Journal of Fermentation and Bioengineering最新文献_第3页

Separation of lactic acid using polymeric membrane containing a mobile carrier 用含移动载体的聚合膜分离乳酸

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80066-4

Michiaki Matsumoto, Toshiyuki Takagi, Kazuo Kondo

引用次数: 38

A novel arylesterase active toward 7-aminocephalosporanic acid from Agrobacterium radiobacter IFO 12607: Nucleotide sequence, gene expression in Escherichia coli, and site-directed mutagenesis 放射农杆菌IFO 12607对7-氨基头孢酸具有活性的新型芳基酯酶:核苷酸序列、基因在大肠杆菌中的表达和位点定向诱变

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)86757-8

Yasuyoshi Sakai , Kaoru Ayukawa , Ryoji Mitsui , Hiroya Yurimoto , Keizo Yamamoto , Nobuo Kato

{"title":"A novel arylesterase active toward 7-aminocephalosporanic acid from Agrobacterium radiobacter IFO 12607: Nucleotide sequence, gene expression in Escherichia coli, and site-directed mutagenesis","authors":"Yasuyoshi Sakai , Kaoru Ayukawa , Ryoji Mitsui , Hiroya Yurimoto , Keizo Yamamoto , Nobuo Kato","doi":"10.1016/S0922-338X(97)86757-8","DOIUrl":"10.1016/S0922-338X(97)86757-8","url":null,"abstract":"<div>A novel arylesterase from Agrobacterium radiobacter IFO 12607 catalyzes the deacetylation of 7-aminocephalosporanic acid (7-ACA) to form deacetyl 7-ACA, but is inactive with cephalosporin C. A DNA fragment carrying the gene encoding the 7-ACA-deacetylating enzyme was cloned from the chromosomal DNA of this bacterium. The open reading frame encoding the enzyme was 642 bp long, corresponding to a protein of 214 amino acid residues (molecular mass=23,085). The deduced amino acid sequence did not contain the sequence GXSXG, typical of the many serine esterases including Bacillus cephalosporin C deacetylase, but has the pentapeptide motif sequence GDSLT (amino acid position 9–13) which is also a consensus sequence of some serine esterases. The newly cloned gene was expressed in Escherichia coli under the control of the lac promoter, and the gene product purified from E. coli exhibited the same catalytic properties as the enzyme purified from A. radiobacter. Site-directed mutagenesis of S11A or S11C within the pentapeptide motif sequence led to complete loss of the enzyme activity. Thus, the Ser-11 residue within the GDSLT motif sequence was determined to construct the catalytic center. These results together with those of our previous studies indicated that the 7-ACA-deacetylating enzyme from A. radiobacter IFO 12607 is a new member of the family of lipolytic serine esterases containing the GDSLT sequence as their catalytic center.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 2","pages":"Pages 138-143"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)86757-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83540261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Importance of five amino acid residues at C-terminal region for the folding and stability of β-glucosidase of Cellvibrio gilvus gilvus Cellvibrio c端5个氨基酸残基对β-葡萄糖苷酶折叠和稳定性的重要性

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80089-5

Jong Deog Kim , Satya Singh , Sachiko Machida , Young Yu , Chika Aoyagi , Yasushi Kawata , Kiyoshi Hayashi

引用次数: 11

Cyclic AMP regulation of the mob operon of the non-self-transmissible plasmid pEC3 isolated from a phytopathogenic bacterium 从植物致病菌中分离的非自传质粒pEC3的环状AMP调控的mob操纵子

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(99)80002-6

Nobuhiko Nomura , Mitsuo Yamashita , Yoshikatsu Murooka

引用次数: 0

Purification and characterization of an endo-1,4-β-d-galactanase from Aspergillus sojae 大豆曲霉内生-1,4-β-d-半乳糖酶的纯化及特性研究

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)80352-2

Isao Kimura , Naomi Yoshioka , Shigeyuki Tajima

引用次数: 17

Characterization of a multidomain cellulase from an extremely thermophilic anaerobe strain NA10 极端嗜热厌氧菌株NA10多结构域纤维素酶的表征

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(97)85677-2

Katsuhide Miyake, Yuichi Machida, Kouji Hattori, Shinji Iijima

{"title":"Characterization of a multidomain cellulase from an extremely thermophilic anaerobe strain NA10","authors":"Katsuhide Miyake, Yuichi Machida, Kouji Hattori, Shinji Iijima","doi":"10.1016/S0922-338X(97)85677-2","DOIUrl":"10.1016/S0922-338X(97)85677-2","url":null,"abstract":"<div>The nucleotide sequence of a β-glucanase gene from an extremely thermophilic anaerobe NA10 was determined. The open reading frame extended over 3000 bp and encoded a polypeptide with a molecular mass of 113 kDa. The deduced amino acid sequence of this protein exhibited high homology to a bifunctional cellulase CelB of Caldocellum saccharolyticum. Based on the homology to CelB, the NA10 β-glucanase appears to comprise three domains: N-terminal, central, and C-terminal domains. Among these, N- and C-terminal domains apper to be catalytic domains, and the central domain to be a cellulose binding domain. These domains were joined with each other by proline and threonine rich segments (PT box). The GST-fused C-terminal domain showed CMCase and MUCase activities, but the activities of the GST-fused N-terminal domain were very weak. Zymogram analysis revealed that recombinant Escherichia coli containing the β-glucanase gene produced a protein with a molecular mass of approximately 113 kDa, which was in good agreement with that deduced from the DNA sequence. However, Western blot analysis indicated that the amount of this full length protein was very small. Several smaller abundant proteins which exhibited CMCase activity were also detected. Northern blot analysis indicated that there appear to be putative internal transcriptional initiation sites. Generation of small molecular mass species appear to be due to alternative transcription and translation from the initiation sites within the gene, or partial proteolysis.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"85 3","pages":"Pages 289-296"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(97)85677-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89635948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Cloning and sequence analysis of fumarase and superoxide dismutase genes from an extreme thermophile Thermus thermophilus HB27 极端嗜热菌HB27富马酸酶和超氧化物歧化酶基因的克隆与序列分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80045-7

Takehide Kosuge, Kaho Umehara, Takayuki Hoshino

引用次数: 3

Key word index 关键词索引

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80127-X

引用次数: 0

Contents to volume 85 第85卷目录

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)90003-4

引用次数: 0

Cloning and nucleotide sequencing of genes encoding Mn-superoxide dismutase and class II fumarase from Thermus aquaticus YT-1 水热菌mn -超氧化物歧化酶和II类富马酸酶基因的克隆与序列分析

Journal of Fermentation and Bioengineering Pub Date : 1998-01-01 DOI: 10.1016/S0922-338X(98)80028-7

Hidemasa Motoshima , Etsuo Minagawa, Fuji Tsukasaki, Shuichi Kaminogawa

{"title":"Cloning and nucleotide sequencing of genes encoding Mn-superoxide dismutase and class II fumarase from Thermus aquaticus YT-1","authors":"Hidemasa Motoshima , Etsuo Minagawa, Fuji Tsukasaki, Shuichi Kaminogawa","doi":"10.1016/S0922-338X(98)80028-7","DOIUrl":"10.1016/S0922-338X(98)80028-7","url":null,"abstract":"<div>A 6955-bp sequence of a PstI-HindIII DNA fragment containing the manganese-superoxide dismutase (MnSOD) gene of Thermus aquaticus YT-1 was determined. The gene (sod) encoded a polypeptide consisting of 204 amino acid residues (the mature enzyme without the initiation methionine) with a calculated Mr of 22,773. The deduced amino acid sequence of the sod gene showed 92% identity to that of Thermus thermophilus HB8 determined chemically. The sod gene was well expressed in Escherichia coli and a heat-stable active enzyme was produced. An open reading frame encoding fumarase was found 91 bp upstream of the MnSOD gene in tandem form. The fum gene encoded a polypeptide consisting of 466 amino acid residues with a calculated Mr of 50,950. The deduced amino acid sequence of the fum gene product has similarity to that of the fumC of E. coli (57% identity), suggesting that this gene encodes a class II type fumarase.</div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 1","pages":"Pages 21-27"},"PeriodicalIF":0.0,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(98)80028-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78550134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6