Supernumerary Chromosomes Enhance Karyotypic Diversification of Narrow-Headed Voles of the Subgenus Stenocranius (Rodentia, Mammalia).

IF 1.8 3区 生物学 Q3 DEVELOPMENTAL BIOLOGY
Svetlana V Pavlova, Svetlana A Romanenko, Sergey N Matveevsky, Aleksander N Kuksin, Ivan A Dvoyashov, Yulia M Kovalskaya, Anastasiya A Proskuryakova, Natalia A Serdyukova, Tatyana V Petrova
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引用次数: 0

Abstract

The subgenus Stenocranius contains two cryptic species: Lasiopodomys gregalis (subdivided into three allopatrically distributed and genetically well-isolated lineages A, B, and C) and Lasiopodomys raddei. To identify karyotype characteristics of this poorly studied cryptic species complex, we used comparative cytogenetic analysis of 138 individuals from 41 localities in South Siberia and Mongolia. A detailed description of the L. raddei karyotype and of the L. gregalis lineage С karyotype is presented for the first time. The A chromosome complement of all examined narrow-headed voles consisted of 2n = 36 and a fundamental number of autosomal arms (FNa) of 50. Between species, patterns of differential staining were similar, though additional C-heterochromatic blocks were found in L. gregalis lineages; Ag-positive nucleolar organizers and ribosomal DNA (rDNA) clusters are located on eight and nine acrocentric pairs, respectively. No B chromosomes (Bs) were found in the Early Pleistocene relic L. raddei, while one to five small heterochromatic acrocentric Bs were detected in all L. gregalis lineages; the number and frequency of Bs varied considerably within lineages, but no intraindividual variation was observed. In both species, telomeric repeats were visualized at termini of all chromosomes, including Bs. The number and localization of rDNA clusters on Bs varied among B-carriers. Immunodetection of several meiotic proteins indicated that meio-Bs are transcriptionally inactive and have a pattern of meiotic behavior similar to that of sex chromosomes (some homology of Bs to sex chromosomes is supposed). The nature, mechanisms of inheritance and stability of Bs in L. gregalis require further investigation.

超常染色体增强了 Stenocranius 亚属(啮齿目,哺乳纲)窄头田鼠的核型多样性。
Stenocranius 亚属包含两个隐居物种:Lasiopodomys gregalis(细分为三个同域分布且基因分离良好的品系 A、B 和 C)和 Lasiopodomys raddei。为了确定这一研究较少的隐蔽物种群的核型特征,我们对来自南西伯利亚和蒙古 41 个地点的 138 个个体进行了比较细胞遗传学分析。我们首次详细描述了 L. raddei 的核型和 L. gregalis С 系的核型。所有受检窄头田鼠的 A 染色体互补体均为 2n = 36,常染色体臂的基本数目(FNa)为 50。不同物种之间的染色差异模式相似,但在 L. gregalis 品系中发现了额外的 C-异染色质块;Ag 阳性的核小体组织者和核糖体 DNA(rDNA)簇分别位于 8 对和 9 对同心圆上。在早更新世遗民 L. raddei 中没有发现 B 染色体(Bs),而在所有 L. gregalis 系中都检测到了 1 至 5 条小的异染色质非中心 Bs;Bs 的数量和频率在系内差异很大,但没有观察到个体内的差异。在这两个物种中,所有染色体(包括 Bs)的末端都能看到端粒重复序列。Bs 上 rDNA 簇的数量和定位在 B 携带者之间存在差异。对几种减数分裂蛋白的免疫检测表明,meio-Bs 的转录不活跃,其减数分裂行为模式与性染色体类似(Bs 与性染色体应该有一定的同源性)。藻胆中 Bs 的性质、遗传机制和稳定性需要进一步研究。
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来源期刊
CiteScore
4.80
自引率
9.10%
发文量
63
审稿时长
6-12 weeks
期刊介绍: Developmental Evolution is a branch of evolutionary biology that integrates evidence and concepts from developmental biology, phylogenetics, comparative morphology, evolutionary genetics and increasingly also genomics, systems biology as well as synthetic biology to gain an understanding of the structure and evolution of organisms. The Journal of Experimental Zoology -B: Molecular and Developmental Evolution provides a forum where these fields are invited to bring together their insights to further a synthetic understanding of evolution from the molecular through the organismic level. Contributions from all these branches of science are welcome to JEZB. We particularly encourage submissions that apply the tools of genomics, as well as systems and synthetic biology to developmental evolution. At this time the impact of these emerging fields on developmental evolution has not been explored to its fullest extent and for this reason we are eager to foster the relationship of systems and synthetic biology with devo evo.
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