Diurnal Variation of 8-hydroxy-2'-deoxyguanosine in Continuous Time Series of Two Breast Cancer Survivors.

Q2 Biochemistry, Genetics and Molecular Biology
Journal of Circadian Rhythms Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI:10.5334/jcr.252
Joschua Geuter, Lennart Seizer, Germaine Cornelissen Guillaume, Ayse Basak Engin, Dietmar Fuchs, Christian Schubert
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引用次数: 0

Abstract

8-hydroxy-2'deoxyguanosine (8-OHdG) is an oxidative product removed from DNA following radical oxygen species-induced damage. As a water-soluble molecule, it can be measured non-invasively in urine and is commonly used as a marker for 'whole-body' oxidative stress. However, its validity and reliability in assessing oxidative stress across various chronic diseases and in early carcinogenesis screening in clinical diagnosis and research remain subjects of debate. To determine optimal measurement timing and duration, it is essential to establish the circadian profile of 8-OHdG under everyday life conditions. Here, applying the integrative single-case design, we show the presence of day-night differences for 8-OHdG in continuous time series of two breast cancer survivors while considering different correction approaches. The participants sampled their urine in 12-h-pooled collections over one month. In both subjects, 8-OHdG levels were significantly higher during the day than at night regardless of whether they were corrected by creatinine or urine volume (creatinine corrected: t = -6.43, p < 0.01 [subject 1], t = -2.69, p = 0.01 [subject 2]; volume corrected: t = -7.30, p < 0.01 [subject 1], t = -3.69, p < 0.01 [subject 2]). Notably, urinary 8-OHdG levels show higher variability in night samples compared to day samples. These findings underscore the importance of considering daily fluctuations in 8-OHdG levels in both clinical and research studies, as well as the need to account for the dynamic characteristics of stress markers to minimize the risk of inconsistent or misleading results in clinical diagnostics.

2例乳腺癌幸存者连续时间序列中8-羟基-2′-脱氧鸟苷的日变化。
8-羟基-2'脱氧鸟苷(8-OHdG)是自由基引起的DNA损伤后的氧化产物。作为一种水溶性分子,它可以在尿液中进行无创测量,通常用作“全身”氧化应激的标记物。然而,它在评估各种慢性疾病的氧化应激以及临床诊断和研究中的早期致癌筛查方面的有效性和可靠性仍然存在争议。为了确定最佳的测量时间和持续时间,有必要在日常生活条件下建立8-OHdG的昼夜节律谱。本研究采用综合单例设计,在考虑不同校正方法的情况下,我们显示了两名乳腺癌幸存者连续时间序列中8-OHdG的昼夜差异。参与者在一个月的时间里抽取了12小时的尿液样本。在两名受试者中,无论是否通过肌酐或尿量校正,8-OHdG水平在白天均显著高于夜间(肌酐校正:t = -6.43, p < 0.01[受试者1],t = -2.69, p = 0.01[受试者2];体积校正:t = -7.30, p < 0.01[受试者1],t = -3.69, p < 0.01[受试者2])。值得注意的是,与白天的样本相比,夜间样本的尿液8-OHdG水平表现出更高的变异性。这些发现强调了在临床和研究中考虑8-OHdG水平每日波动的重要性,以及考虑压力标记物动态特征的必要性,以尽量减少临床诊断结果不一致或误导性的风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Circadian Rhythms
Journal of Circadian Rhythms Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
7.10
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: Journal of Circadian Rhythms is an Open Access, peer-reviewed online journal that publishes research articles dealing with circadian and nycthemeral (daily) rhythms in living organisms, including processes associated with photoperiodism and daily torpor. Journal of Circadian Rhythms aims to include both basic and applied research at any level of biological organization (molecular, cellular, organic, organismal, and populational). Studies of daily rhythms in environmental factors that directly affect circadian rhythms are also pertinent to the journal"s mission.
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