Journal of experimental zoology. Part B, Molecular and developmental evolution最新文献

{"title":"Gene expression differences associated with intrinsic hindfoot muscle loss in the jerboa, Jaculus jaculus","authors":"Mai P. Tran,&nbsp;Daniel Ochoa Reyes,&nbsp;Alexander J. Weitzel,&nbsp;Aditya Saxena,&nbsp;Michael Hiller,&nbsp;Kimberly L. Cooper","doi":"10.1002/jez.b.23268","DOIUrl":"10.1002/jez.b.23268","url":null,"abstract":"<p>Vertebrate animals that run or jump across sparsely vegetated habitats, such as horses and jerboas, have reduced the number of distal limb bones, and many have lost most or all distal limb muscle. We previously showed that nascent muscles are present in the jerboa hindfoot at birth and that these myofibers are rapidly and completely lost soon after by a process that shares features with pathological skeletal muscle atrophy. Here, we apply an intra- and interspecies differential RNA-Seq approach, comparing jerboa and mouse muscles, to identify gene expression differences associated with the initiation and progression of jerboa hindfoot muscle loss. We show evidence for reduced hepatocyte growth factor and fibroblast growth factor signaling and an imbalance in nitric oxide signaling; all are pathways that are necessary for skeletal muscle development and regeneration. We also find evidence for phagosome formation, which hints at how myofibers may be removed by autophagy or by nonprofessional phagocytes without evidence for cell death or immune cell activation. Last, we show significant overlap between genes associated with jerboa hindfoot muscle loss and genes that are differentially expressed in a variety of human muscle pathologies and rodent models of muscle loss disorders. All together, these data provide molecular insight into the process of evolutionary and developmental muscle loss in jerboa hindfeet.</p>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 7","pages":"453-464"},"PeriodicalIF":1.8,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.b.23268","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A microfluidic chip for immobilization and imaging of Ciona intestinalis larvae","authors":"Guillaume Poncelet,&nbsp;Lucia Parolini,&nbsp;Sebastian M. Shimeld","doi":"10.1002/jez.b.23267","DOIUrl":"10.1002/jez.b.23267","url":null,"abstract":"<p>Sea squirts (Tunicata) are chordates and develop a swimming larva with a small and defined number of individually identifiable cells. This offers the prospect of connecting specific stimuli to behavioral output and characterizing the neural activity that links these together. Here, we describe the development of a microfluidic chip that allows live larvae of the sea squirt <i>Ciona intestinalis</i> to be immobilized and recorded. By generating transgenic larvae expressing GCaAMP6m in defined cells, we show that calcium ion levels can be recorded from immobilized larvae, while microfluidic control allows larvae to be exposed to specific waterborne stimuli. We trial this on sea water carrying increased levels of carbon dioxide, providing evidence that larvae can sense this gas.</p>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 7","pages":"443-452"},"PeriodicalIF":1.8,"publicationDate":"2024-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.b.23267","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141283805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic bias in repeated evolution of pigment loss in cave populations of the Asellus aquaticus species complex","authors":"Žiga Fišer,&nbsp;Hana Whitehorn,&nbsp;Tia Furness,&nbsp;Peter Trontelj,&nbsp;Meredith Protas","doi":"10.1002/jez.b.23256","DOIUrl":"10.1002/jez.b.23256","url":null,"abstract":"<p>Similar phenotypes can evolve repeatedly under the same evolutionary pressures. A compelling example is the evolution of pigment loss and eye loss in cave-dwelling animals. While specific genomic regions or genes associated with these phenotypes have been identified in model species, it remains uncertain whether a bias towards particular genetic mechanisms exists. An isopod crustacean, <i>Asellus aquaticus</i>, is an ideal model organism to investigate this phenomenon. It inhabits surface freshwaters throughout Europe but has colonized groundwater on multiple independent occasions and evolved several cave populations with distinct ecomorphology. Previous studies have demonstrated that three different cave populations utilized common genetic regions, potentially the same genes, in the evolution of pigment and eye loss. Expanding on this, we conducted analysis on two additional cave populations, distinct either phylogenetically or biogeographically from those previously examined. We generated F2 hybrids from cave × surface crosses and tested phenotype-genotype associations, as well as conducted complementation tests by crossing individuals from different cave populations. Our findings revealed that pigment loss and orange eye pigment in additional cave populations were associated with the same genomic regions as observed in the three previously tested cave populations. Moreover, the lack of complementation across all cross combinations suggests that the same gene likely drives pigment loss. These results substantiate a genetic bias in the recurrent evolution of pigment loss in this model system. Future investigations should focus on the cause behind this bias, possibly arising from allele recruitment from ancestral surface populations' genetic variation or advantageous allele effects via pleiotropy.</p>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 6","pages":"425-436"},"PeriodicalIF":1.8,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.b.23256","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141198917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In the Spotlight—Established researcher","authors":"Janine M. Ziermann-Canabarro","doi":"10.1002/jez.b.23257","DOIUrl":"10.1002/jez.b.23257","url":null,"abstract":"","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 6","pages":"417-418"},"PeriodicalIF":1.8,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141081680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In the Spotlight: Established researcher","authors":"Virginia Abdala","doi":"10.1002/jez.b.23258","DOIUrl":"10.1002/jez.b.23258","url":null,"abstract":"","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 5","pages":"389-390"},"PeriodicalIF":2.2,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated ammonia cues hatching in red-eyed treefrogs: A mechanism for escape from drying eggs","authors":"Astrid K. Lisondro-Arosemena,&nbsp;María José Salazar-Nicholls,&nbsp;Karen M. Warkentin","doi":"10.1002/jez.b.23253","DOIUrl":"10.1002/jez.b.23253","url":null,"abstract":"<p>Egg dehydration can kill terrestrial frog embryos, and this threat is increasing with climate change and deforestation. In several lineages that independently evolved terrestrial eggs, and retained aquatic tadpoles, embryos accelerate hatching to escape from drying eggs, entering the water earlier and less developed. However, the cues that stimulate drying-induced early hatching are unknown. Ammonia is a toxic, water-soluble metabolic waste that accumulates within eggs as embryos develop and concentrates as eggs dehydrate. Thus, increasing ammonia concentration may be a direct threat to embryos in drying eggs. We hypothesized that it could serve as a cue, stimulating embryos to hatch and escape. The embryos of red-eyed treefrogs, <i>Agalychnis callidryas</i>, hatch early to escape from many threats, including dehydration, and are known to use mechanosensory, hypoxia, and light cues. To test if they also use high ammonia as a cue to hatch, we exposed stage-matched pairs of hatching-competent, well-hydrated sibling embryos to ammonia and control solutions in shallow water baths and recorded their behavior. Control embryos remained unhatched while ammonia-exposed embryos showed a rapid, strong hatching response; 95% hatched, on average in under 15 min. This demonstrates that elevated ammonia can serve as a hatching cue for <i>A. callidryas</i> embryos. This finding is a key step in understanding the mechanisms that enable terrestrial frog embryos to escape from egg drying, opening new possibilities for integrative and comparative studies on this growing threat.</p>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 5","pages":"406-411"},"PeriodicalIF":2.2,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140851108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In the Spotlight—Established researcher","authors":"Ingo Braasch","doi":"10.1002/jez.b.23254","DOIUrl":"https://doi.org/10.1002/jez.b.23254","url":null,"abstract":"&lt;p&gt;&lt;/p&gt;&lt;p&gt;Ingo is a Guest Coeditor of this special issue on &lt;i&gt;Aquatic Models for Biomedical Evo-Devo&lt;/i&gt;.&lt;/p&gt;&lt;p&gt;Website: https://www.fishevodevogeno.org/&lt;/p&gt;&lt;p&gt;Google scholar page: https://scholar.google.com/citations?user=xVw8dCAAAAAJ&lt;/p&gt;&lt;p&gt;I studied biology at the University of Konstanz, Germany, and worked as an undergraduate on my first comparative fish genomics projects in the group of Axel Meyer with two fantastic postdocs at the time: John S. Taylor, now faculty at the University of Victoria, Canada, and Walter Salzburger, now faculty at the University of Basel, Switzerland. For my doctoral work, I joined Manfred Schartl and Jean-Nicolas Volff at the University of Würzburg, also in Germany, studying the functional genetic impacts of whole genome duplications on the evolution of vertebrate pigmentation. For my postdoc, I worked in the group of John H. Postlethwait at the University of Oregon in Eugene. John's group had just started to use spotted gar as a genomic outgroup to the teleost fishes and the teleost genome duplication. There, I began developing spotted gar as a developmental and functional genomic model system for vertebrate biology and EvoDevo – work that continues in my laboratory at Michigan State University.&lt;/p&gt;&lt;p&gt;I grew up in provincial Germany as the son of a high school chemistry and physics teacher and a pharmaceutical technician, so I was exposed to the natural sciences early on. Starting in elementary school, I developed a passion for reading about dinosaurs and prehistoric people, years before &lt;i&gt;Jurassic Park&lt;/i&gt; made paleontology cool. Thus, although I didn't know the term then, I had an early appreciation for macroevolution. In high school, I kept all kinds of aquarium fishes (can you ever have too many tanks?), while reading about Darwin's &lt;i&gt;Voyage of the Beagle&lt;/i&gt;, evolution, and genetics. This fascination kept going and was a reason I chose the University of Konstanz for undergraduate studies because of its strong curricular focus on molecular biology. Working as an undergraduate researcher in the Meyer Lab and being surrounded by an international crew of world-class molecular evolutionary biologists around me – who even used fish models to answer big questions about the deep evolutionary history of vertebrates – was immensely thrilling. Comparing sequences from diverse organisms and reconstructing their evolutionary change across phylogenies, I could practically look back in time! I knew I had found my path. However, sequencing DNA and analyzing genetic information on the computer was not enough for me. Fondly remembering my childhood fish breeding projects and the beauty of watching fish embryos grow, I successively added developmental biology to my research portfolio. The name of my research group, the &lt;i&gt;Fish Evo Devo Geno Lab&lt;/i&gt;, reflects this multipronged approach.&lt;/p&gt;&lt;p&gt;Observing the elegance of developmental processes in many different fish species is my happy place. How could anyone ever just want to ","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 3","pages":"121-122"},"PeriodicalIF":2.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.b.23254","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140814166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trawling aquatic life for new models in biomedical research and evolutionary developmental biology","authors":"Patricia N. Schneider,&nbsp;Frauke Seemann,&nbsp;Matthew P. Harris,&nbsp;Ingo Braasch","doi":"10.1002/jez.b.23255","DOIUrl":"https://doi.org/10.1002/jez.b.23255","url":null,"abstract":"","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 3","pages":"123-125"},"PeriodicalIF":2.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.b.23255","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140814167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"5th Argentinean Meeting on Evolutionary Biology (RABE V): Report on the “Evo-Devo” Extended Symposium","authors":"Eduardo E. Zattara,&nbsp;Marina M. Strelin","doi":"10.1002/jez.b.23252","DOIUrl":"10.1002/jez.b.23252","url":null,"abstract":"<p>Evolutionary developmental biology (Evo-Devo) is flourishing in Latin America, particularly Argentina, where researchers are leveraging this integrative field to unlock the secrets of the region's remarkable biodiversity. A recent symposium held at the 5th Argentinean Meeting on Evolutionary Biology (RABE V) showcased a vibrant Evo-Devo community and the diversity of its research endeavors. The symposium included 3 plenary talks, 3 short talks, and 12 posters, and spanned a range of organisms and approaches. Interestingly, the symposium highlighted a prevalence of “top-down” Evo-Devo studies in the region, where researchers first analyze existing diversity and then propose potential developmental mechanisms. This approach, driven in part by financial constraints and the region's historical focus on natural history, presents a unique opportunity to bridge disciplines like comparative biology, paleontology, and botany. The symposium's success underscores the vital role of Evo-Devo in Latin America, not only for advancing our understanding of evolution but also for providing valuable tools to conserve and manage the region's irreplaceable biodiversity. As Evo-Devo continues to grow in Latin America, fostering collaboration and knowledge exchange within the region and beyond will be crucial for realizing the full potential of this transformative field.</p>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 4","pages":"335-341"},"PeriodicalIF":2.2,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140842332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The domestication of the larynx: The neural crest connection","authors":"Raffaela Lesch,&nbsp;W. Tecumseh Fitch","doi":"10.1002/jez.b.23251","DOIUrl":"10.1002/jez.b.23251","url":null,"abstract":"<p>Wolves howl and dogs bark, both are able to produce variants of either vocalization, but we see a distinct difference in usage between wild and domesticate. Other domesticates also show distinct changes to their vocal output: domestic cats retain meows, a distinctly subadult trait in wildcats. Such differences in acoustic output are well-known, but the causal mechanisms remain little-studied. Potential links between domestication and vocal output are intriguing for multiple reasons, and offer a unique opportunity to explore a prominent hypothesis in domestication research: the neural crest/domestication syndrome hypothesis. This hypothesis suggests that in the early stages of domestication, selection for tame individuals decreased neural crest cell (NCCs) proliferation and migration, which led to a downregulation of the sympathetic arousal system, and hence reduced fear and reactive aggression. NCCs are a transitory stem cell population crucial during embryonic development that tie to diverse tissue types and organ systems. One of these neural-crest derived systems is the larynx, the main vocal source in mammals. We argue that this connection between NCCs and the larynx provides a powerful test of the predictions of the neural crest/domestication syndrome hypothesis, discriminating its predictions from those of other current hypotheses concerning domestication.</p>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"342 4","pages":"342-349"},"PeriodicalIF":2.2,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jez.b.23251","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140581138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}