Production and immobilization of lipase from Aeromonas sobria harboring a heterologous gene

Abstract

Aeromonas sobria LP004 harboring the Acinetobacter calcoaceticus lipase gene was designated as strain LP094. Lipase production of LP094 in WYGS medium [Lotrakul and Dharmsthiti, World J. Microbiol. Biotechnol., 13 : 163–166, 1997] was scaled up to a 50-l volume. LP094 lipase immobilized by ionic binding to either IR120 (Na⁺) or IRC50 (H⁺) Amberlite resins was found to retain more than 90% activity after 15 d storage at room temperature (≈ 25 to 30°C) or at 4°C. After 5 repeated lipid hydrolysis reactions, the activities of both immobilized preparations remained higher than 65%.