{"title":"优化Kluyveromyces sp. Y-85高产菊粉酶的培养条件及扩大发酵规模","authors":"Wenling Wei, Zhonghui Zheng, Yueying Liu, Xinsheng Zhu","doi":"10.1016/S0922-338X(99)89011-4","DOIUrl":null,"url":null,"abstract":"<div><p>The response surface method (RSM) was used to optimize the medium for the production of inulinase by <em>Kluyveromyces</em> sp. Y-85. The inulinase production was adequately approximated with a full quadratic equation obtained from a four-factor-five-level central composite design. Analyses of the quadratic surfaces showed that in a 24-h fermentation at 30°C, the maximum inulinase activity 59.5 U/ml appeared at extract of <em>Jerusalem artichoke</em>, urea, beef extract, corn steep liquor concentrations 8.0%, 2.0%, 0.2% and 4.0%, respectively. We further investigated the fermentation in 15 <em>l</em> fermentor and scaling-up in 1000 <em>l</em> tower fermentor. The inulinase activity in the scaling-up was 68.9 U/ml, which was the highest production reported at present, indicating that <em>Kluyveromyces</em> sp. Y-85 was an excellent strain for industrial production.</p></div>","PeriodicalId":15696,"journal":{"name":"Journal of Fermentation and Bioengineering","volume":"86 4","pages":"Pages 395-399"},"PeriodicalIF":0.0000,"publicationDate":"1998-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89011-4","citationCount":"38","resultStr":"{\"title\":\"Optimizing the culture conditions for higher inulinase production by Kluyveromyces sp. Y-85 and scaling-up fermentation\",\"authors\":\"Wenling Wei, Zhonghui Zheng, Yueying Liu, Xinsheng Zhu\",\"doi\":\"10.1016/S0922-338X(99)89011-4\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The response surface method (RSM) was used to optimize the medium for the production of inulinase by <em>Kluyveromyces</em> sp. Y-85. The inulinase production was adequately approximated with a full quadratic equation obtained from a four-factor-five-level central composite design. Analyses of the quadratic surfaces showed that in a 24-h fermentation at 30°C, the maximum inulinase activity 59.5 U/ml appeared at extract of <em>Jerusalem artichoke</em>, urea, beef extract, corn steep liquor concentrations 8.0%, 2.0%, 0.2% and 4.0%, respectively. We further investigated the fermentation in 15 <em>l</em> fermentor and scaling-up in 1000 <em>l</em> tower fermentor. The inulinase activity in the scaling-up was 68.9 U/ml, which was the highest production reported at present, indicating that <em>Kluyveromyces</em> sp. Y-85 was an excellent strain for industrial production.</p></div>\",\"PeriodicalId\":15696,\"journal\":{\"name\":\"Journal of Fermentation and Bioengineering\",\"volume\":\"86 4\",\"pages\":\"Pages 395-399\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S0922-338X(99)89011-4\",\"citationCount\":\"38\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Fermentation and Bioengineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0922338X99890114\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fermentation and Bioengineering","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0922338X99890114","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Optimizing the culture conditions for higher inulinase production by Kluyveromyces sp. Y-85 and scaling-up fermentation
The response surface method (RSM) was used to optimize the medium for the production of inulinase by Kluyveromyces sp. Y-85. The inulinase production was adequately approximated with a full quadratic equation obtained from a four-factor-five-level central composite design. Analyses of the quadratic surfaces showed that in a 24-h fermentation at 30°C, the maximum inulinase activity 59.5 U/ml appeared at extract of Jerusalem artichoke, urea, beef extract, corn steep liquor concentrations 8.0%, 2.0%, 0.2% and 4.0%, respectively. We further investigated the fermentation in 15 l fermentor and scaling-up in 1000 l tower fermentor. The inulinase activity in the scaling-up was 68.9 U/ml, which was the highest production reported at present, indicating that Kluyveromyces sp. Y-85 was an excellent strain for industrial production.