Journal of Comparative Neurology最新文献

Nerve Growth Factor Receptor (NGFR/p75NTR) of the Small-Spotted Catshark (Scyliorhinus canicula): Evolutionary Conservation and Brain Function

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-04-12 DOI: 10.1002/cne.70049

Elena Chiavacci, Roberta Camera, Mario Costa, Baldassare Fronte, Eva Terzibasi Tozzini, Alessandro Cellerino

{"title":"Nerve Growth Factor Receptor (NGFR/p75NTR) of the Small-Spotted Catshark (Scyliorhinus canicula): Evolutionary Conservation and Brain Function","authors":"Elena Chiavacci, Roberta Camera, Mario Costa, Baldassare Fronte, Eva Terzibasi Tozzini, Alessandro Cellerino","doi":"10.1002/cne.70049","DOIUrl":"https://doi.org/10.1002/cne.70049","url":null,"abstract":"The p75NTR receptor, a member of the tumor necrosis factor (TNF) receptor superfamily, can participate in signaling pathways either by forming heteromeric complexes with other receptors, such as the Trk family (tropomyosin receptor kinases), or by functioning independently. p75NTR was investigated prevalently in the brain and retina of mammals, whereas almost nothing is known about its conservation among species. Here, we reconstructed the phylogenetic arb of p75NTR and described for the first time the p75NTR expression in the brain of the basal vertebrate Chondrichthyan Scyliorhinus canicula (S. canicula), uncovering the existing parallelism between ancient vertebrates and mammals. p75NTR functional conservation among vertebrates was further investigated by cloning the S. canicula nerve growth factor (NGF) and performing the canonical posterior commissure (PC)-12 differentiation assay, which results in standard neurite-like production. We then investigated the S. canicula p75NTR, which proves to be capable of complementing a specific clone of PC-12 lacking p75NTR (PC-12 p75NTR−/−). All together, our results highlighted the expression and functional conservation of p75NTR among vertebrates during the evolution.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70049","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143824716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Image, Volume 533, Issue 4

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-04-10 DOI: 10.1002/cne.70052

Da-Jiang Hui, Mei-Xue Yuan, Xin-Ya Qin, An-Qi Zhang, Chen-Wei Wang, Yu Wang, Jiang-Ning Zhou, Peng Chen, Qing-Hong Shan

引用次数: 0

Label-Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory-Related Brain Regions of a Miniature Transparent Vertebrate

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-04-09 DOI: 10.1002/cne.70048

Rose L. Tatarsky, Najva Akbari, Ke Wang, Chris Xu, Andrew H. Bass

{"title":"Label-Free Multiphoton Imaging Reveals Volumetric Shifts Across Development in Sensory-Related Brain Regions of a Miniature Transparent Vertebrate","authors":"Rose L. Tatarsky, Najva Akbari, Ke Wang, Chris Xu, Andrew H. Bass","doi":"10.1002/cne.70048","DOIUrl":"https://doi.org/10.1002/cne.70048","url":null,"abstract":"<div>\u0000 \u0000 Animals integrate information from different sensory modalities as they mature and perform increasingly complex behaviors. This may parallel differential investment in specific brain regions depending on the changing demands of sensory inputs. To investigate developmental changes in the volume of canonical sensory regions, we used third harmonic generation imaging for morphometric analysis of forebrain and midbrain regions from larval through juvenile and adult stages in Danionella dracula, a transparent, miniature teleost fish whose brain is optically accessible throughout its lifespan. Relative to whole-brain volume, increased volume or investment in the telencephalon, a higher order sensory integration center, shows the most dramatic increases between 30–60 days postfertilization (dpf) and again at 90 dpf as animals reach adulthood. The torus longitudinalis (TL), a midbrain visuomotor integration center, also significantly increases between 60 and 90 dpf. In contrast, investment in the midbrain optic tectum (TeO), a retinal-recipient target, progressively decreases from 30 to 90 dpf, whereas investment is relatively consistent across all stages for the midbrain torus semicircularis (TS), a secondary auditory and mechanosensory lateral line center, and the olfactory bulb (OB), a direct target of the olfactory epithelium. In sum, increased investment in higher-order integration centers (telencephalon, TL) occurs as juveniles reach adulthood (60–90 dpf) and exhibit more complex cognitive tasks, whereas investment in modality-dominant regions occurs earlier (TeO) or is relatively consistent across development (TS, OB). Complete optical access throughout Danionella’s lifespan provides a unique opportunity to investigate how changing brain structure over development correlates with changes in connectivity, microcircuitry, or behavior.\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143809451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Image, Volume 533, Issue 2

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-04-03 DOI: 10.1002/cne.70041

Hideki Kondo, Laszlo Zaborszky

引用次数: 0

Expression of Sex-Steroid Receptors and Sex Differences of Otp Glutamatergic Neurons of the Medial Extended Amygdala

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-04-02 DOI: 10.1002/cne.70047

Alba González-Alonso, Lorena Morales, Elisenda Sanz, Loreta Medina, Ester Desfilis

{"title":"Expression of Sex-Steroid Receptors and Sex Differences of Otp Glutamatergic Neurons of the Medial Extended Amygdala","authors":"Alba González-Alonso, Lorena Morales, Elisenda Sanz, Loreta Medina, Ester Desfilis","doi":"10.1002/cne.70047","DOIUrl":"https://doi.org/10.1002/cne.70047","url":null,"abstract":"<div>\u0000 \u0000 The medial extended amygdala (EAme) is part of the social behavior network and its subdivisions show expression of sex-steroid receptors, which participate in the regulation of sexually dimorphic behaviors. However, EAme subdivisions are highly heterogeneous in terms of neuron subtypes, with different subpopulations being involved in regulation of different aspects of social and non-social behaviors. To further understand the role of the different EAme neurons and their contribution to sexual differences, here we studied one of its major subtypes of glutamatergic neurons, those derived from the telencephalon-opto-hypothalamic domain that coexpress Otp and Foxg1 genes during development. Our results showed that the vast majority of the Otp glutamatergic neurons of the medial amygdala and medial bed nucleus of the stria terminalis (BSTM) in both sexes express Ar, Esr1 (ERα), and Esr2 (ERβ) mRNA. Moreover, the high percentage of receptors expression in the Otp neurons (between 93% and 100%) indicates that probably the majority of the Otp neurons of EAme are coexpressing the three receptors. In addition, Otp neurons of the posterodorsal medial amygdala have a larger soma and occupy more space in males than in females. These and other features of the Otp neurons regarding their expression of sex-steroid receptors likely contribute to some of the sexually dimorphic behaviors regulated by EAme.\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143749300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evidence for Auditory Stimulus-Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-03-26 DOI: 10.1002/cne.70046

Maya Wilde, Rebecca E. Poulsen, Wei Qin, Joshua Arnold, Itia A. Favre-Bulle, Jason B. Mattingley, Ethan K. Scott, Sarah J. Stednitz

{"title":"Evidence for Auditory Stimulus-Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains","authors":"Maya Wilde, Rebecca E. Poulsen, Wei Qin, Joshua Arnold, Itia A. Favre-Bulle, Jason B. Mattingley, Ethan K. Scott, Sarah J. Stednitz","doi":"10.1002/cne.70046","DOIUrl":"https://doi.org/10.1002/cne.70046","url":null,"abstract":"Animals receive a constant stream of sensory input, and detecting changes in this sensory landscape is critical to their survival. One signature of change detection in humans is the auditory mismatch negativity (MMN), a neural response to unexpected stimuli that deviate from a predictable sequence. This process requires the auditory system to adapt to specific repeated stimuli while remaining sensitive to novel input (stimulus-specific adaptation [SSA]). MMN was originally described in humans, and equivalent responses have been found in other mammals and birds, but it is not known to what extent this deviance detection circuitry is evolutionarily conserved. Here we present the first evidence for SSA in the brain of a teleost fish, using whole-brain calcium imaging of larval zebrafish at single-neuron resolution with selective plane illumination microscopy. We found frequency-specific responses across the brain with variable response amplitudes for frequencies of the same volume and created a loudness curve to model this effect. We presented an auditory “oddball” stimulus in an otherwise predictable train of pure tone stimuli and did not find a population of neurons with specific responses to deviant tones that were not otherwise explained by SSA. Further, we observed no deviance responses to an unexpected omission of a sound in a repetitive sequence of white noise bursts. These findings extend the known scope of auditory adaptation and deviance responses across the evolutionary tree and lay groundwork for future studies to describe the circuitry underlying auditory adaptation at the level of individual neurons.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cne.70046","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Additional Cover: Cover Image, Volume 533, Issue 1

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-03-25 DOI: 10.1002/cne.70040

Alana Rivera, Dina Bracho-Rincón, Mark W. Miller

引用次数: 0

Timing Matters: Lessons From Perinatal Neurogenesis in the Olfactory Bulb

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-03-24 DOI: 10.1002/cne.70045

Teresa Liberia, Kimberly Han, Natalie J. Spence, Sarah J. Meller, Eduardo Martin-Lopez, Charles A. Greer

{"title":"Timing Matters: Lessons From Perinatal Neurogenesis in the Olfactory Bulb","authors":"Teresa Liberia, Kimberly Han, Natalie J. Spence, Sarah J. Meller, Eduardo Martin-Lopez, Charles A. Greer","doi":"10.1002/cne.70045","DOIUrl":"https://doi.org/10.1002/cne.70045","url":null,"abstract":"<div>\u0000 \u0000 In the olfactory bulb (OB), odorant receptor-specific input converges into glomeruli. Subsequently, the coding of odor information is fine-tuned by local synaptic circuits within the glomeruli and the deeper external plexiform layer (EPL) in the OB. Deciphering the organization of inhibitory granule cells (GCs) in the EPL relative to the secondary dendrites of projection neurons is pivotal for understanding odor processing. We conducted a detailed investigation of GCs, focusing on the timing of neurogenesis, laminar distribution, and synaptogenesis between GCs and projection neurons. In summary, GCs develop following a developmental continuum with an outside-in maturation pattern from embryogenesis to adulthood. GCs born 1 week after birth display a unique sublayer-specific distribution pattern, marking a transition between embryonic or neonatal and adult stages. Integration into reciprocal synaptic circuits occurred 10 days post-neurogenesis. We conclude that the timing of neurogenesis dictates the anatomical configuration of GCs within the OB, which, in turn, regulates preferential synaptic integration with either mitral cell or tufted cell secondary dendrites.\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid Heat-Enhanced Golgi-Cox Staining Method for Detailed Neuroanatomical Analysis Coupled With Immunostaining

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-03-24 DOI: 10.1002/cne.70042

Da-Jiang Hui, Mei-Xue Yuan, Xin-Ya Qin, An-Qi Zhang, Chen-Wei Wang, Yu Wang, Jiang-Ning Zhou, Peng Chen, Qing-Hong Shan

{"title":"A Rapid Heat-Enhanced Golgi-Cox Staining Method for Detailed Neuroanatomical Analysis Coupled With Immunostaining","authors":"Da-Jiang Hui, Mei-Xue Yuan, Xin-Ya Qin, An-Qi Zhang, Chen-Wei Wang, Yu Wang, Jiang-Ning Zhou, Peng Chen, Qing-Hong Shan","doi":"10.1002/cne.70042","DOIUrl":"https://doi.org/10.1002/cne.70042","url":null,"abstract":"<div>\u0000 \u0000 The Golgi-Cox staining technique is renowned for its ability to delineate neuronal architecture with remarkable precision. However, the traditional protocol's lengthy processing timeline and limited compatibility with immunostaining and transgenic labeling have hindered its widespread adoption in modern neuroscience research.\u0000 In the current study, we found that adjusting the incubation temperature to 55°C significantly reduced the staining duration to a mere 24 h for 100 µm-thick sections of mouse brain tissue. Importantly, our optimized protocol is compatible with immunostaining techniques and transgenic mouse models. In addition, using a lipopolysaccharides-induced mouse model of depression, we found a reduction in dendritic spines labeled by Golgi-Cox staining and an increase in the number of microglial cells labeled by immunofluorescence in the same samples, in addition, cross-talk between Golgi-Cox-stained neurons and microglial fibers were observed.\u0000 In conclusion, the modified Golgi-Cox staining technique allows for the acquisition of a more comprehensive set of data from the same biological tissue with increased efficiency. This advancement promises to improve methodologies in histopathology and neurobiology, making advanced applications of Golgi-Cox staining more accessible in contemporary neuroscience research.\u0000 </div>","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 4","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143689964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Environmental Enrichment Increases Brain Volume in Snakes

IF 2.3 4区医学

Journal of Comparative Neurology Pub Date : 2025-03-21 DOI: 10.1002/cne.70043

Gokulan Nagabaskaran, Vijay Moonilal, Morgan Skinner, Noam Miller

{"title":"Environmental Enrichment Increases Brain Volume in Snakes","authors":"Gokulan Nagabaskaran, Vijay Moonilal, Morgan Skinner, Noam Miller","doi":"10.1002/cne.70043","DOIUrl":"10.1002/cne.70043","url":null,"abstract":"The effects of environmental enrichment have been well documented in mammals and birds, but less work has focused on reptiles. Because snakes are common in captivity, both as pets and in research/commercial facilities, it is critical to explore how they react to standard captive housing. Here, we examined the effects of environmental enrichment on brain development in a popular pet snake species, the western hognose snake (Heterodon nasicus). Hognose snakes (n = 15) were individually housed for one year in either enriched or standard environments before their brains were harvested and imaged using MRI. We found that enriched snakes had significantly larger brain volumes compared to standard snakes, most prominently in posterior brain regions. In addition, we observed sex-specific brain investments: as snakes grew larger, males displayed relatively larger cerebral hemispheres, and females displayed larger posterior brain regions. These results suggest that environmental enrichment is critical to encouraging healthy brain development in snakes and that snake brain plasticity is very similar to that observed in mammals and birds.","PeriodicalId":15552,"journal":{"name":"Journal of Comparative Neurology","volume":"533 3","pages":""},"PeriodicalIF":2.3,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11926773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0