Journal of Circadian Rhythms最新文献_第10页

Journal of Circadian Rhythms: 10th anniversary. 昼夜节律杂志:十周年纪念。

Journal of Circadian Rhythms Pub Date : 2013-01-04 DOI: 10.1186/1740-3391-11-1

Roberto Refinetti

引用次数: 0

Investigation of a non-invasive method of assessing the equine circadian clock using hair follicle cells. 一种利用毛囊细胞评估马生物钟的无创方法的研究。

Journal of Circadian Rhythms Pub Date : 2012-10-05 DOI: 10.1186/1740-3391-10-7

Lisa M Watts, John A Browne, Barbara A Murphy

{"title":"Investigation of a non-invasive method of assessing the equine circadian clock using hair follicle cells.","authors":"Lisa M Watts, John A Browne, Barbara A Murphy","doi":"10.1186/1740-3391-10-7","DOIUrl":"https://doi.org/10.1186/1740-3391-10-7","url":null,"abstract":"Unlabelled: Background: A comprehensive understanding of the equine circadian clock involves the evaluation of circadian clock gene expression. A non-invasive and effective method for detecting equine clock gene expression has yet to be established. Currently, research surrounding this area has relied on collecting tissue biopsies or blood samples that can often be costly, time consuming and uncomfortable for the animal.Methods: Five mares were individually stabled under a light-dark (LD) cycle that mimicked the external environmental photoperiod during a time of year corresponding with the vernal equinox. Hair follicles were collected every 4 h over a 24-h period by plucking hairs from the mane. RNA was extracted and quantitative (q) PCR assays were performed to determine temporal expression patterns for the core clock genes; ARNTL, CRY1, PER1, PER2, NR1D2 and the clock controlled gene, DBP.Results: Repeated measures ANOVA for the clock gene transcripts PER1 and PER2 and the clock controlled gene, DBP, revealed significant variation in expression over time (p < .05, respectively). Cosinor analysis confirmed a significant 24-h temporal component for PER1 (p = .002) and DBP (p = .0033) and also detected rhythmicity for NR1D2 (p = .0331).Conclusions: We show that the extraction of RNA from equine hair follicle cells can identify the circadian 24 h oscillations of specific clock genes and a clock-controlled gene and therefore provide a valuable non-invasive method for evaluating the equine peripheral circadian clock. This method will serve as a useful tool for future evaluations of equine circadian rhythms and their response to environmental changes.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"10 1","pages":"7"},"PeriodicalIF":0.0,"publicationDate":"2012-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1740-3391-10-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30957183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

The effects of lighting conditions and food restriction paradigms on locomotor activity of common spiny mice, Acomys cahirinus. 光照条件和食物限制范式对普通刺鼠运动活动的影响

Journal of Circadian Rhythms Pub Date : 2012-09-09 DOI: 10.1186/1740-3391-10-6

Christopher C Chabot, Devin M Connolly, Brenda B Waring

{"title":"The effects of lighting conditions and food restriction paradigms on locomotor activity of common spiny mice, Acomys cahirinus.","authors":"Christopher C Chabot, Devin M Connolly, Brenda B Waring","doi":"10.1186/1740-3391-10-6","DOIUrl":"10.1186/1740-3391-10-6","url":null,"abstract":"Background: An endogenous circadian clock controls locomotor activity in common spiny mice (Acomys cahirinus). However, little is known about the effects of constant light (LL) on this activity or about the existence of an additional food entrainable clock. A series of experiments were performed to investigate the effects of LL and DD on tau and activity levels.Methods: Spiny mice were housed individually and their running wheel activity monitored. One group of mice was exposed to LD, DD and several intensities of LL. Another group was exposed to a restricted feeding (RF) paradigm in light: dark (LD) during one hour before the L to D transition. Significance of rhythmicity was assessed using Lomb-Scargle periodograms.Results: In LD all animals exhibited nocturnal activity rhythms that persisted in DD. When animals were exposed to RF (during L), all of these animals (n = 11) demonstrated significant food anticipatory activity as well as an increase in diurnal activity. This increase in diurnal activity persisted in 4/11 animals during subsequent ad libitum conditions. Under LL conditions, the locomotor rhythms of 2/11 animals appeared to entrain to RF. When animals were exposed to sequentially increasing LL intensities, rhythmicity persisted and, while activity decreased significantly, the free-running period was relatively unaffected. In addition, the period in LL was significantly longer than the period in DD. Exposure to LL also induced long-term changes (after-effects) on period and activity when animals were again exposed to DD.Conclusions: Overall these studies demonstrate clear and robust circadian rhythms of wheel-running in A. cahirinus. In addition, LL clearly inhibited activity in this species and induced after-effects. The results also confirm the presence of a food entrainable oscillator in this species.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"10 1","pages":"6"},"PeriodicalIF":0.0,"publicationDate":"2012-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3485179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30889154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diurnal-and sex-related difference of metallothionein expression in mice. 小鼠金属硫蛋白表达的昼夜差异和性别差异

Journal of Circadian Rhythms Pub Date : 2012-07-24 DOI: 10.1186/1740-3391-10-5

Dan Zhang, Tao Jin, Yi-Qiao Xu, Yuan-Fu Lu, Qin Wu, Yu-Kun Jennifer Zhang, Jie Liu

{"title":"Diurnal-and sex-related difference of metallothionein expression in mice.","authors":"Dan Zhang, Tao Jin, Yi-Qiao Xu, Yuan-Fu Lu, Qin Wu, Yu-Kun Jennifer Zhang, Jie Liu","doi":"10.1186/1740-3391-10-5","DOIUrl":"10.1186/1740-3391-10-5","url":null,"abstract":"Background: Metallothionein (MT) is a small, cysteine-rich, metal-binding protein that plays an important role in protecting against toxicity of heavy metal and chemicals. This study was aimed to define diurnal and sex variation of MT in mice.Methods: Adult mice were maintained in light- and temperature-controlled facilities for 2 weeks with light on at 8:00 and light off at 20:00. The blood, liver, and kidneys were collected every 4 h during the 24 h period. Total RNA was isolated, purified, and subjected to real-time RT-PCR analysis and MT protein was determined by western blot and the Cd/hemoglobin assay.Results: The diurnal variations in mRNA levels of MT-1 and MT-2in liver were dramatic, up to a 40-foldpeak/trough ratio. MT mRNA levels in kidneys and blood also showed diurnal variation, up to 5-fold peak/trough ratio. The diurnal variation of MT mRNAs resembled the clock gene albumin site D-binding protein (Dbp), and was anti-phase to the clock gene Brain and Muscle ARNT-like Protein 1 (Bmal1) in liver and kidneys. The peaks of MT mRNA levels were higher in females than in males. Hepatic MT protein followed a similar pattern, with about a 3-fold difference.Conclusion: MT mRNA levels and protein showed diurnal- and sex-variation in liver, kidney, and blood of mice, which could impact the body defense against toxic stimuli.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"10 1","pages":"5"},"PeriodicalIF":0.0,"publicationDate":"2012-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3585924/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30786756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential roles of breakfast only (one meal per day) and a bigger breakfast with a small dinner (two meals per day) in mice fed a high-fat diet with regard to induced obesity and lipid metabolism. 在高脂肪饮食小鼠中，只吃早餐(每天一餐)和早餐多吃晚餐少(每天两餐)对诱导肥胖和脂质代谢的不同作用。

Journal of Circadian Rhythms Pub Date : 2012-05-15 DOI: 10.1186/1740-3391-10-4

Yuta Fuse, Akiko Hirao, Hiroaki Kuroda, Makiko Otsuka, Yu Tahara, Shigenobu Shibata

{"title":"Differential roles of breakfast only (one meal per day) and a bigger breakfast with a small dinner (two meals per day) in mice fed a high-fat diet with regard to induced obesity and lipid metabolism.","authors":"Yuta Fuse, Akiko Hirao, Hiroaki Kuroda, Makiko Otsuka, Yu Tahara, Shigenobu Shibata","doi":"10.1186/1740-3391-10-4","DOIUrl":"https://doi.org/10.1186/1740-3391-10-4","url":null,"abstract":"Background: Recent studies on humans and rodents have suggested that the timing of food intake plays an important role in circadian regulation and metabolic health. Consumption of high-fat foods during the inactive period or at the end of the awake period results in weight gain and metabolic syndrome in rodents. However, the distinct effects of breakfast size and the breakfast/dinner size ratio on metabolic health have not yet been fully examined in mice.Methods: We examined whether the parameters of metabolic syndrome were differentially affected in mice that consumed a large meal at the beginning of the awake period (breakfast; one meal group) and a relatively smaller meal at end of the awake period (dinner; two meals group). The mice of each group were provided equal food volume per day.Results: Mice on one meal exhibited an increase in body weight gain, hyperinsulinemia, hyperleptinemia, and a decrease of gene expression associated with β-oxidation in adipose tissue and liver compared with those on two meals. The circadian expression pattern of the Clock gene in mice on one meal was disturbed compared with those on two meals.Conclusions: In conclusion, a bigger breakfast with a smaller dinner (two meals per day) but not breakfast only (one meal per day) helps control body weight and fat accumulation in mice on a high-fat meals schedule. The findings of this study suggest that dietary recommendations for weight reduction and/or maintenance should include information on the timing and quantity of dietary intake.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"10 1","pages":"4"},"PeriodicalIF":0.0,"publicationDate":"2012-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1740-3391-10-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30618530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 64

Chronotolerance study of the antiepileptic drug valproic acid in mice. 抗癫痫药丙戊酸小鼠时间耐受性研究。

Journal of Circadian Rhythms Pub Date : 2012-05-10 DOI: 10.1186/1740-3391-10-3

Wafa Ben-Cherif, Ichrak Dridi, Karim Aouam, Mossadok Ben-Attia, Alain Reinberg, Naceur A Boughattas

{"title":"Chronotolerance study of the antiepileptic drug valproic acid in mice.","authors":"Wafa Ben-Cherif, Ichrak Dridi, Karim Aouam, Mossadok Ben-Attia, Alain Reinberg, Naceur A Boughattas","doi":"10.1186/1740-3391-10-3","DOIUrl":"https://doi.org/10.1186/1740-3391-10-3","url":null,"abstract":"Background: Valproic acid (VPA) is an antiepileptic drug widely used for the treatment of absence seizures and generalized tonic-clonic seizures. The present work aims to study whether VPA-induced toxicity varies according to the dosing-time in the 24 hour-scale.Methods: The influence of dosing-time on tolerance to VPA was investigated in 120 male Swiss mice synchronized under a light-dark cycle (12:12). The mean VPA lethal dose was first determined to be 850 ± 0.2 mg/kg, i.p.. Such a dose was administered by i.p. route to a total of 90 mice divided in six circadian stages [1, 5, 9, 13, 17 and 21 Hours After Light Onset (HALO)] (15 mice/circadian time); 30 mice were used as control (5 mice / circadian time).Results: The surviving treated mice exhibited a significant circadian variation in rectal temperature and body weight loss (p < 0.001). The least rectal temperature change and body weight loss occurred when VPA was injected at 9 HALO. Drug dosing at 9 HALO resulted in -9 % weight loss whereas drug dosing at 17 HALO was -15 % (Ø = 20.3 HALO ± 1.1 h, p ≤ 0.0001). Lethal toxicity also varied according to circadian dosing-time (χ2 = 42.1, p < 0.0001). The highest (60 %) and the lowest (6.67 %) survival rates were observed at 9 HALO and 17 HALO respectively. Cosinor analyses validated a significant circadian rhythm in survival duration with an acrophase at 8.4 HALO ± 0.75 h (p < 0.001).Conclusions: With regards to these data the optimal tolerance to VPA occurred when the drug was administered in the second half of the light-rest span of mice which is physiologically analogous to the second half of the night for human patients.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"10 ","pages":"3"},"PeriodicalIF":0.0,"publicationDate":"2012-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1740-3391-10-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30609506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Summer activity patterns among teenage girls: harmonic shape invariant modeling to estimate circadian cycles. 少女的夏季活动模式：用谐波形状不变模型估算昼夜节律周期。

Journal of Circadian Rhythms Pub Date : 2012-05-06 DOI: 10.1186/1740-3391-10-2

Semhar Ogbagaber, Paul S Albert, Daniel Lewin, Ronald J Iannotti

{"title":"Summer activity patterns among teenage girls: harmonic shape invariant modeling to estimate circadian cycles.","authors":"Semhar Ogbagaber, Paul S Albert, Daniel Lewin, Ronald J Iannotti","doi":"10.1186/1740-3391-10-2","DOIUrl":"10.1186/1740-3391-10-2","url":null,"abstract":"Background: Physical activity as measured by activity counts over short time intervals across a 24 h period are often used to assess circadian variation. We are interested in characterizing circadian patterns in activity among adolescents and examining how these patterns vary by obesity status. New statistical approaches are needed to examine how factors affect different features of the circadian pattern and to make appropriate covariate adjustments when the outcomes are longitudinal count data.Methods: We develop a statistical model for longitudinal or repeated activity count data that is used to examine differences in the overall activity level, amplitude (defined as the difference between the lowest and highest activity level over a 24 hour period), and phase shift. Using seven days of continuous activity monitoring, we characterize the circadian patterns and compare them between obese and non-obese adolescent girls.Results: We find a statistically significant phase delay in adolescent girls who were obese compared with their non-obese counterparts. After the appropriate adjustment for measured potential confounders, we did not find differences in mean activity level between the two groups.Conclusion: New statistical methodology was developed to identify a phase delay in obese compared with non-obese adolescents. This new approach for analyzing longitudinal circadian rhythm count data provides a useful statistical technique to add to the repertoire for those analyzing circadian rhythm data.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"10 1","pages":"2"},"PeriodicalIF":0.0,"publicationDate":"2012-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3464928/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30595343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A dynamic model of circadian rhythms in rodent tail skin temperature for comparison of drug effects. 鼠尾皮肤温度昼夜节律的动态模型，用于药物效果的比较。

Journal of Circadian Rhythms Pub Date : 2012-01-05 DOI: 10.1186/1740-3391-10-1

Dorothee Girbig, Karsten Keller, Katja Prelle, Vladimir Patchev, Richardus Vonk, Bernd-Wolfgang Igl

引用次数: 15

Magel2, a Prader-Willi syndrome candidate gene, modulates the activities of circadian rhythm proteins in cultured cells. Magel2是一种Prader-Willi综合征候选基因，可调节培养细胞中昼夜节律蛋白的活动。

Journal of Circadian Rhythms Pub Date : 2011-12-30 DOI: 10.1186/1740-3391-9-12

Julia Devos, Sara V Weselake, Rachel Wevrick

{"title":"Magel2, a Prader-Willi syndrome candidate gene, modulates the activities of circadian rhythm proteins in cultured cells.","authors":"Julia Devos, Sara V Weselake, Rachel Wevrick","doi":"10.1186/1740-3391-9-12","DOIUrl":"https://doi.org/10.1186/1740-3391-9-12","url":null,"abstract":"Background: The Magel2 gene is most highly expressed in the suprachiasmatic nucleus of the hypothalamus, where its expression cycles in a circadian pattern comparable to that of clock-controlled genes. Mice lacking the Magel2 gene have hypothalamic dysfunction, including circadian defects that include reduced and fragmented total activity, excessive activity during the subjective day, but they have a normal circadian period. Magel2 is a member of the MAGE family of proteins that have various roles in cellular function, but the specific function of Magel2 is unknown.Methods: We used a variety of cell-based assays to determine whether Magel2 modifies the properties of core circadian rhythm proteins.Results: Magel2 represses the activity of the Clock:Bmal1 heterodimer in a Per2-luciferase assay. Magel2 interacts with Bmal1 and with Per2 as measured by co-immunoprecipitation in co-transfected cells, and exhibits a subcellular distribution consistent with these interactions when visualized by immunofluorescence. As well, Magel2 induces the redistribution of the subcellular localization of Clock towards the cytoplasm, in contrast to the nucleus-directed effect of Bmal1 on Clock subcellular localization.Conclusion: Consistent with the blunted circadian rhythm observed in Magel2-null mice, these data suggest that Magel2 normally promotes negative feedback regulation of the cellular circadian cycle, through interactions with key core circadian rhythm proteins.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"9 1","pages":"12"},"PeriodicalIF":0.0,"publicationDate":"2011-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1740-3391-9-12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30356692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Measuring the impact of apnea and obesity on circadian activity patterns using functional linear modeling of actigraphy data. 使用活动记录仪数据的功能线性建模来测量呼吸暂停和肥胖对昼夜活动模式的影响。

Journal of Circadian Rhythms Pub Date : 2011-10-13 DOI: 10.1186/1740-3391-9-11

Jia Wang, Hong Xian, Amy Licis, Elena Deych, Jimin Ding, Jennifer McLeland, Cristina Toedebusch, Tao Li, Stephen Duntley, William Shannon

{"title":"Measuring the impact of apnea and obesity on circadian activity patterns using functional linear modeling of actigraphy data.","authors":"Jia Wang, Hong Xian, Amy Licis, Elena Deych, Jimin Ding, Jennifer McLeland, Cristina Toedebusch, Tao Li, Stephen Duntley, William Shannon","doi":"10.1186/1740-3391-9-11","DOIUrl":"https://doi.org/10.1186/1740-3391-9-11","url":null,"abstract":"Background: Actigraphy provides a way to objectively measure activity in human subjects. This paper describes a novel family of statistical methods that can be used to analyze this data in a more comprehensive way.Methods: A statistical method for testing differences in activity patterns measured by actigraphy across subgroups using functional data analysis is described. For illustration this method is used to statistically assess the impact of apnea-hypopnea index (apnea) and body mass index (BMI) on circadian activity patterns measured using actigraphy in 395 participants from 18 to 80 years old, referred to the Washington University Sleep Medicine Center for general sleep medicine care. Mathematical descriptions of the methods and results from their application to real data are presented.Results: Activity patterns were recorded by an Actical device (Philips Respironics Inc.) every minute for at least seven days. Functional linear modeling was used to detect the association between circadian activity patterns and apnea and BMI. Results indicate that participants in high apnea group have statistically lower activity during the day, and that BMI in our study population does not significantly impact circadian patterns.Conclusions: Compared with analysis using summary measures (e.g., average activity over 24 hours, total sleep time), Functional Data Analysis (FDA) is a novel statistical framework that more efficiently analyzes information from actigraphy data. FDA has the potential to reposition the focus of actigraphy data from general sleep assessment to rigorous analyses of circadian activity rhythms.","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"9 1","pages":"11"},"PeriodicalIF":0.0,"publicationDate":"2011-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1186/1740-3391-9-11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30060062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7