><p><strong>Methods: </strong>ProTaper Universal (PTU) and ProTaper Gold (PTG) files of size F2 (25 mm) were tested to make cyclic fatigue fracture using a custom-made device simulating a 35° canal curvature (n = 24). Using the fractured fragments, scanning electron microscopy was taken to analyze the fracture surfaces, focusing on fibrous areas on the cross-sections. Fibrous area ratios were calculated using ImageJ software, and NCF was determined by multiplying the time to fracture by rotational speed. Statistical analysis was performed to evaluate differences between groups and assess the correlation between fibrous area ratio and NCF (P < .05).</p><p><strong>Results: </strong>Scanning electron microscopy analysis revealed a higher fibrous area ratio in PTU files (P < .05). The correlation analysis indicated a weak relationship between fibrous area ratio and NCF in both groups. PTG files demonstrated significantly higher NCF compared to PTU files (P < .05). PTG group had a significantly lower fibrous area ratio than the PTU group (P < .05).</p><p><strong>Conclusions: </strong>While PTG files show improved cyclic fatigue resistance due to thermal processing, the fibrous area ratio is not a strong representing factor or predictor of NCF. Heat treatment influences the property of fracture resistance more significantly than propagation process of cyclic fatigue fracture.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nine-year Follow-up of Autotransplantation in the Maxillary Anterior Region: Replacing a Fused Incisor with a Supernumerary Tooth.","authors":"Berkant Sezer, Betül Şen Yavuz, Bora Korkut, Ali Menteş","doi":"10.1016/j.joen.2025.02.003","DOIUrl":"10.1016/j.joen.2025.02.003","url":null,"abstract":"<p><p>The goal of tooth autotransplantation is to restore a missing tooth by using a functional tooth from the same patient. Although this procedure is gaining more recognition as a viable treatment option in dentistry, it is important to monitor and report long-term results. This case report presents the long-term follow-up of a fused maxillary central incisor that was extracted and replaced with a supernumerary tooth. Although the donor tooth displayed favorable characteristics, including an intact periodontal ligament and minimal extraoral time, which contributed to the success of the transplantation, it also presented certain disadvantages, such as having a fully developed root apex. Over a 9-year follow-up period, no clinical symptoms indicative of functional failure were observed. However, external cervical root resorption, first detected at the 6-year follow-up, progressed by the ninth year. Despite the lack of clinical symptoms, radiographic findings suggested that the root resorption was a significant concern. This case underscores the potential for long-term success of autotransplanted teeth, even in complex scenarios involving fused teeth. However, it also highlights the importance of thorough, long-term monitoring to detect complications such as root resorption. The findings suggest that, while autotransplantation can offer favorable outcomes, careful management and periodic evaluations are essential to ensure the longevity of the transplanted tooth.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143433265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review of ChatGPT as a Reliable Source of Scientific Information Regarding Endodontic Local Anesthesia.","authors":"Al Reader, Melissa Drum","doi":"10.1016/j.joen.2025.02.002","DOIUrl":"10.1016/j.joen.2025.02.002","url":null,"abstract":"<p><strong>Introduction: </strong>ChatGPT is an artificial intelligence chatbot, developed by OpenAI, which uses Deep Learning technology for information processing. The chatbot uses natural language processing and machine learning algorithms to respond to users' questions. The purpose of this study was to review ChatGPT responses to determine if they were a reliable source of scientific information regarding local anesthesia for endodontics.</p><p><strong>Methods: </strong>Sixteen representative questions pertaining to local anesthesia for endodontics were selected. ChatGPT was asked to answer the 16 questions and provide supporting references. Each provided ChatGPT reference was evaluated for accuracy using NLM NIH.GOV (PubMed), Google Scholar, journal citations, and author citations. Peer-reviewed, evidence-based literature citations related to the initial questions were collected by the authors. The 2 authors independently compared the answers of the ChatGPT to the peer-reviewed, evidence-based literature using a 5-answer Likert-type scale.</p><p><strong>Results: </strong>ChatGPT was reliable 50% of the time when compared to the peer-reviewed, evidence-based literature. That is, ChatGPT had the same literature-based response as our peer-reviewed, evidence-based literature in 16 of the 32 questions. Of the 51 total references for Chatbot, 59% (30 of 51) had the wrong reference, 12% (6 of 51) of the references couldn't be retrieved and 18% (9 of 51) of the references were hallucinations (made up references).</p><p><strong>Conclusions: </strong>Artificial intelligence needs further training to be trusted for accurate information in the field of endodontic anesthesia. ChatGPT should continue to improve to provide reliable information for providers and patients alike.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Wine Polyphenols on the Inflammatory Profile of Induced Apical Periodontitis in Rats.","authors":"Rafaela Ricci, Bharbara de Moura Pereira, Julissa Denisse Arguello Alvarado, Romulo de Oliveira Sales-Junior, Nathália Evelyn da Silva Machado, Doany Cevada Dos Santos, Felipe Haddad Martim Pederro, Marciane Magnani, Marcos Dos Santos Lima, Edilson Ervolino, Luciano Tavares Ângelo Cintra, Anil Kishen, João Eduardo Gomes-Filho","doi":"10.1016/j.joen.2025.01.023","DOIUrl":"10.1016/j.joen.2025.01.023","url":null,"abstract":"<p><strong>Introduction: </strong>This study evaluated the impact of dealcoholized red wine polyphenols on the inflammation and lesion volume associated with apical periodontitis (AP) in rats.</p><p><strong>Methods: </strong>Thirty-two Wistar rats receiving AP induction were arranged as follows: Control Group, Dealcoholized Red Wine Group (DRW), Red Wine Group, and Alcohol Group (ALC). Solutions were administered daily in a volume of 4.28 mL/kg via gavage for 45 days. Mandibles and maxillae were removed for histologic, immunohistochemical (IL-1β, IL-10, tumor necrosis factor-alpha, receptor activator of nuclear factor κB ligand, osteoprotegerin [OPG], and tartrate-resistant acid phosphatase), and micro-computed tomography analyses of the AP site. A statistical analysis was performed with a significance level of 5%.</p><p><strong>Results: </strong>Inflammation and TRAP-positive cell count were similar for DRW and Red Wine Group, but lower when compared to Control Group and ALC (P < .001). The immunohistochemical expression of OPG was higher for DRW than for ALC (P < .05). A larger lesion volume was observed in ALC compared to other groups (P < .001).</p><p><strong>Conclusions: </strong>Prophylactic administration of dealcoholized red wine significantly reduced inflammation, decreased the number of TRAP-positive cells, enhanced OPG expression, and reduced lesion volume compared to water and alcohol solutions.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143390990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Five-year Outcomes of Root Canal Treatment Comparing Sealer-based Obturation with Calcium Silicate-based Sealer to Continuous Wave of Condensation: A Randomized Controlled Trial.","authors":"Minkyeong Seog, Seung-Kyung Kwon, Do-Hyun Kim, Su-Jung Shin, Il-Young Jung","doi":"10.1016/j.joen.2025.02.001","DOIUrl":"https://doi.org/10.1016/j.joen.2025.02.001","url":null,"abstract":"<p><strong>Introduction: </strong>While sealer-based obturation (SBO) with calcium silicate-based sealers has shown promise in short-term studies, including a previous randomized controlled trial comparing it to the continuous wave of condensation (CWC) technique, long-term evidence on its clinical performance remains limited. This study addresses this critical gap by following the same patient cohort for five years to evaluate the long-term clinical and radiographic outcomes of both techniques and investigate factors influencing long-term success.</p><p><strong>Methods: </strong>Patients were initially randomized at the time of obturation into two treatment groups, based on the obturation protocol: CWC with AH Plus sealer and SBO with Endoseal TCS. Post-obturation pain was assessed using a numerical rating scale. Root canal obturation quality was evaluated for sealer extrusion, filling voids, and root filling level. Five-year follow-up examinations assessed healing based on periapical index score reduction and symptom resolution. Statistical analysis included chi-square or Fisher's exact tests and multivariate logistic regression.</p><p><strong>Results: </strong>Of the initial 93 teeth, 60 (64.5% recall rate) were available for analysis after five years (mean follow-up: 62 months). Four teeth were extracted for non-endodontic reasons, leaving 56 teeth (CWC: 33; SBO: 23) for evaluation. Success rates showed no significant differences between groups under both loose (overall: 83.9%; CWC: 81.8%; SBO: 87.0%) and strict criteria (overall: 76.8%; CWC: 75.8%; SBO: 78.3%). No analyzed factors significantly correlated with long-term success.</p><p><strong>Conclusion: </strong>The SBO technique with Endoseal TCS demonstrated comparable five-year outcomes to the CWC technique, supporting its potential as a less technique-sensitive and clinically viable alternative for root canal obturation.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Postoperative Pain Following the Use of the TruNatomy and XP-endo Shaper Systems: A Randomized Clinical Study.","authors":"Christianne Velozo, Silmara Silva, Wesley Viana, Marina Isaltino, Carolina Viana, Pedro Freitas, Diana Santana de Albuquerque","doi":"10.1016/j.joen.2025.01.022","DOIUrl":"10.1016/j.joen.2025.01.022","url":null,"abstract":"<p><strong>Introduction: </strong>The present study aimed to clinically compare the incidence of postoperative pain after endodontic treatment of posterior teeth using XP-endo Shaper (XPES; FKG Dentaire, La Chaux-de-Fons, Switzerland) and TruNatomy (TNY; Dentsply Sirona, Ballaigues, Switzerland) systems.</p><p><strong>Methods: </strong>In a single-blind randomized clinical trial, 104 vital teeth with an indication for conventional endodontic treatment for prosthetic purposes were treated by a single specialist following a pre-established protocol. All participants were unaware of the treatment they received. The teeth were randomly divided into 2 groups (n = 52) according to the instrumentation system used (TNY group and XPES group). The treatments were performed in a single visit. The participants were asked to rate the intensity of postoperative pain on a numeric rating scale and a visual analog scale (no pain, mild pain, moderate pain, and severe pain) after 24, 48, 72 h, and after 7 days.</p><p><strong>Results: </strong>The incidence of postoperative pain was zero in both groups at 48 h, 72 h, and 7 days. At 24 h, most patients in the XPES and TNY groups had no pain, while the remaining participants in either group experienced mild pain (1-3), with no significant difference between groups (P > .05, Mann-Whitney test).</p><p><strong>Conclusions: </strong>The instruments used in this study are associated with minimal postoperative pain, which only occurred in the first 24 h. Based on the parameters analyzed, we conclude that the systems tested cause mild postoperative pain over a short period of time.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Antimicrobial Peptide-loaded Hydrogels as Potential Scaffolds for Pulp-dentine Complex Regeneration: A Comparative Study.","authors":"Parisa Noohi, Mohammad J Abdekhodaie, Mohammad H Nekoofar, Miguel Gama, Maryam Saadatmand, Paul M H Dummer","doi":"10.1016/j.joen.2025.01.018","DOIUrl":"10.1016/j.joen.2025.01.018","url":null,"abstract":"<p><strong>Introduction: </strong>As a recently developed treatment modality, regeneration of the pulp-dentin-like complex via tissue engineering approaches is the focus of considerable attention. The persistence of bacteria within the root canal spaces is well known as the major challenge of dental pulp regeneration. Antimicrobial peptides (AMPs) are short peptides with inhibitory activities against a broad spectrum of microorganisms.</p><p><strong>Methods: </strong>In the present study, antimicrobial hydrogels based on 2 photo-reactive biopolymers, that is collagen methacrylate and chitosan methacrylate, and containing 2 different AMPs, that is LLKKK18 and Tet213, were used to develop a potential scaffold with antimicrobial and regenerative features.</p><p><strong>Results: </strong>The incorporation of AMPs had no significant effect on the physical, mechanical, rheological, and morphological properties of the new systems. The optimized concentration of LLKKK18 and Tet213 had appropriate antimicrobial activity against Gram-positive Enterococcus fæcalis and did not exhibit any cytotoxicity to stem cells from the apical papilla encapsulated within the hydrogels compared to the control group without AMPs. The presence of AMPs also significantly promoted migration and differentiation of stem cells from the apical papilla. In the AMP-loaded groups, greater levels of calcified nodules deposition, alkaline phosphatase, collagen type I, dentin sialophosphoprotein, dentin matrix protein 1, and vascular endothelial growth factor A mRNA gene expression, and collagen type I and dentin sialophosphoprotein protein expression were observed.</p><p><strong>Conclusions: </strong>The results indicated the potential of engineered antimicrobial collagen methacrylate/chitosan methacrylate hydrogels for application in tissue engineering-based strategies to regenerate the pulp-dentin complex.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}