Ethanol production by dark fermentation in the marine green alga, Chlorococcum littorale

Yoshiyuki Ueno , Norihide Kurano , Shigetoh Miyachi
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引用次数: 136

Abstract

Dark fermentation in the marine green alga, Chlorococcum littorale, was investigated with emphasis on ethanol production. Under dark anaerobic conditions, 27% of cellular starch was consumed within 24 h at 25°C, the cellular starch decomposition being accelerated at higher temperatures. Ethanol, acetate, hydrogen and carbon dioxide were obtained as fermentation products. The maximum productivity of ethanol was 450 μmol/g-dry wt. at 30°C. The fermentation pathway for cellular starch was proposed from the yields of the end-products and the determined enzyme activities. Ethanol was formed from pyruvate by pyruvate decarboxylase and alcohol dehydrogenase. the change in fermentation pattern that varied with cell concentration in the reaction vials suggested that the hydrogen partial pressure affected the consumption mode of reducing equivalents under dark fermentation. Ethanol productivity was improved by adding methyl viologen, while hydrogen production decreased.

海洋绿藻褐藻暗发酵生产乙醇的研究
研究了海洋绿藻(chlorcoccum littorale)的暗发酵,重点研究了其乙醇的生产。在暗厌氧条件下,在25℃下,27%的细胞淀粉在24 h内被消耗,在更高的温度下,细胞淀粉的分解加速。发酵产物为乙醇、乙酸酯、氢气和二氧化碳。在30℃条件下,乙醇的最大产率为450 μmol/g-dry wt。从最终产物的产率和测定的酶活性两方面提出了细胞淀粉的发酵途径。丙酮酸脱羧酶和乙醇脱氢酶催化丙酮酸合成乙醇。发酵模式随反应瓶中细胞浓度的变化而变化,说明氢分压影响暗发酵条件下还原性当量的消耗模式。添加甲基紫堇提高了乙醇产率,但降低了氢气产率。
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