Journal of endodontics最新文献_第10页

Prevalence of Vertical Root Fractures in Root Filled Teeth and Their Clinical and Radiographic Risk Factors: A Modern Case-Control Analysis Based on Surgical Diagnosis. 根充填牙纵根骨折的患病率及其临床和影像学危险因素：基于外科诊断的现代病例对照分析。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-05 DOI: 10.1016/j.joen.2025.07.020

Phachara Promchouy, Kanet Chotvorrarak, Sittichoke Osiri

{"title":"Prevalence of Vertical Root Fractures in Root Filled Teeth and Their Clinical and Radiographic Risk Factors: A Modern Case-Control Analysis Based on Surgical Diagnosis.","authors":"Phachara Promchouy, Kanet Chotvorrarak, Sittichoke Osiri","doi":"10.1016/j.joen.2025.07.020","DOIUrl":"10.1016/j.joen.2025.07.020","url":null,"abstract":"Introduction: Vertical root fractures (VRFs) are challenging in endodontic diagnosis. This study determined VRF prevalence and risk factors in root-filled teeth (RFT) requiring endodontic surgery.Methods: From 411 RFT that underwent endodontic surgery (2005-2022), 130 VRF cases and 260 randomly selected controls were analyzed. Demographic, clinical, and radiographic data were collected from dental records and images. Multiple imputations addressed missing data. Univariable and multivariable logistic regression analyses with robust standard errors to account for the clustering of multiple teeth within the same patient were performed, with subanalyses for teeth with intraradicular posts and molars. VRF characteristics were analyzed in individuals with available cone beam computed tomography images.Results: VRF prevalence was 31.63% among RFT undergoing endodontic surgery. Significant risk factors included age (adjusted odds ratio [aOR] = 1.02), probing depth ≥5 mm (aOR = 2.37), isolated perilateral radiolucency (aOR = 4.05), halo radiolucency (aOR = 2.70), overfilled root canal (aOR = 2.11), and root canal space-to-root-width ratio >1/3 (aOR = 1.52). For teeth with posts, a post space-to-root-width ratio >1/3 increased VRF risk (unadjusted OR [uOR] = 2.46). Cone beam computed tomography findings revealed the absence of the buccal plate (uOR = 1.74) and space between the cortical plate and root surface (uOR = 2.73) as significant factors.Conclusions: VRFs affected nearly one-third of RFT requiring endodontic surgery; major risk factors included older age, overfilled root canals, and excessive root canal space-to-root-width ratio. VRF-associated clinical and radiographic signs included probing depth ≥5 mm, isolated perilateral radiolucency, and halo radiolucency.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144794661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autonomous Robot-Assisted Endodontic Microsurgery on First Molar in Complex Anatomical Scenarios: A Case Series. 在复杂解剖情况下自主机器人辅助第一磨牙根管显微手术：一个案例系列。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-09 DOI: 10.1016/j.joen.2025.04.017

Lishan Huang, Minting Wan, Xiaoxing Li, Siyu Li, Qingsong Wu, Chengji Gong, Yufei Li, Xuechao Yang

{"title":"Autonomous Robot-Assisted Endodontic Microsurgery on First Molar in Complex Anatomical Scenarios: A Case Series.","authors":"Lishan Huang, Minting Wan, Xiaoxing Li, Siyu Li, Qingsong Wu, Chengji Gong, Yufei Li, Xuechao Yang","doi":"10.1016/j.joen.2025.04.017","DOIUrl":"10.1016/j.joen.2025.04.017","url":null,"abstract":"Endodontic microsurgery (EMS) is an important approach for preserving teeth with failed root canal treatment. However, implementing minimally invasive osteotomies and accurate apicoectomies can be challenging in anatomically complex areas, especially posterior teeth close to critical structures. The introduction of autonomous robotic system into EMS represents a pioneering advancement aimed at enhancing digital endodontic surgery. Robot-assisted EMS (RA-EMS) integrates cone-beam computed tomography and intraoral optical scanning data to plan three-dimensional surgical paths, adopting infrared optical motion-tracking technology and robot autonomous drive, enabling precise apicoectomy. This report presents the successful application of RA-EMS in 4 complex molar cases, during which adjacent vital anatomical structures were preserved. Immediate radiographic examination revealed accurate root-end resections. All patients demonstrated satisfactory healing during the follow-up visits at 1-6 months, with periapical radiolucent area decreased in radiographic examination. RA-EMS is a safe and feasible clinical procedure that minimizes the risk of human error and mitigates technique sensitivity. Meanwhile, it provides a valuable alternative for assisting anatomically challenging EMS.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"1118-1126"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144021400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Jasminum-based Nano-reinforced Calcium Hydroxide Reduces Postoperative Pain in Symptomatic Apical Periodontitis: A Randomized Controlled Trial. 茉莉基纳米增强氢氧化钙减轻症状性根尖牙周炎术后疼痛：一项随机对照试验。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-06-02 DOI: 10.1016/j.joen.2025.05.016

Nehal Amir, Emaan Mansoor, Nabiha Eeman, Muhammad Nouman Ahmed, Ezza Mansoor, Efrah Mansoor, Khadim Hussain, Vera Afreixo, Afsheen Mansoor, João Filipe Brochado Martins, Paulo J Palma

{"title":"Jasminum-based Nano-reinforced Calcium Hydroxide Reduces Postoperative Pain in Symptomatic Apical Periodontitis: A Randomized Controlled Trial.","authors":"Nehal Amir, Emaan Mansoor, Nabiha Eeman, Muhammad Nouman Ahmed, Ezza Mansoor, Efrah Mansoor, Khadim Hussain, Vera Afreixo, Afsheen Mansoor, João Filipe Brochado Martins, Paulo J Palma","doi":"10.1016/j.joen.2025.05.016","DOIUrl":"10.1016/j.joen.2025.05.016","url":null,"abstract":"Introduction: This study evaluated the efficacy of a novel Jasminum-based titanium dioxide nano-reinforced calcium hydroxide (Ca(OH)2) intracanal medicament in reducing postoperative pain (PP) compared to conventional Ca(OH)2 in patients with symptomatic apical periodontitis.Methods: A triple-blind randomized clinical trial, registered at ClinicalTrials.gov (NCT06653608, 10/19/2024) included patients with single-rooted teeth diagnosed with pulp necrosis and symptomatic AP. Patients referred for endodontic treatment fulfilling the inclusion criteria were included. The patients were grouped into nano-reinforced Ca(OH)2 group (n = 30) and conventional-Ca(OH)2 (n = 30). Preoperative pain and PP at 4, 24, 48, 72, and 96 hours were recorded using a visual analog scale. Data were analyzed using linear mixed-effects models (R software, version 4.3.2., R Core Team, Vienna, Austria), with significance set at P < .05.Results: Sixty patients with comparable demographic and clinical characteristics, including preoperative pain scores, were recruited. Both groups exhibited significant pain reduction over 96 hours (P < .001). However, a significant group-time interaction was observed (P < .001), with the nano-reinforced Ca(OH)2 group reporting consistently lower PP scores and greater reductions in PP compared to the conventional Ca(OH)2 group at all-time points.Conclusion: The incorporation of Jasminum-based TiO2 nanoparticles to Ca(OH)2 significantly improved the reduction in PP intensity and duration compared to conventional Ca(OH)2, making it a superior option for PP management.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"996-1005"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144225667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Micro-computed Tomographic Analysis of the Efficacy of 3 Techniques for Removal of Calcium Hydroxide from the Mesial Canals of Curved Mandibular Molars. 三种方法去除弯曲下颌磨牙近中管氢氧化钙的显微计算机断层分析。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-29 DOI: 10.1016/j.joen.2025.05.013

Jenna Gaudio Edwards, John Nusstein, Melissa Drum, Sara Fowler, Al Reader

{"title":"Micro-computed Tomographic Analysis of the Efficacy of 3 Techniques for Removal of Calcium Hydroxide from the Mesial Canals of Curved Mandibular Molars.","authors":"Jenna Gaudio Edwards, John Nusstein, Melissa Drum, Sara Fowler, Al Reader","doi":"10.1016/j.joen.2025.05.013","DOIUrl":"10.1016/j.joen.2025.05.013","url":null,"abstract":"Introduction: The purpose of this study was to evaluate the efficacy of 3 irrigation techniques: standard syringe irrigation (SI), ProUltra PiezoFlow (CUI), and EdgeEndo Er,Cr:YSGG (LAI) laser in removing calcium hydroxide from the mesial canals of severely curved mandibular molars using micro-computed tomography (micro-CT) analysis.Methods: Mesial canals of 30 extracted human mandibular molar teeth were prepared to size 30/.04 taper or 35/.04 taper rotary instruments. Teeth were randomly assigned to the 3 experimental groups (n = 10) with evenly distributed degrees of curvature. Calcium hydroxide was placed into prepared canals and sealed for 14 days. A micro-CT scan was taken to determine calcium hydroxide paste volume in each tooth. Canals were irrigated utilizing the 3 techniques and re-scanned. Micro-CT volume and percent change of calcium hydroxide before and after irrigation were calculated and analyzed.Results: Calcium hydroxide paste remained in all groups after irrigation. The SI group removed -0.09% in the apical segment (0-3 mm) and 14% in the middle segment (3-6 mm). CUI removed 15% in the apical segment and 50% in the middle segment. LAI removed 49% in the apical segment and 72% in the middle segment.Conclusions: Irrigation with LAI removed significantly more calcium hydroxide in the middle and apical segments when compared to CUI and SI.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"1053-1060"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144191804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinician Perspective of Regenerative Endodontic Procedures for Immature Anterior Teeth: An Observational Web-based Study. 临床医生对未成熟前牙再生牙髓治疗的看法——一项基于网络的观察性研究。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-13 DOI: 10.1016/j.joen.2025.05.005

Alexander Brett, Federico Foschi, Shanon Patel

{"title":"Clinician Perspective of Regenerative Endodontic Procedures for Immature Anterior Teeth: An Observational Web-based Study.","authors":"Alexander Brett, Federico Foschi, Shanon Patel","doi":"10.1016/j.joen.2025.05.005","DOIUrl":"10.1016/j.joen.2025.05.005","url":null,"abstract":"Introduction: Regenerative endodontic procedures (REPs) are beneficial in preserving infected immature permanent teeth. The aim of this observational study was to assess treatment planning of REPs, geographical differences in their provision, clinical hesitancy and provision rate.Methods: Digitally manipulated radiographs showing varying degrees of root development in a maxillary central incisor with apical pathosis were used in an anonymized online questionnaire to assess clinical predisposition. The data were analyzed using descriptive statistics.Results: Among 160 participants (59.4% specialist endodontists), 73.1% favored REPs for the most immature apices, decreasing with root maturity. Specialist experience did not impact REP provision. UK endodontists performed REPs less frequently than international counterparts. Key perceived REP benefits included fracture resistance and root lengthening. Hesitancy stemmed from unfamiliarity (32.9%) and healing concerns (25.9%). Confidence varied and 25% had never performed a REP.Conclusion: REPs are generally appropriately treatment planned, although more education with regards to clinical suitability and case selection is suggested. Geographical variance in provision of REPs is apparent between the UK and non-UK endodontists. Hesitancy exists in the provision of REPs, and prevalence of provision is low.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"1061-1071"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Precooling the Anesthetic Agent in Comparison to Increasing the Dosage on the Success Rate of Inferior Alveolar Nerve Block Using Articaine in Mandibular First Molars with Symptomatic Irreversible Pulpitis: A Double-Blind, Randomized, Controlled Clinical Trial. 双盲、随机对照临床试验：麻醉药物预冷却与增加剂量对下颌第一磨牙下牙槽神经阻滞治疗症状不可逆性牙髓炎成功率的影响

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-28 DOI: 10.1016/j.joen.2025.05.011

Bafrin Fattahi, Negin Ghasemi, Sahar Shakouei, Mahsa Ladan Moghaddam

{"title":"Effect of Precooling the Anesthetic Agent in Comparison to Increasing the Dosage on the Success Rate of Inferior Alveolar Nerve Block Using Articaine in Mandibular First Molars with Symptomatic Irreversible Pulpitis: A Double-Blind, Randomized, Controlled Clinical Trial.","authors":"Bafrin Fattahi, Negin Ghasemi, Sahar Shakouei, Mahsa Ladan Moghaddam","doi":"10.1016/j.joen.2025.05.011","DOIUrl":"10.1016/j.joen.2025.05.011","url":null,"abstract":"Introduction: Achieving anesthesia in mandibular molar teeth with irreversible pulpitis has been challenging. This study aimed to compare the efficacy of precooling articaine and increasing its dosage for inferior alveolar nerve block (IANB) in the mandibular first molars with symptomatic irreversible pulpitis.Methods: In this randomized double-blind clinical trial, 90 first mandibular molar teeth with symptomatic irreversible pulpitis (visual analog scale >54) were randomly divided into three groups and received conventional IANB injection with 1.8 mL (1 cartridge) 1C group or 3.6 mL (two cartridges) 2C group of 4% articaine with 1:200,000 epinephrine with room temperature or 1.8 ml of the same agent with 4°C temperature cold cartridge (CC) group. The patients recorded their pain during the injection and access cavity preparation, pulp chamber, and root canal entrance using a visual analog scale. Data were analyzed by repeated measure of analysis of variance and Bonferroni tests (P < .05).Results: In all three groups, the pain levels at different injection times were significantly different (P < .05). The pain level did not differ significantly between the 2C group and the CC group (P > .05), but the difference was significant between the 1C group and 2C groups (P < .05) and also between the 1C group and CC group (P < .05).Conclusions: Administration of cold articaine provides a significantly higher success rate of IANBs in mandibular first molar teeth with symptomatic irreversible pulpitis compared to IANB with articaine kept at room temperature; however, increasing the dosage of articaine led to a significantly higher success rate.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"989-995"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144187067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of Type 2 Diabetes Mellitus on Apical Periodontitis Size, Endotoxin Levels, and Bacterial Load in Root Canals with Necrotic Pulp. 2型糖尿病对坏死牙髓根管内牙周炎大小、内毒素水平及细菌负荷的影响。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-09 DOI: 10.1016/j.joen.2025.05.003

Mirela Cesar Barros, Evelyn Giuliana Velasquez-Espedilla, Luciane Dias de Oliveira, Brenda Paula F A Gomes, Marco Antônio Hungaro Duarte, Carlos Antônio Negrato, Flaviana Bombarda Andrade

{"title":"Impact of Type 2 Diabetes Mellitus on Apical Periodontitis Size, Endotoxin Levels, and Bacterial Load in Root Canals with Necrotic Pulp.","authors":"Mirela Cesar Barros, Evelyn Giuliana Velasquez-Espedilla, Luciane Dias de Oliveira, Brenda Paula F A Gomes, Marco Antônio Hungaro Duarte, Carlos Antônio Negrato, Flaviana Bombarda Andrade","doi":"10.1016/j.joen.2025.05.003","DOIUrl":"10.1016/j.joen.2025.05.003","url":null,"abstract":"Introduction: This clinical study was conducted to describe the endodontic microbiological profile of teeth with apical periodontitis in patients with type 2 diabetes mellitus (T2DM), focusing on bacterial count and endotoxin levels found in primary infection, as well as the size of periapical lesions, determined by cone-beam computed tomography (CBCT) analysis.Methods: Thirty-four patients were selected and divided into 2 groups: 17 with T2DM and 17 nondiabetic controls. Anamnesis, clinical examinations, and radiographic evaluations were performed. Root canal samples were collected using sterile/pyrogen-free paper tips. Endotoxins were quantified using limulus amoebocyte lysate assay, and bacterial counts (colony forming units/mL) were determined using anaerobic culture techniques. The periapical status of the teeth was assessed using the CBCT periapical index. Data were analyzed using Pearson correlation, simple linear regression, t-tests, and Chi-square tests (P < .05).Results: A larger size of periapical lesions (CBCT periapical index ≥4) was observed in patients with T2DM (64.8%) compared to controls (17.7%) (P < .05). There was a significant increase in the number of bacteria by culture and endotoxin levels in diabetic patients. A positive correlation was found between elevated endotoxin levels and the diameter of periapical lesions (P < .05).Conclusions: This study supports the hypothesis that T2DM is associated with a greater microbial load and higher endotoxin levels, contributing to more severe periapical bone destruction, which may negatively affect endodontic treatment outcomes in these patients.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"1036-1043"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Presence of Epstein-Barr Virus in Periapical Pathoses: A Systematic Review and Meta-Analysis. 爱泼斯坦-巴尔病毒在根尖周围疾病中的存在——一项系统综述和荟萃分析。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-09 DOI: 10.1016/j.joen.2025.05.002

Jagveer Yadav, Mala Kamboj, Gopikrishnan Vijayakumar, Anjali Narwal, Anju Devi, Adarsh Kumar, R Keerthika

{"title":"Presence of Epstein-Barr Virus in Periapical Pathoses: A Systematic Review and Meta-Analysis.","authors":"Jagveer Yadav, Mala Kamboj, Gopikrishnan Vijayakumar, Anjali Narwal, Anju Devi, Adarsh Kumar, R Keerthika","doi":"10.1016/j.joen.2025.05.002","DOIUrl":"10.1016/j.joen.2025.05.002","url":null,"abstract":"Background: Periapical lesions, often arising from polymicrobial infections, exhibit varied clinical manifestations. The role of herpes viruses, particularly Epstein-Barr virus (EBV), in the etiopathogenesis of these conditions remains underexplored despite evidence that suggests its involvement in inflammatory processes.Objective: This systematic review and meta-analysis aimed to evaluate the presence of EBV in periapical diseases and to assess its probable role in their etiopathogenesis.Material and methods: Literature searches were conducted across PubMed, Scopus, Web of Science, and Google Scholar until July 2024. Original research that examined the association of EBV in periapical diseases was included for review. The risk of bias for each study was assessed using the modified Newcastle-Ottawa scale and Review Manager 5.4 was used to generate the results.Results: A total of 31 studies were included for qualitative synthesis, with 15 contributing to quantitative analysis. Findings revealed higher EBV detection rates in periapical lesions, particularly in symptomatic cases and lesions with larger sizes. Meta-analysis using random effects models also affirmed that EBV-positive transcripts significantly enhanced the risk of periapical lesions and symptomatic cases.Conclusion: This review highlights a significant association between EBV and periapical lesions, particularly in symptomatic cases and larger lesions. The findings warrant further investigation into the mechanisms by which EBV contributes to the pathogenesis of periapical diseases and the implications for clinical management, including potential antiviral therapies.Prospero registration number: CRD4202344796.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"1006-1020"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An In Vitro Tube Model for Cell Biocompatibility Study of Capping Materials for Regenerative Endodontics. 再生牙髓学盖层材料细胞生物相容性的体外试管模型研究。

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 Epub Date: 2025-05-09 DOI: 10.1016/j.joen.2025.04.018

Nela Pilbauerova, Divyamaanasa Dasi, Isaac J de Souza Araujo, George T-J Huang

{"title":"An In Vitro Tube Model for Cell Biocompatibility Study of Capping Materials for Regenerative Endodontics.","authors":"Nela Pilbauerova, Divyamaanasa Dasi, Isaac J de Souza Araujo, George T-J Huang","doi":"10.1016/j.joen.2025.04.018","DOIUrl":"10.1016/j.joen.2025.04.018","url":null,"abstract":"Introduction: Cell-based pulp regeneration utilizes capping materials underneath a final restoration to seal the tooth after delivering cells into the canal system. Studying the immediate biocompatibility of materials with injected cells has been challenging. This study aimed to utilize a tube model to observe cell response directly and conveniently to the capping materials in a cell culture in vitro system.Methods: A tapered plastic tube (14 mm in length, 2 mm diameter of the apex opening) to simulate root canal space was filled with fluorescence-labeled dental pulp cells mixed in fibrin gel and the larger end sealed with various capping materials including Mineral Trioxide Aggregate (MTA), Biodentine, hydroxyapatite-tricalcium phosphate (HA-TCP), composite, and glass ionomer. The tube was placed in wells of culture plates and incubated for various times in vitro and observed under the microscope for cell morphological changes. pH changes within the tube were monitored over time.Results: Both fresh MTA and Biodentine caused adverse response to the cells in the tube. Only a few normally growing cells were observed at the apical end. Composite or glass ionomer appeared better tolerated by cells. HA-TCP mixed in fibrin gel demonstrated the highest compatibility with cells; however, using HA-TCP to separate cells from fresh MTA or Biodentine did not reduce the negative effect of these 2 calcium silicate cements. The pH increased within the tube may explain the observed toxicity.Conclusions: Using HA-TCP mixed in fibrin gel as a capping material appears highly biocompatible to cells while fresh MTA and Biodentine are not.","PeriodicalId":15703,"journal":{"name":"Journal of endodontics","volume":" ","pages":"1072-1080"},"PeriodicalIF":3.6,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12335931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143996955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insights Into the Aug 2025 Issue of the JOE. 《JOE》2025年8月刊洞察

IF 3.6 2区医学

Journal of endodontics Pub Date : 2025-08-01 DOI: 10.1016/j.joen.2025.07.010

Anita Aminoshariae, Amir Azarpazhooh, Gerald N Glickman, Jianing He, Sahng G Kim, Anil Kishen, Ariadne M Letra, Linda Levin, Ronald Ordinola-Zapata, Frank C Setzer, Franklin R Tay, Kenneth M Hargreaves

引用次数: 0