Journal of Fermentation and Bioengineering最新文献

{"title":"Preparation of surfactant-coated lipases utilizing the molecular imprinting technique","authors":"Noriho Kamiya ,&nbsp;Masahiro Goto","doi":"10.1016/S0922-338X(97)86774-8","DOIUrl":"10.1016/S0922-338X(97)86774-8","url":null,"abstract":"<div><p>Modulation of the enantioselectivity of two lipases exhibiting different specificities in the esterification of 2-octanol with lauric acid was investigated by utilizing surfactant-coating and molecular imprinting techniques. The enantioselectivity of the surfactant-coated lipase from <em>Pseudomonas cepacia</em> (PS) was enhanced in the presence of (<em>R</em>)-2-octanol as a print molecule, whereas the enantioselectivity of the lipase from <em>Candida cylindracea</em> (AY) was hardly changed by the imprinting technique. In the case of lipase AY, however, the selectivity of the native enzyme toward the (<em>R</em>)-isomer was changed to a preference for the (<em>S</em>)-isomer as a result of being coating with surfactant molecules. The molecular imprinting effect was prominent in the case of lipase PS, its enantioselectivity in the formation of 2-octanoate in isooctane being enhanced around two-fold compared with that of the native enzyme.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 2","pages":"Pages 237-239"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)86774-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86992390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modelling of ferrous sulphate oxidation by Thiobacillus ferrooxidans in discontinuous culture: Influence of temperature, pH and agitation rate","authors":"JoséManuel Gómez,&nbsp;Domingo Cantero","doi":"10.1016/S0922-338X(98)80038-X","DOIUrl":"10.1016/S0922-338X(98)80038-X","url":null,"abstract":"<div><p>This paper studies the temperature effect on the specific growth rate of the microorganisms (<em>μ</em>_c) as particular aspect of the mathematical relationships between <em>μ</em>_c of <em>Thiobacillus ferrooxidans</em> and the concentrations of substrate (ferrous iron) and product (ferric iron). Until now, there have been few studies of this relationship; also, there has been little work studying the influence of temperature on growth kinetics of this bacteria. In this paper, an extensive experimental phase has been developed, with the aim of determining with precision the influence of temperature on the maximum specific growth rate (<em>μ</em>_max). The influence on the growth rate of <em>T. ferrooxidans</em> exerted by the initial pH of the medium and by agitation rate has also been studied.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 79-83"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80038-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82459034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Key word index","authors":"","doi":"10.1016/S0922-338X(97)85694-2","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)85694-2","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 3","pages":"Page ii"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)85694-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137198327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation of kefiran-producing lactic acid bacteria from kefir grain and improvement of kefiran productivity","authors":"","doi":"10.1016/S0922-338X(99)80022-1","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)80022-1","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 6","pages":"Page 624"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)80022-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136499621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell concentration dependence of dynamic viscoelasticity of Escherichia coli culture suspensions","authors":"Kiyoshi Toda ,&nbsp;Hisamoto Furuse ,&nbsp;Takeshi Amari ,&nbsp;Xianfu Wei","doi":"10.1016/S0922-338X(98)80085-8","DOIUrl":"10.1016/S0922-338X(98)80085-8","url":null,"abstract":"<div><p>The viscoelastic properties of <em>Escherichia coli</em> culture suspensions of various cell concentrations were studied in terms of relation to shear strain amplitude and angular frequency dependence. Samples of the suspensions were prepared by resuspension of a concentrated suspension obtained by centrifugation. Dynamic viscoelasticity for all cell concentrations at an angular frequency of <em>ω</em>=0.1 rad·s⁻¹ exhibited linear behavior up to a shear strain amplitude of about 0.02. The storage modulus decreased with an increase in shear strain amplitude above 0.02, while dynamic viscosity was not so noticeably influenced. Further, for samples of very high cell concentrations in particular, the dynamic viscosity increased with increasing shear strain amplitude, in parallel with the decrease in storage modulus. The angular frequency dependence of the storage modulus for samples of high cell concentrations was very low, since the network of the suspension behaved as a relaxation mechanism having a long relaxation time. Marked dependence of viscoelasticity on cell concentration was observed, and considered to be caused by the development of a network structure composed of microbial floccules interacting wigh one another.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 410-415"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80085-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81701952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cloning, nucleotide sequence, and transcriptional analysis of Aspergillus aculeatus No. F-50 cellobiohydrolase I (cbhI) gene","authors":"G. Takada, T. Kawaguchi, J. Sumitani, M. Arai","doi":"10.1016/S0922-338X(97)80345-5","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80345-5","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"52 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74755783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequence Analysis of the Genes Encoding a Multicomponent Dioxygenase Involved in Oxidation of Aniline and o-Toluidine in Acinetobacter sp. Strain YAA","authors":"M. Takeo, Toshiki Fujii, Y. Maeda","doi":"10.1016/S0922-338X(97)80347-9","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80347-9","url":null,"abstract":"","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"47 4 1","pages":"17-24"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77106113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-free esterification of oleic acid and oleyl alcohol using membrane reactor and lipase-surfactant complex","authors":"Yasuyuki Isono ,&nbsp;Mitsutoshi Nakajima ,&nbsp;Hiroshi Nabetani","doi":"10.1016/S0922-338X(98)80048-2","DOIUrl":"10.1016/S0922-338X(98)80048-2","url":null,"abstract":"<div><p>Oleyl alcohol and oleic acid were esterified using lipase-surfactant complex (LSC) in a solvent-free reaction system. LSC showed a higher reaction rate than the crude enzyme in the reaction system. Optimum LSC activity was obtained at 180 mg/g-solution water addition. A preliminary study of the membrane reactor system using a microfiltration membrane and a solvent-free reaction was performed, and the feasibility of the reactor system was confirmed.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 138-140"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80048-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88915541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anthocyanin synthesis, growth and nutrient uptake in suspension cultures of strawberry cells","authors":"Wei Zhang ,&nbsp;Minoru Seki ,&nbsp;Shintaro Furusaki ,&nbsp;Anton P.J. Middelberg","doi":"10.1016/S0922-338X(98)80037-8","DOIUrl":"10.1016/S0922-338X(98)80037-8","url":null,"abstract":"<div><p>Strawberry (<em>Fragaria ananassa</em> cv. Shikinari) cell suspension cultures carried out in shake flasks for 18 d were closely examined for cell growth, anthocyanin synthesis and the development of pigmented cells in relation to the uptake of carbohydrate, extracellular PO₄, NO₃, NH₄, and calcium. Cell viability, extracellular anthocyanin content, pH and electrical conductivity of the broth were also monitored. The specific growth rate of strawberry cells at exponential phase was 0.27 and 0.28 d⁻¹ based on fresh and dry weight, respectively. Anthocyanin synthesis was observed to increase continuously to a maximum value of 0.86 mg/g fresh cell weight (FCW) at day 6, and was partially growth-associated. Anthocyanin synthesis was linearly related to the increase in pigmented cell ratio, which increased with time and reached a maximum value of ca. 70% at day 6 due to reduction in cell viability and depletion of substrate. Total carbohydrate uptake was closely associated with increase in cell growth, and glucose was utilized in preference to fructose. Nitrate and ammonia were consumed until 9 d of culture, but phosphate was completely absorbed within 4 d. Calcium was assimilated throughout the growth cycle. After 9 d, cell lysis was observed which resulted in the leakage of intracellular substances and a concomitant pH rise. Anthocyanin was never detected in the broth although the broth became darkly pigmented during the lysis period. This suggests that anthocyanin was synthesized only by viable pigmented cells, and degraded rapidly upon cell death and lysis. Based on the results of kinetic analysis, a model was developed by incorporating governing equations for the ratio of pigmented cells into a Bailey and Nicholson's model. This was verified by comparison with the experimental data. The results suggest that the model satisfactorily describes the strawberry cell culture process, and may thus be used for process optimization.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 72-78"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80037-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78794295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Riboflavin biosynthetic genes of Corynebacterium ammoniagenes","authors":"Satoshi Koizumi ,&nbsp;Sadao Teshiba","doi":"10.1016/S0922-338X(98)80046-9","DOIUrl":"10.1016/S0922-338X(98)80046-9","url":null,"abstract":"<div><p>Riboflavin-requiring mutants were isolated from <em>Corynebacterium ammoniagenes</em>. One of the mutants, RK122, accumulated 6,7-dimethyl-8-ribityllumazine (DMRL), a direct precursor of riboflavin, in the culture medium. Chromosomal DNA fragments that complement the riboflavin requirement of RK122 were cloned and sequenced. Sequence analysis revealed that the structure of the riboflavin biosynthetic genes of <em>C. ammoniagenes</em> showed significant similarity to those of <em>Bacillus subtilis</em>.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 130-133"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80046-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73934734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}