Journal of Diabetes Research最新文献

{"title":"Delving Into the Interaction Between Exercise and Diabetes on Methylation of the FKBP5 Gene.","authors":"Teng-Chi Yang, Jen Pi Tsai, Honda Hsu, Yen-Chung Chen, Yi-Chia Liaw, Shu Yi Hsu, Hao Jan Yang, Yung-Po Liaw","doi":"10.1155/jdr/1162708","DOIUrl":"10.1155/jdr/1162708","url":null,"abstract":"<p><p><b>Objective:</b> FKBP5 is a critical gene involved in regulating the hypothalamic-pituitary-adrenal (HPA) axis and stress response. Aberrant DNA methylation at FKBP5 cytosine-phosphate-guanine (CpG) sites, such as cg22363520 and cg00862770, has been implicated in mental health disorders and metabolic diseases, including Type 2 diabetes. Exercise is a modulator of DNA methylation and metabolic health. This study investigates the interaction between exercise, diabetes, and FKBP5 methylation at cg22363520 and cg00862770 and explores their implications for mental health and disease development. <b>Materials and Methods:</b> FKBP5 methylation levels at cg22363520 and cg00862770 were analyzed in a cohort stratified by diabetes and exercise. Multiple linear regression models assessed the main effects and interactions of exercise and diabetes on FKBP5 methylation, with further stratified analyses for site-specific effects. <b>Results:</b> Exercise and diabetes showed significant and site-specific effects on FKBP5 methylation at cg22363520 and cg00862770. At cg22363520, exercise significantly reduced methylation levels in nondiabetic participants (<i>β</i> = -0.00195, <i>p</i> = 0.0157), while no significant effect was observed in diabetic individuals. Conversely, at cg00862770, exercise significantly decreased methylation levels in diabetic participants (<i>β</i> = -0.00611, <i>p</i> = 0.0081), with no significant effect in the nondiabetic group. Diabetes itself was associated with increased FKBP5 methylation at both sites, particularly in individuals without regular exercise. Additionally, significant interaction effects between exercise and diabetes were identified for both cg22363520 (<i>p</i> = 0.0336) and cg00862770 (<i>p</i> = 0.0021), highlighting the interplay between metabolic status and physical activity in regulating FKBP5 methylation. <b>Conclusion:</b> This study demonstrates that the effects of exercise on FKBP5 methylation are site-specific and influenced by diabetes status. Exercise reduces methylation at cg22363520 in nondiabetics and at cg00862770 in diabetics, indicating its role in modulating epigenetic regulation of stress and metabolic pathways. These findings underscore the interplay between exercise, diabetes, and FKBP5 methylation, with potential implications for improving mental health and metabolic outcomes.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"1162708"},"PeriodicalIF":3.6,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Value of Glycemic Gap for Predicting Mortality in ICU in Patients With and Without Diabetes.","authors":"Ran Lou, Li Jiang, Meiping Wang, Tingting Wang, Quan Si, Weixue Su, Nan Wang, Yuyan Liu, Ting Chen, Qi Jiang, Bo Zhu","doi":"10.1155/jdr/4563928","DOIUrl":"10.1155/jdr/4563928","url":null,"abstract":"<p><p><b>Objective:</b> Dysglycemia is associated with poor outcomes; the actual status of dysglycemia of critically ill patients with diabetes should refer to background glycemia. We investigated the effect of difference between mean blood glucose and basic blood glucose upon outcomes. <b>Methods:</b> Glycated hemoglobin A1c (HbA1c) was detected within the first 24 h and converted to A1C-derived average glucose (ADAG) by the equation ADAG = [(HbA1c∗28.7) - 46.7]∗18^-1; blood glucose measurements were fourth per day during the first 7 days after admission; the mean blood glucose level (Mean), standard deviation (SD), and coefficient of variation (CV) were calculated. GAP were calculated as admission blood glucose and Mean minus ADAG, respectively. <b>Results:</b> Six hundred forty-nine patients were recruited and 428 survived at 28 days; 302 patients with diabetes had greater ADAG, blood glucose at admission (BG_adm), Mean, SD, CV, GAP, and hypoglycemia incidences. The GAP between Mean and ADAG had superior predictive power, which was decreased in patients with diabetes and increased in patients without diabetes. GAP7 was related to 28-day mortality; the death risk was decreased in patients with diabetes. Patients with lower GAP tended to survive. Nonsurvivors with diabetes suffered higher rate of hypoglycemia than survivors which was the opposite in patients without diabetes. <b>Conclusion:</b> The glycemic GAP between the mean level of blood glucose within the first 7 days in ICU and ADAG was independently associated with 28-day mortality of critically ill patients, which was different between patients with and without diabetes. Hypoglycemia in patients with diabetes should be a concern.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"4563928"},"PeriodicalIF":3.6,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11845263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Should I Take Prediabetes Seriously or Not: A Qualitative Study on People's Perceptions of Prediabetes.","authors":"Katri Harcke, Marit Graue, Timothy Charles Skinner, Christina B Olsson, Dan Grabowski, Nouha Saleh-Stattin","doi":"10.1155/jdr/8063481","DOIUrl":"10.1155/jdr/8063481","url":null,"abstract":"<p><p>It is critical to ensure that lifestyle change programs are tailored to the person with prediabetes needs and wishes. However, programs that are carried out in research settings to delay or prevent Type 2 diabetes in people with prediabetes do not translate easily to everyday settings. There is a need to explore further the perceptions of people with prediabetes about the condition and their role in self-management to better balance the content of intervention programs for prediabetes with the participants' life context and experience. For this purpose, we invited 21 persons with prediabetes from four primary healthcare centers in Region Stockholm, Sweden, for individual interviews. Transcripts were analyzed with qualitative content analysis. Two main themes were identified, <i>prediabetes: a condition between health and disease</i> and <i>I must manage prediabetes myself but need support</i>. This in-between state has a serious impact on the decisions that people with prediabetes make concerning self-management and behavioral changes. One of the main findings of this study highlights the importance of communicating the diagnosis of prediabetes clearly and the importance of preventive actions as this can trigger behavioral change. People with prediabetes in our study shed light on different needs for support to make and maintain behavioral change which requires a person-centered approach. This support was described internally, from family and peers, or externally from healthcare professionals. These results will be used in a codesign study where healthcare professionals and persons with prediabetes discuss the components of a person-centered model for a behavioral change intervention in primary healthcare.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"8063481"},"PeriodicalIF":3.6,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11839257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143458263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glucagon-Like Peptide 1 Receptor Agonists and Sodium-Glucose Cotransporter 2 Inhibitors Improve Renal Resistive Index in Patients With Type 2 Diabetes: A 26-Week Prospective Observational Real-Life Study.","authors":"Alfredo Vozza, Sara Volpe, Carlo Custodero, Valentina Colaianni, Valentina Lavarra, Domenico Triggiani, Lucilla Crudele, Alessandro Bergamasco, Gianfranco Antonica, Cosimo Tortorella, Giuseppina Piazzolla","doi":"10.1155/jdr/8182211","DOIUrl":"10.1155/jdr/8182211","url":null,"abstract":"<p><p>Diabetic kidney disease (DKD) is one of the most life-threatening complications of diabetes and a leading cause of chronic kidney disease. Glucagon-like peptide 1 receptor agonists (GLP1-RAs) or sodium-glucose cotransporter 2 inhibitors (SGLT2is) appear to improve renal outcome in patients with Type 2 diabetes (T2D). In this context, the renal resistive index (RRI) is a useful doppler measure to study DKD and predict its evolution. The aim of this work was to study the effect of treatment with GLP1-RA or SGLT2i on RRI and the relationship between RRI and glycometabolic parameters. One hundred forty-five patients with T2D were enrolled in the study and treated for 26 weeks with once-weekly GLP1-RA (38 patients with dulaglutide and 39 with semaglutide), SGLT2i (40 patients), or other therapies (28 control patients). Clinical, anthropometric, and hematochemical parameters and RRI were measured at baseline (T0) and after 6 months of treatment (T6). Changes at 6 months were studied and compared by treatment group. Patients were predominantly male (58.6%), overweight (93.0%) or frankly obese (60.0%), with hypertension (90.0%) and high (> 0.64) or pathological (> 0.7) RRI values (82.0% or 37.0%, respectively). At baseline, RRI correlated positively with age, fasting blood glucose, glycated hemoglobin (HbA1c), triglycerides, and albuminuria and negatively with estimated-glomerular filtration rate (e-GFR). At T6, patients treated with either GLP1-RA or SGLT2i showed a significant improvement in RRI but not in albuminuria or e-GFR, compared with homologous at baseline. In particular, RRI normalized in 32% and 30% of patients on therapy with GLP1-RA and SGLT2i, respectively, while remaining almost unchanged in controls. Notably, the RRI improvement was independent of age, gender, diabetes duration, and changes in BMI, waist circumference, HbA1c, and e-GFR. In conclusion, RRI can be used to detect early kidney damage and follow the evolution of DKD. GLP1-RA and SGLT2i improve RRI, demonstrating benefits on cardiovascular risk and renal outcomes.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"8182211"},"PeriodicalIF":3.6,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11832268/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143441125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Open-Label Comparative Study of the Impact of Two Types of Electrical Stimulation (Direct Current Neuromuscular Electrical Stimulation and Transcutaneous Electrical Stimulation) on Physical Therapy Treatment of Diabetic Peripheral Neuropathy.","authors":"Dimitrios Kostopoulos, Konstantine Rizopoulos, Joe McGilvrey, Jennifer Hauskey, Jeff Courcier, Kay Connor-Israel, Harry Koster, Ramona von Leden","doi":"10.1155/jdr/9970124","DOIUrl":"10.1155/jdr/9970124","url":null,"abstract":"<p><p><b>Objective:</b>The objective of this study is to evaluate and compare the effectiveness of treatments with two different electrical stimulation (e-stim) devices-pulsed direct current (DC) (Neubie) and alternating current (AC) (transcutaneous electrical stimulation (TENS))-in the treatment of symptoms for patients with diabetic peripheral neuropathy (DPN). <b>Design:</b>Randomized controlled trial (RCT) with parallel groups. <b>Methods:</b> One hundred fifty participants were recruited from 13 Hands-On Diagnostics-affiliated sites across several US locations. Participants were randomly divided into two groups for comparison-Neubie and TENS. Participants received a 30-min foot stimulation protocol with either TENS unit electrodes or Neubie electrodes. Outcome measures included the Toronto Clinical Neuropathy Score (TCNS), two-point discrimination, visual analogue scale (VAS), vibration sense (VBS), nerve conduction velocity (NCV), and nerve amplitude. The effect of the two variables on all outcome measures was determined using an analysis of covariance (ANCOVA). <b>Results:</b> The Neubie group demonstrated statistically significant improvements in TCNS for both right and left sides (<i>p</i> < 0.001), two-point discrimination of the dominant foot (<i>p</i> = 0.001), VBS (<i>p</i> = 0.022) and VAS scores (<i>p</i> = 0.009), and some but not all nerves tested by NCV (<i>p</i> < 0.05). <b>Conclusion:</b> Overall, DPN treatment with the Neubie resulted in significant improvements in several major outcome measures, whereas TENS showed no significant difference in any outcome measure. These findings support the use of DC devices as a potentially superior therapeutic treatment for neuropathy over AC devices like the TENS unit. <b>Trial Registration:</b> ClinicalTrials.gov identifier: NCT05442021.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"9970124"},"PeriodicalIF":3.6,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824710/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Positive Islet Cell Cytoplasmic Antibody and Long-Term Use of Lipid-Lowering Agents Are Positively Correlated With Peripheral Atherosclerosis in Patients With Autoimmune Diabetes: A Cross-Sectional Study.","authors":"Xinyue Chen, Jie Yu, Yiwen Liu, Xuechen Wang, Fan Ping, Wei Li, Huabing Zhang, Lingling Xu, Yuxiu Li","doi":"10.1155/jdr/1933825","DOIUrl":"10.1155/jdr/1933825","url":null,"abstract":"<p><p><b>Aims:</b> This cross-sectional study is aimed at determining whether systemic inflammation, diabetic autoantibodies, and islet <i>β</i> cell dysfunction play a role in the progression of macrovascular complications in patients with autoimmune diabetes. <b>Methods:</b> 202 patients with autoimmune diabetes aged ≥ 35 years and hospitalized in Peking Union Medical College Hospital were enrolled in this study. The patients were divided into three groups based on the severity of peripheral atherosclerosis. Biomarkers of systemic inflammation, diabetes autoantibodies, islet <i>β</i> cell function, and other covariates validated to be associated with macrovascular complications were collected. Correlations between the severity of peripheral atherosclerosis and systemic inflammation, diabetic autoantibodies, and islet β cell function were examined using an ordinal logistic regression model. <b>Results:</b> Of the enrolled patients, 39.1% were male, with a median age of 53 (43, 60) years and a diabetes duration of 96 (36, 216) months. 58 patients had no lesions in the peripheral arteries, 72 had atherosclerosis in the carotid or lower extremity arteries, and the rest had lesions in both arteries. In the multifactor ordinal logistic regression test, positive islet cell cytoplasmic antibody (ICA) and long-term use of lipid-lowering agents were independently associated with peripheral atherosclerosis after adjusting for age and diabetes duration. <b>Conclusions:</b> The correlation between positive ICA and atherosclerosis suggests inflammation at an early stage plays a role in macrovascular complications in autoimmune diabetes. The association between long-term use of lipid-lowering agents and atherosclerosis suggests the need for early screening and intervention for dyslipidemia in patients with autoimmune diabetes.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"1933825"},"PeriodicalIF":3.6,"publicationDate":"2025-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11824714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association Between Glycemic Control and Complications With Concentration of Urinary Exfoliated Proximal Tubule Kidney Cells in People With Diabetes Mellitus.","authors":"Henry H L Wu, Venkatesha Bhagavath, Long The Nguyen, Rajkumar Chinnadurai, Ewa M Goldys, Carol A Pollock, Sonia Saad","doi":"10.1155/jdr/1273073","DOIUrl":"10.1155/jdr/1273073","url":null,"abstract":"<p><p><b>Background:</b> Emerging evidence suggests cell exfoliation could be operating under the control of cell metabolism. It is unclear if there are associations between the concentration of exfoliated kidney proximal tubule cells (PTCs) in urine with glycemic control and complications. Our study is aimed at exploring this. <b>Methods:</b> Urine samples were collected from 122 adult study participants and stored at -80°C. Exfoliated PTCs were extracted from thawed urine using a validated specific immunomagnetic separation method based on anti-CD13 and anti-SGLT-2 antibodies. The number of PTCs was assessed using brightfield microscopy. Study participants were grouped into those with no diabetes mellitus (DM) and those with DM. Individuals with DM were further subgrouped into those with and without retinopathy. Adjusted Poisson regression analysis was conducted for the DM cohort, investigating associations between demographic, clinical, and biochemical parameters with mean urinary exfoliated PTCs. <b>Results:</b> The adjusted Poisson regression analysis noted sex to have a significant association with mean number of urinary exfoliated PTCs, with a lower incidence rate in males compared to females (incidence rate ratio (IRR) 0.56, 95% CI 0.35-0.89, <i>p</i> = 0.014). Each 1% increase in glycated haemoglobin (HbA1c) was associated with an increase of 1.03 times in mean exfoliated PTCs (IRR 1.03, 95% CI 1.01-1.04, <i>p</i> = 0.007), and DM patients with retinopathy had an increase of 1.68 times in mean exfoliated PTCs compared to those without retinopathy (IRR 1.68, 95% CI 1.07-2.62, <i>p</i> = 0.024). No significant associations were observed with albuminuria or estimated glomerular filtration rate (eGFR). <b>Conclusions:</b> Our results indicate increased shedding of PTCs into the urinary tract in patients with poorer glycemic control, particularly those with diabetic retinopathy and in females.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"1273073"},"PeriodicalIF":3.6,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11756946/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028935","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Prevalence and Progression of Microvascular Complications and the Interaction With Ethnicity and Socioeconomic Status in People With Type 2 Diabetes: A Systematic Review and Meta-Analysis.","authors":"Thamer Alobaid, Janaka Karalliedde, Matthew Dl O'Connell, Luigi Gnudi, Katie Sheehan, Ka Keat Lim, Salma Ayis","doi":"10.1155/jdr/3307594","DOIUrl":"10.1155/jdr/3307594","url":null,"abstract":"<p><p><b>Introduction:</b> Diabetic nephropathy (DN) and diabetic retinopathy (DR) are serious complications of type 2 diabetes mellitus (T2DM). The reported estimates of prevalence and progression of DN and DR vary widely across studies. We undertook a systematic review and meta-analysis to determine the extent to which these variations in prevalence and progression of DN and DR may relate to different ethnic groups and socioeconomic status (SES). <b>Methods:</b> We searched the databases Ovid MEDLINE, Global Health, APA Psych Info, Embase, and PubMed for publications from 2005 to September 2023, based on T2DM and DN or DR, which included patient's ethnicities and SES. Prevalence estimates were summarized by meta-analysis using random effects models for each microvascular complication, stratified by ethnicity and SES. Data on progression was summarized narratively. <b>Results:</b> Twenty-seven studies were included. The overall prevalence of DN was 18% (95% CI: 14%, 22%) with no differences noted by ethnic group. Low economic status and low education levels were associated with a 4% increased risk of DN compared to higher levels. Higher prevalence of DR was noted among the Afro-Caribbeans, 28% (95% CI: 11%, 46%), compared to the White/Caucasian 19% (95% CI: 11%, 27%), and Asian/Indo Asians 25% (95% CI: 9%, 41%). Low-SES populations have a higher prevalence of DR than high-SES populations. The average prevalence was 16% (95% CI: 11%, 22%) among the high economic status group, compared to 25% (95% CI:20%, 30%) for the low economic status. Our study showed that Black ethnicity was associated with a higher risk of progression to end-stage renal disease (ESRD) and diabetic maculopathy compared to other ethnicities. People with high SES had a lower rate of DR progression than those with low SES, odds ratio (OR) (0.63, 95% CI: 53%, 74%). <b>Conclusion:</b> Ethnicity and SES may be associated with differential risk of development and progression of DN and DR. The available evidence was limited by the number of studies and small samples for certain ethnic/socioeconomic groups.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"3307594"},"PeriodicalIF":3.6,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742076/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Exosomal miRNA and Hepatic mRNA Expression in the Dysregulation of Insulin Action in Perimenopausal Mice.","authors":"Yu Yang, Yu Chen, Changju Liu, Su Wang, Yijing Zhao, Wen Cao, Kun Wang","doi":"10.1155/jdr/6251747","DOIUrl":"10.1155/jdr/6251747","url":null,"abstract":"<p><p><b>Introduction:</b> The aim of present study was to evaluate the impact of perimenopause on insulin resistance. Specifically, insulin sensitivity was assessed in a perimenopausal mouse model treated with 4-vinylcyclohexene diepoxide (VCD), together with the changes in exosomal miRNA and hepatic mRNA expression profiles. <b>Methods:</b> Homeostasis model assessment of insulin resistance (HOMA-IR) was utilized to assess the status of insulin resistance, and insulin action was evaluated during menopausal transition. RNA sequencing (RNA-seq) analysis was used to identify altered expression profiles of exosomal miRNAs and hepatic mRNAs. Differentially expressed miRNA (DEM)-differentially expressed gene (DEG) network analyses were also conducted. Furthermore, altered expression levels of these exosomal miRNAs and genes were validated in plasma exosomes and liver tissue of perimenopausal mice. <b>Results:</b> HOMA-IR in VCD-treated mice was significantly increased, and hepatic glycogen was significantly decreased. Key exosomal miRNAs (miR-17-3p, miR-134-5p, miR-700-5p, and miR-6899-3p) and hepatic genes (<i>G6pdx</i>, <i>Ptpn2</i>, <i>Lepr</i>, <i>Kras</i>, and <i>Braf</i>) may be associated with impaired insulin signaling during perimenopause. <b>Conclusion:</b> The perimenopausal period acts as a potential factor in introducing insulin resistance as evidenced by impaired insulin action and altered expression profiles of exosomal miRNAs and hepatic genes. The present study contributes to the understanding that abnormal cargos carried by plasma exosomes, such as miRNAs, may be related to altered expression of the corresponding genes in the liver and abnormal insulin response.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"6251747"},"PeriodicalIF":3.6,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737908/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Demographic and Clinical Characteristics of Patients With Charcot Neuro-Osteoarthropathy in People With Diabetes Mellitus in Six Countries: A Multicenter Observational Study From 1996 to 2022.","authors":"E B Jude, C Siafarikas, A Rastogi, R Bem, T Tankova, M-F Kong, J LaFontaine, J Pappachan, N Tentolouris","doi":"10.1155/jdr/4275741","DOIUrl":"10.1155/jdr/4275741","url":null,"abstract":"<p><p><b>Aim:</b> To describe the demographic and clinical characteristics of patients with Charcot neuro-osteoarthropathy (CNO) and to examine for differences between participants with Type 1 diabetes mellitus (DM) (T1DM) and Type 2 diabetes mellitus (T2DM). <b>Materials and Methods:</b> Multicenter observational study in eight diabetic foot clinics in six countries between January 1, 1996, and December 31, 2022. Demographic, clinical, and laboratory parameters were obtained from the medical records. Analyses were performed using parametric or nonparametric statistical tests for variables with normally or nonnormally distributed values, respectively. Comparisons of the qualitative data were performed using the chi-square test. <b>Results:</b> Seven hundred seventy-four patients with DM and CNO were included. The mean age at diagnosis of CNO was 54.5 ± 11.7 years, and the median (interquartile range (IQR)) diabetes duration at diagnosis of CNO was 15 (10-22) years. Among participants, 71.8% (<i>n</i> = 546) were male and 83.2% (<i>n</i> = 634) had T2DM. Neuropathy was present in 91.7% (<i>n</i> = 688), retinopathy in 60.2% (<i>n</i> = 452), and nephropathy in 45.2% (<i>n</i> = 337). Subjects with T1DM, compared to T2DM, were diagnosed with CNO at a younger age (46.9 ± 11.0 vs. 57.9 ± 10.2 years, <i>p</i> < 0.001), had longer diabetes duration (median value (IQR): 29.0 (21.0-38.0) vs. 14.0 (8.0-20.0) years, <i>p</i> < 0.001), and had more often microvascular complications (neuropathy, 95.2% in T1DM vs. 87.4% in T2DM, <i>p</i> = 0.006; retinopathy, 83.3% vs. 55.4%, <i>p</i> < 0.001; and nephropathy 67.5% vs. 40.5%, <i>p</i> < 0.001). <b>Conclusions:</b> CNO is predominant in males, occurs in long-standing DM, and is often accompanied by microvascular complications. People with T1DM, compared to those with T2DM, are affected at a younger age, have longer diabetes duration, and have more often microvascular complications.</p>","PeriodicalId":15576,"journal":{"name":"Journal of Diabetes Research","volume":"2025 ","pages":"4275741"},"PeriodicalIF":3.6,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11735061/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143006370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}