Journal of Fermentation and Bioengineering最新文献_第9页

Expression and use of Methanobacterium thermoautotrophicum sn-glycerol 1-phosphate dehydrogenase for the assay of sn-glycerol 1-phosphate in Archaea 热自养甲烷杆菌sn-甘油1-磷酸脱氢酶在古细菌中表达及应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80128-1

Shunsuke Noguchi , Makoto Maeda , Masateru Nishihara , Yousuke Koga , Nobuhito Sone

{"title":"Expression and use of Methanobacterium thermoautotrophicum sn-glycerol 1-phosphate dehydrogenase for the assay of sn-glycerol 1-phosphate in Archaea","authors":"Shunsuke Noguchi , Makoto Maeda , Masateru Nishihara , Yousuke Koga , Nobuhito Sone","doi":"10.1016/S0922-338X(98)80128-1","DOIUrl":"10.1016/S0922-338X(98)80128-1","url":null,"abstract":"<div>sn-glycerol-1-phosphate (G-1-P) dehydrogenase is the key enzyme for biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of Archaea. The gene encoding this enzyme in Methanobacterium thermoautotrophicum (egsA) was used to construct an expression plasmid pTrcG1Pdh for Escherichia coli. The G-1-P dehydrogenase activity of E. coli XL1-blue/pTrcG1Pdh was maximal 8–10 h after induction. The expressed G-1-P dehydrogenase was purified 4300-fold from the soluble fraction to homogeniety after 4300 times purification by a procedure consisting of fractionation with ammonium sulfate precipitation, and hydrophobic and ion-exchange chromatographies. The yield was about 70%. The Vmax value for the forward reaction to produce G-1-P was 740 units (μmol/min)/mg, with a Km of 0.21 mM for NADH and 0.39 mM for dihydroxyacetone phosphate. The Km's for G-1-P and NAD+ in the backward reaction were 10.5 and 0.46 mM, respectively. These kinetic constants are similar to those for the enzyme from M. thermoautotrophicum. G-1-P dehydrogenase was successfully used to analyze the stereospecificity of glycerophosphate, which is an intermediate of phospholipid biosynthesis and glycerol metabolism; the rate of NADH formation was proportional to the G-1-P concentration up to 3 mM in the presence of 0.02 unit of the purified enzyme.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 3","pages":"Pages 266-270"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80128-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78501046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from soil polluted with this contaminant 污染土壤中新分离的三氯乙烯降解菌的系统发育分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)80003-8

S. Hanada, T. Shigematsu, Katsutoshi Shibuya, M. Eguchi, Takeshi Hasegawa, F. Suda, Y. Kamagata, T. Kanagawa, R. Kurane

引用次数: 40

Production of d-talitol from d-psicose by Candida famata R28 famata假丝酵母R28从d-psicose生产d-talitol

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80359-5

Hiroyuki Sasahara , Masaki Mine , Ken Izumori

引用次数: 28

Nitrogen removal and N2O emission in a full-scale domestic wastewater treatment plant with intermittent aeration 间歇式曝气生活污水处理厂氮去除及N2O排放研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80114-1

Yuzuru Kimochi , Yuhei Inamori , Motoyuki Mizuochi , Kai-Qin Xu , Masatoshi Matsumura

{"title":"Nitrogen removal and N2O emission in a full-scale domestic wastewater treatment plant with intermittent aeration","authors":"Yuzuru Kimochi , Yuhei Inamori , Motoyuki Mizuochi , Kai-Qin Xu , Masatoshi Matsumura","doi":"10.1016/S0922-338X(98)80114-1","DOIUrl":"10.1016/S0922-338X(98)80114-1","url":null,"abstract":"<div>Nitrous oxide (N2O) is emitted from wastewater treatment processes. It is known as a greenhouse gas that contributes to global warming (over 200 times more per molecule than carbon dioxide) and to the destruction of the ozone layer. It is therefore of great importance to develop technology that can suppress N2O emission. The effects of an anoxic period on N2O emission and nitrogen removal were investigated in an actual domestic wastewater treatment plant. When operated with intermittent aeration, most of the N2O was emitted into the atmosphere during the aerobic period. N2O emission from the intermittent process was estimated to be 0.43–1.89 g N2O person−1 year−1. Maintaining a dissolved oxygen (DO) concentration of over 0.5 mg l−1 during the aerobic period resulted in the complete conversion of the influent NH4-N to NO3-N and a 60-min anoxic period was sufficient for denitrification to be completed. The findings show that an optimum combination of aerobic and anoxic conditions and their suitable control are very important for improving nitrogen removal efficiency and controlling N2O emission.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 2","pages":"Pages 202-206"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80114-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85162979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 185

Purification and comparison of phosphoglycerate kinases from nitrifying bacteria 硝化细菌中磷酸甘油酸激酶的纯化与比较

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)89002-3

Yasunori Mizuno, Mifuyu Ohshima, Yasue Yao, Rie Shibasaki, Reiji Takahashi, Tatsuaki Tokuyama

{"title":"Purification and comparison of phosphoglycerate kinases from nitrifying bacteria","authors":"Yasunori Mizuno, Mifuyu Ohshima, Yasue Yao, Rie Shibasaki, Reiji Takahashi, Tatsuaki Tokuyama","doi":"10.1016/S0922-338X(99)89002-3","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)89002-3","url":null,"abstract":"<div>Phosphoglycerate kinases [PGK, EC 2.7.2.3] were purified as electrophoretically homogeneous proteins from four nitrifying bacteria: Nitrosomonas europaea ATCC 25978T (Ns), Nitrosomonas sp. TNO632 (TNO), Nitrosomonas sp. K1 (K1), Nitrobacter winogradskyi IFO 14297 (Nb) and nonsulfur bacterium Rhodopseudomonas palustris JCM2524 (Rp). The enzymes were all monomers with molecular masses of 44.6, 44.3, 43.7, 46.1, and 43.4 kDa, respectively. Ns-PGK and Nb-PGK had the same pH-activity curves with an optimum pH range of 8.0–8.5. The enzymes were stable in the pH range 7.0–9.0 when kept at 4°C for 48 h. The temperature optima of Ns-PGK and Nb-PGK were 50 and 40°C, respectively. Both enzymes were strongly inhibited by pCMB and SDS at 1 mM, ATP was effective, while other nucleotides did not serve as a phosphate donor. The N-terminal amino acid sequences of Ns-PGK and Nb-PGK were maximally homologous (90–95%) with TNO-PGK and Rp-PGK, respectively. However, the degree of PGK homology was as low as 45–59% between the two nitrifying bacteria genera. As previously observed, all Nitrosomonas and Nitrobacter strains constitute a monophyletic assemblage within the beta and alpha subdivisions of the proteobacteria, respectively. The differences in the N-terminal amino acid sequences of the PGKs coincided with the taxonomic differences of the bacterial genera according to the molecular phylogenetic tree.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 4","pages":"Pages 346-350"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89002-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91610912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from soil polluted with this contaminant 污染土壤中新分离的三氯乙烯降解菌的系统发育分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)80003-8

Satoshi Hanada , Toru Shigematsu , Katsutoshi Shibuya , Masahiro Eguchi , Takeshi Hasegawa , Fusako Suda , Yoichi Kamagata , Takahiro Kanagawa , Ryuichiro Kurane

{"title":"Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from soil polluted with this contaminant","authors":"Satoshi Hanada , Toru Shigematsu , Katsutoshi Shibuya , Masahiro Eguchi , Takeshi Hasegawa , Fusako Suda , Yoichi Kamagata , Takahiro Kanagawa , Ryuichiro Kurane","doi":"10.1016/S0922-338X(99)80003-8","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)80003-8","url":null,"abstract":"<div>Five methanotrophs (strains 18-2, EB1, KSWIII, KSPIII and KSPIII) and three aromatic compound oxidizers (strains KP22, KP24 and KT1) were isolated from the natural field polluted with trichloroethylene (TCE). Phylogenetic analysis based on 16S rRNA gene sequence suggested that all of the isolates belonged to the class Proteobacteria. Two of the methanotrophic isolates, strains 18-2 and EB1, were closely related to Methylocystis sp. strain M in the α subclass of Proteobacteria with sequence similarities of 98.2–98.4%, while strains KSWIII, KSPIII and KSPII were akin to Methylomonas methanica in the γ subclass of Proteobacteria with sequence similarities of 97.8–98.1%. The aromatic compounds oxidizers, strains KP22, KP24 and KT1, were assigned to the β subclass of Proteobacteria, and classified as Bordetella sp. (97.2–97.8% sequence similarity to species of the genus Bordetella), Burkholderia cepacia (99.2%) and Ralstonia eutropha (99.4%), respectively. All isolates degraded TCE when cells were grown with the appropriate substrate, i.e., methane, phenol or toluene. Detailed kinetic analyses of their TCE degradation revealed that the rates of degradation (k1) among the isolates were 10–36 ml of TCE/mg of dry cell weight/h, and the transformation capacities (Tc) were 0.01–0.13 mg of TCE/mg of dry cell weight.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 6","pages":"Pages 539-544"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)80003-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91647852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38

Discovery of a new mechanism of 2,3-butanediol stereoisomer formation in Bacillus cereus YUF-4 蜡样芽孢杆菌YUF-4中2,3-丁二醇立体异构体形成新机制的发现

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80358-3

Sadaharu Ui, Takeshi Hosaka, Kazuhide Watanabe, Akio Mimura

{"title":"Discovery of a new mechanism of 2,3-butanediol stereoisomer formation in Bacillus cereus YUF-4","authors":"Sadaharu Ui, Takeshi Hosaka, Kazuhide Watanabe, Akio Mimura","doi":"10.1016/S0922-338X(97)80358-3","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80358-3","url":null,"abstract":"<div>A mechanism of 2,3-butanediol (BD) stereoisomer formation was examined with respect to the BD cycle. The enzymes acetylacetoin synthase, acetylacetoin reductase (AACR), and acetylbutanediol hydrolase (ABDH), which are part of the BD cycle, were found to be present in the cell-free extract of the bacterial strain Bacillus cereus YUF-4. Two kinds of acetylbutanediol (ABD) stereoisomers were produced in the reduction of acetylacetoin (AAC) by AACR, which were identified as having 3R,4R and 3S,4R configurations by NMR spectroscopy and an enzymic method. The two ABD formations were found to be catalyzed independently by two respective enzymes: the former was catalyzed by a NADPH-dependent AACR (3R,4R-ABD forming) and the latter by a NADH-dependent AACR (3S,4R-ABD forming). The 3R,4R-ABD was converted into R,R-BD and the 3S,4R-ABD into R,S-BD by intracellular ABDH. These findings demonstrated the existence of a new BD isomer formation mechanism derived from the BD cycle.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 1","pages":"Pages 79-83"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)80358-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91704875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Application of the random amplified polymorphic DNA (RAPD) technique to distinguishing species of the red tide phytoplankton Chattonella (Raphydophyceae) 随机扩增多态性DNA (RAPD)技术在红潮浮游植物查通菌种类鉴定中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)85687-5

Eiko Murayama-Kayano , Sadaaki Yoshimatsu , Toshiaki Kayano , Takeshi Nishio , Hiroshi Ueda , Teruyuki Nagamune

引用次数: 8

Key word index 关键词索引

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)90013-7

引用次数: 0

Effect of ethanol on bacterial cellulose production from fructose in continuous culture 连续培养中乙醇对细菌从果糖中生产纤维素的影响

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80012-3

Takaaki Naritomi, Tohru Kouda, Hisato Yano, Fumihiro Yoshinaga

{"title":"Effect of ethanol on bacterial cellulose production from fructose in continuous culture","authors":"Takaaki Naritomi, Tohru Kouda, Hisato Yano, Fumihiro Yoshinaga","doi":"10.1016/S0922-338X(98)80012-3","DOIUrl":"10.1016/S0922-338X(98)80012-3","url":null,"abstract":"<div>Effect of ethanol on bacterial cellulose (BC) production from fructose by Acetobacter xylinum subsp. sucrofermentans BPR3001A was investigated in continuous culture. Supplementation of 10 g·l−1 ethanol to the feed medium containing 30 g·l−1 fructose as the main carbon source enhanced the ATP content of viable cells, cell concentration, and fructose consumption rate, establishing a 72-h steady state with 0.95 g·l−1·h−1 of BC production rate and 46% of BC yield in continuous culture with a dilution rate of 0.07 h−1. However, an increase in concentration of ethanol to more than 15 g·l−1 decreased BC production rate due to the inhibition of cell growth by the generated acetate. Results from batch culture experiment using ethanol as the main carbon source suggested that ethanol functioned as an energy source for ATP generation, and not as a substrate for BC biosynthesis. On the other hand, fructose hexokinase (FHK) activity was increased and activities of glucose 6-phosphate dehydrogenases (G6PDs) were inhibited by ATP, suggesting that the improved BC production from fructose by ethanol supplementation was a result of increased fructose dissimilation and abundant flow of glucose 6-phosphate (G6P), a precursor of BC, into the BC biosynthetic pathway due to the inhibition of G6PDs by increased levels of ATP.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 6","pages":"Pages 598-603"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80012-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90612240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 91