Journal of Fermentation and Bioengineering最新文献_第8页

Purification and comparison of phosphoglycerate kinases from nitrifying bacteria 硝化细菌中磷酸甘油酸激酶的纯化与比较

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)89002-3

Yasunori Mizuno, Mifuyu Ohshima, Yasue Yao, Rie Shibasaki, Reiji Takahashi, Tatsuaki Tokuyama

{"title":"Purification and comparison of phosphoglycerate kinases from nitrifying bacteria","authors":"Yasunori Mizuno, Mifuyu Ohshima, Yasue Yao, Rie Shibasaki, Reiji Takahashi, Tatsuaki Tokuyama","doi":"10.1016/S0922-338X(99)89002-3","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)89002-3","url":null,"abstract":"<div>Phosphoglycerate kinases [PGK, EC 2.7.2.3] were purified as electrophoretically homogeneous proteins from four nitrifying bacteria: Nitrosomonas europaea ATCC 25978T (Ns), Nitrosomonas sp. TNO632 (TNO), Nitrosomonas sp. K1 (K1), Nitrobacter winogradskyi IFO 14297 (Nb) and nonsulfur bacterium Rhodopseudomonas palustris JCM2524 (Rp). The enzymes were all monomers with molecular masses of 44.6, 44.3, 43.7, 46.1, and 43.4 kDa, respectively. Ns-PGK and Nb-PGK had the same pH-activity curves with an optimum pH range of 8.0–8.5. The enzymes were stable in the pH range 7.0–9.0 when kept at 4°C for 48 h. The temperature optima of Ns-PGK and Nb-PGK were 50 and 40°C, respectively. Both enzymes were strongly inhibited by pCMB and SDS at 1 mM, ATP was effective, while other nucleotides did not serve as a phosphate donor. The N-terminal amino acid sequences of Ns-PGK and Nb-PGK were maximally homologous (90–95%) with TNO-PGK and Rp-PGK, respectively. However, the degree of PGK homology was as low as 45–59% between the two nitrifying bacteria genera. As previously observed, all Nitrosomonas and Nitrobacter strains constitute a monophyletic assemblage within the beta and alpha subdivisions of the proteobacteria, respectively. The differences in the N-terminal amino acid sequences of the PGKs coincided with the taxonomic differences of the bacterial genera according to the molecular phylogenetic tree.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 4","pages":"Pages 346-350"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89002-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91610912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Expression and use of Methanobacterium thermoautotrophicum sn-glycerol 1-phosphate dehydrogenase for the assay of sn-glycerol 1-phosphate in Archaea 热自养甲烷杆菌sn-甘油1-磷酸脱氢酶在古细菌中表达及应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80128-1

Shunsuke Noguchi , Makoto Maeda , Masateru Nishihara , Yousuke Koga , Nobuhito Sone

{"title":"Expression and use of Methanobacterium thermoautotrophicum sn-glycerol 1-phosphate dehydrogenase for the assay of sn-glycerol 1-phosphate in Archaea","authors":"Shunsuke Noguchi , Makoto Maeda , Masateru Nishihara , Yousuke Koga , Nobuhito Sone","doi":"10.1016/S0922-338X(98)80128-1","DOIUrl":"10.1016/S0922-338X(98)80128-1","url":null,"abstract":"<div>sn-glycerol-1-phosphate (G-1-P) dehydrogenase is the key enzyme for biosynthesis of the enantiomeric glycerophosphate backbone of ether phospholipids of Archaea. The gene encoding this enzyme in Methanobacterium thermoautotrophicum (egsA) was used to construct an expression plasmid pTrcG1Pdh for Escherichia coli. The G-1-P dehydrogenase activity of E. coli XL1-blue/pTrcG1Pdh was maximal 8–10 h after induction. The expressed G-1-P dehydrogenase was purified 4300-fold from the soluble fraction to homogeniety after 4300 times purification by a procedure consisting of fractionation with ammonium sulfate precipitation, and hydrophobic and ion-exchange chromatographies. The yield was about 70%. The Vmax value for the forward reaction to produce G-1-P was 740 units (μmol/min)/mg, with a Km of 0.21 mM for NADH and 0.39 mM for dihydroxyacetone phosphate. The Km's for G-1-P and NAD+ in the backward reaction were 10.5 and 0.46 mM, respectively. These kinetic constants are similar to those for the enzyme from M. thermoautotrophicum. G-1-P dehydrogenase was successfully used to analyze the stereospecificity of glycerophosphate, which is an intermediate of phospholipid biosynthesis and glycerol metabolism; the rate of NADH formation was proportional to the G-1-P concentration up to 3 mM in the presence of 0.02 unit of the purified enzyme.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 3","pages":"Pages 266-270"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80128-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78501046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Fatty acid desaturation in methylotrophic yeast Hansenula polymorpha strain CBS 1976 and unsaturated fatty acid auxotrophic mutants 甲基营养酵母菌CBS 1976与不饱和脂肪酸营养缺陷突变体的脂肪酸去饱和

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80065-2

Sarintip Anamnart , Ilya Tolstorukov , Yoshinobu Kaneko , Satoshi Harashima

{"title":"Fatty acid desaturation in methylotrophic yeast Hansenula polymorpha strain CBS 1976 and unsaturated fatty acid auxotrophic mutants","authors":"Sarintip Anamnart , Ilya Tolstorukov , Yoshinobu Kaneko , Satoshi Harashima","doi":"10.1016/S0922-338X(98)80065-2","DOIUrl":"10.1016/S0922-338X(98)80065-2","url":null,"abstract":"<div>Analysis of fatty acid composition in wild-type cells of Hansenula polymorpha strain CBS 1976 revealed the presence of 18 : 1 (Δ9), 18 : 2 (Δ9,12) and 18 : 3 (Δ9,12,15) unsaturated fatty acids (UFAs), indicating that the α-linolenic desaturation pathway operates in this yeast. H. polymorpha cells also showed ability for uptake and incorporation of exogenous UFAs. By ethyl methanesulfonate mutagenesis, nine unsaturated fatty acid auxotrophic mutants of H. polymorpha were isolated. These mutants exhibited the growth arrest phenotype on nutrient medium and on nutrient medium supplemented with saturated fatty acids, but grew on media supplemented with various UFAs. Genetic analysis revealed that single recessive nuclear mutation conferred Ufa auxotrophy on these mutants. Fatty acid analysis by gas chromatography showed the accumulation of 18 : 0 but a decrease in the amount of 18 : 1 and 18 : 2 in mutant cells compared with the wild-type cells. Integrated physiological and genetical data suggested that mutations in all mutants occurred in one gene and probably led to defects in Δ9-desaturation pathway.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 5","pages":"Pages 476-482"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80065-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78874170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

An approach to high-level production of cephalosporin acylase from Pseudomonas strain N176 in Escherichia coli 假单胞菌N176在大肠杆菌中高效生产头孢菌素酰化酶的途径

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80019-6

Yuji Noguchi, Hisashi Yamada, Michitaka Yamaguchi, Katsuyuki Watanabe, Takao Fujimura, Yoshinori Ishii, Yoshimasa Saito

引用次数: 1

Key word index 关键词索引

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2003-10-21 DOI: 10.1016/S0922-338X(98)90013-7

引用次数: 0

Nitrogen removal and N2O emission in a full-scale domestic wastewater treatment plant with intermittent aeration 间歇式曝气生活污水处理厂氮去除及N2O排放研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80114-1

Yuzuru Kimochi , Yuhei Inamori , Motoyuki Mizuochi , Kai-Qin Xu , Masatoshi Matsumura

{"title":"Nitrogen removal and N2O emission in a full-scale domestic wastewater treatment plant with intermittent aeration","authors":"Yuzuru Kimochi , Yuhei Inamori , Motoyuki Mizuochi , Kai-Qin Xu , Masatoshi Matsumura","doi":"10.1016/S0922-338X(98)80114-1","DOIUrl":"10.1016/S0922-338X(98)80114-1","url":null,"abstract":"<div>Nitrous oxide (N2O) is emitted from wastewater treatment processes. It is known as a greenhouse gas that contributes to global warming (over 200 times more per molecule than carbon dioxide) and to the destruction of the ozone layer. It is therefore of great importance to develop technology that can suppress N2O emission. The effects of an anoxic period on N2O emission and nitrogen removal were investigated in an actual domestic wastewater treatment plant. When operated with intermittent aeration, most of the N2O was emitted into the atmosphere during the aerobic period. N2O emission from the intermittent process was estimated to be 0.43–1.89 g N2O person−1 year−1. Maintaining a dissolved oxygen (DO) concentration of over 0.5 mg l−1 during the aerobic period resulted in the complete conversion of the influent NH4-N to NO3-N and a 60-min anoxic period was sufficient for denitrification to be completed. The findings show that an optimum combination of aerobic and anoxic conditions and their suitable control are very important for improving nitrogen removal efficiency and controlling N2O emission.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 2","pages":"Pages 202-206"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80114-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85162979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 185

Application of the random amplified polymorphic DNA (RAPD) technique to distinguishing species of the red tide phytoplankton Chattonella (Raphydophyceae) 随机扩增多态性DNA (RAPD)技术在红潮浮游植物查通菌种类鉴定中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)85687-5

Eiko Murayama-Kayano , Sadaaki Yoshimatsu , Toshiaki Kayano , Takeshi Nishio , Hiroshi Ueda , Teruyuki Nagamune

引用次数: 8

Production of d-talitol from d-psicose by Candida famata R28 famata假丝酵母R28从d-psicose生产d-talitol

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)80359-5

Hiroyuki Sasahara , Masaki Mine , Ken Izumori

引用次数: 28

Discovery of a new mechanism of 2,3-butanediol stereoisomer formation in Bacillus cereus YUF-4 蜡样芽孢杆菌YUF-4中2,3-丁二醇立体异构体形成新机制的发现

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)80358-3

Sadaharu Ui, Takeshi Hosaka, Kazuhide Watanabe, Akio Mimura

{"title":"Discovery of a new mechanism of 2,3-butanediol stereoisomer formation in Bacillus cereus YUF-4","authors":"Sadaharu Ui, Takeshi Hosaka, Kazuhide Watanabe, Akio Mimura","doi":"10.1016/S0922-338X(97)80358-3","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80358-3","url":null,"abstract":"<div>A mechanism of 2,3-butanediol (BD) stereoisomer formation was examined with respect to the BD cycle. The enzymes acetylacetoin synthase, acetylacetoin reductase (AACR), and acetylbutanediol hydrolase (ABDH), which are part of the BD cycle, were found to be present in the cell-free extract of the bacterial strain Bacillus cereus YUF-4. Two kinds of acetylbutanediol (ABD) stereoisomers were produced in the reduction of acetylacetoin (AAC) by AACR, which were identified as having 3R,4R and 3S,4R configurations by NMR spectroscopy and an enzymic method. The two ABD formations were found to be catalyzed independently by two respective enzymes: the former was catalyzed by a NADPH-dependent AACR (3R,4R-ABD forming) and the latter by a NADH-dependent AACR (3S,4R-ABD forming). The 3R,4R-ABD was converted into R,R-BD and the 3S,4R-ABD into R,S-BD by intracellular ABDH. These findings demonstrated the existence of a new BD isomer formation mechanism derived from the BD cycle.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 1","pages":"Pages 79-83"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)80358-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91704875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from soil polluted with this contaminant 污染土壤中新分离的三氯乙烯降解菌的系统发育分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)80003-8

Satoshi Hanada , Toru Shigematsu , Katsutoshi Shibuya , Masahiro Eguchi , Takeshi Hasegawa , Fusako Suda , Yoichi Kamagata , Takahiro Kanagawa , Ryuichiro Kurane

{"title":"Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from soil polluted with this contaminant","authors":"Satoshi Hanada , Toru Shigematsu , Katsutoshi Shibuya , Masahiro Eguchi , Takeshi Hasegawa , Fusako Suda , Yoichi Kamagata , Takahiro Kanagawa , Ryuichiro Kurane","doi":"10.1016/S0922-338X(99)80003-8","DOIUrl":"https://doi.org/10.1016/S0922-338X(99)80003-8","url":null,"abstract":"<div>Five methanotrophs (strains 18-2, EB1, KSWIII, KSPIII and KSPIII) and three aromatic compound oxidizers (strains KP22, KP24 and KT1) were isolated from the natural field polluted with trichloroethylene (TCE). Phylogenetic analysis based on 16S rRNA gene sequence suggested that all of the isolates belonged to the class Proteobacteria. Two of the methanotrophic isolates, strains 18-2 and EB1, were closely related to Methylocystis sp. strain M in the α subclass of Proteobacteria with sequence similarities of 98.2–98.4%, while strains KSWIII, KSPIII and KSPII were akin to Methylomonas methanica in the γ subclass of Proteobacteria with sequence similarities of 97.8–98.1%. The aromatic compounds oxidizers, strains KP22, KP24 and KT1, were assigned to the β subclass of Proteobacteria, and classified as Bordetella sp. (97.2–97.8% sequence similarity to species of the genus Bordetella), Burkholderia cepacia (99.2%) and Ralstonia eutropha (99.4%), respectively. All isolates degraded TCE when cells were grown with the appropriate substrate, i.e., methane, phenol or toluene. Detailed kinetic analyses of their TCE degradation revealed that the rates of degradation (k1) among the isolates were 10–36 ml of TCE/mg of dry cell weight/h, and the transformation capacities (Tc) were 0.01–0.13 mg of TCE/mg of dry cell weight.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 6","pages":"Pages 539-544"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)80003-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91647852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 38