Journal of Fermentation and Bioengineering最新文献_第7页

Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale 海洋绿藻褐藻暗发酵生产乙醇的研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80031-7

Yoshiyuki Ueno , Norihide Kurano , Shigetoh Miyachi

引用次数: 136

Modification of metabolic pathways of Saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value 低pH条件下乳酸发酵乳酸脱氢酶基因的表达和丙酮酸脱羧酶基因的缺失对酿酒酵母代谢途径的修饰

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80131-1

Eri Adachi, Mikiko Torigoe, Minetaka Sugiyama, Jun-Ichi Nikawa, Kazuyuki Shimizu

{"title":"Modification of metabolic pathways of Saccharomyces cerevisiae by the expression of lactate dehydrogenase and deletion of pyruvate decarboxylase genes for the lactic acid fermentation at low pH value","authors":"Eri Adachi, Mikiko Torigoe, Minetaka Sugiyama, Jun-Ichi Nikawa, Kazuyuki Shimizu","doi":"10.1016/S0922-338X(98)80131-1","DOIUrl":"10.1016/S0922-338X(98)80131-1","url":null,"abstract":"<div>Extractive lactic acid fermentation has recently been paid a great deal of attention. The problem with such a process is, however, that only undissociated lactate can be extracted. Therefore, lactic acid fermentation at low pH values is desirable. In the present study, we modified the metabolism of yeast (not lactic acid producing bacteria often cultivated at pH of 6–7) by expressing the lactate dehydrogenase (LDH) gene for the production of lactate at low pH values. For this purpose, the plasmid pADNS which contains the ADH1 promoter was used as a host vector, and a heterologous gene region, cDNA-LDH-A (encoding bovine lactate dehydrogenase) digested from plasmid pLDH12 was digested and ligated into the aforementioned two host vectors. The resultant plasmids were then transformed into Saccharomyces cerevisiae DS37. Using this recombinant S. cerevisiae strain, several batch and fed-batch fermentations at aerobic, microaerobic, and anaerobic conditions were conducted at several pH values (4.5-3.5). Since the recombinant S. cerevisiae produced a considerable amount of ethanol as well as lactate (about 10 g/l), we disrupted several pyruvate decarboxylase (PDC) genes to suppress the ethanol formation. Among the PDC genes, PDC1, PDC5 and PDC6, PDC1 had the greatest effect on the cell growth and ethanol production. The plasmid which containing the LDH-A structure gene was then transformed into the mutant strain lacking the PDC1 gene. Cultivation of this strain improved the lactate yield from glucose (from 0.155 to 0.20) while suppressing ethanol formation (from 0.35 to 0.20).</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 3","pages":"Pages 284-289"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80131-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80971084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 106

Optimizing the culture conditions for higher inulinase production by Kluyveromyces sp. Y-85 and scaling-up fermentation 优化Kluyveromyces sp. Y-85高产菊粉酶的培养条件及扩大发酵规模

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)89011-4

Wenling Wei, Zhonghui Zheng, Yueying Liu, Xinsheng Zhu

引用次数: 38

Lipase-catalyzed kinetic resolution of 4-hydroxy-5-dodecynonitrile and its application to facile synthesis of a cupreous chafer beetle sex pheromone 脂肪酶催化4-羟基-5-十二烷基腈的动力学分解及其在铜金龟子性信息素合成中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80161-X

Ei-Ichiro Fukusaki , Shiro Satoda , Shuji Senda , Tetsuo Omata

引用次数: 3

Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene 含外源基因的温和气单胞菌脂肪酶的生产与固定化

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80140-2

Saovanee Dharmsthiti, Sudaporn Luchai

引用次数: 3

Cloning and nucleotide sequencing of genes encoding Mn-superoxide dismutase and class II fumarase from Thermus aquaticus YT-1 水热菌mn -超氧化物歧化酶和II类富马酸酶基因的克隆与序列分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80028-7

Hidemasa Motoshima , Etsuo Minagawa, Fuji Tsukasaki, Shuichi Kaminogawa

{"title":"Cloning and nucleotide sequencing of genes encoding Mn-superoxide dismutase and class II fumarase from Thermus aquaticus YT-1","authors":"Hidemasa Motoshima , Etsuo Minagawa, Fuji Tsukasaki, Shuichi Kaminogawa","doi":"10.1016/S0922-338X(98)80028-7","DOIUrl":"10.1016/S0922-338X(98)80028-7","url":null,"abstract":"<div>A 6955-bp sequence of a PstI-HindIII DNA fragment containing the manganese-superoxide dismutase (MnSOD) gene of Thermus aquaticus YT-1 was determined. The gene (sod) encoded a polypeptide consisting of 204 amino acid residues (the mature enzyme without the initiation methionine) with a calculated Mr of 22,773. The deduced amino acid sequence of the sod gene showed 92% identity to that of Thermus thermophilus HB8 determined chemically. The sod gene was well expressed in Escherichia coli and a heat-stable active enzyme was produced. An open reading frame encoding fumarase was found 91 bp upstream of the MnSOD gene in tandem form. The fum gene encoded a polypeptide consisting of 466 amino acid residues with a calculated Mr of 50,950. The deduced amino acid sequence of the fum gene product has similarity to that of the fumC of E. coli (57% identity), suggesting that this gene encodes a class II type fumarase.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 21-27"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80028-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78550134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Alanine racemase gene fragments as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods 丙氨酸消旋酶基因片段在食品中嗜脂嗜热芽孢杆菌和嗜冷糖芽孢杆菌检测中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80005-6

Yoko Okubo, Kumio Yokoigawa, Hiroyasu Kawai

{"title":"Alanine racemase gene fragments as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods","authors":"Yoko Okubo, Kumio Yokoigawa, Hiroyasu Kawai","doi":"10.1016/S0922-338X(98)80005-6","DOIUrl":"10.1016/S0922-338X(98)80005-6","url":null,"abstract":"<div>Alanine racemase gene fragments containing non-conserved regions of the gene were evaluated as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods. The gene fragments were amplified from genomic DNA of each bacterium by polymerase chain reaction with degenerate oligonucleotide primers, and labeled with digoxigenin as probes for detecting each bacterium. Foods and bacteria were treated at 25°C for 10 min in 0.1 N NaOH containing 0.5% SDS before being directly spotted onto nylon membranes for DNA hybridization. When the specificities of the probes were analyzed using a total of 86 strains (23 genera and 65 species) of bacteria including 29 Bacillus strains (20 species), each probe was specific for the respective target bacteria. A variety of foods inoculated with B. stearothermophilus or B. psychrosaccharolyticus were positive as determined by hybridization with the respective probe, whereas uninoculated foods were negative. The alanine racemase gene fragments could be used as specific probes for detecting B. stearothermophilus and B. psychrosaccharolyticus in foods.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 6","pages":"Pages 559-563"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80005-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75789087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved ε-poly-l-lysine production of an S-(2-aminoethyl)-l-cysteine resistant mutant of Streptomyces albulus 白链霉菌S-(2-氨基乙基)-l-半胱氨酸抗性突变体ε-聚赖氨酸产量的提高

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)80023-3

引用次数: 0

High Production of Pyoluteorin and 2, 4-Diacetylphloroglucinol by Pseudomonas fluorescens S272 Grown on Ethanol as a Sole Carbon Source 以乙醇为唯一碳源培养荧光假单胞菌S272高产吡咯啉和2,4 -二乙酰间苯三酚

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)80006-3

Zhan Yuan, S. Cang, M. Matsufuji, K. Nakata, Y. Nagamatsu, A. Yoshimoto

引用次数: 44

Phylogenetic analysis of trichloroethylene-degrading bacteria newly isolated from soil polluted with this contaminant 污染土壤中新分离的三氯乙烯降解菌的系统发育分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)80003-8

S. Hanada, T. Shigematsu, Katsutoshi Shibuya, M. Eguchi, Takeshi Hasegawa, F. Suda, Y. Kamagata, T. Kanagawa, R. Kurane

引用次数: 40