Journal of Fermentation and Bioengineering最新文献_第6页

Enhancement of transfection efficiency using ligand-modified lipid vesicles 利用配体修饰脂质囊泡提高转染效率

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80075-5

Jun You, Masamichi Kamihira, Shinji Iijima

引用次数: 8

High cell density culture of Rhodococcus rhodochrous by pH-stat feeding and dibenzothiophene degradation ph -稳态饲养和二苯并噻吩降解法培养红红红球菌

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)85685-1

Hiroyuki Honda, Hiroyasu Sugiyama, Ikuo Saito, Takeshi Kobayashi

{"title":"High cell density culture of Rhodococcus rhodochrous by pH-stat feeding and dibenzothiophene degradation","authors":"Hiroyuki Honda, Hiroyasu Sugiyama, Ikuo Saito, Takeshi Kobayashi","doi":"10.1016/S0922-338X(97)85685-1","DOIUrl":"10.1016/S0922-338X(97)85685-1","url":null,"abstract":"<div>A high cell density culture of Rhodococcus rhodochrous IGTS8 was investigated. Acetic acid was one of the most suitable carbon sources for cell growth and sulfate ion was more suitable than dibenzothiophene (DBT) as a sulfur source. Fed-batch culture was conducted in a 1-l jar fermentor with FB medium containing acetic acid and sulfate ion as carbon and sulfur sources. Cell growth was found to be inhibited when the concentrations of acetic acid and ammonium ion were above 3 g/l. To control the concentrations of the two components below 3 g/l, a mixture of acetic acid and ammonium acetate was supplied by means of pH-stat feeding. As a result, a cell concentration of 33 g dry cells/l was obtained after 28-h cultivation. When the cells obtained were incubated in a fresh medium containing DBT as a substrate, hydroxybiphenyl (HBP), which is the end-product of the DBT degradation pathway, was detected and its production rate gradually increased with incubation time. Incubation for 3 to 4 h was enough for the full induction of DBT-degrading enzymes, and the specific production rate of HBP was about 6.1 mmol/kg dry cells/h. A two-phase cultivation (cell growth phase and induction phase) is proposed in order to obtain a high cell density and full induction of DBT-degrading enzymes.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 3","pages":"Pages 334-338"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)85685-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81553160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Microbial conversion of d-xylose to xylitol d-木糖转化为木糖醇的微生物过程

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80026-3

Eleonora Winkelhausen, Slobodanka Kuzmanova

{"title":"Microbial conversion of d-xylose to xylitol","authors":"Eleonora Winkelhausen, Slobodanka Kuzmanova","doi":"10.1016/S0922-338X(98)80026-3","DOIUrl":"10.1016/S0922-338X(98)80026-3","url":null,"abstract":"<div>Xylitol, a five carbon sugar alcohol, occurs widely in nature but it is also a normal intermediate in human metabolism. As an alternative sweetener, it is recommended for diabetics and for the prevention of dental caries. Xylitol is currently produced chemically on a large scale. Microbial production is lately becoming more attractive since the downstream processing is expected to be cheaper. Among microorganisms, yeasts are the best xylitol producers, particularly those belonging to the genus Candida. The key enzymes for xylitol production in yeasts are d-xylose reductase which, using either NADH or NADPH, reduces d-xylose to xylitol, and predominantly, NAD-linked xylitol dehydrogenase which reoxidizes xylitol to d-xylulose. Xylitol accumulation in yeasts is sensitive to environmental conditions such as nutrition, temperature, pH, inoculum, substrate and aeration, with the last two being critical for yeast growth and fermentation. Hemicellulosic hydrolysates derived from hardwood and particularly from agricultural residues, such as sugar cane bagasse, corn cobs, wheat and rice straw, are used as feedstock for xylitol production. Due to the presence of inhibitory components, some of the hydrolysates have to be treated prior to microbial utilization. The most investigated types of processes have been batch ones, although fed-batch and immobilized systems have been characterized by the highest yields and productivities. Apart from the naturally occurring yeasts, recombinant strains of Saccharomyces cerevisiae in free and immobilized form were also investigated for xylitol production.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 1-14"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80026-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89478073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 348

Succinoglycan production by Agrobacterium tumefaciens 农杆菌产琥珀聚糖的研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80083-4

Miroslav Stredansky , Elena Conti , Claudia Bertocchi , Maria Matulova , Flavio Zanetti

引用次数: 25

Key work index 关键工作指标

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(99)89021-7

引用次数: 0

Effect of electric current on growth and alcohol production by yeast cells 电流对酵母细胞生长和产醇的影响

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)86778-5

Kotoyoshi Nakanishi, Hiroharu Tokuda, Takahiko Soga, Takahiro Yoshinaga, Masahisa Takeda

引用次数: 54

Control of cell growth rate by sugar feeding based on CO2 production rate 以CO2产率为基础，通过喂糖控制细胞生长速率

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(97)85690-5

Mutsumi Takagi, Fumihiro Ishimura, Isao Fujimatsu

{"title":"Control of cell growth rate by sugar feeding based on CO2 production rate","authors":"Mutsumi Takagi, Fumihiro Ishimura, Isao Fujimatsu","doi":"10.1016/S0922-338X(97)85690-5","DOIUrl":"10.1016/S0922-338X(97)85690-5","url":null,"abstract":"<div>Sugar feeding based on the CO2 production rate was investigated for the control of the cell growth rate during cephalosporin C fermentation in which sugar concentration was the growth limiting factor. The rates of cell growth and sugar consumption were apparently influenced by the sugar feed rate, as the sugar concentration in the broth was kept at approximately 1 g/l during the cultivation. The ratio of the CO2 production rate to the sugar consumption rate was maintained almost constant after 40 h cultivation. Stepwise alteration in sugar feed rate after the culture time of 50 h caused a change in the CO2 production rate within 15 min. These results indicated that the CO2 production rate could be used as an effective parameter of sugar consumption and cell growth rates. Consequently, a control strategy was developed that involved the control of sugar feed rate so that a CO2 production rate profile corresponded to a preset standard profile. For the half the usual amount of inoculum, the sugar feeding control system enabled the cell concentration to increase faster to that in the usual inoculum. The cell concentration deviation during exponential growth phase among several batches, decreased to half using this sugar feeding control system compared to the culture without such control. These results indicated that the sugar feeding control system developed in this report was found to be efficient for control of cell growth.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 3","pages":"Pages 354-357"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)85690-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79881908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of chitosan-conjugated magnetite for magnetic cell separation 壳聚糖偶联磁铁矿的研制

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80060-3

Hiroyuki Honda, Atsushi Kawabe, Masashige Shinkai, Takeshi Kobayashi

{"title":"Development of chitosan-conjugated magnetite for magnetic cell separation","authors":"Hiroyuki Honda, Atsushi Kawabe, Masashige Shinkai, Takeshi Kobayashi","doi":"10.1016/S0922-338X(98)80060-3","DOIUrl":"10.1016/S0922-338X(98)80060-3","url":null,"abstract":"<div>For the purpose of separating microorganisms from culture broth by magnetic force, magnetic particles were conjugated with a polymer to produce polymer-conjugated magnetite (polymer-mag). Among 4 preparation methods investigated—aminosilane coupling, glycidylsilane coupling, crosslinking, and co-precipitation—, polymer-mag prepared by co-precipitation showed the highest cell recovery and high dispersibility. When various cationic, anionic, and nonionic polymers were used to prepare polymer-mag and applied to the magnetic separation for Escherichia coli, magnetite conjugated with chitosan (chitosan-mag) gave the highest cell recovery. In addition, E. coli cells could be recovered as the precipitant only 1 min after chitosan-mag was added to a cell suspension, and a clarified supernatant was obtained. The amount of E. coli cells adsorbed to the chitosan-mag was about 1 g-dry cells/g-chitosan-mag, and cell recovery of over 90% was attained in wide pH range from 3.0 to 7.0. Of 12 microorganisms tested, 4 could be recovered with chitosan-mag at recovery levels above 90%, and the adsorbed amounts exceeded 0.5 g-dry cells/g-chitosan-mag. Differences in adsorbed amounts were considered to be mainly due to the different zeta potential of the microorganisms tested.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 2","pages":"Pages 191-196"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80060-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84452104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

Interaction between homoacetogens and methanogens in lake sediments 湖泊沉积物中同质产氧菌与产甲烷菌的相互作用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80153-0

Jiunn-Jyi Lay , Yu-You Li , Tatsuya Noike

{"title":"Interaction between homoacetogens and methanogens in lake sediments","authors":"Jiunn-Jyi Lay , Yu-You Li , Tatsuya Noike","doi":"10.1016/S0922-338X(98)80153-0","DOIUrl":"10.1016/S0922-338X(98)80153-0","url":null,"abstract":"<div>The interaction between homoacetogens and methanogens in lake sediments was investigated using hydrogen consumption as an indicator. Sediments samples were obtained from Lake Izunuma, Miyagi prefecture, Japan, a wintering place for migratory birds from Siberia. A batch experiment using <math><mtext>H</mtext><msub><mi></mi><mn>2</mn></msub><mtext>CO</mtext><msub><mi></mi><mn>2</mn></msub></math> as a substrate was conducted to determine the acetate generation and methane production potential of the sediments. Incubation for 4 d at 37°C gave the following stoichiometric equation: 88H2 + 39HCO3− + 22H+ → 17CH3COO− + 5CH4 + 83H2O. The activities, νm, of hydrogen-utilizing homoacetogens and methanogens respectively ranged from 3.2 to 48 and from 1.8 to 3.2 mgCOD·gVSS−1·h−1. The population of hydrogen-utilizing homoacetogens was determined to be 2.6 × 108 MPN·gVSS−1, which was approximately two orders of magnitude higher than that of hydrogen-utilizing methanogens. The results suggest that homoacetogens in the sediments functioned not only as hydrogen consumers but also as major degraders of organic matter, forming acetate as the major reduction product.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 5","pages":"Pages 467-471"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80153-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76373597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 44

Synthesis of uridine 5′-monophosphate glucose as an inhibitor of UDP-glucose pyrophosphorylase 尿苷5′-单磷酸葡萄糖作为udp -葡萄糖焦磷酸化酶抑制剂的合成

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 Epub Date: 2002-02-27 DOI: 10.1016/S0922-338X(98)80052-4

Ken-Ichi Fujita, Teruhiko Tanigawa, Kiyotaka Machida, Toshio Tanaka, Makoto Taniguchi

{"title":"Synthesis of uridine 5′-monophosphate glucose as an inhibitor of UDP-glucose pyrophosphorylase","authors":"Ken-Ichi Fujita, Teruhiko Tanigawa, Kiyotaka Machida, Toshio Tanaka, Makoto Taniguchi","doi":"10.1016/S0922-338X(98)80052-4","DOIUrl":"10.1016/S0922-338X(98)80052-4","url":null,"abstract":"<div>Uridine 5′-monophosphate α-d-glucose (UMPG) was evaluated as a novel and potent inhibitor of the enzymatic reaction involved in sugar nucleotide metabolism. UMPG was synthesized by chemical coupling of 2,3,4,6-tetra-O-acetyl-α-d-glucopyranosyl bromide with uridine 5′-monophosphate (UMP) to give uridine 5′-monophosphate 2″,3″,4″,6″-tetra-O-acetyl-α-d-glucose (UMPTAG), followed by deacetylation of UMPTAG with sodium methoxide. In addition to UMPG, UMPTAG showed potent inhibitory activity toward yeast UDPG pyrophosphorylase (UDPG synthetase). UMPG and UMPTAG were competitive with UDPG in the pyrophosphorolytic reaction, with inhibition constants (Ki) of 4.8 and 20.7 μM, respectively, but non-competitive with inorganic pyrophosphate. UMPG and UMPTAG also inhibited the enzyme non-competitively in the reverse reaction to synthesize UDPG from UTP and glucose 1-phosphate (G1P). The acetyl group of UMPTAG was thought to enhance its hydrophobic interaction, possibly with an active site region of the enzyme functional for binding with UDPG.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 2","pages":"Pages 145-148"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80052-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82831198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3