Journal of Fermentation and Bioengineering最新文献_第7页

Cloning, nucleotide sequence, and transcriptional analysis of Aspergillus aculeatus no. F-50 cellobiohydrolase I (cbhI) gene 刺曲霉的克隆、核苷酸序列及转录分析。F-50纤维生物水解酶I (cbhI)基因

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80345-5

Goro Takada, Takashi Kawaguchi, Jun-Ichi Sumitani, Motoo Arai

{"title":"Cloning, nucleotide sequence, and transcriptional analysis of Aspergillus aculeatus no. F-50 cellobiohydrolase I (cbhI) gene","authors":"Goro Takada, Takashi Kawaguchi, Jun-Ichi Sumitani, Motoo Arai","doi":"10.1016/S0922-338X(97)80345-5","DOIUrl":"https://doi.org/10.1016/S0922-338X(97)80345-5","url":null,"abstract":"<div>The cbhI gene, coding for a major cellobiohydrolase (CBHI) of Aspergillus aculeatus, was cloned and sequenced. The gene consists of 1620-bp and encodes a protein containing 540 amino acids with a calculated molecular mass of 56,723 Da. CBHI, composed of an N-terminal catalytic domain belonging to family 7 of the glycosyl hydrolases, and a C-terminal cellulose-binding domain (CBD) belonging to family I of the CBDs, showed high similarity with other fungal CBHIs, especially with that of Penicillium janthinellum. The cbhI gene transcription start points in A. aculeatus were defined by primer extension, and the putative promoter sequence was analyzed. This sequence was found to be closely related to the consensus sequences of various fungal genes. Transcription analysis by ribonuclease protection assay revealed that the cbhI gene is induced by low-molecular-weight cellooligosaccharide and repressed by glucose. The results emphasize the possibility that in the A. aculeatus cellulase system, cellobiose is the true inducer and the role of the cbhI gene lies within the cascade regulating cellulase induction.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 1","pages":"Pages 1-9"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)80345-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91704883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots 考虑毛状根高密度培养过程中生物量变化的生物量估算程序

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80095-0

Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu

{"title":"Procedure for biomass estimation considering the change in biomass volume during high density culture of hairy roots","authors":"Kyung-Hee Jung, Sang-Soo Kwak, Jang R. Liu","doi":"10.1016/S0922-338X(98)80095-0","DOIUrl":"10.1016/S0922-338X(98)80095-0","url":null,"abstract":"<div>The biomass concentration in a bubble column reactor was estimated by analyzing the conductivity change during high-density culture of Catharanthus roseus hairy roots. The accumulated biomass in the reactor was estimated from the amount of nutrients consumed, which were present in the liquid medium. Liquid medium, consisting of water and nutrients, was incorporated into the biomass, in proportion to the growth of hairy roots, consequently the ratio of the volume of liquid medium to the volume of the biomass decreased continuously. To evaluate nutrient consumption, the change in biomass volume in the reactor was introduced as a new parameter for biomass estimation. After 45 d of culture, the final concentration of hairy roots was 46.5 g dry wt./l, of which the biomass volume is about 40% of the total culture volume of the reactor. A conventional conductivity method, in which the volume change of the liquid medium was not considered, resulted in a deviation (55.76 g dry wt./l) of about 20% from the actual biomass. This procedure yielded a value of 47.1 g dry wt./l of hairy roots, which is a nearly accurate estimation of the biomass concentration. This result suggests that the volume reduction of liquid medium should be considered for biomass estimation and process control for high-density plant cell cultures.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 4","pages":"Pages 454-457"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80095-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91340823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Purification and characterization of component B of a soluble methane monooxygenase from Methylocystis sp. M 甲基藻可溶性甲烷单加氧酶B组分的纯化及特性研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80350-9

Y. Shinohara, H. Uchiyama, O. Yagi, I. Kusakabe

引用次数: 14

Ethanol-induced water stress and fungal growth 乙醇诱导水分胁迫和真菌生长

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80150-5

John E. Hallsworth, Yoshiyuki Nomura, Masayoshi Iwahara

{"title":"Ethanol-induced water stress and fungal growth","authors":"John E. Hallsworth, Yoshiyuki Nomura, Masayoshi Iwahara","doi":"10.1016/S0922-338X(98)80150-5","DOIUrl":"10.1016/S0922-338X(98)80150-5","url":null,"abstract":"<div>Fungal growth inhibition by ethanol was compared with that caused by five other agents of water stress (at 25, 40 and 42.5°C), using Aspergillus oryzae. Ethanol, KCl, glycerol, glucose, sorbitol, and polyethylene glycol 400 were incorporated into media at concentrations corresponding to water activity (aw) values in the range 1 to 0.75. Generally, as temperature increased there was a decrease in the aw value at which optimum growth occurred. The aw limit for growth on KCl, glycerol, glucose, sorbitol, or polyethylene glycol 400 media was about 0.85, regardless of temperature. However, the aw limit for growth on ethanol media varied between 0.97 and 0.99 aw and was temperature-dependent. Water stress accounted for up to 31, 18 and 6% of growth inhibition by ethanol at 25, 40, and 42.5°C, respectively. For media containing ethanol, the decrease in growth rate per unit of aw reduction was greater as temperature increased. However, ethanol-induced water stress remained constant regardless of temperature, suggesting that other inhibitory effects of ethanol are closely temperature-dependent. Water stress may account for considerably more than 30% of growth inhibition by ethanol in cells that remain metabolically active at higher ethanol concentrations.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 5","pages":"Pages 451-456"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80150-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90539413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 68

The importance of ionic strength as a parameter in screening peptide ligands from a phage display library 离子强度作为筛选噬菌体展示文库中肽配体的参数的重要性

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80093-7

Yoshio Katakura, Eun Tae Lim, Setsuo Tsujii, Takeshi Omasa, Ken-Ichi Suga

引用次数: 2

Purification and characterization of β-N-acetylhexosaminidase from Streptomyces sp. no. 499 链霉菌β- n -乙酰己糖氨酸酶的纯化及特性研究。499

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80110-4

引用次数: 0

Key work index 关键工作指标

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)89021-7

引用次数: 0

Improved ε-poly-l-lysine production of an S-(2-aminoethyl)-l-cysteine resistant mutant of Streptomyces albulus 白链霉菌S-(2-氨基乙基)-l-半胱氨酸抗性突变体ε-聚赖氨酸产量的提高

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)80023-3

引用次数: 0

A dense cell culture system for aerobic microorganisms using a shaken ceramic membrane flask with surface aeration 一种使用带有表面曝气的摇摇陶瓷膜烧瓶的好氧微生物密集细胞培养系统

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)86771-2

Yuya Kamoshita, Ryo Ohashi, Takahiro Suzuki

{"title":"A dense cell culture system for aerobic microorganisms using a shaken ceramic membrane flask with surface aeration","authors":"Yuya Kamoshita, Ryo Ohashi, Takahiro Suzuki","doi":"10.1016/S0922-338X(97)86771-2","DOIUrl":"10.1016/S0922-338X(97)86771-2","url":null,"abstract":"<div>Aeration of the head space in a shaken ceramic membrane flask (SCM flask) capped with a cotton plug was found to be essential for increasing cell concentration and viability of aerobic microorganisms. Ventilation through the cotton plug cap was insufficient for satisfying the oxygen demand of aerobically growing cells in the SCM flask. Dissolved oxygen concentration (DO) in the culture supernatant of Escherichia coli in batch culture using the SCM flask dropped to nearly 0 ppm when the cell concentration reached 1 g/l, while CO2 concentration in the head space of the SCM flask increased quickly to nearly 20% owing to accumulation of the evolved CO2. In contrast, the oxygen transfer coefficient in a SCM flask shaken at 230 rpm was as high as 220 h−1, compared to that in a stirred type jar fermentor. Consequently, aeration of the head space of the SCM flask was found to be sufficient for supplying oxygen by vigorous waves created in the culture broth due to the reciprocal shaking action. Using the SCM flask, E. coli cell mass reached 84 g/l in 40 h with aeration of the head space with oxygen-enriched gas. The SCM flask with aeration to the head space enabled an increase in the concentration and productivity of viable cells to be readily achieved by continuously replenishing the culture supernatant with oxygen in conjunction with the removal of the evolved CO2.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 2","pages":"Pages 218-222"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)86771-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74438828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Excretion of glycerol by the marine Chlamydomonas sp. strain W-80 in high CO2 cultures 海洋衣藻属菌株W-80在高CO2培养中的甘油排泄

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80367-4

Hitoshi Miyasaka , Yosuke Ohnishi , Toru Akano , Kiyomi Fukatsu , Tadashi Mizoguchi , Kiyohito Yagi , Isamu Maeda , Yoshiaki Ikuta , Hiroyo Matsumoto , Norio Shioji , Yoshiharu Miura

引用次数: 32