{"title":"Molecular cloning and expression of a polygalacturonase gene in Saccharomyces cerevisiae","authors":"Naoto Hirose , Masao Kishida , Haruhiko Kawasaki , Takuo Sakai","doi":"10.1016/S0922-338X(98)80139-6","DOIUrl":null,"url":null,"abstract":"<div><p>An endo-polygalacturonase gene was cloned from a mutant of <em>Saccharomyces cerevisiae</em> that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of <em>S. cerevisiae</em> that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 3","pages":"Pages 332-334"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80139-6","citationCount":"25","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X98801396","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 25
Abstract
An endo-polygalacturonase gene was cloned from a mutant of Saccharomyces cerevisiae that can produce a polygalacturonase, and the nucleotide sequence was identified. The polygalacturonase gene consists of 1086 bp. When the polygalacturonase gene was introduced into a strain of S. cerevisiae that cannot produce a polygalacturonase, the transformant secreted a polygalacturonase identical to that produced by the polygalacturonase-producing mutant.