Journal of endodontics最新文献

{"title":"Association between Apical Periodontitis and Autoimmune Diseases: A Systematic Review with Meta-analysis.","authors":"Tatiana A M do Nascimento, Francielle S Verner, Cleidiel Aparecido Araújo Lemos, Rafael B Junqueira","doi":"10.1016/j.joen.2025.01.021","DOIUrl":"10.1016/j.joen.2025.01.021","url":null,"abstract":"<p><strong>Objectives: </strong>The aim of this study was to perform a qualitative and quantitative synthesis of the available studies evaluating the possible relationship between apical periodontitis and autoimmune diseases.</p><p><strong>Methods: </strong>The literature search was conducted across 6 electronic databases (PubMed/MEDLINE, Embase, SciELO, Web of Science, Cochrane Library, and Scopus). The search covered articles published up to September 2023. There was no disagreement between the 2 reviewers. The RevMan software (V5.3) (Copenhagen: The Nordic Cochrane Centre, The Cochrane Collaboration, 2014) was used for the meta-analysis. A dichotomized analysis was performed using the Mantel-Haenszel statistical method with a fixed-effect model and the risk ratio as the effect measure. A 95% confidence interval was adopted, and the significance level was set at P < .05. The presence of apical periodontitis was predefined as the event, with the experimental group consisting of patients with autoimmune disease and the control group consisting of patients without autoimmune disease (healthy individuals). The data used included the number of individuals presenting the event vs the total number of study participants.</p><p><strong>Results: </strong>Nine studies were included and considered eligible. The methodological quality of the studies was classified as moderate in 5 of the 9 included studies, while 2 were rated as low, and 2 were rated as high-quality. The relative risk was 1.24 with a 95% confidence interval (1.08-1.62), in which the prevalence of apical periodontitis in comparing patients with autoimmune diseases and healthy patients yielded statistically significant results (P < .0001). Thus, patients with autoimmune diseases had a 24% higher relative risk of developing apical periodontitis compared to the healthy/control group.</p><p><strong>Conclusions: </strong>The analysis of the studies included in this review suggests a potential association between apical periodontitis and autoimmune diseases. However, it is important to consider that the selected studies have limitations, primarily because they are observational studies.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Novel Cryotherapy Technique on Postoperative Pain for Pulpotomy in Permanent Molars with Symptomatic Irreversible Pulpitis: A Randomized Controlled Trial.","authors":"Kübra Gürler, Koray Yilmaz","doi":"10.1016/j.joen.2025.01.020","DOIUrl":"10.1016/j.joen.2025.01.020","url":null,"abstract":"<p><strong>Introduction: </strong>The purpose of this study was to evaluate the effect of cryotherapy application on postoperative pain in permanent molars with symptomatic irreversible pulpitis after full pulpotomy procedures.</p><p><strong>Methods: </strong>Sixty-two patients with permanent maxillary and mandibular molars diagnosed with symptomatic irreversible pulpitis were included in the study. The teeth were randomly divided into 2 groups: the control group (n = 32) and the cryotherapy group (n = 30). In both groups, cotton pellets moistened with 2.5% sodium hypochlorite were used to achieve hemostasis. In the cryotherapy group, after hemostasis was achieved, the pulpotomy area was irrigated with saline solution at 2.5°C for 5 minutes. Then, in both groups, the pulp chamber was covered with Bio mineral trioxide aggregate+ (Cerkamed Company, Poland) and the composite restoration of the teeth (Clearfil Majesty Posterior, Kuraray Medical Inc., Tokyo, Japan) was completed. The participants were asked to rate the intensity of their postoperative pain via a Visual Analog Scale at 6, 12, 24, 48, and 72 hours. The data were analyzed using the Shapiro-Wilk, Levene's, Student t, Repeated Measures ANOVA and the Chi-square test.</p><p><strong>Results: </strong>The basic demographic data and analgesic intake of the 2 study groups were similar. Compared with those in the control group, the postoperative pain scores at 6 and 12 hours were significantly lower in the cryotherapy group (P < .05). The postoperative pain scores at 24, 48, and 72 hours were similar in both study groups.</p><p><strong>Conclusions: </strong>During vital pulp treatment, the cryotherapy application after hemostasis reduced postoperative pain in permanent molars with symptomatic irreversible pulpitis in the early period.</p>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255785","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracanal Antibacterial Effects of a Bioceramic Medication Compared With Calcium Hydroxide Pastes in Different Vehicles","authors":"Andrea F. Campello PhD ,&nbsp;Renata C.V. Rodrigues PhD ,&nbsp;Sabrina C. Brasil PhD ,&nbsp;Thais M. Souza Msc ,&nbsp;Flávio R.F. Alves PhD ,&nbsp;Ibrahimu Mdala MSc, PhD ,&nbsp;José F. Siqueira Jr. PhD ,&nbsp;Isabela N. Rôças PhD","doi":"10.1016/j.joen.2024.11.016","DOIUrl":"10.1016/j.joen.2024.11.016","url":null,"abstract":"<div><h3>Introduction</h3><div>The intracanal antibacterial effectiveness of a bioceramic medication was compared with calcium hydroxide pastes in different vehicles.</div></div><div><h3>Methods</h3><div>Extracted mandibular incisors with a single long oval canal were selected and distributed into 5 groups based on anatomically paired microcomputed tomographic analyses. The root canals were prepared up to an instrument size 35/04 and contaminated for 30 days with a mixed bacterial culture from subgingival biofilm added with <em>Enterococcus faecalis</em>. The canals were medicated with Bio-C Temp (Angelus Indústria de Produtos Odontológicos, Londrina, PR, Brazil) or a calcium hydroxide paste in glycerin, camphorated paramonochlorophenol and glycerin (CHPG), or 2% chlorhexidine (CHCX). Control specimens were filled with sterile saline. After 7 days, the medication was removed by using rotary instruments and saline irrigation. A supplementary approach was conducted, which consisted of 2.5% sodium hypochlorite irrigation and agitation with XP-endo Finisher. Bacteriological samples were taken before application (S1) and after removal (S2) of the intracanal medication, and after the supplementary approach (S3). Bacterial DNA extracted from the samples was quantified using real-time polymerase chain reaction.</div></div><div><h3>Results</h3><div>Bacterial counts were significantly reduced from S1 to S2 in all groups, except for the control group. As for S1-to-S3 changes, all groups (control included) showed significant bacterial reduction. S2-to-S3 changes were only significant in the CHPG and CHCX groups (<em>P</em> &lt; .05). Intergroup comparisons showed no significant differences in S2 (<em>P</em> &gt; .05), with all medications significantly better than the control (<em>P</em> &lt; .05). In S3, only the CHPG and CHCX groups showed further significant bacterial reductions. Categorical data analysis showed no intergroup differences (<em>P</em> &gt; .05).</div></div><div><h3>Conclusions</h3><div>All tested medications significantly reduced the intracanal bacterial counts, with no significant differences between them. Final sodium hypochlorite irrigation and agitation using XP-endo Finisher further enhanced disinfection in the CHCX and CHPG groups.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 207-212"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leukemia and Lymphoma Mimicking Periapical Conditions Resulting in Endodontic Treatment: A Systematic Review","authors":"Isis Samara de Melo Queiroga DDS ,&nbsp;Victor Zanetti Drumond DDS ,&nbsp;Lucas Guimarães Abreu DDS, MSc, PhD ,&nbsp;Lauren Frenzel Schuch DDS, MSc, PhD ,&nbsp;Ricardo Alves Mesquita DDS, MSc, PhD ,&nbsp;Erick Miranda Souza DDS, MSc, PhD ,&nbsp;Bruno Augusto Benevenuto de Andrade DDS, MSc, PhD ,&nbsp;José Alcides Almeida de Arruda DDS, MSc, PhD ,&nbsp;Gerhilde Callou Sampaio DDS, MSc, PhD","doi":"10.1016/j.joen.2024.11.005","DOIUrl":"10.1016/j.joen.2024.11.005","url":null,"abstract":"<div><h3>Introduction</h3><div>Leukemia and lymphoma mimicking periapical conditions can lead to significant diagnostic pitfalls. Guidance for endodontists in managing such cases is barely explored. This systematic review aimed to summarize the clinicopathologic, imaging, and management aspects of leukemia/lymphoma that mimicked periapical conditions and resulted in endodontic treatment.</div></div><div><h3>Methods</h3><div>Searches were conducted in PubMed, Embase, Scopus, Web of Science, and LILACS, supplemented by manual scrutiny and gray literature. Case reports and/or case series were included. Critical appraisal of the studies was performed using the Joanna Briggs Institute tool.</div></div><div><h3>Results</h3><div>Among 3,617 identified records, 32 studies involving 37 individuals (mean age 43.6 years; male-to-female ratio 1.2:1) were included. Diffuse large B-cell lymphoma was diagnosed in 21 (56.8%) individuals. The anterior maxilla and posterior mandible were the most frequently affected sites (29.7% each). Radiographically, 97.3% of the lesions exhibited radiolucency in the periapical region. Endodontic treatment preceded the diagnosis of leukemia/lymphoma in 94.6% of cases, while retreatment occurred in 5.4%. The mean time to final diagnosis was 4.9 months.</div></div><div><h3>Conclusion</h3><div>Leukemia and lymphoma can be misdiagnosed as periapical conditions, leading to inappropriate endodontic treatments and delayed diagnoses. Endodontists should become familiar with the broad clinicoradiographic spectrum of these rare, but potentially life-threatening hematolymphoid malignancies.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 106-117"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adrenomedullin-loaded Gelatin Methacryloyl Hydrogel Promotes Endogenous Dental Pulp Regeneration: An In Vitro and In Vivo Study","authors":"Yangpeng Zhao MD ,&nbsp;Qian Zhang MD ,&nbsp;Song Zhang MD ,&nbsp;Jianan Chen MD ,&nbsp;Lingtong Kong MD ,&nbsp;Jianyong Gao MD ,&nbsp;Qiang Zhu MD","doi":"10.1016/j.joen.2024.11.011","DOIUrl":"10.1016/j.joen.2024.11.011","url":null,"abstract":"<div><h3>Introduction</h3><div>To prepare a gelatin methacryloyl (GelMA) hydrogel scaffold embedded with adrenomedullin (ADM) and investigate its impact and underlying mechanisms in endogenous pulp regeneration.</div></div><div><h3>Methods</h3><div>ADM was evenly distributed within the GelMA hydrogel through a simple and conventional physical mixing technique. The scaffold underwent characterization via scanning electron microscopy, alongside assessments of swelling, degradation, and release properties. Biocompatibility was evaluated using cytoskeletal and live-dead staining techniques. The hydrogel's influence on dental pulp stem cells' proliferation, migration, and differentiation was assessed with CCK-8 assays, Transwell assays, and alizarin red and alkaline phosphatase staining. Transcriptomics provided insights into potential mechanisms. The angiogenic effects on umbilical vein endothelial cells were examined using scratch and tube formation assays. <em>In vivo</em>, the composite hydrogel's regenerative capacity was tested in a rat model of pulp regeneration. Statistical analysis involved Student's <em>t</em>-test and one-way analysis of variance, with significance set at <em>P</em> &lt; .05.</div></div><div><h3>Results</h3><div>The ADM-loaded GelMA (GelMA@ADM) hydrogel displayed a porous architecture under electron microscopy conducive to cell adhesion and demonstrated excellent biocompatibility. <em>In vitro</em> experiments showed that GelMA@ADM significantly boosted dental pulp stem cells' migration, proliferation, and differentiation, and enhanced the angiogenic activity of umbilical vein endothelial cells after one week of treatment. Corresponding <em>in vivo</em> experiments revealed that GelMA@ADM facilitated the formation of new vascularized pulp tissue after 2 weeks of treatment.</div></div><div><h3>Conclusions</h3><div>The GelMA@ADM hydrogel effectively promotes dental pulp stem cells' proliferation and differentiation, augments vascularization by umbilical vein endothelial cells, and fosters the creation of new vascularized pulp tissue. These findings underscore the potential of GelMA@ADM hydrogel for endogenous pulp regeneration.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 172-184"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Debris Apical Extrusion, Unprepared Surface Areas, and Dentinal Crack Formation after Root Canal Preparation Using ProTaper Next, ProTaper Ultimate, and R-Motion File Systems","authors":"Haider A. Ibrahim BDS ,&nbsp;Ahmed H. Ali BDS, MSc, PhD (UK) ,&nbsp;Francesco Mannocci MD, DDS, PhD, FHEA","doi":"10.1016/j.joen.2024.11.014","DOIUrl":"10.1016/j.joen.2024.11.014","url":null,"abstract":"<div><h3>Introduction</h3><div>This study evaluated and compared the amount of apically extruded debris, unprepared wall surface areas, and dentinal crack formation resulting from root canal preparation using ProTaper Next (PTN), ProTaper Ultimate (PTU), and R-Motion (RM) file systems.</div></div><div><h3>Methods</h3><div>Forty-five palatal roots of maxillary first molars were shortened to a uniform length of 12 mm. The samples were divided randomly into 3 groups (<em>n</em> = 15/group) to be prepared using either PTN, PTU, and RM file systems. The investigator adopted modified Myers and Montgomery's approach to replicate the temperature of the human body. Debris was collected in vials and weighed before and after instrumentation with a sensitive balance. Every specimen was irrigated using distilled water during preparation, utilizing a side-vented needle. Weight before instrumentation was subtracted from weight after instrumentation to identify the weight of the collected debris. Cone-beam computed tomography (CBCT) scans were taken before and after canal preparation using a Planmeca ProMax CBCT machine. The unprepared canal surface area was calculated by superimposing preoperative and postoperative CBCT images using the Mimics Medical program. After preparation, a low-speed diamond disc horizontally sectioned the roots at 3, 6, and 9 millimeters from the apex. The specimens were examined under a digital microscope to detect crack formation. Statistical analysis was carried out using one-way (ANOVA) and Tukey (HSD) tests, with a significance level of <em>P</em> &lt; .05.</div></div><div><h3>Results</h3><div>There was no statistically significant difference in the quantity of debris extruded apically and dentinal crack numbers among the 3 shaping files (<em>P</em> &gt; .05). Only the apical third of root canals prepared by PTU displayed a significantly lower unprepared surface area compared to PTN and RM.</div></div><div><h3>Conclusions</h3><div>Instrumentation with PTN, PTU, and RM yielded similar amounts of apically extruded debris and dentinal cracks. The PTU files demonstrated higher effectiveness in preparing the apical third by reducing the unprepared surface area in the prepared root canal.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 200-206"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Apical Periodontitis with Glycated Hemoglobin Levels and Systemic Inflammatory Markers in Patients with Type 2 Diabetes: A Cross-Sectional Study","authors":"Ritika Dhamija MDS ,&nbsp;Sanjay Tewari MDS ,&nbsp;Paramjeet Singh Gill MD ,&nbsp;Nitika Monga MDS ,&nbsp;Shweta Mittal MDS ,&nbsp;Jigyasa Duhan MDS","doi":"10.1016/j.joen.2024.11.008","DOIUrl":"10.1016/j.joen.2024.11.008","url":null,"abstract":"<div><h3>Introduction</h3><div>This cross-sectional study, as a preliminary part of an ongoing project, aimed to investigate the effect of apical peridontitis (AP) on glycated hemoglobin (HbA1c) and systemic inflammatory markers in diabetic individuals.</div></div><div><h3>Methods</h3><div>A total of 280 individuals (140 with type 2 diabetes mellitus [T2DM] and 140 healthy) with and without AP were enrolled. Sixty-four age-, gender-, and body mass index–matched participants each in T2DM with AP group (DAP), T2DM without apical periodontitis (D), systemically healthy controls with apical periodontitis (CAP), and without apical periodontitis (C) groups were evaluated. Radiologic and clinical oral examination was performed for confirming the diagnosis of AP and periapical index scoring (PAI). Blood analyses were carried out for interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, high-sensitivity C-reactive protein (hsCRP), and HbA1c assessment.</div></div><div><h3>Results</h3><div>Significantly higher levels of IL-1β, TNF-α, and hsCRP were observed in patients with AP in both diabetes and control groups (<em>P</em> &lt; .05). In the diabetes group, AP contributed to significantly raised levels of HbA1c compared with T2DM group patients without AP. After controlling for possible confounders, partial corelation coefficients revealed positive corelation of presence of AP as well as size of the periapical lesion with HbA1c and serum levels of inflammatory markers in both diabetic and healthy individuals. Multivariate linear regression analysis revealed both presence of AP (<em>P</em> &lt; .05) as well as the size of lesion (<em>P</em> &lt; .001) were found to significantly predict the HbA1c levels as well as the levels of IL-1β, IL-6, TNF-α, and hsCRP in both diabetic and non-diabetic individuals.</div></div><div><h3>Conclusions</h3><div>These findings suggest that both presence of AP and size of periapical lesion was associated with glycemic control and systemic inflammatory burden in patients with T2DM.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 124-131"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142710289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into the February 2025 Issue of the JOE","authors":"Anita Aminoshariae DDS, MS,&nbsp;Amir Azarpazhooh DDS, MSc, PhD, FRCD(C),&nbsp;Gerald N. Glickman DDS, MS, MBA, JD,&nbsp;Jianing He DMD, PhD,&nbsp;Sahng G. Kim DDS, MS,&nbsp;Anil Kishen BDS, MDS, PhD,&nbsp;Ariadne M. Letra DDS, MS, PhD,&nbsp;Linda Levin DDS, PhD,&nbsp;Ronald Ordinola-Zapata,&nbsp;Frank C. Setzer DMD, PhD, MS,&nbsp;Franklin R. Tay BDSc(Hons), PhD,&nbsp;Kenneth M. Hargreaves DDS, PhD","doi":"10.1016/j.joen.2025.01.017","DOIUrl":"10.1016/j.joen.2025.01.017","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 103-105"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143255786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peripheral Lysosomal Positioning in Inflamed Odontoblasts Facilitates Mineralization","authors":"Nuo Xu DDS,&nbsp;Qian Gao DDS,&nbsp;Chengcan Yang DDS, PhD,&nbsp;Xiaona Song BDS,&nbsp;Kai Yang DDS, PhD,&nbsp;Zhuan Bian DDS, PhD","doi":"10.1016/j.joen.2024.11.006","DOIUrl":"10.1016/j.joen.2024.11.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Odontoblasts, terminally differentiated dentin-producing cells, critically rely on lysosomal functions for intracellular recycling and renewal. Beyond their traditional degradative role, lysosomes actively orchestrate cellular responses to external stimuli through precise and rapid intracellular trafficking and positioning. This study aimed to explore the influence of lysosomal positioning on odontoblast mineralization and the underlying mechanisms implicated in carious inflammation.</div></div><div><h3>Methods</h3><div>Human dental pulp stem cells were induced to differentiate into human odontoblast-like cells (hOBLCs). hOBLCs were treated with various doses of LPS (0.1, 1, 5 μg/mL) to mimic carious inflammation. Lysosomal positioning was examined by immunofluorescence staining of lysosomal associated membrane protein 1 in healthy and carious human teeth, LPS-treated hOBLCs, mouse lower incisors at postnatal day 2.5, and mineralization medium cultured human dental pulp stem cells. Lysosomal positioning was manipulated by knockdown or overexpression of <em>SNAPIN</em> or <em>ARL8B</em>. Mineralization was assessed by ARS staining and expression of DSPP and DMP1. Lysosomal exocytosis was examined by detection of lysosomal-plasma membrane fusion, surface exposure of lysosomal associated membrane protein 1 luminal epitopes (1D4B), and extracellularly released lysosomal enzymes.</div></div><div><h3>Results</h3><div>Peripheral lysosomal positioning was markedly increased in odontoblasts within moderate and extensive carious lesions (<em>P</em> &lt; .001) and in hOBLCs following LPS treatment. Increased peripheral dispersion of lysosomes was similarly observed during odontoblastic differentiation <em>in vivo</em> and <em>in vitro</em>. Moreover, peripheral lysosomal positioning promoted mineralization in inflamed hOBLCs, potentially via mTORC1 signaling pathway and lysosomal exocytosis.</div></div><div><h3>Conclusion</h3><div>Inflammatory stimuli prompted a relocation of lysosomes in odontoblasts, redistributing them from perinuclear location toward the cell periphery, which in turn facilitated mineralization, potentially via mTORC1 signaling and lysosomal exocytosis.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 185-194"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlling Root Canal Infection in Oncological Patients with Apical Periodontitis","authors":"Kaline Romeiro PhD ,&nbsp;Luciana F. Gominho PhD ,&nbsp;Danielle D. Voigt PhD ,&nbsp;Ibrahimu Mdala PhD ,&nbsp;Isabela N. Rôças PhD ,&nbsp;José F. Siqueira Jr. PhD","doi":"10.1016/j.joen.2024.11.012","DOIUrl":"10.1016/j.joen.2024.11.012","url":null,"abstract":"<div><h3>Introduction</h3><div>This study evaluated the effectiveness of chemomechanical preparation associated with intracanal medication in reducing the total bacterial load of infected root canals in cancer patients undergoing antineoplastic treatment. The prevalence, levels, and response to treatment of <em>Streptococcus</em> and Actinobacteria species were also assessed.</div></div><div><h3>Methods</h3><div>The root canals of teeth with apical periodontitis from oncological and control patients were treated using rotary instrumentation, 2.5% NaOCl irrigation, and 2-week calcium hydroxide medication (<em>n</em> = 23/group). Bacteriological root canal samples were taken before (S1) and after chemomechanical preparation (S2), and after intracanal medication (S3). DNA extracted from the samples was subjected to 16S rRNA gene-based quantitative real-time polymerase chain reaction assays for evaluation of the treatment effects on total bacteria, <em>Streptococcus</em> species, and members of the Actinobacteria phylum.</div></div><div><h3>Results</h3><div>All S1 samples were positive for bacteria. S1–S2 and S1–S3 bacterial reduction values were highly significant (<em>P</em> &lt; .01). Although bacterial counts were reduced from S2 to S3, the decrease was not statistically significant (<em>P</em> &gt; .05). Intergroup comparisons showed no significant differences in S1, S2 and S3 counts (<em>P</em> &gt; .05). In the oncological group, 77% of the cases were positive for bacteria in S2, and 68% in S3. In the control group, corresponding figures were 65% for both S2 and S3. There were no significant differences between groups as for streptococci and actinobacteria prevalence, levels, and response to treatment (<em>P</em> &gt; .05).</div></div><div><h3>Conclusions</h3><div>Root canal treatment using rotary instruments, NaOCl irrigation, and calcium hydroxide medication was highly effective in reducing the bacterial populations in infected teeth with apical periodontitis from cancer patients, with no significant differences to controls.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"51 2","pages":"Pages 157-163"},"PeriodicalIF":3.5,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}