Journal of Circadian Rhythms最新文献

Circadian Temperature in Moderate to Severe Acute Stroke Patients. 中度至重度急性中风患者的昼夜温差。

Journal of Circadian Rhythms Pub Date : 2024-08-01 eCollection Date: 2024-01-01 DOI: 10.5334/jcr.241

Jakob Ginsbak Notland, Helle K Iversen, Poul Jennum, Anders S West

{"title":"Circadian Temperature in Moderate to Severe Acute Stroke Patients.","authors":"Jakob Ginsbak Notland, Helle K Iversen, Poul Jennum, Anders S West","doi":"10.5334/jcr.241","DOIUrl":"10.5334/jcr.241","url":null,"abstract":"Background: Stroke patients often present circadian disruption due to multiple causes e.g., primary disease, comorbidities, medication, immobilization, reduced daylight entrainment and sleep disturbances.Objective: To investigate the circadian rhythm of temperature in forehead skin in patients with moderate to severe stroke admitted for rehabilitation.Methods: A physiologic study in form of a secondary analysis of a former randomized study. In total 27 patients with moderate to severe stroke were included between May 1st 2014, and June 1st 2015. Circadian temperature was collected approx. seven days after admission at the acute stroke unit by a skin surface temperature probe as part of a Polysomnography (PSG) measurement.Results: Temperature variations show no circadian rhythm (Type 3 tests of fixed effects by SAS, p = 0.1610). The median temperature variance did fluctuate, but not significantly, and the small changes in circadian temperature variance did not follow the normal temperature variance.Conclusion: Patients with moderate to severe stroke show an abrogated circadian rhythm of temperature. There is an unmet need to understand the mechanisms for this, significance for stroke outcome and treatment.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"22 ","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11295907/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Timely Questions Emerging in Chronobiology: The Circadian Clock Keeps on Ticking 时间生物学中出现的及时问题：昼夜节律钟滴答作响

Journal of Circadian Rhythms Pub Date : 2024-04-02 DOI: 10.5334/jcr.237

Sangeeta Chawla, John S O'Neill, Marina I. Knight, Yuqing He, Lei Wang, Erik Maronde, Sergio Gil Rodríguez, Gerben van Ooijen, Eduardo Garbarino-Pico, Eva Wolf, O. Dkhissi-Benyahya, Anjoom Nikhat, Shaon Chakrabarti, Shawn D. Youngstedt, Natalie Zi-Ching Mak, Ignacio Provencio, Henrik Oster, Namni Goel, Mario Caba, Maria Oosthuizen, Giles E. Duffield, Christopher Chabot, Seth J. Davis

{"title":"Timely Questions Emerging in Chronobiology: The Circadian Clock Keeps on Ticking","authors":"Sangeeta Chawla, John S O'Neill, Marina I. Knight, Yuqing He, Lei Wang, Erik Maronde, Sergio Gil Rodríguez, Gerben van Ooijen, Eduardo Garbarino-Pico, Eva Wolf, O. Dkhissi-Benyahya, Anjoom Nikhat, Shaon Chakrabarti, Shawn D. Youngstedt, Natalie Zi-Ching Mak, Ignacio Provencio, Henrik Oster, Namni Goel, Mario Caba, Maria Oosthuizen, Giles E. Duffield, Christopher Chabot, Seth J. Davis","doi":"10.5334/jcr.237","DOIUrl":"https://doi.org/10.5334/jcr.237","url":null,"abstract":"Chronobiology investigations have revealed much about cellular and physiological clockworks but we are far from having a complete mechanistic understanding of the physiological and ecological implications. Here we present some unresolved questions in circadian biology research as posed by the editorial staff and guest contributors to the Journal of Circadian Rhythms. This collection of ideas is not meant to be comprehensive but does reveal the breadth of our observations on emerging trends in chronobiology and circadian biology. It is amazing what could be achieved with various expected innovations in technologies, techniques, and mathematical tools that are being developed. We fully expect strengthening mechanistic work will be linked to health care and environmental understandings of circadian function. Now that most clock genes are known, linking these to physiological, metabolic, and developmental traits requires investigations from the single molecule to the terrestrial ecological scales. Real answers are expected for these questions over the next decade. Where are the circadian clocks at a cellular level? How are clocks coupled cellularly to generate organism level outcomes? How do communities of circadian organisms rhythmically interact with each other? In what way does the natural genetic variation in populations sculpt community behaviors? How will methods development for circadian research be used in disparate academic and commercial endeavors? These and other questions make it a very exciting time to be working as a chronobiologist.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"28 16","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140754127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reflections on Several Landmark Advances in Circadian Biology 对昼夜节律生物学若干里程碑式进展的思考

Journal of Circadian Rhythms Pub Date : 2024-04-01 DOI: 10.5334/jcr.236

Sangeeta Chawla, Henrik Oster, Giles E. Duffield, Erik Maronde, Mario E Guido, Christopher Chabot, O. Dkhissi-Benyahya, Ignacio Provencio, Namni Goel, Shawn D. Youngstedt, Natalie Zi-Ching Mak, Mario Caba, Anjoom Nikhat, Shaon Chakrabarti, Lei Wang, Seth J. Davis

引用次数: 0

Abnormalities of Rest-Activity and Light Exposure Rhythms Associated with Cognitive Function in Patients with Mild Cognitive Impairment (MCI). 与轻度认知功能障碍（MCI）患者认知功能相关的休息-活动和光照节律异常。

Journal of Circadian Rhythms Pub Date : 2023-12-28 eCollection Date: 2023-01-01 DOI: 10.5334/jcr.227

Seong Jae Kim, Jung Hie Lee, Jae-Won Jang, Hee Seo Jung, In Bum Suh

{"title":"Abnormalities of Rest-Activity and Light Exposure Rhythms Associated with Cognitive Function in Patients with Mild Cognitive Impairment (MCI).","authors":"Seong Jae Kim, Jung Hie Lee, Jae-Won Jang, Hee Seo Jung, In Bum Suh","doi":"10.5334/jcr.227","DOIUrl":"10.5334/jcr.227","url":null,"abstract":"We aimed to examine the difference in rest-activity rhythm (RAR) and light exposure rhythm (LER) between patients with mild cognitive impairment (MCI) and normal controls (NC), and to verify their relationships with cognitive functions. The neuropsychological battery was administered to participants above 50 years old. The MCI diagnosis was made according to Petersen's criteria. Ten patients with MCI (77.90 ± 6.95 years) and eight NC (74.75 ± 5.06 years) were studied. Actigraphy (Actiwatch 2; Philips Respironics) was recorded at home for 5 days. RAR and LER variables, including interdaily stability (IS), intradaily variability (IV) and relative amplitude, were calculated using nonparametric analyses. The associations between cognitive performance and RAR and LER variables were explored using generalized linear models. There were no significant differences in RAR or LER variables between MCI and NC. There was a significant main effect of RAR-IS on the Stroop Color and Word Test (SCWT), indicating a positive relationship between RAR stability and SCWT performance. There was a significant group by RAR-IS interaction on Trail Making Test-A, indicating a negative relationship in MCI compared to NC. There was a significant group by LER-IV interaction on the Boston Naming Test, indicating a positive relationship in MCI compared to NC. There was no disruption in RAR and LER in patients with MCI. Our study showed that circadian rhythm abnormality was associated with a decline in executive function. However, circadian rhythm abnormality was not associated with declines in processing speed and language function in patients with MCI, implying an altered pathophysiology compared to NC.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"21 ","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10756154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139074180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Alarm Clock Against the Sun: Trends in Google Trends Search Activity Across the Transitions to and from Daylight Saving Time. 对抗太阳的闹钟：谷歌趋势搜索活动在夏令时与夏令时之间的过渡趋势。

Journal of Circadian Rhythms Pub Date : 2023-11-29 eCollection Date: 2023-01-01 DOI: 10.5334/jcr.230

Esther Dingena Domenie, Lisa Zarantonello, Chiara Mangini, Chiara Formentin, Gianluca Giusti, Domenico Ruggerini, Paola Cusumano, Alberto Biscontin, Rodolfo Costa, Sara Montagnese

{"title":"The Alarm Clock Against the Sun: Trends in Google Trends Search Activity Across the Transitions to and from Daylight Saving Time.","authors":"Esther Dingena Domenie, Lisa Zarantonello, Chiara Mangini, Chiara Formentin, Gianluca Giusti, Domenico Ruggerini, Paola Cusumano, Alberto Biscontin, Rodolfo Costa, Sara Montagnese","doi":"10.5334/jcr.230","DOIUrl":"https://doi.org/10.5334/jcr.230","url":null,"abstract":"The human circadian timing system depends on the light/dark cycle as its main cue to synchronize with the environment, and thus with solar time. However, human activities depend also on social time, i.e. the set of time conventions and restrictions dictated by society, including Daylight Saving Time (DST), which adds an hour to any degree of desynchrony between social and solar time. Here, we used Google Trends as a data source to analyze diurnal variation, if any, and the daily peak in the relative search volume of 26 Google search queries in relation to the transitions to/from DST in Italy from 2015 to 2020. Our search queries of interest fell into three categories: sleep/health-related, medication and random non sleep/health-related. After initial rhythm and phase analysis, 11 words were selected to compare the average phase of the 15 days before and after the transition to/from DST. We observed an average phase advance after the transition to DST, and a phase delay after the transition to civil time, ranging from 25 to 60 minutes. Advances or delays shorter than 60 minutes, which were primarily observed in the sleep/health-related category, may suggest that search timing for these queries is at least partially driven by the endogenous circadian rhythm. Finally, a significant trend in phase anticipation over the years was observed for virtually all words. This is most likely related to an increase in age, and thus in earlier chronotypes, amongst Google users.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"21 ","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2023-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10705023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138804892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation Analysis Between Time Awareness and Morningness-Eveningness Preference. 时间意识与晨昏偏好的相关性分析。

Journal of Circadian Rhythms Pub Date : 2023-10-11 eCollection Date: 2023-01-01 DOI: 10.5334/jcr.225

Reimi Sogawa, Fuminori Ono, Masahiko Terao, Shunta Nagano, Junko Kawabe, Koichi Node, Makoto Akashi

{"title":"Correlation Analysis Between Time Awareness and Morningness-Eveningness Preference.","authors":"Reimi Sogawa, Fuminori Ono, Masahiko Terao, Shunta Nagano, Junko Kawabe, Koichi Node, Makoto Akashi","doi":"10.5334/jcr.225","DOIUrl":"10.5334/jcr.225","url":null,"abstract":"The circadian clock is adjusted by light inputs via the retinohypothalamic tract. Because environmental light is controllable for modern humans at the individual's preference although under social schedules, individual differences in time-related psychology and behavior may be associated with morningness-eveningness preference (M-E preference). To examine this hypothesis, we used the Time Management Scale and Time Anxiety Scale to quantify time-related psychology and behavior. These scales aim to evaluate \"awareness of effective time management and utilization\" and \"anxiety about uncontrollable time schedule and unexpected time-related outcome\", respectively. According to our correlation analysis using mid-sleep time as a marker for M-E preference, we obtained results supporting our hypothesis in the correlation between the M-E preference values and the Time Management Scale scores, with larger \"time estimation\" and \"taking each moment as it comes\" scores associated with more morningness and eveningness, respectively. Considering that modern humans likely become night owls under artificial light conditions, it appears plausible that lower awareness of time management leads to more eveningness.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"21 ","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41235716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells. 人胚胎肾HEK293细胞内质网质量控制(ERQC)成分的昼夜振荡模式。

Journal of Circadian Rhythms Pub Date : 2023-01-01 DOI: 10.5334/jcr.219

Yalcin Erzurumlu, Deniz Catakli, Hatice Kubra Dogan

{"title":"Circadian Oscillation Pattern of Endoplasmic Reticulum Quality Control (ERQC) Components in Human Embryonic Kidney HEK293 Cells.","authors":"Yalcin Erzurumlu, Deniz Catakli, Hatice Kubra Dogan","doi":"10.5334/jcr.219","DOIUrl":"https://doi.org/10.5334/jcr.219","url":null,"abstract":"The circadian clock regulates the \"push-pull\" of the molecular signaling mechanisms that arrange the rhythmic organization of the physiology to maintain cellular homeostasis. In mammals, molecular clock genes tightly arrange cellular rhythmicity. It has been shown that this circadian clock optimizes various biological processes, including the cell cycle and autophagy. Hence, we explored the dynamic crosstalks between the circadian rhythm and endoplasmic reticulum (ER)-quality control (ERQC) mechanisms. ER-associated degradation (ERAD) is one of the most important parts of the ERQC system and is an elaborate surveillance system that eliminates misfolded proteins. It regulates the steady-state levels of several physiologically crucial proteins, such as 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR) and the metastasis suppressor KAI1/CD82. However, the circadian oscillation of ERQC members and their roles in cellular rhythmicity requires further investigation. In the present study, we provided a thorough investigation of the circadian rhythmicity of the fifteen crucial ERQC members, including gp78, Hrd1, p97/VCP, SVIP, Derlin1, Ufd1, Npl4, EDEM1, OS9, XTP3B, Sel1L, Ufd2, YOD1, VCIP135 and FAM8A1 in HEK293 cells. We found that mRNA and protein accumulation of the ubiquitin conjugation, binding and processing factors, retrotranslocation-dislocation, substrate recognition and targeting components of ERQC exhibit oscillation under the control of the circadian clock. Moreover, we found that Hrd1 and gp78 have a possible regulatory function on Bmal1 turnover. The findings of the current study indicated that the expression level of ERQC components is fine-tuned by the circadian clock and major ERAD E3 ligases, Hrd1 and gp78, may influence the regulation of circadian oscillation by modulation of Bmal1 stability.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"21 ","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10077977/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9277955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Visualization of Age-Dependent Patterns in the Diurnal Transcriptome of Drosophila. 果蝇昼夜转录组中年龄依赖模式的发现与可视化

Journal of Circadian Rhythms Pub Date : 2022-12-08 eCollection Date: 2022-01-01 DOI: 10.5334/jcr.218

Benjamin Sebastian, Rosalyn M Fey, Patrick Morar, Brittany Lasher, Jadwiga M Giebultowicz, David A Hendrix

{"title":"Discovery and Visualization of Age-Dependent Patterns in the Diurnal Transcriptome of Drosophila.","authors":"Benjamin Sebastian, Rosalyn M Fey, Patrick Morar, Brittany Lasher, Jadwiga M Giebultowicz, David A Hendrix","doi":"10.5334/jcr.218","DOIUrl":"10.5334/jcr.218","url":null,"abstract":"Many critical life processes are regulated by input from 24-hour external light/dark cycles, such as metabolism, cellular homeostasis, and detoxification. The circadian clock, which helps coordinate the response to these diurnal light/dark cycles, remains rhythmic across lifespan; however, rhythmic transcript expression is altered during normal aging. To better understand how aging impacts diurnal expression, we present an improved Fourier-based method for detecting and visualizing rhythmicity that is based on the relative power of the 24-hour period compared to other periods (RP24). We apply RP24 to transcript-level expression profiles from the heads of young (5-day) and old (55-day) Drosophila melanogaster, and reveal novel age-dependent rhythmicity changes that may be masked at the gene level. We show that core clock transcripts phase advance during aging, while most rhythmic transcripts phase delay. Transcripts rhythmic only in young flies tend to peak before lights on, while transcripts only rhythmic in old peak after lights on. We show that several pathways, including glutathione metabolism, gain or lose coordinated rhythmic expression with age, providing insight into possible mechanisms of age-onset neurodegeneration. Remarkably, we find that many pathways show very robust coordinated rhythms across lifespan, highlighting their putative roles in promoting neural health. We investigate statistically enriched transcription factor binding site motifs that may be involved in these rhythmicity changes.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"20 ","pages":"1"},"PeriodicalIF":0.0,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9733130/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10423563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery and Visualization of Age-Dependent Patterns in the Diurnal Transcriptome of Drosophila 果蝇日转录组中年龄依赖模式的发现和可视化

Journal of Circadian Rhythms Pub Date : 2022-04-08 DOI: 10.1101/2022.04.06.487011

Benjamin Sebastian, Rosalyn M. Fey, Patrick Morar, Brittany Lasher, Eileen S. Chow, Jadwiga M. Geibultowicz, D. Hendrix

{"title":"Discovery and Visualization of Age-Dependent Patterns in the Diurnal Transcriptome of Drosophila","authors":"Benjamin Sebastian, Rosalyn M. Fey, Patrick Morar, Brittany Lasher, Eileen S. Chow, Jadwiga M. Geibultowicz, D. Hendrix","doi":"10.1101/2022.04.06.487011","DOIUrl":"https://doi.org/10.1101/2022.04.06.487011","url":null,"abstract":"Many critical life processes are regulated by input from 24-hour external light/dark cycles, such as metabolism, cellular homeostasis, and detoxification. The circadian clock, which helps coordinate the response to these diurnal light/dark cycles, remains rhythmic across lifespan; however, rhythmic transcript expression is altered during normal aging. To better understand how aging impacts diurnal expression, we present an improved Fourier-based method for detecting and visualizing rhythmicity that is based on the relative power of the 24-hour period compared to other periods (RP24). We apply RP24 to transcript-level expression profiles from the heads of young (5-day) and old (55-day) Drosophila melanogaster, and reveal novel age-dependent rhythmicity changes that may be masked at the gene level. We show that core clock transcripts phase advance during aging, while most rhythmic transcripts phase delay. Transcripts rhythmic only in young flies tend to peak before lights on, while transcripts only rhythmic in old peak after lights on. We show that several pathways including glutathione metabolism, gain or lose coordinated rhythmic expression with age providing insight into possible mechanisms of age-onset neurodegeneration. Remarkably, we find that many pathways show very robust coordinated rhythms across lifespan, highlighting their putative roles in promoting neural health. We investigate statistically enriched transcription factor binding site motifs that may be involved in these rhythmicity changes.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45535355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord. 大鼠腰脊髓神经递质浓度的昼夜变化

Journal of Circadian Rhythms Pub Date : 2021-07-19 eCollection Date: 2021-01-01 DOI: 10.5334/jcr.215

Beatriz Shantal Jiménez-Zárate, Celia Piña-Leyva, Marina Rodríguez-Sánchez, Benjamín Florán-Garduño, Luis Antonio Jiménez-Zamudio, Ismael Jiménez-Estrada

{"title":"Day-Night Variations in the Concentration of Neurotransmitters in the Rat Lumbar Spinal Cord.","authors":"Beatriz Shantal Jiménez-Zárate, Celia Piña-Leyva, Marina Rodríguez-Sánchez, Benjamín Florán-Garduño, Luis Antonio Jiménez-Zamudio, Ismael Jiménez-Estrada","doi":"10.5334/jcr.215","DOIUrl":"10.5334/jcr.215","url":null,"abstract":"The purpose of this study was to analyze the light-dark variations in the concentrations of several neurotransmitters in the lumbar spinal cord of rats. Six groups of male Wistar rats were exposed to a 12 h light-12 h dark cycle for 70 days. At different time points of the experimental day (8, 12, 16, 20, 24 and 4 h), one of the groups of rats was randomly selected to be sacrificed, and the spinal cords were removed. The gamma-aminobutyric acid (GABA), glutamate (GLU), dopamine, serotonin, epinephrine (E), and norepinephrine (NE) levels in each extracted spinal cord were measured with high-pressure liquid chromatography (HPLC)-EQ and HPLC-fluorescence systems. Our results indicate that the spinal concentrations of GABA and GLU showed sinusoidal variation in a 24 h cycle, with the highest peak in the dark period (~20 h). Dopamine and serotonin also fluctuated in concentration but peaked in the light period (between 8 and 12 h), while E and NE concentrations showed no significant fluctuations. The possible relationship between neurotransmitter spinal concentration and sensitivity to pain and locomotor activity is discussed. It was concluded that most of the neurotransmitter levels in the lumbar spinal cord showed circadian fluctuations coupled to a light-dark cycle.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"19 ","pages":"9"},"PeriodicalIF":0.0,"publicationDate":"2021-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8300578/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39265494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0