Morphological, Histological and Immunohistochemical Study of theRabbit Uterus during Pseudopregnancy

Abd-Elkareem
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引用次数: 11

Abstract

Ten mature virgin rabbit does (4-5 months old) with mean weight of 2.4 kg were induced to ovulate; by intramuscular injection of HCG (50-70 IU). The day of induction was considered 0 days. Uteri were obtained from 14 h, 3, 7, 18 days post induction. At first stage of pseudopregnancy; the two uteri became hyperemic and slightly swollen. The uterine epithelium and endometrial glands epithelium were of columnar type with oval basal nuclei. The endometrial glands were few in number and small in size. The endometrial glands showed apocrine activity. The uterus had six longitudinal folds and the uterine epithelium form crypts. The endometrium had slight vascularization. At middle stage of pseudopregnancy, the two uteri became hyperemic and more swollen. This stage characterized by; mucosal folding, glandular formation, epithelial proliferation and thickening of the uterine wall. At last stage of pseudopregnancy; the two uteri became flaccid and more swollen. This stage characterized by dramatic changes in the uterine architecture in the form of: increased epithelial proliferation and crypt formation increase the complexity of luminal folding, increase in length, size and abundance of the uterine endometrial glands, increase in the uterine micro vascular development (increased abundance of the large microvessels and development of subepithelial capillary plexuses). Tunica vascularis could be demonstrated between the inner circular and outer longitudinal smooth muscle fibers of the myometrium at the all stages of psedopregnancy. Telocytes with its characteristic morphology could be demonstrated in the myometrium of the rabbit uterus during pseudopregnancy. Cell-specific immuno-localization of progesterone receptors alpha (PRA) in the rabbit uterus during pseudopregnancy revealed that, there were mild, moderate and strong nuclear PRA immunostaining in the endometrial epithelial cells, endometrial glands and in the smooth muscles fibers of the myometrium of the first, middle and last stages of pseudopregnancy respectively. The present study revealed that the morphological, histological and immunohistochemical changes in the rabbit uterus during pseudopregnancy resembled that occurred during early stage of pregnancy. These changes were characterized by dramatic changes in the uterine architecture in the form of: increased epithelial proliferation and crypt formation increase the complexity of luminal folding, increase in length, size and abundance of the uterine endometrial glands, increase in the uterine microvascular development. These uterine changes were accompanied with PRA immunolocalization in the uterine tissues.
兔假妊娠子宫形态、组织学及免疫组织化学研究
10只4 ~ 5月龄、平均体重2.4 kg的成熟处女兔被诱导排卵;肌内注射HCG (50-70 IU)。入职日为0天。诱导后14 h、3、7、18 d取子宫。假妊娠初期;两个子宫充血并轻微肿胀。子宫上皮和子宫内膜腺上皮呈柱状,基底核卵圆形。子宫内膜腺数量少,体积小。子宫内膜腺显示大汗液活性。子宫有6条纵向褶皱,子宫上皮呈隐窝状。子宫内膜有轻微血管化。假孕中期,双子宫充血,肿胀加重。以…为特征的阶段;粘膜折叠,腺体形成,上皮增生和子宫壁增厚。假妊娠末期;两个子宫变得松弛和肿胀。这一阶段以子宫结构的剧烈变化为特征,表现为:上皮增生和隐窝形成增加,管腔折叠的复杂性增加,子宫内膜腺的长度、大小和丰度增加,子宫微血管发育增加(大微血管丰度增加,上皮下毛细血管丛发育)。假妊娠各阶段肌层内、外纵平滑肌纤维间均可见血管层。假妊娠兔子宫肌层中可见具有其特征形态的远端细胞。假妊娠兔子宫中孕激素受体α (PRA)的细胞特异性免疫定位显示,假妊娠前期、中期和后期的子宫内膜上皮细胞、子宫内膜腺体和子宫肌平滑肌纤维中分别出现轻度、中度和强核性PRA免疫染色。本研究发现,兔假孕期间子宫的形态、组织学和免疫组织化学变化与妊娠早期相似。这些变化的特征是子宫结构的剧烈变化,表现为:上皮增生和隐窝形成增加,管腔折叠的复杂性增加,子宫内膜腺体的长度、大小和丰度增加,子宫微血管发育增加。这些子宫变化伴随着子宫组织的PRA免疫定位。
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