{"title":"酿酒酵母聚半乳糖醛酸酶基因的克隆与表达","authors":"Naoto Hirose , Masao Kishida , Haruhiko Kawasaki , Takuo Sakai","doi":"10.1016/S0922-338X(98)80139-6","DOIUrl":null,"url":null,"abstract":"<div><p>An endo-polygalacturonase gene was cloned from a mutant of <em>Saccharomyces cerevisiae</em> that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of <em>S. cerevisiae</em> that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80139-6","citationCount":"25","resultStr":"{\"title\":\"Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae\",\"authors\":\"Naoto Hirose , Masao Kishida , Haruhiko Kawasaki , Takuo Sakai\",\"doi\":\"10.1016/S0922-338X(98)80139-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>An endo-polygalacturonase gene was cloned from a mutant of <em>Saccharomyces cerevisiae</em> that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of <em>S. cerevisiae</em> that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80139-6\",\"citationCount\":\"25\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X98801396\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X98801396","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae
An endo-polygalacturonase gene was cloned from a mutant of Saccharomyces cerevisiae that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of S. cerevisiae that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
