Journal of endodontics最新文献

{"title":"The OHStat Guidelines for Reporting Observational Studies and Clinical Trials in Oral Health Research: Manuscript Checklist","authors":"A.M. Best ,&nbsp;T.A. Lang ,&nbsp;B.L. Greenberg ,&nbsp;J.C. Gunsolley ,&nbsp;E. Ioannidou ,&nbsp;Task Force on Design and Analysis in Oral Health Research","doi":"10.1016/j.joen.2024.05.007","DOIUrl":"10.1016/j.joen.2024.05.007","url":null,"abstract":"<div><div>Adequate and transparent reporting is necessary for critically appraising published research. Yet, ample evidence suggests that the design, conduct, analysis, interpretation, and reporting of oral health research could be greatly improved. Accordingly, the Task Force on Design and Analysis in Oral Health Research—statisticians and trialists from academia and industry—identified the minimum information needed to report and evaluate observational studies and clinical trials in oral health: the OHStat Guidelines. Drafts were circulated to the editors of 85 oral health journals and to Task Force members and sponsors and discussed at a December 2020 workshop attended by 49 researchers. The guidelines were subsequently revised by the Task Force’s writing group. The guidelines draw heavily from the Consolidated Standards for Reporting Trials (CONSORT), Strengthening the Reporting of Observational Studies in Epidemiology (STROBE), and CONSORT harms guidelines and incorporate the SAMPL guidelines for reporting statistics, the CLIP principles for documenting images, and the GRADE indicating the quality of evidence. The guidelines also recommend reporting estimates in clinically meaningful units using confidence intervals, rather than relying on <em>P</em> values. In addition, OHStat introduces 7 new guidelines that concern the text itself, such as checking the congruence between abstract and text, structuring the discussion, and listing conclusions to make them more specific. OHStat does not replace other reporting guidelines; it incorporates those most relevant to dental research into a single document. Manuscripts using the OHStat guidelines will provide more information specific to oral health research.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1655-1661"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Teaching a New Obturation Technique in Preclinical Endodontic Training: Assessment of Student Learning Experience, Performance, and Self-Evaluation","authors":"Meltem Kucuk DDS, PhD ,&nbsp;Mohini Ratakonda BDS, MDS ,&nbsp;Lucila Piasecki DDS, PhD, MS, BDS","doi":"10.1016/j.joen.2024.09.004","DOIUrl":"10.1016/j.joen.2024.09.004","url":null,"abstract":"<div><h3>Introduction</h3><div>This study investigated the impact of implementing hydraulic condensation (HC) as a second obturation technique in the Endodontic Preclinical course for dental students trained in warm vertical compaction (WVC).</div></div><div><h3>Methods</h3><div>A total of 70 students performed root canal treatment of 2 extracted molars; they performed the HC technique for the first time after a demonstration. The combination of the obturation technique (WVC or HC) and the type of molar (maxillary or mandibular) was randomly assigned, resulting in 4 groups (<em>n</em> = 35). The students answered a questionnaire appraising their learning experience and completed a self-evaluation rubric. Masked instructors graded projects. Data were statistically analyzed (significance set at 5%).</div></div><div><h3>Results</h3><div>Most of the students (91%) considered learning 2 obturation techniques relevant and considered it difficult to perform the downpack in molars (<em>P</em> &lt; .05). About half of the students (45.7%) responded that HC resulted in fewer errors and disagreed that WVC derives in a more homogeneous obturation; accordingly, 52.9% of the students selected the highest self-grading for HC obturations, while only 38.6% for WVC. No significant difference was found in the distribution of grades attributed by students and instructors (<em>P</em> &gt; .05). Students reported significantly higher levels of confidence for HC and difficulty for WVC (<em>P</em> &lt; .05). No difference was found between the techniques regarding the presence of voids. The presence of multiple errors was more frequent for WVC (<em>P</em> &lt; .05).</div></div><div><h3>Conclusion</h3><div>The findings indicate a positive learning experience and comparable performance among preclinical dental students in implementing HC as an alternative obturation technique.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1634-1641"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights Into the November 2024 Issue of the Journal of Endodontics","authors":"Anita Aminoshariae DDS, MS,&nbsp;Amir Azarpazhooh DDS, MSc, PhD, FRCD(C),&nbsp;Ashraf F. Fouad DDS, MS,&nbsp;Gerald N. Glickman DDS, MS, MBA, JD,&nbsp;Jianing He DMD, PhD,&nbsp;Sahng G. Kim DDS, MS,&nbsp;Anil Kishen BDS, MDS, PhD,&nbsp;Ariadne M. Letra DDS, MS, PhD,&nbsp;Linda Levin DDS, PhD,&nbsp;Frank C. Setzer DMD, PhD, MS,&nbsp;Franklin R. Tay BDSc (Hons), PhD,&nbsp;Kenneth M. Hargreaves DDS, PhD","doi":"10.1016/j.joen.2024.10.007","DOIUrl":"10.1016/j.joen.2024.10.007","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1539-1541"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaphylaxis after Treatment with an Endodontic Material Containing Polyethylene Glycol","authors":"Marie Alnæs MD ,&nbsp;Torgeir Storaas MD, PhD ,&nbsp;Hilde Kristin Vindenes MD, PhD ,&nbsp;Anne Berit Guttormsen MD ,&nbsp;Lars Björkman DDS, PhD","doi":"10.1016/j.joen.2024.09.002","DOIUrl":"10.1016/j.joen.2024.09.002","url":null,"abstract":"<div><div>Polyethylene glycol (PEG) is sometimes included in temporary endodontic filling materials to modify viscosity. PEG is considered a low hazard chemical, but parenteral PEG exposure is associated with a risk of anaphylaxis. We report 2 cases of anaphylaxis after treatment with an endodontic temporary filling material containing PEG. One patient survived after hospital treatment, but the second case was fatal. Extensive investigations were needed to find the culprit. Allergic reactions to PEG are increasingly reported and have significant implications for the patient. Precautionary measures are discussed.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1649-1654"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proinflammatory Effect of Membrane Vesicles Derived from Enterococcus faecalis at Neutral and Alkaline pH","authors":"Chenguang Niu ,&nbsp;Jing Huang ,&nbsp;Lifan Wei ,&nbsp;Jia Wang ,&nbsp;Shujun Ran","doi":"10.1016/j.joen.2024.08.018","DOIUrl":"10.1016/j.joen.2024.08.018","url":null,"abstract":"<div><h3>Introduction</h3><div>The present study explored the proinflammatory impact of <em>Enterococcus faecalis</em> membrane vesicles (MVs) derived from culture medium at pH levels of 7.4 and 9.0.</div></div><div><h3>Methods</h3><div><em>E. faecalis</em> MVs were obtained by centrifugation and purified by size-exclusion chromatography. Proteomic analyses were performed on <em>E. faecalis</em> MVs to investigate their components. THP-1 macrophages were exposed to <em>E. faecalis</em> MVs, and the inflammatory cytokines and proteins were evaluated using enzyme-linked immunosorbent assay and immunoblotting. The inflammatory cytokines in the serum of mice with intraperitoneal injection of <em>E. faecalis</em> MVs were evaluated by enzyme-linked immunosorbent assay, and immunophenotyping of spleen cells was investigated with flow cytometry.</div></div><div><h3>Results</h3><div>Proteomic analysis revealed 196 proteins in <em>E. faecalis</em> MVs obtained under neutral and alkali conditions; 110 proteins were up-regulated, and 79 proteins were down-regulated by alkaline pH. <em>E. faecalis</em> MVs induced secretion of inflammatory factors interleukin (IL)-1β, IL-6, and tumor necrosis factor alpha in a concentration-dependent manner. Immunoblotting revealed that <em>E. faecalis</em> MVs increased expression of pro–IL-1β, nuclear factor kappa Bp65, and Toll-like receptor 2. <em>In vivo</em> studies demonstrated that <em>E. faecalis</em> MVs significantly promoted secretion of IL-1β in mouse serum, whereas inflammatory cells were activated in the spleen. <em>E. faecalis</em> MVs obtained at a pH of 9.0 showed stronger proinflammatory effects than those obtained under neutral pH.</div></div><div><h3>Conclusions</h3><div><em>E. faecalis</em> produces MVs that carry specific proteins associated with virulence factors, and these MVs can promote inflammation <em>in vitro</em> and <em>in vivo. E. faecalis</em> MVs obtained under alkaline conditions have a stronger proinflammatory effect.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1602-1611.e10"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107984","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Immunomodulatory Nanoparticles Inhibit Root Resorption and Ankylosis","authors":"Rajeshwari Hadagalu Revana Siddappa BDS, MDS, PhD ,&nbsp;Emily Bishop DDS, MSc ,&nbsp;Aiman Ali DDS, PhD ,&nbsp;Marco Magalhaes DDS, MSc, PhD ,&nbsp;Anil Kishen BDS, MDS, PhD","doi":"10.1016/j.joen.2024.08.006","DOIUrl":"10.1016/j.joen.2024.08.006","url":null,"abstract":"<div><h3>Introduction</h3><div>External root resorption following avulsion injury is a complex process wherein differentiation of macrophages (Mϕ) to multinucleated osteoclasts is temporally regulated by resident periodontal fibroblasts (PDLF). The current study aims to assess the effect of engineered bioactive chitosan nanoparticles (CSNP), sustained released dexamethasone conjugated CSNP (CS-DEX) and CSNP functionalized with photosensitizer Rose Bengal (CSRB) for application in root resorption using an <em>in-vitro</em> PDLF-Mϕ direct coculture model and <em>in-vivo</em> delayed reimplantation model.</div></div><div><h3>Methods</h3><div>PDLF-Mϕ direct coculture system was exposed to lipopolysaccharide (LPS), macrophage colony stimulating factor, receptor activator of nuclear factor kappa β ligand with or without CSNP/CS-DEX for 7 days. Clastic differentiation was assessed by tartrate resistant acid phosphatase (TRAP) staining on day 7. On day 2 and 7, immunofluorescence analysis was conducted to assess the expression of Mϕ polarization markers (CD80, CD206), multinucleation markers (NFATc1, STAT6) in Mϕ and matricellular protein periostin in PDLF and cytokine profiling in cell culture supernatants. Delayed replantation model with extraoral air dry/LPS exposure for 1h followed by root surface treatment with CS-DEX/CSRB was used in Wistar rats. After 21 days, rats were euthanized for histologic and immunofluorescence analysis. Statistical analysis one-way ANOVA with Tukey's multiple comparisons was used to analyze the data (<em>P</em> &lt; .05).</div></div><div><h3>Results</h3><div>CS-DEX significantly reduced TRAP⁺ multinucleated cells and CSNP treatment showed no TRAP⁺ cells. Immunofluorescence analysis showed that CSNP/CS-DEX reduced CD80, NFATc1 and STAT6 expression and increased periostin as expressed by fluorescence intensity. CSNP/CS-DEX significantly reduced TNFα, MMP9 and increased IL10, TGFβ1. Osteoprotegerin was upregulated only by CSNP. Root surface treatment in delayed replantation model showed that CS-DEX and CSRB substantially reduced the degree of resorption and ankylosis. Further, CD80, CD206, and MMP2 expression in groups with root surface treatment with CS-DEX and CSRB was lower than airdry/LPS group and similar to healthy control and NFATc1, STAT6, and MMP9 expressions were lower than healthy control.</div></div><div><h3>Conclusion</h3><div>The engineered nanosized immunomodulatory bioactive materials chitosan nanoparticles functionalized with photosensitizer and dexamethasone effectively reduced the clastic differentiation of Mϕ in <em>in-vitro</em> coculture and minimized the resorption and ankylosis in a delayed reimplantation model. These biomaterials have the potential to serve as root modification agents, promoting favorable healing outcomes in cases of dental avulsion.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1579-1592.e3"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Guidelines for Authors","authors":"","doi":"10.1016/S0099-2399(24)00566-1","DOIUrl":"10.1016/S0099-2399(24)00566-1","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages A12-A17"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143148029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to Letter From Savadori","authors":"","doi":"10.1016/j.joen.2024.08.001","DOIUrl":"10.1016/j.joen.2024.08.001","url":null,"abstract":"","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Page 1542"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Outcome of Nonsurgical Root Canal Retreatment of Teeth with Persistent Apical Periodontitis Treated with Foraminal Enlargement and 2% Chlorhexidine Gel: A Retrospective Cohort Study","authors":"Patrick Wilson Quelis Baltieri DDS, MSc, PhD ,&nbsp;Lucas Peixoto de Araújo DDS, MSc, PhD ,&nbsp;Brenda P.F.A. Gomes DDS, MSc, PhD ,&nbsp;José Flávio Affonso de Almeida DDS, MSc, PhD ,&nbsp;Caio Cezar Randi Ferraz DDS, MSc, PhD ,&nbsp;Adriana de-Jesus-Soares DDS, MSc, PhD","doi":"10.1016/j.joen.2024.09.006","DOIUrl":"10.1016/j.joen.2024.09.006","url":null,"abstract":"<div><h3>Introduction</h3><div>Nonsurgical root canal retreatment (NS-RCRT) becomes necessary when primary endodontic procedures fail. This study evaluates the efficacy of NS-RCRT using 2% chlorhexidine gel and foraminal enlargement techniques, aiming to assess whether these approaches enhance periapical healing outcomes and success rates compared to traditional NS-RCRT techniques reported in the literature.</div></div><div><h3>Methods</h3><div>This retrospective cohort study analyzed 120 teeth diagnosed with persistent apical periodontitis, from 80 patients who underwent NS-RCRT between January 2014 and December 2018 at a specialist's private practice. Data were collected following the Preferred Reporting Items for Observational Studies in Endodontics 2023 guidelines. Periapical healing was evaluated using digital periapical radiographs by three calibrated examiners. The outcome of the treatment was analyzed through descriptive statistics and bivariate analyses, including the Chi-Square and Fisher's Exact tests. Treatment outcomes were deemed successful if they showed complete or incomplete repair and unsuccessful if no repair was observed.</div></div><div><h3>Results</h3><div>The average follow-up period was 30 months. Under loose criteria, 92.50% (<em>n</em> = 111) of the teeth were categorized as successful, and 7.5% (<em>n</em> = 9) as unsuccessful. Bivariate analysis indicated that the radiographic restoration of apical transportation was the only factor that significantly influenced the outcome.</div></div><div><h3>Conclusions</h3><div>NS-RCRT performed in a single visit using the foraminal enlargement technique and 2% chlorhexidine gel demonstrated high success rates and may be an effective alternative to tooth extraction. This method promoted periapical healing and could significantly improve NS-RCRT protocols. Further prospective studies are recommended to corroborate these findings.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1551-1559"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142307866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Invasive Oral Squamous Cell Carcinoma Mimicking a Dentoalveolar Abscess: Report of a Case in a 14-Year-Old Patient","authors":"Mark D. Stevenson DDS ,&nbsp;Craig A. Dunlap DDS ,&nbsp;Michelle Shin DDS ,&nbsp;Shreyas Oza BDS, DDS, MSD ,&nbsp;Gordon S. Lai DDS, MSD","doi":"10.1016/j.joen.2024.05.011","DOIUrl":"10.1016/j.joen.2024.05.011","url":null,"abstract":"<div><h3>Introduction</h3><div><span>A diagnosis of oral squamous cell carcinoma in adolescent patients is extremely rare. When an oral squamous cell carcinoma lesion arises near the teeth and/or periodontium, it can be easily misdiagnosed as an inflammatory condition of </span>endodontic or periodontal origin.</div></div><div><h3>Methods</h3><div>This is a case report of an otherwise healthy 14-year-old patient who was referred for endodontic evaluation and treatment of a soft-tissue swelling in the anterior maxilla.</div></div><div><h3>Results</h3><div>The unexpected definitive diagnosis of invasive oral squamous cell carcinoma underscores the importance of proper diagnostic testing.</div></div><div><h3>Conclusions</h3><div>Accurate interpretation of pulp testing results, periapical and cone beam computed tomography imaging, timely biopsy, and prompt definitive treatment are critical when a lesion of nonodontogenic origin is suspected.</div></div>","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":"50 11","pages":"Pages 1642-1648"},"PeriodicalIF":3.5,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}