Journal of experimental zoology. Part B, Molecular and developmental evolution最新文献_第6页

Escaping Constraints to Innovate: Maternal Neofunctionalization in a HoxB4 Duplicate 逃避约束以创新：HoxB4副本中母体的新功能化。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2026-01-12 DOI: 10.1002/jezb.70012

Júlia de Lima Carvalho, José Caetano Silva-Filho, Janaina Lima de Oliveira

{"title":"Escaping Constraints to Innovate: Maternal Neofunctionalization in a HoxB4 Duplicate","authors":"Júlia de Lima Carvalho, José Caetano Silva-Filho, Janaina Lima de Oliveira","doi":"10.1002/jezb.70012","DOIUrl":"10.1002/jezb.70012","url":null,"abstract":"Transcription factors are typically thought to play a limited role in developmental evolution due to their high pleiotropic nature. However, such constraints may be relaxed following gene duplication or when proteins are organized into structural and functional modules, opening avenues for evolutionary innovation. Here, we integrate expression and genomic data to investigate the evolutionary dynamics of Hox gene duplicates in the allotetraploid frog Xenopus laevis. Despite overall conservation across the Hox clusters, we find that HoxB4L has acquired expression during maternally regulated stages and is evolving under positive selection. Protein-level changes include the number, length, and sequence of functionally important protein regions. Our results indicate that HoxB4L has escaped ancestral constraints and is undergoing maternal neofunctionalization as a result of cis-regulatory divergence and structural protein modifications. These findings illustrate how transcription factors can overcome developmental constraints and contribute to novel functions during early development.","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"346 2","pages":"141-151"},"PeriodicalIF":1.7,"publicationDate":"2026-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jezb.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145959519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reduced Dietary Protein Induces Changes in the Dental Proteome 饮食蛋白质减少导致牙齿蛋白质组的变化。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2026-01-08 DOI: 10.1002/jezb.70004

Robert W. Burroughs, Christopher J. Percival, Natasha S. Vitek

{"title":"Reduced Dietary Protein Induces Changes in the Dental Proteome","authors":"Robert W. Burroughs, Christopher J. Percival, Natasha S. Vitek","doi":"10.1002/jezb.70004","DOIUrl":"10.1002/jezb.70004","url":null,"abstract":"Experimental studies have demonstrated that nutritional changes during development can result in phenotypic changes to mammalian cheek teeth. This developmental plasticity of tooth morphology is an example of phenotypic plasticity. Because tooth development occurs through complex interactions between manifold processes, there are many potential mechanisms which can contribute to a tooth's norm of reaction. Determining the identity of those mechanisms and the relative importance of each of them is one of the main challenges to understanding phenotypic plasticity. Quantitative proteomics combined with experimental studies allow for the identification of potential molecular contributors to a plastic response through quantification of expressed gene products. Here, we present the results of a quantitative proteomics analysis of mature upper first molars in Mus musculus from a controlled feeding experiment. Pregnant and nursing mothers were fed either a low-dietary protein (10%) treatment diet or control (20%) diet. Low-dietary protein was not associated with reduced molar size or skull length. However, expression of tooth-related proteins, immune system proteins, and actin-based myosin proteins were significantly altered in our low-dietary protein proteomics sample. The differential expression of immune proteins along with systematic reduction in actin-based myosin protein expression are novel discoveries for tooth proteomics studies. We propose that studies that aim to elucidate specific mechanisms of molar phenotypic plasticity should prioritize investigations into the relationships between IGF regulation and tooth development and actin-based myosin expression and tooth development.","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"346 2","pages":"107-127"},"PeriodicalIF":1.7,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jezb.70004","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Heterochrony and Evolution of Oral Structures in Phyllopharyngean Ciliates (Protista: Ciliophora) phyllophyngean纤毛虫口腔结构的异时性及其进化（原生目：纤毛虫目）

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-12-30 DOI: 10.1002/jezb.70009

Klára Bukovinská, Peter Vďačný

{"title":"On the Heterochrony and Evolution of Oral Structures in Phyllopharyngean Ciliates (Protista: Ciliophora)","authors":"Klára Bukovinská, Peter Vďačný","doi":"10.1002/jezb.70009","DOIUrl":"10.1002/jezb.70009","url":null,"abstract":"<div>\u0000 \u0000 Heterochronic shifts in developmental sequences of unicellular organisms are rarely considered as mechanisms behind their morphological evolution. Among eukaryotic microbes, ciliates represent a key model group, exhibiting diverse morphologies and developmental trajectories. To explore the morphological evolution of oral structures in connection with modifications in the relative timing of developmental events, we focused on the orally diverse ciliate class Phyllopharyngea. Two of its subclasses (Rhynchodia and Cyrtophoria) follow the “standard” ontogenetic sequence (stomatogenesis–karyokinesis–cytokinesis), while two subclasses (Suctoria and Chonotrichia) have convergently deviated from the ancestral mode regarding the timing of stomatogenesis. Daughter cells of suctorians and chonotrichs bud from their mother cells, causing juveniles (so-called swarmers) to differ strikingly from adults. The transformation of free-swimming swarmers into sessile adults represents a newly inserted step in the ontogeny of suctorians and chonotrichs. The elongated juvenile phase enabled further extrapolation beyond the terminal morphology of their ancestors (i.e., peramorphosis), allowing a transition from a mobile to a sessile lifestyle in the adult stage. On the other hand, the interrupted and/or postponed stomatogenesis is a neotenic shift (i.e., paedomorphosis). Thus, the adult morphologies of suctorians and chonotrichs are the result of mosaic heterochrony, in which various traits are affected by different types of heterochronic processes. Modifications in the rank order of developmental events and the relative elongation of the ontogenetic timeline are correlated with shifts in the feeding strategies of suctorians and chonotrichs. This review also compiles current knowledge about the morphological evolution of oral structures in the four phyllopharyngean subclasses.</div>","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"346 2","pages":"91-106"},"PeriodicalIF":1.7,"publicationDate":"2025-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shell Proteome Plasticity Assists Oyster Larval Biomineralization in Adverse Carbonate Chemistry 壳蛋白质组可塑性有助于牡蛎幼虫在不利的碳酸盐化学中生物矿化。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-12-07 DOI: 10.1002/jezb.70003

Alessia Carini, Juan Diego Gaitán-Espitia, Vengatesen Thiyagarajan

{"title":"Shell Proteome Plasticity Assists Oyster Larval Biomineralization in Adverse Carbonate Chemistry","authors":"Alessia Carini, Juan Diego Gaitán-Espitia, Vengatesen Thiyagarajan","doi":"10.1002/jezb.70003","DOIUrl":"10.1002/jezb.70003","url":null,"abstract":"Bivalve planktonic development is a critical phase during which larvae must secrete the first calcium carbonate shell, the prodissoconch I (PD I). As PD I formation is in close contact with seawater, this process can be negatively affected by adverse seawater carbonate chemistry. It is hypothesized that bivalves can regulate shell formation under environmental stress through biologically controlled biomineralization involving a complex extracellular shell proteome. However, the plasticity of this regulatory mechanism during PD I development is unknown. We assessed the PD I shell proteome of the Hong Kong oyster (Magallana hongkongensis) in carbonate chemistry that was adverse or favorable for biomineralization to understand the regulatory capacity of larval shell formation. While survival rates were not affected in adverse carbonate chemistry, there were significant changes, including the upregulation of several calcium-binding proteins and downregulation of proton-generating processes and putative calcification inhibitors. With 198 sequences, the oyster larval shell proteome was twice to over six times larger than those reported for other bivalve species at the same developmental stage. However, in adverse carbonate chemistry, the oyster larval shells were thinner and smaller, and protein diversity decreased to 131 sequences, with overall lower functional redundancy and reduced expression of structural proteins, indicating potential trade-offs. The proteomic and shell structural data also suggest that direct cellular control and biologically induced mechanisms, which will require further investigation, may be involved in PD I formation.","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"346 1","pages":"67-83"},"PeriodicalIF":1.7,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Annelid Perspectives Into the Role of FGF Signaling in Caudal Regeneration 从环节动物角度探讨FGF信号在尾侧再生中的作用。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-12-07 DOI: 10.1002/jezb.70008

Alexandra Y. Shalaeva, Vitaly V. Kozin

{"title":"Annelid Perspectives Into the Role of FGF Signaling in Caudal Regeneration","authors":"Alexandra Y. Shalaeva, Vitaly V. Kozin","doi":"10.1002/jezb.70008","DOIUrl":"10.1002/jezb.70008","url":null,"abstract":"Fibroblast growth factor signaling plays a crucial role in various developmental processes and is a key driver of regeneration. In annelids, this pathway is active from the earliest stages of reparative morphogenesis, yet its specific function remains unclear. Here we have functionally examined FGF signaling following the amputation of posterior segments in the marine annelid Alitta virens. We utilized the pharmacological agent SU5402 to inhibit the FGF receptor kinase at different time points. With whole-mount in situ hybridization, we analyzed the expression of regulatory genes that pattern posterior territories (cdx, evx, post2), multipotent/germ cells (vasa, piwi), mesodermal tissues (twist), and segmental boundaries (engrailed). Our findings reveal that FGF signaling is essential for blastema induction by promoting dedifferentiation and proliferation of cells at the wound site, but is not involved in posteriorization. On the contrary, this pathway is crucial for differentiation in the proximal (anterior) part of the regenerative bud, impacting its mesodermal derivatives and segment boundary formation. Comparative analysis suggests that while certain functions of FGF signaling, particularly in mesodermal patterning, are conserved across taxa, its role in posterior axis elongation appears to have evolved specifically within the vertebrate lineage. This study enhances our understanding of the evolutionary origins and functional diversification of FGF signaling in regeneration, positioning A. virens as a valuable model for exploring the complexities of regenerative biology.","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"346 1","pages":"34-47"},"PeriodicalIF":1.7,"publicationDate":"2025-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145701071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-12-01

引用次数: 0

Growth, Remodeling, and Proliferation in the Phoronid Phoronopsis harmeri: The Posterior, the Regionalized, and the Scattered 栉水母的生长、重塑和增殖：后缘、区域化和分散。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-12-01 DOI: 10.1002/jezb.70001

Evgeny G. Ivashkin, Olga I. Taimanova, Anton I. Bogomolov, Elena N. Temereva

{"title":"Growth, Remodeling, and Proliferation in the Phoronid Phoronopsis harmeri: The Posterior, the Regionalized, and the Scattered","authors":"Evgeny G. Ivashkin, Olga I. Taimanova, Anton I. Bogomolov, Elena N. Temereva","doi":"10.1002/jezb.70001","DOIUrl":"10.1002/jezb.70001","url":null,"abstract":"Cell proliferation is a key driver of morphogenesis and body plan transformation in multicellular animals, yet its spatial organization remains poorly understood in many non-segmented spiralians. In this study, we examine the dynamics of cell division during larval growth and metamorphosis in the larvae and early juveniles of the phoronid Phoronopsis harmeri, using EdU incorporation, anti-phospho-histone H3 immunostaining, confocal laser scanning microscopy, and electron microscopy. Early larval proliferation is partly regionalized from the outset and becomes progressively more localized toward metamorphosis. We identify a tripartite organization of proliferative activity: (1) posterior ring-shaped domains in the telotroch that persist through metamorphosis and support elongation and anal chamber formation; (2) regional proliferative zones at tentacle bases, preoral and postoral regions; and (3) scattered proliferation driving the expansion of the trunk epidermis. This coexistence of posterior, regional, and scattered patterns underscores the developmental plasticity of phoronids and the diversity of growth strategies within Spiralia. Posterior proliferative domains in phoronids contribute important context to homology–convergence debates on posterior growth across spiralians, but are not decisive by themselves; viewed with the distributed epithelial proliferation, they underscore the coexistence of multiple proliferative programs within a single life cycle. In addition, we identify atypical mitotic characteristics in this species, including unconventional metaphase organization and signs of interkinetic nuclear migration in larval epithelia. Our results suggest that phoronids provide a valuable model for exploring how diverse architectures of cell proliferation contribute to larval growth, body elongation, and morphogenetic compartmentalization in Lophotrochozoa.","PeriodicalId":15682,"journal":{"name":"Journal of experimental zoology. Part B, Molecular and developmental evolution","volume":"346 1","pages":"48-66"},"PeriodicalIF":1.7,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In the Spotlight—Established Researcher 聚光灯下的知名研究人员。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-11-29 DOI: 10.1002/jezb.70000

H. Frederik Nijhout

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In the Spotlight—Established Researcher 聚光灯下的知名研究人员。

IF 1.7 3区生物学

Journal of experimental zoology. Part B, Molecular and developmental evolution Pub Date : 2025-11-25 DOI: 10.1002/jezb.70002

Mariana Benítez

引用次数: 0

IF 1.7 3区生物学