{"title":"极端嗜热菌HB27富马酸酶和超氧化物歧化酶基因的克隆与序列分析","authors":"Takehide Kosuge, Kaho Umehara, Takayuki Hoshino","doi":"10.1016/S0922-338X(98)80045-7","DOIUrl":null,"url":null,"abstract":"<div><p>Class II fumarase (<em>fumC</em>) and Mn-containing superoxide dismutase (<em>sodA</em>) genes were cloned and sequenced from <em>Thermus thermophilus</em> HB27. The <em>fumC</em> and <em>sodA</em> genes existed in tandem. Comparison of the DNA and amino acid sequences of the <em>funC-sodA</em> regions between <em>T. thermophilus</em> HB27 and <em>Thermus aquaticus</em> YT1 revealed that coding regions showed high homology but noncoding regions were less homologous between the two strains. The deduced amino acid sequences of <em>T. thermophilus fumC</em> and <em>sodA</em> genes showed 58.2% and 53.2% identities to those of the corresponding <em>Escherichia coli</em> genes, respectively. The <em>T. thermophilus fumC</em> gene was expressed in <em>E. coli</em> and the heat-resistant fumarase activity was detected at 70°C.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80045-7","citationCount":"3","resultStr":"{\"title\":\"Cloning and sequence analysis of fumarase and superoxide dismutase genes from an extreme thermophile Thermus thermophilus HB27\",\"authors\":\"Takehide Kosuge, Kaho Umehara, Takayuki Hoshino\",\"doi\":\"10.1016/S0922-338X(98)80045-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Class II fumarase (<em>fumC</em>) and Mn-containing superoxide dismutase (<em>sodA</em>) genes were cloned and sequenced from <em>Thermus thermophilus</em> HB27. The <em>fumC</em> and <em>sodA</em> genes existed in tandem. Comparison of the DNA and amino acid sequences of the <em>funC-sodA</em> regions between <em>T. thermophilus</em> HB27 and <em>Thermus aquaticus</em> YT1 revealed that coding regions showed high homology but noncoding regions were less homologous between the two strains. The deduced amino acid sequences of <em>T. thermophilus fumC</em> and <em>sodA</em> genes showed 58.2% and 53.2% identities to those of the corresponding <em>Escherichia coli</em> genes, respectively. The <em>T. thermophilus fumC</em> gene was expressed in <em>E. coli</em> and the heat-resistant fumarase activity was detected at 70°C.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80045-7\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X98800457\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X98800457","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Cloning and sequence analysis of fumarase and superoxide dismutase genes from an extreme thermophile Thermus thermophilus HB27
Class II fumarase (fumC) and Mn-containing superoxide dismutase (sodA) genes were cloned and sequenced from Thermus thermophilus HB27. The fumC and sodA genes existed in tandem. Comparison of the DNA and amino acid sequences of the funC-sodA regions between T. thermophilus HB27 and Thermus aquaticus YT1 revealed that coding regions showed high homology but noncoding regions were less homologous between the two strains. The deduced amino acid sequences of T. thermophilus fumC and sodA genes showed 58.2% and 53.2% identities to those of the corresponding Escherichia coli genes, respectively. The T. thermophilus fumC gene was expressed in E. coli and the heat-resistant fumarase activity was detected at 70°C.
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