Journal of Cytology and Histology最新文献

{"title":"Cytomorphologic Evaluation of the Thin Prep for Fine Needle Aspirationof the Fresh Thyroidectomy Specimens","authors":"Yılmaz Baş, H. Havva, E. Keser, H. Behice, E. Erenler","doi":"10.4172/2157-7099.1000445","DOIUrl":"https://doi.org/10.4172/2157-7099.1000445","url":null,"abstract":"Objective: This study aimed to compare cytomorphology of ThinPrep Papanicolaou (TP PAP) method with the macroscopic-microscopic findings of the nodules on fresh thyroidectomy specimens. Study Design: A total of 75 thyroid surgical specimens were included in our study. Fine Needle Aspiration Biopsy (FNAB) of specimens was performed by a pathologist. Aspirate was taken into TP liquid and stained with PAP. We defined cytological findings, compatible with macroscopic-microscopic findings. Results: In cytologic evaluation 108 nodules were diagnosed benign, while 18 nodules were malignant and 2 cystic nodules were insufficient. Cytological diagnosis was compatible with histologically benign a total of 110 nodules, and 18 malignant nodules. Nuclear groove/irregular nuclear membrane, nuclear overlap/crowding, nuclear enlargement, nuclear pseudoinclusion, pale/fine chromatin are determined as statistically significant cytological parameters in malignant cases. Conclusion: FNAB and the histo-morphological and TP PAP cytological slides were evaluated by pathologists at every stage of biopsy process. It is clearly understood how important it is to obtain adequate cells from the benign or malignant thyroid nodules with this method. Although controversial opinions among the cytopathologists regarding t","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75685768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of Mesenchymal Stem Cells vs. Estradiol Benzoate or Avosoya on the Cerebellar Cortex of Ovariectomized Adult Albino Rats","authors":"Samah M Ahmed, Shaimaa A. Abdelrahman, S. Shalaby","doi":"10.4172/2157-7099.1000444","DOIUrl":"https://doi.org/10.4172/2157-7099.1000444","url":null,"abstract":"Alterations in hormone levels during menopause affect cognitive function and brain chemistry. Stem cell therapy in nervous system disorders is a growing field. The present research aimed to compare the effect of MSCs with that of estradiol benzoate or avosoya on the cerebellum of ovariectomized rats. Fifty eight adult female Wistar albino rats were classified into five groups. Control group (Group I) and four ovariectomized groups. Group II (ovariectomized rats received no treatment), Group III (injected subcutaneously with estradiol benzoate), Group IV (received avosoya orally) and Group V (treated with human umbilical cord blood derived mesenchymal stem cells [HUCBMSCs]). Cerebellar sections were stained with hematoxylin and eosin and cresyl fast violet. Immunoperoxidase reaction of glial fibrillary acidic protein (GFAP), estrogen receptor beta (Erβ) and Bax protein was also done. HUCBMSCs homing in rat cerebellum were detected by human albumin gene. Antioxidant enzyme levels were also measured in cerebellum homogenate. Decreased density of neurons in the three cortical layers with vacuolations and distorted Purkinje cells with pyknotic nuclei were observed in Group II. Group III cerebellar cortex showed normal architecture but some Purkinje cells had pyknotic nuclei. Group IV showed normal neuronal cells in the three cortical layers. Group V Purkinje cells were pyriform with vesicular nuclei, and basophilic cytoplasm. Small rounded cells with deeply stained nuclei appeared in the granular layer. In conclusion: estradiol benzoate and avosoya improve ovariectomy-induced cerebellar alteration, and MSCs show promising results in this field.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72947827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological, Histological and Immunohistochemical Study of theRabbit Uterus during Pseudopregnancy","authors":"Abd-Elkareem","doi":"10.4172/2157-7099.1000443","DOIUrl":"https://doi.org/10.4172/2157-7099.1000443","url":null,"abstract":"Ten mature virgin rabbit does (4-5 months old) with mean weight of 2.4 kg were induced to ovulate; by intramuscular injection of HCG (50-70 IU). The day of induction was considered 0 days. Uteri were obtained from 14 h, 3, 7, 18 days post induction. At first stage of pseudopregnancy; the two uteri became hyperemic and slightly swollen. The uterine epithelium and endometrial glands epithelium were of columnar type with oval basal nuclei. The endometrial glands were few in number and small in size. The endometrial glands showed apocrine activity. The uterus had six longitudinal folds and the uterine epithelium form crypts. The endometrium had slight vascularization. At middle stage of pseudopregnancy, the two uteri became hyperemic and more swollen. This stage characterized by; mucosal folding, glandular formation, epithelial proliferation and thickening of the uterine wall. At last stage of pseudopregnancy; the two uteri became flaccid and more swollen. This stage characterized by dramatic changes in the uterine architecture in the form of: increased epithelial proliferation and crypt formation increase the complexity of luminal folding, increase in length, size and abundance of the uterine endometrial glands, increase in the uterine micro vascular development (increased abundance of the large microvessels and development of subepithelial capillary plexuses). Tunica vascularis could be demonstrated between the inner circular and outer longitudinal smooth muscle fibers of the myometrium at the all stages of psedopregnancy. Telocytes with its characteristic morphology could be demonstrated in the myometrium of the rabbit uterus during pseudopregnancy. Cell-specific immuno-localization of progesterone receptors alpha (PRA) in the rabbit uterus during pseudopregnancy revealed that, there were mild, moderate and strong nuclear PRA immunostaining in the endometrial epithelial cells, endometrial glands and in the smooth muscles fibers of the myometrium of the first, middle and last stages of pseudopregnancy respectively. The present study revealed that the morphological, histological and immunohistochemical changes in the rabbit uterus during pseudopregnancy resembled that occurred during early stage of pregnancy. These changes were characterized by dramatic changes in the uterine architecture in the form of: increased epithelial proliferation and crypt formation increase the complexity of luminal folding, increase in length, size and abundance of the uterine endometrial glands, increase in the uterine microvascular development. These uterine changes were accompanied with PRA immunolocalization in the uterine tissues.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"48 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2017-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81000619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell-block in EUS Guided FNA-Best method in a Resource ConstraintSetting?","authors":"N. Gupta","doi":"10.4172/2157-7099.1000447","DOIUrl":"https://doi.org/10.4172/2157-7099.1000447","url":null,"abstract":"Background: Endoscopic ultrasound guided fine needle aspiration (EUS-FNA) has emerged as an effective tool for diagnosing suspected gastro-entero-pancreatic lesions. On site Diff stained smears though have improved the diagnostic accuracy, search have always been made for an effective method of cell block preparation in these cases where diagnosis can further be supplemented by immunohistochemistry (IHC) use.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76466028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural Changes in the Skeletal Muscle Fiber of Adult Male AlbinoRat Following Atorvastatin Treatment; the Possible Mechanisms ofAtorvastatin Induced Myotoxicity","authors":"Sahar Ahmed Mokhmer, E. Saber, Azza A. H. Hamouda, R. Rifaai","doi":"10.4172/2157-7099.1000442","DOIUrl":"https://doi.org/10.4172/2157-7099.1000442","url":null,"abstract":"Method: Rats were divided randomly into four groups; each group included 6 rats: Group I, the control group, received distilled water. Group II received 10 mg/kg/day of atorvastatin. Group III received 40 mg/kg/day of atorvastatin. Group IV received 80 mg/kg/day of atorvastatin. Atorvastatin was given for 8 weeks. Vastus medialis muscle was taken out, rapidly fixed and processed for histological, histochemical and immunohistochemical studies.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83379481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stem Cells, What is Behind?","authors":"Abbas Alnaji","doi":"10.4172/2157-7099.1000476","DOIUrl":"https://doi.org/10.4172/2157-7099.1000476","url":null,"abstract":"","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"55 1","pages":"1-1"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79025206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sharing Virtual Histology Images Worldwide - The Virtual Microscopy Database","authors":"M. Hortsch","doi":"10.4172/2157-7099.100E120","DOIUrl":"https://doi.org/10.4172/2157-7099.100E120","url":null,"abstract":"","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"29 1","pages":"1-2"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84937292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Modulation of Hematological Toxicity during Chemotherapy for Breast Cancer","authors":"Hojouj Mohammad, I. Bondarenko, Zavizion, M. Artemnko, N. Soloviova, Y. Bondarenko, K. Dmytrenko, N. Urzhumova","doi":"10.4172/2157-7099.1000478","DOIUrl":"https://doi.org/10.4172/2157-7099.1000478","url":null,"abstract":"Side effects of anticancer drugs seriously limit the achievement of the maximum therapeutic effect of the most cytostatic. One of the pathophysiological bases of side effects is the ability of cytotoxic agents to intensify the freeradical processes and the consequent lipid peroxidation (LPO) in the cell membranes of various organs. When the antitumor treatment is conducted, the antioxidant enzyme deficiency increases, there is the depletion of nonenzymatic and enzymatic mechanisms of oxidation protection units, resulting in a reduction of organism resistance and damage to vital organs and systems. In this connection it is important to study the possibility of correction of violations occurring in cancer patients with drugs with antioxidant action type.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"267 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76278019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histological Evaluation in Autologous Growth Plate Chondrocyte Grafting in Rabbits","authors":"R. Tomaszewski, A. Gap, Lukasz Wiktor","doi":"10.4172/2157-7099.1000472","DOIUrl":"https://doi.org/10.4172/2157-7099.1000472","url":null,"abstract":"","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81034749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Primary renal myxofibrosarcoma in a woman: A case report and literaturereview","authors":"Sung‐Han Kim, Y. Suh, Jung Kwon Kim, W. Park, Seog‐Yun Park, Ji Hyeon Lee, S. H. Kim, J. Joung, H. Seo, K. Lee, Jinsoo Chung","doi":"10.4172/2157-7099.1000480","DOIUrl":"https://doi.org/10.4172/2157-7099.1000480","url":null,"abstract":"A 45-year-old previously healthy woman presented to our hospital with a right abdominal mass. Abdominal computed tomography (CT), magnetic resonance imaging, and bone scanning revealed a 15-×10-×14-cm, welldelineated, retroperitoneal tumor originating from the right kidney. No metastases detected. The patient underwent successful extirpation of the renal tumor. The tumor showed 50% necrosis without perirenal fat infiltration, and it was diagnosed as a high-grade (grade 3) myxofibrosarcoma without nodal positivity, according to the French Federation of Cancer Centers Sarcoma (FNCLCC) grading system. Tumor immunohistochemical staining revealed positive staining for CD34, Ki67, smooth muscle actin (SMA), and cluster of differentiation 68 (CD68), and negative staining for S100 protein, desmin, mast/stem cell growth factor receptor (c-kit), and melanoma marker antibody (HMB45). Three months post-surgery, follow-up CT revealed no new abdominal metastasis, and the patient is currently receiving routine follow-up without any additional systemic therapy. Myxofibrosarcoma of the kidney is an uncommon soft tissue tumor, and radical surgery is the treatment of choice. Long-term follow-up is recommended because of the tumor’s aggressive invasiveness and potential for distant metastasis.","PeriodicalId":15528,"journal":{"name":"Journal of Cytology and Histology","volume":"35 1","pages":"1-4"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88768704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}