Journal of Comparative Neurology最新文献

{"title":"Cover Image, Volume 533, Issue 7","authors":"Lucía Inés Torrijos-Saiz,&nbsp;Júlia Freixes,&nbsp;Ester Desfilis,&nbsp;Loreta Medina,&nbsp;Kazunobu Sawamoto,&nbsp;José Manuel García-Verdugo,&nbsp;Vicente Herranz-Pérez","doi":"10.1002/cne.70076","DOIUrl":"https://doi.org/10.1002/cne.70076","url":null,"abstract":"<p>The cover image is based on the Research Article <i>Cellular Organization and Migration Pathways of the Ventricular–Subventricular Zone in the Juvenile Swine Brain Sus scrofa domesticus</i> by Lucía Inés Torrijos-Saiz et al., https://doi.org/10.1002/cne.70070.\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70076","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144635370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Connectivity of a Conspicuous Layered Structure in the Dorsal Telencephalon of the Peacock Gudgeon, Tateurndina ocellicauda","authors":"Ruth Gutjahr,&nbsp;Maximilian S. Bothe,&nbsp;Michael H. Hofmann,&nbsp;Boris P. Chagnaud","doi":"10.1002/cne.70064","DOIUrl":"https://doi.org/10.1002/cne.70064","url":null,"abstract":"<p>Within the mammalian pallium, layered structures, such as the six-layered isocortex and the three-layered hippocampal formation, are crucial for integrating sensory cues from the environment and for forming and recalling memories. Similar layered pallial systems have also been shown in avian and non-avian reptiles. Despite sharing similar needs for processing external information and remembering important sites, teleosts have generally not evolved such defined layered organizations in their dorsal telencephalon. One exception is gobiiform fishes in which a subregion of the dorsal telencephalon is organized into several fiber-rich and soma-dense layered subregions. We investigated the connectivity of these layered subregions (referred to as the dorsal telencephalic area X, Dx), as well as the connectivity of the medial (Dm) and dorsolateral (dDl) parts of the dorsal telencephalon through tracer injections. We found that extratelencephalic projections reach Dm, Dx, and dDl from different regions within the preglomerular complex (PG): Dm receives input from different PG regions: PG region 1 (PG1), PG region 2 (PG2), and the commissural PG (PGc), but not from the nucleus prethalamicus (PTh). In contrast, both Dx and dDl receive projections from the lateral PTh (PTh-l) and the medial PTh (PTh-m). We find that projections to dDl come from more ventral regions of PTh-m than those that project to Dx. The majority of ascending connections could be found within the telencephalon itself, with each of the telencephalic zones receiving its own distinct pattern of intratelencephalic afferent connectivity. From our results, we conclude that Dm and Dx constitute two distinct zones of the dorsal telencephalon.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144615308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Brain of the African Wild Dog. V. The Somatosensory System and Vestibular Nuclear Complex","authors":"Samson Chengetanai,&nbsp;Adhil Bhagwandin,&nbsp;Mads F. Bertelsen,&nbsp;Therese Hård,&nbsp;Patrick R. Hof,&nbsp;Muhammad A. Spocter,&nbsp;Paul R. Manger","doi":"10.1002/cne.70071","DOIUrl":"https://doi.org/10.1002/cne.70071","url":null,"abstract":"<p>Social behaviors in the African wild dog (<i>Lycaon pictus</i>) commonly involve a range of tactile aspects, including biting, pushing, embracing, mounting, face and muzzle licking, nose–chin and muzzle contact, paw placement, play fighting, and wrestling, supported by the vestibular system. We employed an array of architectural and immunohistochemical stains to provide a qualitative description of the somatosensory and vestibular systems in the brain of one representative African wild dog individual. The appearance of both systems does not appear to differ from that reported in other Carnivora. The six nuclei forming the vestibular system, and their relationship to each other and the incoming vestibular branch of the eighth cranial nerve, appear like those observed in many mammalian species. The location and appearance of the dorsal column nuclei, the trigeminal sensory column, the colliculi, somatosensory nuclei of the dorsal thalamus, and the five somatosensory cortical areas observed in the African wild dog are like those observed in the domestic dog and other Carnivora. This study of the somatosensory and vestibular systems of the African wild dog completes our series of studies describing the major sensory systems in the African wild dog brain. It appears reasonable to conclude that, at the systems level of analysis, no overt specializations of any of the sensory systems are present. Thus, the neural underpinnings of the complex sociality of the African wild dog may be supported by nonsensory neural systems, such as motor, neuromodulatory, limbic, or cognitive systems, or levels of organization like receptor expression patterns or connectivity.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70071","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144598455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"“Of Marine Mammal Neuroscience and Men”: Needs and Perspectives in Marine Mammal Neuroscience","authors":"Ksenia Orekhova,&nbsp;Mark Dagleish,&nbsp;Nina Patzke,&nbsp;Simona Sacchini,&nbsp;Federica Giorda,&nbsp;Giovanni Di Guardo,&nbsp;Camilla Testori,&nbsp;Alice Affatati,&nbsp;Tommaso Gerussi,&nbsp;Mari Ochiai,&nbsp;Jean-Marie Graïc","doi":"10.1002/cne.70067","DOIUrl":"https://doi.org/10.1002/cne.70067","url":null,"abstract":"<p>As neuroscience techniques become increasingly sophisticatedand accessible, their application to marine mammal research remainsunderdeveloped and fragmented. Cetacean and pinniped brains exhibit remarkableevolutionary specializations; yet systematic, reproducible data across speciesare scarce. Ethical, logistical, and methodological constraints hinder samplingand analysis of central nervous system tissues, often limiting studies to smallcohorts and reducing diagnostic accuracy in neuropathological investigations.Gaps persist in understanding neuroanatomical organization, pathogeneticmechanisms of neurodegeneration, and the effects of acoustic and environmentalstressors on brain health. Noninvasive neuroimaging methods such as post-mortemmagnetic resonance imaging and diffusion-weighted imaging offer promise butsuffer from incompatible protocols and limited standardization. In-vitro andmolecular techniques including cellular reprogramming may provide new avenuesfor translational research if harmonized approaches are adopted. We identify a criticalneed for coordinated efforts to standardize best practice protocols for the sampling, storage and systematic analyses of marine mammal nervous tissues. To this end, we propose the formation of an inclusive, multidisciplinary network and invitecollaboration through our Open Science Framework project. By aligning methodologies and broadeninginternational partnerships, we aim to transform marine mammal neuroscience intoa robust contributor to comparative neurobiology and environmental healthmonitoring. This is a call to action to collectively grow this emerging field.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70067","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144582188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular Organization and Migration Pathways of the Ventricular–Subventricular Zone in the Juvenile Swine Brain (Sus scrofa domesticus)","authors":"Lucía Inés Torrijos-Saiz,&nbsp;Júlia Freixes,&nbsp;Ester Desfilis,&nbsp;Loreta Medina,&nbsp;Kazunobu Sawamoto,&nbsp;José Manuel García-Verdugo,&nbsp;Vicente Herranz-Pérez","doi":"10.1002/cne.70070","DOIUrl":"https://doi.org/10.1002/cne.70070","url":null,"abstract":"<p>The ventricular–subventricular zone (V-SVZ), lining the lateral walls of the lateral ventricles, is a major neurogenic region in the adult brain of many mammals. This study investigates the structural organization and cellular composition of the V-SVZ in the juvenile swine brain (3–5 months), providing novel insights into neuroblast migration in gyrencephalic species. Using immunohistochemistry combined with transmission and scanning electron microscopy, we redefined the cytoarchitecture of the swine V-SVZ, identifying four distinct cellular layers. Layer 1 consists of a pseudostratified epithelium of glial fibrillary acidic protein-positive ependymal cells, whose cilia and microvilli extend into the ventricular lumen, frequently surrounding supraependymal axons. Beneath it, layer 2 is composed of astrocytic and radial glia processes and contains occasional clusters of doublecortin (DCX)-positive cells with prominent microtubules and elongated cytoplasm, indicative of a migratory phenotype. Layer 3 is further subdivided into a low-cell-density sublayer 3a, enriched with myelinated axons and scattered DCX⁺ clusters, and a high-cell-density sublayer 3b, characterized by large groups of DCX⁺ migratory cells. In sagittal sections, these cells form long chains oriented parallel to the ventricular surface. Neuroblasts emerging from the dorsal V-SVZ migrate caudorostrally through the rostral migratory stream toward the olfactory bulb. The layered organization of the swine V-SVZ resembles that of humans, where DCX⁺ chains persist up to 18 months of age, positioning the swine as a valuable model for investigating postnatal plasticity and neurogenic potential in gyrencephalic brains. The persistence of immature neurons in the V-SVZ of gyrencephalic mammals, including infant humans, underscores the relevance of this region for neurogenesis and plasticity in large-brained species.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70070","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lateral Thinking: Exploring the Lateral Entorhinal Cortex Projections to the Hippocampus in the Rodent Brain","authors":"Rebecca Przy,&nbsp;Mira M. M. Wade,&nbsp;Priscilla G. Welter,&nbsp;Patricia S. Brocardo,&nbsp;Brian R. Christie","doi":"10.1002/cne.70069","DOIUrl":"https://doi.org/10.1002/cne.70069","url":null,"abstract":"<p>The lateral entorhinal cortex (LEC) projections to the hippocampus (HC) play a critical role in cognition and have been associated with cognitive impairments related to aging and neurodegenerative disease. This review aims to provide a detailed anatomical description of the LEC-to-HC pathway, based on a review of data collected using neuroanatomical tract tracing techniques. The LEC projects to the HC in a layer-specific manner. Layer II of the LEC provides the primary source of projections to the dentate gyrus (DG), while layer III is the primary source of projections to the subiculum and cornu Ammonis (CA) region. These projections terminate in the molecular layers of the HC and follow a topographical distribution along the dorsolateral–ventromedial axis. Having a clear understanding of the LEC-to-HC pathway is essential background for investigating its functions and alterations due to disease and aging processes.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 7","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70069","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144520013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of Postnatal Retinal Input Perturbs Cortical Lamination in the Developing Ferret Visual Cortex","authors":"Connor Hilts,&nbsp;Sarah E. Santiago,&nbsp;Christopher D. Kroenke,&nbsp;Anthony P. Barnes","doi":"10.1002/cne.70061","DOIUrl":"https://doi.org/10.1002/cne.70061","url":null,"abstract":"<div>\u0000 \u0000 <p>Neuroanatomical development of the visual system is influenced by activity-dependent processes. Removal of retinal input early in development in animal model experiments reproduces several structural characteristics of the primary visual cortex in humans who have been blinded early in life. Yet, many questions regarding how the loss of retinal activity affects the organization and cellular composition of the visual cortex remain to be answered. Here, we report alterations to primary visual cortex development in ferrets over the postnatal day 20 (P20) to P38 age range that arise following bilateral enucleation on P7. Most notably, during this age range, V1 is observed to become thicker in P7 enucleates than in control animals. While this effect recapitulates observations of abnormally thick visual cortex in early blind humans, the observation that the thickness difference is observable by P32, while the cortex is still developing, has implications that run counter to prevailing interpretations of the V1 thickness difference. In order to further characterize the cytoarchitectural development of V1, we quantify the number and distribution of the two largest populations of inhibitory neurons through postnatal development, illustrating how the emergence of visual input–dependent organization displays a unique time course in both controls and enucleates. We observe perturbations to macroscopic anatomical development with only minor effects on the number and distribution of interneurons present throughout late corticogenesis following binocular enucleation. Our results demonstrate that postnatal enucleation can produce substantial and durable alterations to the mature organization of the visual cortex, likely mirroring those occurring in human patients who lose sight early in life. Our findings identify specific cellular and molecular alterations and highlight challenges that will need to be addressed in the design and development of restorative therapeutics.</p>\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144367486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Method for Culturing Telencephalic Neurons in Axolotls","authors":"Sevginur Bostan,&nbsp;Safiye Serdengeçti,&nbsp;F. Kemal Bayat,&nbsp;Sadık Bay,&nbsp;AyşeServer Sezer,&nbsp;Neşe Ayşit,&nbsp;Gürkan Öztürk","doi":"10.1002/cne.70066","DOIUrl":"https://doi.org/10.1002/cne.70066","url":null,"abstract":"<p>The axolotl (<i>Ambystoma mexicanum</i>), a neotenic salamander with remarkable regenerative capabilities, serves as a key model for studying nervous system regeneration. Despite its potential, the cellular and molecular mechanisms underlying this regenerative capacity remain poorly understood, partly due to the lack of reliable in vitro models for axolotl neural cells. In this study, we developed a novel protocol for primary cultures of adult axolotl telencephalon/pallium, enabling the maintenance of viable and functionally active neural cells. Using calcium imaging and immunocytochemistry, we demonstrated the presence of neuronal and glial markers, synaptic connections, and spontaneous calcium activity, highlighting the functional integrity of the cultured cells. Our findings reveal that these cultures can be maintained in both serum and serum-free conditions, with neurons exhibiting robust neurite outgrowth and responsiveness to injury. This protocol addresses a critical gap in axolotl research by providing a controlled in vitro system to study neurogenesis and regeneration. By offering insights into the regenerative mechanisms of axolotl neurons, this work lays the foundation for comparative studies with mammalian systems, potentially informing therapeutic strategies for neurodegenerative diseases and CNS injuries in humans.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70066","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144315006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Canonical Proprioceptors Are Largely Absent in the Intrinsic Laryngeal Muscles of the Rat Larynx","authors":"Victoria X. Yu,&nbsp;Ignacio Hernández-Morato,&nbsp;Susan Brenner-Morton,&nbsp;Charlotte L. West,&nbsp;Yalda Moayedi,&nbsp;Michael J. Pitman","doi":"10.1002/cne.70062","DOIUrl":"https://doi.org/10.1002/cne.70062","url":null,"abstract":"<p>Proprioception plays a crucial role in laryngeal function for voicing and swallowing. Despite this, the physiology of laryngeal proprioception is not well-understood, and controversy remains over whether canonical proprioceptive organs, like muscle spindles, even exist in the intrinsic laryngeal muscles. This study's primary aim is to determine whether the intrinsic laryngeal muscles contain muscle spindles and Golgi tendon organs using VGLUT1, a marker for proprioceptors. This is a novel approach, as prior studies have relied on morphology and myosin composition to study this question. Larynges of 62 Sprague-Dawley rats distributed across five age groups were immunostained with VGLUT1 and other neuromarkers. Muscle spindles were identified in the lateral thyroarytenoid muscles of just three P8 rats, and no Golgi tendon organs were noted. VGLUT1-positive intramuscular receptor-like entities and flower spray-like endings were found in the intrinsic laryngeal muscles, and mucosal formations were observed clustered at the medial surfaces of the arytenoid and aryepiglottic folds. Employing VGLUT1 immunostaining, this study shows that rat intrinsic laryngeal muscles rarely contain muscle spindles and do not possess Golgi tendon organs. This suggests the possibility that the larynx exhibits a unique proprioceptive apparatus. VGLUT1-positive intramuscular and mucosal structures provide candidates for an alternative system. Further defining the role of these structures will increase our understanding of vocal fold function and ultimately lead to better treatment of vocal fold disorders.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70062","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144300117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neural Repertoire Behind the World's Most Complex Retina: Neuroanatomy of the Stomatopod Lamina","authors":"Amy Streets,&nbsp;Hayley England,&nbsp;Justin Marshall","doi":"10.1002/cne.70063","DOIUrl":"https://doi.org/10.1002/cne.70063","url":null,"abstract":"<p>The lamina is the first optic neuropil and visual information integration station in crustaceans and insects, containing synaptic connections between photoreceptors and first-order interneurons. The lamina circuitry in mantis shrimp (stomatopods) is both interesting and complex, as there are 16 different types of photoreceptors contained within the mid-band region of the eye. Using serial block-face scanning electron microscopy, we have reconstructed photoreceptor terminals and lamina interneurons in two major superfamilies with different visual ecologies. Neurons follow the same general pattern as other crustaceans but with notable differences in gross anatomy from insects. The photoreceptors form bulbous terminals in the lamina, following the same overall connectivity pattern in all lamina cartridges across species examined and eye regions. The photoreceptor terminals themselves appear to be complex, with many large mitochondria, a notable difference between insects and stomatopods. Connectivity between photoreceptors and interneurons, as well as cross-cartridge connections, is estimated based on neuronal overlap. Lamina monopolar cells follow previous research in stomatopods and crustaceans, with a set of common neuron types that may provide the beginning of an opponency circuit. Additionally, neurons that extend beyond their parent cartridge in the midband show a preference for branching between rows within the same visual column of cells, as well as some connections within the same row. This added complexity suggests that the stomatopod lamina performs a unique processing of visual signals versus other crustaceans and insects and provides further evidence for the emerging hypotheses around the processing of information by the scanning visual system of stomatopods.</p>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 6","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70063","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144264499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}