Journal of Fermentation and Bioengineering最新文献_第5页

Ethanol-induced water stress and fungal growth 乙醇诱导水分胁迫和真菌生长

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80150-5

John E. Hallsworth, Yoshiyuki Nomura, Masayoshi Iwahara

{"title":"Ethanol-induced water stress and fungal growth","authors":"John E. Hallsworth, Yoshiyuki Nomura, Masayoshi Iwahara","doi":"10.1016/S0922-338X(98)80150-5","DOIUrl":"10.1016/S0922-338X(98)80150-5","url":null,"abstract":"<div>Fungal growth inhibition by ethanol was compared with that caused by five other agents of water stress (at 25, 40 and 42.5°C), using Aspergillus oryzae. Ethanol, KCl, glycerol, glucose, sorbitol, and polyethylene glycol 400 were incorporated into media at concentrations corresponding to water activity (aw) values in the range 1 to 0.75. Generally, as temperature increased there was a decrease in the aw value at which optimum growth occurred. The aw limit for growth on KCl, glycerol, glucose, sorbitol, or polyethylene glycol 400 media was about 0.85, regardless of temperature. However, the aw limit for growth on ethanol media varied between 0.97 and 0.99 aw and was temperature-dependent. Water stress accounted for up to 31, 18 and 6% of growth inhibition by ethanol at 25, 40, and 42.5°C, respectively. For media containing ethanol, the decrease in growth rate per unit of aw reduction was greater as temperature increased. However, ethanol-induced water stress remained constant regardless of temperature, suggesting that other inhibitory effects of ethanol are closely temperature-dependent. Water stress may account for considerably more than 30% of growth inhibition by ethanol in cells that remain metabolically active at higher ethanol concentrations.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 5","pages":"Pages 451-456"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80150-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90539413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 68

Purification and characterization of β-N-acetylhexosaminidase from Streptomyces sp. no. 499 链霉菌β- n -乙酰己糖氨酸酶的纯化及特性研究。499

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80110-4

引用次数: 0

Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots 考虑毛状根高密度培养过程中生物量变化的生物量估算程序

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80095-0

Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu

{"title":"Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots","authors":"Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu","doi":"10.1016/S0922-338X(98)80095-0","DOIUrl":"10.1016/S0922-338X(98)80095-0","url":null,"abstract":"<div>The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of Catharanthus roseus hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./l, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./l) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./l of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 454-457"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80095-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91340823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Synthesis of the four possible stereoisomers of 21-methyl-8-pentatriacontene, the female contact sex pheromone of the yellow-spotted longicorn beetle, Psacothea hilaris 黄斑天牛雌性接触性信息素21-甲基-8-五三康内烯四种可能立体异构体的合成

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)80366-2

Eiichiro Fukusaki , Shiro Satoda , Hiroyuki Yuasa , Shuji Senda , Tetsuo Omata , Midori Fukaya , Sadao Wakamura

引用次数: 6

The importance of ionic strength as a parameter in screening peptide ligands from a phage display library 离子强度作为筛选噬菌体展示文库中肽配体的参数的重要性

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80093-7

Yoshio Katakura, Eun Tae Lim, Setsuo Tsujii, Takeshi Omasa, Ken-Ichi Suga

引用次数: 2

Production of erythritol in fed-batch cultures of Trichosporon sp. Trichosporon sp.分批培养中赤藓糖醇的生产。

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)80010-5

Jinbyung Park , Byungcheol Seo , Jungryul Kim , Yongkun Park

引用次数: 51

Biological activities of abscisic acid analogs in the morphological change of the green alga Haematococcus pluvialis 脱落酸类似物在绿藻雨红球藻形态变化中的生物活性

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80076-7

Makio Kobayashi , Yasushi Todoroki , Nobuhiro Hirai , Yoshiro Kurimura , Hajime Ohigashi , Yasunobu Tsuji

引用次数: 16

Cloning and sequencing of the gene encoding chitinase ChiA from Xanthomonas sp. strain AK and some properties of ChiA 黄单胞菌AK几丁质酶ChiA基因的克隆、序列分析及ChiA的一些特性

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)80001-4

Kazuo Sakka , Ryo Kusaka , Akihiro Kawano , Shuichi Karita , Jiraporn Sukhumavasi , Tetsuya Kimura , Kunio Ohmiya

引用次数: 19

Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis 搅拌陶瓷膜反应器过滤性能的改进及其在乳酸乳球菌密集细胞培养快速发酵乳酸中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80087-1

Yuya Kamoshita, Ryo Ohashi, Takahiro Suzuki

{"title":"Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis","authors":"Yuya Kamoshita, Ryo Ohashi, Takahiro Suzuki","doi":"10.1016/S0922-338X(98)80087-1","DOIUrl":"10.1016/S0922-338X(98)80087-1","url":null,"abstract":"<div>A ceramic filter was fitted in a stirred ceramic membrane reactor (SCMR) for both extraction of culture supernatant and feeding of distilled water in reverse flow. The dependence of filtration performance on the cell concentration was decreased by about 20% by regularly cleaning the filter using a membrane cleaning system. The improved permeability effected an increase of both the growth rate and viability of Lactococcus lactis by increasing the dilution rate of the culture supernatant. Using the improved SCMR system, a cell concentration of 178 g/l and viability of 98% were obtained after 198 h of culture, while it took 238 h to obtain a cell concentration of 141 g/l and 94% viability without the use of the membrane cleaning system. The perfusion culture system was applied to the rapid batch fermentation of lactic acid by retaining cells at a high density in the SCMR. When the cell concentration reached 80 g/l, the culture supernatant was extracted and replaced with the fermentation medium. Batch fermentation using the retained cells was repeated six times. The concentration of lactic acid increased to more than 30 g/l within 2 h in each fermentation, while 1.2 h was necessary for replacing the culture supernatant to repeat the batch fermentation. The production rate of lactic acid was increased in proportion to the cell concentration, and a high fermentation activity of the retained cells was maintained via the repeated batch fermentation. These results demonstrate that the improved permeability of the SCMR with use of a membrane cleaning system effected a rapid increase in the concentration and viability of cells, and accordingly, the increased production rate of lactic acid in proportion to the concentration of viable cells.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 422-427"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80087-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75956811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Chitinolytic enzyme activity of Penicillium janthinellum P9 in bench-top bioreactor 台式生物反应器中紫青霉P9几丁质水解酶活性的研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)80020-8

Massimiliano Fenice , Jean-Louis Leuba , Federico Federici

引用次数: 42