Voluntary Running and Estrous Cycle Modulate ΔFOSB in the Suprachiasmatic Nucleus of the Wistar Rat.

Q2 Biochemistry, Genetics and Molecular Biology
Journal of Circadian Rhythms Pub Date : 2025-05-19 eCollection Date: 2025-01-01 DOI:10.5334/jcr.257
Ayano Shiba, Marene H Hardonk, Ewout Foppen, Tess Kool, Susanne E la Fleur, Paul J Lucassen, Chun-Xia Yi, Dirk Jan Stenvers, Joram D Mul, Andries Kalsbeek
{"title":"Voluntary Running and Estrous Cycle Modulate ΔFOSB in the Suprachiasmatic Nucleus of the Wistar Rat.","authors":"Ayano Shiba, Marene H Hardonk, Ewout Foppen, Tess Kool, Susanne E la Fleur, Paul J Lucassen, Chun-Xia Yi, Dirk Jan Stenvers, Joram D Mul, Andries Kalsbeek","doi":"10.5334/jcr.257","DOIUrl":null,"url":null,"abstract":"<p><p>The hypothalamic suprachiasmatic nucleus (SCN), the circadian pacemaker of the mammalian brain, integrates both environmental and endogenous information to modulate various physiological and behavioral processes. Both light and physical activity entrain SCN circadian rhythmicity, but the underlying molecular mechanisms for physical activity remain elusive. Repetitive neuronal stimulation results in accumulation of the stable transcription factor ΔFOSB, that has been implicated in long-term brain plasticity, altered neuronal excitability, and changes in behavior. In rodents, voluntary wheel running (VWR) mimics aspects of exercise training and increases ΔFOSB in several brain regions. Whether VWR also alters ΔFOSB in the SCN is unexplored. Here, young-adult male and female Wistar rats were housed sedentary or allowed to run for four weeks followed by quantification of ΔFOSB in the SCN. VWR lowered SCN ΔFOSB-positive cell numbers in males and females compared to sedentary housing. Total running distance did not correlate with ΔFOSB suppression. Analysis taking estrous cycle into account revealed that ΔFOSB-positive cell numbers were cyclic in sedentary females, being lowest during proestrus and highest during diestrus. Remarkably, this cyclicity was absent in runners, where ΔFOSB-positive cell numbers remained comparable to those observed during proestrus in sedentary controls. Finally, estradiol replacement following ovariectomy in sedentary females lowered SCN ΔFOSB-positive cell numbers. Thus, VWR and estrous cycle, via, at least in part, estradiol, modulate SCN ΔFOSB. Given its role in long-term plasticity and behavioral adaptations, ΔFOSB may provide a molecular link between VWR and/or estrous cycle and the output of the SCN and its related behavioral adaptations.</p>","PeriodicalId":15461,"journal":{"name":"Journal of Circadian Rhythms","volume":"23 ","pages":"7"},"PeriodicalIF":0.0000,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12101111/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Circadian Rhythms","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5334/jcr.257","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}
引用次数: 0

Abstract

The hypothalamic suprachiasmatic nucleus (SCN), the circadian pacemaker of the mammalian brain, integrates both environmental and endogenous information to modulate various physiological and behavioral processes. Both light and physical activity entrain SCN circadian rhythmicity, but the underlying molecular mechanisms for physical activity remain elusive. Repetitive neuronal stimulation results in accumulation of the stable transcription factor ΔFOSB, that has been implicated in long-term brain plasticity, altered neuronal excitability, and changes in behavior. In rodents, voluntary wheel running (VWR) mimics aspects of exercise training and increases ΔFOSB in several brain regions. Whether VWR also alters ΔFOSB in the SCN is unexplored. Here, young-adult male and female Wistar rats were housed sedentary or allowed to run for four weeks followed by quantification of ΔFOSB in the SCN. VWR lowered SCN ΔFOSB-positive cell numbers in males and females compared to sedentary housing. Total running distance did not correlate with ΔFOSB suppression. Analysis taking estrous cycle into account revealed that ΔFOSB-positive cell numbers were cyclic in sedentary females, being lowest during proestrus and highest during diestrus. Remarkably, this cyclicity was absent in runners, where ΔFOSB-positive cell numbers remained comparable to those observed during proestrus in sedentary controls. Finally, estradiol replacement following ovariectomy in sedentary females lowered SCN ΔFOSB-positive cell numbers. Thus, VWR and estrous cycle, via, at least in part, estradiol, modulate SCN ΔFOSB. Given its role in long-term plasticity and behavioral adaptations, ΔFOSB may provide a molecular link between VWR and/or estrous cycle and the output of the SCN and its related behavioral adaptations.

Wistar大鼠视交叉上核自主跑步和发情周期调节ΔFOSB。
下丘脑视交叉上核(SCN)是哺乳动物大脑的昼夜节律起搏器,整合环境和内源性信息来调节各种生理和行为过程。光照和体力活动都会影响SCN的昼夜节律,但体力活动的潜在分子机制仍然难以捉摸。重复的神经元刺激导致稳定转录因子ΔFOSB的积累,这与长期的大脑可塑性、神经元兴奋性的改变和行为的改变有关。在啮齿类动物中,自愿轮跑(VWR)模仿了运动训练的各个方面,并增加了大脑几个区域的ΔFOSB。VWR是否也会改变SCN中的ΔFOSB还没有研究。在这里,年轻的成年雄性和雌性Wistar大鼠被安置在静止或允许跑步四周,然后定量SCN中的ΔFOSB。与久坐的住房相比,VWR降低了男性和女性的SCN ΔFOSB-positive细胞数量。总跑步距离与ΔFOSB抑制无关。考虑到发情周期的分析显示,久坐不动的女性的ΔFOSB-positive细胞数量是周期性的,在发情前期最低,在发情期间最高。值得注意的是,这种循环在跑步者中不存在,ΔFOSB-positive细胞数量与久坐对照组在发情前观察到的细胞数量相当。最后,久坐女性卵巢切除术后的雌二醇替代降低了SCN ΔFOSB-positive细胞数量。因此,VWR和发情周期,至少部分通过雌二醇调节SCN ΔFOSB。鉴于其在长期可塑性和行为适应中的作用,ΔFOSB可能提供了VWR和/或发情周期与SCN输出及其相关行为适应之间的分子联系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Circadian Rhythms
Journal of Circadian Rhythms Biochemistry, Genetics and Molecular Biology-Physiology
CiteScore
7.10
自引率
0.00%
发文量
0
审稿时长
12 weeks
期刊介绍: Journal of Circadian Rhythms is an Open Access, peer-reviewed online journal that publishes research articles dealing with circadian and nycthemeral (daily) rhythms in living organisms, including processes associated with photoperiodism and daily torpor. Journal of Circadian Rhythms aims to include both basic and applied research at any level of biological organization (molecular, cellular, organic, organismal, and populational). Studies of daily rhythms in environmental factors that directly affect circadian rhythms are also pertinent to the journal"s mission.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信