Effect of ethanol on bacterial cellulose production from fructose in continuous culture

Takaaki Naritomi, Tohru Kouda, Hisato Yano, Fumihiro Yoshinaga
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引用次数: 91

Abstract

Effect of ethanol on bacterial cellulose (BC) production from fructose by Acetobacter xylinum subsp. sucrofermentans BPR3001A was investigated in continuous culture. Supplementation of 10 g·l−1 ethanol to the feed medium containing 30 g·l−1 fructose as the main carbon source enhanced the ATP content of viable cells, cell concentration, and fructose consumption rate, establishing a 72-h steady state with 0.95 g·l−1·h−1 of BC production rate and 46% of BC yield in continuous culture with a dilution rate of 0.07 h−1. However, an increase in concentration of ethanol to more than 15 g·l−1 decreased BC production rate due to the inhibition of cell growth by the generated acetate. Results from batch culture experiment using ethanol as the main carbon source suggested that ethanol functioned as an energy source for ATP generation, and not as a substrate for BC biosynthesis. On the other hand, fructose hexokinase (FHK) activity was increased and activities of glucose 6-phosphate dehydrogenases (G6PDs) were inhibited by ATP, suggesting that the improved BC production from fructose by ethanol supplementation was a result of increased fructose dissimilation and abundant flow of glucose 6-phosphate (G6P), a precursor of BC, into the BC biosynthetic pathway due to the inhibition of G6PDs by increased levels of ATP.

连续培养中乙醇对细菌从果糖中生产纤维素的影响
乙醇对木醋杆菌从果糖生产细菌纤维素的影响在连续培养中对BPR3001A进行了研究。在含有30 g·l−1果糖的主要碳源培养基中添加10 g·l−1乙醇,可提高活细胞ATP含量、细胞浓度和果糖消耗率,在连续培养中达到72 h稳态,BC产量为0.95 g·l−1·h−1,BC产量为46%,稀释率为0.07 h−1。然而,当乙醇浓度增加到15 g·l−1以上时,由于生成的乙酸抑制了细胞的生长,导致BC的产量降低。以乙醇为主要碳源的分批培养实验结果表明,乙醇作为ATP生成的能量来源,而不是作为BC生物合成的底物。另一方面,果糖己糖激酶(FHK)活性增加,葡萄糖6-磷酸脱氢酶(g6pd)活性受到ATP的抑制,这表明乙醇对果糖生成BC的改善是由于葡萄糖的异化增加,以及葡萄糖的前体葡萄糖6-磷酸(G6P)通过增加ATP水平抑制g6pd而大量流入BC生物合成途径的结果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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