Journal of Fermentation and Bioengineering最新文献_第4页

Biological activities of abscisic acid analogs in the morphological change of the green alga Haematococcus pluvialis 脱落酸类似物在绿藻雨红球藻形态变化中的生物活性

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80076-7

Makio Kobayashi , Yasushi Todoroki , Nobuhiro Hirai , Yoshiro Kurimura , Hajime Ohigashi , Yasunobu Tsuji

引用次数: 16

Effect of oxygen on the growth of Clostridium butyricum (type species of the genus Clostridium), and the distribution of enzymes for oxygen and for active oxygen species in Clostridia 氧对丁酸梭菌(梭菌属模式种)生长的影响及梭菌体内氧酶和活性氧酶的分布

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)89006-0

Sinji Kawasaki , Tomoyuki Nakagawa , Yoshitaka Nishiyama , Yoshimi Benno , Tai Uchimura , Kazuo Komagata , Michio Kozaki , Youichi Niimura

{"title":"Effect of oxygen on the growth of Clostridium butyricum (type species of the genus Clostridium), and the distribution of enzymes for oxygen and for active oxygen species in Clostridia","authors":"Sinji Kawasaki , Tomoyuki Nakagawa , Yoshitaka Nishiyama , Yoshimi Benno , Tai Uchimura , Kazuo Komagata , Michio Kozaki , Youichi Niimura","doi":"10.1016/S0922-338X(99)89006-0","DOIUrl":"10.1016/S0922-338X(99)89006-0","url":null,"abstract":"<div>Clostridia are well-known obligatory anaerobic bacteria which cannot utilize oxygen, or otherwise die in oxygenated environments. Clostridium butyricum, the type species of the genus Clostridium, possesses the ability to consume oxygen in amounts proportional to the size of the inoculum. Oxygen consumption was observed when NADH and NADPH were added to the cell extract of this strain. NADH oxidase and NADPH oxidase activities were also detected in all of the tested strains of the genus Clostridium. C. butyricum ceased growing while consuming oxygen in the medium. After consumption of all the dissolved oxygen, C. butyricum resumed growth at a rate equivalent to its anaerobic growth rate, suggesting that no oxidative damage based on oxygen reduction occurs in vivo. As scavengers for active oxygen species, the activities of <math><mtext>NADH</mtext><mtext>NADPH</mtext></math> peroxidase and SOD were detected in C. butyricum. Furthermore, the activities of these enzymes are distributed widely in the genus Clostridium.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89006-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74888187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis 搅拌陶瓷膜反应器过滤性能的改进及其在乳酸乳球菌密集细胞培养快速发酵乳酸中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80087-1

Yuya Kamoshita, Ryo Ohashi, Takahiro Suzuki

{"title":"Improvement of filtration performance of stirred ceramic membrane reactor and its application to rapid fermentation of lactic acid by dense cell culture of Lactococcus lactis","authors":"Yuya Kamoshita, Ryo Ohashi, Takahiro Suzuki","doi":"10.1016/S0922-338X(98)80087-1","DOIUrl":"10.1016/S0922-338X(98)80087-1","url":null,"abstract":"<div>A ceramic filter was fitted in a stirred ceramic membrane reactor (SCMR) for both extraction of culture supernatant and feeding of distilled water in reverse flow. The dependence of filtration performance on the cell concentration was decreased by about 20% by regularly cleaning the filter using a membrane cleaning system. The improved permeability effected an increase of both the growth rate and viability of Lactococcus lactis by increasing the dilution rate of the culture supernatant. Using the improved SCMR system, a cell concentration of 178 g/l and viability of 98% were obtained after 198 h of culture, while it took 238 h to obtain a cell concentration of 141 g/l and 94% viability without the use of the membrane cleaning system. The perfusion culture system was applied to the rapid batch fermentation of lactic acid by retaining cells at a high density in the SCMR. When the cell concentration reached 80 g/l, the culture supernatant was extracted and replaced with the fermentation medium. Batch fermentation using the retained cells was repeated six times. The concentration of lactic acid increased to more than 30 g/l within 2 h in each fermentation, while 1.2 h was necessary for replacing the culture supernatant to repeat the batch fermentation. The production rate of lactic acid was increased in proportion to the cell concentration, and a high fermentation activity of the retained cells was maintained via the repeated batch fermentation. These results demonstrate that the improved permeability of the SCMR with use of a membrane cleaning system effected a rapid increase in the concentration and viability of cells, and accordingly, the increased production rate of lactic acid in proportion to the concentration of viable cells.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80087-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75956811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 28

Alanine racemase gene fragments as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods 丙氨酸消旋酶基因片段在食品中嗜脂嗜热芽孢杆菌和嗜冷糖芽孢杆菌检测中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80005-6

Yoko Okubo, Kumio Yokoigawa, Hiroyasu Kawai

{"title":"Alanine racemase gene fragments as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods","authors":"Yoko Okubo, Kumio Yokoigawa, Hiroyasu Kawai","doi":"10.1016/S0922-338X(98)80005-6","DOIUrl":"10.1016/S0922-338X(98)80005-6","url":null,"abstract":"<div>Alanine racemase gene fragments containing non-conserved regions of the gene were evaluated as probes for detecting Bacillus stearothermophilus and Bacillus psychrosaccharolyticus in foods. The gene fragments were amplified from genomic DNA of each bacterium by polymerase chain reaction with degenerate oligonucleotide primers, and labeled with digoxigenin as probes for detecting each bacterium. Foods and bacteria were treated at 25°C for 10 min in 0.1 N NaOH containing 0.5% SDS before being directly spotted onto nylon membranes for DNA hybridization. When the specificities of the probes were analyzed using a total of 86 strains (23 genera and 65 species) of bacteria including 29 Bacillus strains (20 species), each probe was specific for the respective target bacteria. A variety of foods inoculated with B. stearothermophilus or B. psychrosaccharolyticus were positive as determined by hybridization with the respective probe, whereas uninoculated foods were negative. The alanine racemase gene fragments could be used as specific probes for detecting B. stearothermophilus and B. psychrosaccharolyticus in foods.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80005-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75789087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Succinoglycan production by Agrobacterium tumefaciens 农杆菌产琥珀聚糖的研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80083-4

Miroslav Stredansky , Elena Conti , Claudia Bertocchi , Maria Matulova , Flavio Zanetti

引用次数: 25

Discovery of a new mechanism of 2,3-butanediol stereoisomer formation in Bacillus cereus YUF-4 蜡样芽孢杆菌YUF-4中2,3-丁二醇立体异构体形成新机制的发现

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80358-3

S. Ui, T. Hosaka, Kazuhide Watanabe, A. Mimura

引用次数: 21

Lipase-catalyzed kinetic resolution of 4-hydroxy-5-dodecynonitrile and its application to facile synthesis of a cupreous chafer beetle sex pheromone 脂肪酶催化4-羟基-5-十二烷基腈的动力学分解及其在铜金龟子性信息素合成中的应用

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80161-X

Ei-Ichiro Fukusaki , Shiro Satoda , Shuji Senda , Tetsuo Omata

引用次数: 3

Supplementation of NaCl to starter culture of the soy yeast Zygosaccharomyces rouxii 添加NaCl对大豆酵母鲁氏酵母发酵剂培养的影响

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80021-4

Makio Kobayashi, Satomi Hayashi

引用次数: 12

Development of chitosan-conjugated magnetite for magnetic cell separation 壳聚糖偶联磁铁矿的研制

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80060-3

Hiroyuki Honda, Atsushi Kawabe, Masashige Shinkai, Takeshi Kobayashi

{"title":"Development of chitosan-conjugated magnetite for magnetic cell separation","authors":"Hiroyuki Honda, Atsushi Kawabe, Masashige Shinkai, Takeshi Kobayashi","doi":"10.1016/S0922-338X(98)80060-3","DOIUrl":"10.1016/S0922-338X(98)80060-3","url":null,"abstract":"<div>For the purpose of separating microorganisms from culture broth by magnetic force, magnetic particles were conjugated with a polymer to produce polymer-conjugated magnetite (polymer-mag). Among 4 preparation methods investigated—aminosilane coupling, glycidylsilane coupling, crosslinking, and co-precipitation—, polymer-mag prepared by co-precipitation showed the highest cell recovery and high dispersibility. When various cationic, anionic, and nonionic polymers were used to prepare polymer-mag and applied to the magnetic separation for Escherichia coli, magnetite conjugated with chitosan (chitosan-mag) gave the highest cell recovery. In addition, E. coli cells could be recovered as the precipitant only 1 min after chitosan-mag was added to a cell suspension, and a clarified supernatant was obtained. The amount of E. coli cells adsorbed to the chitosan-mag was about 1 g-dry cells/g-chitosan-mag, and cell recovery of over 90% was attained in wide pH range from 3.0 to 7.0. Of 12 microorganisms tested, 4 could be recovered with chitosan-mag at recovery levels above 90%, and the adsorbed amounts exceeded 0.5 g-dry cells/g-chitosan-mag. Differences in adsorbed amounts were considered to be mainly due to the different zeta potential of the microorganisms tested.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80060-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84452104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 76

Control of cell growth rate by sugar feeding based on CO2 production rate 以CO2产率为基础，通过喂糖控制细胞生长速率

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)85690-5

Mutsumi Takagi, Fumihiro Ishimura, Isao Fujimatsu

{"title":"Control of cell growth rate by sugar feeding based on CO2 production rate","authors":"Mutsumi Takagi, Fumihiro Ishimura, Isao Fujimatsu","doi":"10.1016/S0922-338X(97)85690-5","DOIUrl":"10.1016/S0922-338X(97)85690-5","url":null,"abstract":"<div>Sugar feeding based on the CO2 production rate was investigated for the control of the cell growth rate during cephalosporin C fermentation in which sugar concentration was the growth limiting factor. The rates of cell growth and sugar consumption were apparently influenced by the sugar feed rate, as the sugar concentration in the broth was kept at approximately 1 g/l during the cultivation. The ratio of the CO2 production rate to the sugar consumption rate was maintained almost constant after 40 h cultivation. Stepwise alteration in sugar feed rate after the culture time of 50 h caused a change in the CO2 production rate within 15 min. These results indicated that the CO2 production rate could be used as an effective parameter of sugar consumption and cell growth rates. Consequently, a control strategy was developed that involved the control of sugar feed rate so that a CO2 production rate profile corresponded to a preset standard profile. For the half the usual amount of inoculum, the sugar feeding control system enabled the cell concentration to increase faster to that in the usual inoculum. The cell concentration deviation during exponential growth phase among several batches, decreased to half using this sugar feeding control system compared to the culture without such control. These results indicated that the sugar feeding control system developed in this report was found to be efficient for control of cell growth.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)85690-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79881908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1