Journal of Comparative Neurology最新文献

{"title":"Label-Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory-Related Brain Regions of a Miniature Transparent Vertebrate","authors":"Rose L. Tatarsky,&nbsp;Najva Akbari,&nbsp;Ke Wang,&nbsp;Chris Xu,&nbsp;Andrew H. Bass","doi":"10.1002/cne.70048","DOIUrl":"https://doi.org/10.1002/cne.70048","url":null,"abstract":"<div>\u0000 \u0000 <p>Animals integrate information from different sensory modalities as they mature and perform increasingly complex behaviors. This may parallel differential investment in specific brain regions depending on the changing demands of sensory inputs. To investigate developmental changes in the volume of canonical sensory regions, we used third harmonic generation imaging for morphometric analysis of forebrain and midbrain regions from larval through juvenile and adult stages in <i>Danionella dracula</i>, a transparent, miniature teleost fish whose brain is optically accessible throughout its lifespan. Relative to whole-brain volume, increased volume or investment in the telencephalon, a higher order sensory integration center, shows the most dramatic increases between 30–60 days postfertilization (dpf) and again at 90 dpf as animals reach adulthood. The torus longitudinalis (TL), a midbrain visuomotor integration center, also significantly increases between 60 and 90 dpf. In contrast, investment in the midbrain optic tectum (TeO), a retinal-recipient target, progressively decreases from 30 to 90 dpf, whereas investment is relatively consistent across all stages for the midbrain torus semicircularis (TS), a secondary auditory and mechanosensory lateral line center, and the olfactory bulb (OB), a direct target of the olfactory epithelium. In sum, increased investment in higher-order integration centers (telencephalon, TL) occurs as juveniles reach adulthood (60–90 dpf) and exhibit more complex cognitive tasks, whereas investment in modality-dominant regions occurs earlier (TeO) or is relatively consistent across development (TS, OB). Complete optical access throughout <i>Danionella</i>’s lifespan provides a unique opportunity to investigate how changing brain structure over development correlates with changes in connectivity, microcircuitry, or behavior.</p>\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover Image, Volume 533, Issue 2","authors":"Hideki Kondo,&nbsp;Laszlo Zaborszky","doi":"10.1002/cne.70041","DOIUrl":"https://doi.org/10.1002/cne.70041","url":null,"abstract":"<p>The cover image is based on the Research Article <i>Basal Forebrain Projections to the Retrosplenial and Cingulate Cortex in Rats</i> by Hideki Kondo et al., https://doi.org/10.1002/cne.70027.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 2","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70041","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143762018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of Sex-Steroid Receptors and Sex Differences of Otp Glutamatergic Neurons of the Medial Extended Amygdala","authors":"Alba González-Alonso,&nbsp;Lorena Morales,&nbsp;Elisenda Sanz,&nbsp;Loreta Medina,&nbsp;Ester Desfilis","doi":"10.1002/cne.70047","DOIUrl":"https://doi.org/10.1002/cne.70047","url":null,"abstract":"<div>\u0000 \u0000 <p>The medial extended amygdala (EAme) is part of the social behavior network and its subdivisions show expression of sex-steroid receptors, which participate in the regulation of sexually dimorphic behaviors. However, EAme subdivisions are highly heterogeneous in terms of neuron subtypes, with different subpopulations being involved in regulation of different aspects of social and non-social behaviors. To further understand the role of the different EAme neurons and their contribution to sexual differences, here we studied one of its major subtypes of glutamatergic neurons, those derived from the telencephalon-opto-hypothalamic domain that coexpress <i>Otp</i> and <i>Foxg1</i> genes during development. Our results showed that the vast majority of the Otp glutamatergic neurons of the medial amygdala and medial bed nucleus of the stria terminalis (BSTM) in both sexes express <i>Ar</i>, <i>Esr1 (ERα)</i>, and <i>Esr2 (ERβ</i>) mRNA. Moreover, the high percentage of receptors expression in the Otp neurons (between 93% and 100%) indicates that probably the majority of the Otp neurons of EAme are coexpressing the three receptors. In addition, Otp neurons of the posterodorsal medial amygdala have a larger soma and occupy more space in males than in females. These and other features of the Otp neurons regarding their expression of sex-steroid receptors likely contribute to some of the sexually dimorphic behaviors regulated by EAme.</p>\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evidence for Auditory Stimulus-Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains","authors":"Maya Wilde,&nbsp;Rebecca E. Poulsen,&nbsp;Wei Qin,&nbsp;Joshua Arnold,&nbsp;Itia A. Favre-Bulle,&nbsp;Jason B. Mattingley,&nbsp;Ethan K. Scott,&nbsp;Sarah J. Stednitz","doi":"10.1002/cne.70046","DOIUrl":"https://doi.org/10.1002/cne.70046","url":null,"abstract":"<p>Animals receive a constant stream of sensory input, and detecting changes in this sensory landscape is critical to their survival. One signature of change detection in humans is the auditory mismatch negativity (MMN), a neural response to unexpected stimuli that deviate from a predictable sequence. This process requires the auditory system to adapt to specific repeated stimuli while remaining sensitive to novel input (stimulus-specific adaptation [SSA]). MMN was originally described in humans, and equivalent responses have been found in other mammals and birds, but it is not known to what extent this deviance detection circuitry is evolutionarily conserved. Here we present the first evidence for SSA in the brain of a teleost fish, using whole-brain calcium imaging of larval zebrafish at single-neuron resolution with selective plane illumination microscopy. We found frequency-specific responses across the brain with variable response amplitudes for frequencies of the same volume and created a loudness curve to model this effect. We presented an auditory “oddball” stimulus in an otherwise predictable train of pure tone stimuli and did not find a population of neurons with specific responses to deviant tones that were not otherwise explained by SSA. Further, we observed no deviance responses to an unexpected omission of a sound in a repetitive sequence of white noise bursts. These findings extend the known scope of auditory adaptation and deviance responses across the evolutionary tree and lay groundwork for future studies to describe the circuitry underlying auditory adaptation at the level of individual neurons.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Additional Cover: Cover Image, Volume 533, Issue 1","authors":"Alana Rivera,&nbsp;Dina Bracho-Rincón,&nbsp;Mark W. Miller","doi":"10.1002/cne.70040","DOIUrl":"https://doi.org/10.1002/cne.70040","url":null,"abstract":"<p>The cover image is based on the Research article <i>Localization of Cholecystokinin/Sulfakinin Neuropeptides in Biomphalaria glabrata, an Intermediate Host for Schistosomiasis</i> by Alana Rivera et al., https://doi.org/10.1002/cne.70016.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 1","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70040","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Timing Matters: Lessons From Perinatal Neurogenesis in the Olfactory Bulb","authors":"Teresa Liberia,&nbsp;Kimberly Han,&nbsp;Natalie J. Spence,&nbsp;Sarah J. Meller,&nbsp;Eduardo Martin-Lopez,&nbsp;Charles A. Greer","doi":"10.1002/cne.70045","DOIUrl":"https://doi.org/10.1002/cne.70045","url":null,"abstract":"<div>\u0000 \u0000 <p>In the olfactory bulb (OB), odorant receptor-specific input converges into glomeruli. Subsequently, the coding of odor information is fine-tuned by local synaptic circuits within the glomeruli and the deeper external plexiform layer (EPL) in the OB. Deciphering the organization of inhibitory granule cells (GCs) in the EPL relative to the secondary dendrites of projection neurons is pivotal for understanding odor processing. We conducted a detailed investigation of GCs, focusing on the timing of neurogenesis, laminar distribution, and synaptogenesis between GCs and projection neurons. In summary, GCs develop following a developmental continuum with an outside-in maturation pattern from embryogenesis to adulthood. GCs born 1 week after birth display a unique sublayer-specific distribution pattern, marking a transition between embryonic or neonatal and adult stages. Integration into reciprocal synaptic circuits occurred 10 days post-neurogenesis. We conclude that the timing of neurogenesis dictates the anatomical configuration of GCs within the OB, which, in turn, regulates preferential synaptic integration with either mitral cell or tufted cell secondary dendrites.</p>\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Rapid Heat-Enhanced Golgi-Cox Staining Method for Detailed Neuroanatomical Analysis Coupled With Immunostaining","authors":"Da-Jiang Hui,&nbsp;Mei-Xue Yuan,&nbsp;Xin-Ya Qin,&nbsp;An-Qi Zhang,&nbsp;Chen-Wei Wang,&nbsp;Yu Wang,&nbsp;Jiang-Ning Zhou,&nbsp;Peng Chen,&nbsp;Qing-Hong Shan","doi":"10.1002/cne.70042","DOIUrl":"https://doi.org/10.1002/cne.70042","url":null,"abstract":"<div>\u0000 \u0000 <p>The Golgi-Cox staining technique is renowned for its ability to delineate neuronal architecture with remarkable precision. However, the traditional protocol's lengthy processing timeline and limited compatibility with immunostaining and transgenic labeling have hindered its widespread adoption in modern neuroscience research.</p>\u0000 <p>In the current study, we found that adjusting the incubation temperature to 55°C significantly reduced the staining duration to a mere 24 h for 100 µm-thick sections of mouse brain tissue. Importantly, our optimized protocol is compatible with immunostaining techniques and transgenic mouse models. In addition, using a lipopolysaccharides-induced mouse model of depression, we found a reduction in dendritic spines labeled by Golgi-Cox staining and an increase in the number of microglial cells labeled by immunofluorescence in the same samples, in addition, cross-talk between Golgi-Cox-stained neurons and microglial fibers were observed.</p>\u0000 <p>In conclusion, the modified Golgi-Cox staining technique allows for the acquisition of a more comprehensive set of data from the same biological tissue with increased efficiency. This advancement promises to improve methodologies in histopathology and neurobiology, making advanced applications of Golgi-Cox staining more accessible in contemporary neuroscience research.</p>\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental Enrichment Increases Brain Volume in Snakes","authors":"Gokulan Nagabaskaran,&nbsp;Vijay Moonilal,&nbsp;Morgan Skinner,&nbsp;Noam Miller","doi":"10.1002/cne.70043","DOIUrl":"10.1002/cne.70043","url":null,"abstract":"<p>The effects of environmental enrichment have been well documented in mammals and birds, but less work has focused on reptiles. Because snakes are common in captivity, both as pets and in research/commercial facilities, it is critical to explore how they react to standard captive housing. Here, we examined the effects of environmental enrichment on brain development in a popular pet snake species, the western hognose snake (<i>Heterodon nasicus</i>). Hognose snakes (<i>n</i> = 15) were individually housed for one year in either enriched or standard environments before their brains were harvested and imaged using MRI. We found that enriched snakes had significantly larger brain volumes compared to standard snakes, most prominently in posterior brain regions. In addition, we observed sex-specific brain investments: as snakes grew larger, males displayed relatively larger cerebral hemispheres, and females displayed larger posterior brain regions. These results suggest that environmental enrichment is critical to encouraging healthy brain development in snakes and that snake brain plasticity is very similar to that observed in mammals and birds.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating Neotenic and Metamorphic Axolotl Brain Complexity: A Stereological and Immunohistochemical Perspective","authors":"Arife Ahsen Kaplan,&nbsp;Gürkan Öztürk,&nbsp;Sadık Bay,&nbsp;İlknur Keskin","doi":"10.1002/cne.70031","DOIUrl":"10.1002/cne.70031","url":null,"abstract":"<p>The ability of certain tetrapods, such as amphibians, to regenerate complex structures, such as organs or limbs, is well-established, though this capacity varies significantly across species, with humans exhibiting limited regenerative potential. Ependymoglia cells in the ventricular region of the brain are known to exhibit proliferative properties during homeostasis and damage and to perform stem cell functions. This study investigated changes occurring in neurons and glia in the central nervous system following metamorphosis in axolotls. Morphological alterations in brain tissue, newly formed neurons, and cellular organizations in different brain regions were assessed using stereological and immunohistochemical methods, as well as light and electron microscopy. Interestingly, we observe no statistically significant difference in total neuron numbers in the telencephalon region between neotenic and metamorphic axolotls. However, the proliferation index and the numbers of cells expressing NeuN were significantly higher in metamorphic axolotls. Furthermore, structural changes in neuronal nuclei and myelin sheath organization were determined at the light and electron microscopic levels post-metamorphosis. Ultrastructural analyses revealed a change in chromatin organization from euchromatic to heterochromatic in neurons after metamorphosis, and morphological changes were also demonstrated in myelinated nerve fibers in the telencephalon. Additionally, mucopolysaccharide-containing secretory sacs were also identified on the apical surfaces of a subgroup of ependymoglia cells located in the lateral ventricle wall. Overall, this study sheds useful light on the intricate changes occurring in the central nervous system during metamorphosis in axolotls and provides valuable insights into the mechanisms underlying these processes.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11923732/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptomic and Histological Characterization of Telocytes in the Human Dorsal Root Ganglion","authors":"Rainer V. Haberberger,&nbsp;Dusan Matusica,&nbsp;Stephanie Shiers,&nbsp;Ishwarya Sankaranarayanan,&nbsp;Theodore J. Price","doi":"10.1002/cne.70044","DOIUrl":"https://doi.org/10.1002/cne.70044","url":null,"abstract":"<p>Telocytes are interstitial cells characterized by long processes that span considerable distances within tissues, likely facilitating coordination and interaction with various cell types. Although present in central and peripheral neuronal tissues, their role remains elusive. Dorsal root ganglia (DRG) house pseudounipolar afferent neurons responsible for transmitting signals related to temperature, proprioception, and nociception. This study aimed to investigate the presence and function of telocytes in human DRG by examining their transcriptional profile, anatomical location, and ultrastructure.</p><p>Combined expression of <i>CD34</i> and <i>PDGFRA</i> is a marker gene set for telocytes, and our sequencing data revealed CD34 and PDGFRA expressing cells comprise roughly 1.5%–3% of DRG cells. Combined expression of <i>CD34</i> and <i>PDGFRA</i> is a putative marker gene set for telocytes. Further analysis identified nine subclusters with enriched cluster-specific genes. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway analysis suggested vascular, immune, and connective tissue-associated putative telocyte subtypes, mapping over 3000 potential receptor–ligand interactions between sensory neurons and these <i>CD34</i> and <i>PDGFRA</i> expressing putative telocytes were identified using a ligand–receptors interactome platform. Immunohistochemistry identified CD34+ve telocytes in the endoneural space of DRGs, next to neuron–satellite complexes, in perivascular spaces and in the endoneural space between nerve fiber bundles, consistent with pathway analysis. Transmission electron microscopy (TEM) confirmed their location identifying characteristic elongated nucleus, long and thin telopodes containing vesicles, often surrounded by a basal lamina. This study provides the first gene expression analysis of telocytes in complex human tissue, specifically the DRG, highlighting functional differences based on tissue location while revealing no significant ultrastructural variations.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70044","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}