Journal of Fermentation and Bioengineering最新文献

Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae 酿酒酵母聚半乳糖醛酸酶基因的克隆与表达

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80139-6

Naoto Hirose , Masao Kishida , Haruhiko Kawasaki , Takuo Sakai

引用次数: 25

Purification and characterization of a novel β-glucosidase from Clavibacter michiganense that hydrolyzes glucosyl ester linkage in steviol glycosides 水解甜菊糖苷中葡萄糖酯链的新型β-葡萄糖苷酶的纯化与表征

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)86761-X

Hirofumi Nakano , Katsuyuki Okamoto , Tsuneya Yatake , Taro Kiso , Sumio Kitahata

{"title":"Purification and characterization of a novel β-glucosidase from Clavibacter michiganense that hydrolyzes glucosyl ester linkage in steviol glycosides","authors":"Hirofumi Nakano , Katsuyuki Okamoto , Tsuneya Yatake , Taro Kiso , Sumio Kitahata","doi":"10.1016/S0922-338X(97)86761-X","DOIUrl":"10.1016/S0922-338X(97)86761-X","url":null,"abstract":"<div>Clavibacter michiganense was identified as a microorganism that hydrolyzed the glucosyl ester linkages at site 19 of steviol glycosides. An enzyme that catalyzes the hydrolysis was purified from the cell-free extract using streptomycin treatment, ammonium sulfate fractionation, Q Sepharose anion exchange chromatography, Sephacryl S-100 gel filtration, and Ether Toyopearl hydrophobic chromatography. The purified enzyme migrated as a single protein band in polyacrylamide gel electrophoresis in the presence and absence of sodium dodecyl sulfate, and isoelectric focusing. The molecular mass was estimated to be approximately 65 kDa, both by gel filtration and sodium dodecyl sulfate/polyacrylamide gel electrophoresis. An isoelectric point, pI, of 4.6, was obtained using isoelectric focusing. The enzyme was most active at around pH 7.5 and at 45°C, and was stable between pH 6–10 and below 40°C. Both Hg2+ and p-chloromercuric benzoate inhibited activity. The enzyme hydrolyzed glucosyl ester linkages at site 19 of rebaudioside A, stevioside, rubusoside, and steviol monoglucosyl ester, although it did not cleave 13-O-linked glucosyl residue of rubusoside and steviol monoside. A transglucosylation product having a cellobiosyl residue at site 19 was formed when rubusoside was used as a glucosyl donor and acceptor. The enzyme hydrolyzed glucosidic linkages in p-nitrophenyl β-glucoside faster than glucosyl ester linkages in the steviol glycosides. It also acted on phenyl β-glucoside and salicin, and faintly on sophorobiose and cellobiose. These results indicate that the enzyme is a novel β-glucosidase that hydrolyzes ester linkages.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)86761-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75809998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Novel anthraquinones from stationary cultures of Fusarium oxysporum 尖孢镰刀菌固定培养物中新的蒽醌类化合物

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80077-9

Robert A. Baker, James H. Tatum

引用次数: 76

SSU1-R, a sulfite resistance gene of wine yeast, is an allele of SSU1 with a different upstream sequence 葡萄酒酵母抗亚硫酸盐基因SSU1- r是SSU1上游序列不同的等位基因

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80146-3

Nami Goto-Yamamoto, Kazuyoshi Kitano , Kunio Shiki , Yuichi Yoshida , Takashi Suzuki , Tomoko Iwata , Yoshiharu Yamane , Shodo Hara

{"title":"SSU1-R, a sulfite resistance gene of wine yeast, is an allele of SSU1 with a different upstream sequence","authors":"Nami Goto-Yamamoto, Kazuyoshi Kitano , Kunio Shiki , Yuichi Yoshida , Takashi Suzuki , Tomoko Iwata , Yoshiharu Yamane , Shodo Hara","doi":"10.1016/S0922-338X(98)80146-3","DOIUrl":"10.1016/S0922-338X(98)80146-3","url":null,"abstract":"<div>A sulfite resistance gene, SSU1-R, was cloned from a highly sulfite-resistant strain of Saccharomyces cerevisiae, Y-9, which was isolated as a contaminant from a wine must. The coding region sequence of SSU1-R was almost identical to that of SSU1, the gene that has been shown to be responsible for sulfite resistance. Although SSU1 is located on chromosome XVI of S. cerevisiae, the upstream region of SSU1-R was homologous with a sequence of chromosome VIII. In our Northern analysis, an intense band was detected in Y-9 and K1-V1116 strains, both of which exhibit strong sulfite resistance, with and without sulfite in the medium. On the other hand, under the same experimental conditions, no band was detected in OC-2, a strain that exhibits weak sulfite resistance. Physical mapping of SSU1/SSU1-R showed that this sequence was on chromosome VIII of Y-9 and the two wine strains, K1-V1116 and WE14. OC-2 and twenty-three other wine, sake, beer, shochu (Japanese distilled liquor), alcohol, bakery, and laboratory strains, had this sequence on chromosome XVI, and four other wine strains had it on both chromosomes. Thus, the difference in the upstream sequence of SSU1/SSU1-R seems to cause differences in the transcription rates and degree of sulfite resistance.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80146-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81495002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 72

Hydrogen peroxide as an antibacterial factor in zinc oxide powder slurry 过氧化氢在氧化锌粉浆中的抑菌作用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80165-7

Jun Sawai , Shinobu Shoji , Hideo Igarashi , Atsushi Hashimoto , Takao Kokugan , Masaru Shimizu , Hiromitsu Kojima

引用次数: 380

Performance of photosynthetic electrochemical cells using immobilized Anabaena variabilis M-3 in discharge/culture cycles 固定化变水藻M-3在放电/培养循环中的光合电化学电池性能

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80106-2

Tatsuo Yagishita, Shigeki Sawayama, Ken-Ichiro Tsukahara, Tomoko Ogi

引用次数: 40

Inhibitory effects of acetic acid on respiration and growth of Zygosaccharomyces rouxii 醋酸对柔糖酵母菌呼吸和生长的抑制作用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)86770-0

Kazuhiro Kusumegi , Hidetsugu Yoshida , Sachiko Tomiyama

引用次数: 16

Papain-catalyzed synthesis of aspartame precursor: A comparison with thermolysin 木瓜蛋白酶催化合成阿斯巴甜前体:与热溶酶的比较

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80351-0

H. Nakaoka, Y. Miyajima, K. Morihara

引用次数: 7

Response of an anaerobic granular sludge to chlorinated aliphatic hydrocarbons in different conditions 厌氧颗粒污泥在不同条件下对氯化脂肪烃的反应

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80118-9

Nuria Rodríguez, JoséL. Sanz

{"title":"Response of an anaerobic granular sludge to chlorinated aliphatic hydrocarbons in different conditions","authors":"Nuria Rodríguez, JoséL. Sanz","doi":"10.1016/S0922-338X(98)80118-9","DOIUrl":"10.1016/S0922-338X(98)80118-9","url":null,"abstract":"<div>In spite of its importance, little attention has been paid thus far to the toxicity of chlorinated aliphatic hydrocarbons under methanogenic conditions. The effect of several parameters which can affect the toxicity of CHCl3, CCl4, C2H2Cl4 and trans-C2H2Cl2 on the acetoclastic methanogenic activity of anaerobic sludge was studied. The following conclusions were derived: (i) The extend of inhibition produced is a function of the toxicant concentration, the type and the amount of the biomass. A lag-period was detected for the inhibition produced by CHCl3 and trans-C2H2Cl2, while for the other compounds the inhibitory effect was instant. (ii) The recovery time for an anaerobic granular sludge exposed to different toxicants was a function of the extent of inhibition and the exposure time, and seemed to be independent of the toxicants used. (iii) The possible acclimatization of an anaerobic sludge to continuous exposure to a toxicant was dependent on the nature of the toxicant. The time required to acquire an activity similar to that of untreated controls was dependent on the concentration of the toxicant used. In our case adaptation was only obtained for CCl4.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80118-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77963420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Cloning and nucleotide sequence of the nitric oxide reductase locus in Paracoccus denitrificans IFO 12442 反硝化副球菌IFO 12442一氧化氮还原酶位点的克隆及核苷酸序列分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80158-X

Kiyohito Murai, Katsuhide Miyake, Jun Andoh, Shinji Iijima

{"title":"Cloning and nucleotide sequence of the nitric oxide reductase locus in Paracoccus denitrificans IFO 12442","authors":"Kiyohito Murai, Katsuhide Miyake, Jun Andoh, Shinji Iijima","doi":"10.1016/S0922-338X(98)80158-X","DOIUrl":"10.1016/S0922-338X(98)80158-X","url":null,"abstract":"<div>A 5.2-kb DNA fragment containing the upstream region of the nitrite reductase gene (nirS) was cloned from Paracoccus denitrificans IFO 12442 and its DNA sequence was determined. In this fragment, four open reading frames (ORFs) were observed. Among these, two ORFs, located 3 kb upstream of the nirS gene were found to encode nitric oxide reductase (norC, norB) by homology analysis. The norC and norB encoded cytochrome c and b subunits of the enzyme, and the predicted molecular weights of the protein products were 17 kDa (150 amino acid residues) and 52.5 kDa (462 amino acid residues), respectively. The other two open reading frames, designated ORF1 (73 kDa, 681 amino acid residues) and ORF2 (32.5 kDa, 304 amino acid residues), were found between the nir and nor genes. The deduced amino acid sequence of ORF1 showed high similarity (35% identity) to that of NosR which is known to be a transcriptional regulatory protein for the N2O reductase gene cluster of Pseudomonas stutzeri. Since the ORF1 protein has a well conserved cysteine cluster and 5 hydrophobic transmembrane repeats similar to those of the NosR protein, ORF1 probably functions as a membrane bound sensor for denitrification. Northern blot analysis indicated that the ORF1–2 and norCB regions are probably transcribed as independent operons.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80158-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81770126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2