Journal of Clinical Periodontology最新文献

{"title":"HIF-1α/HO-1 Axis Promotes Ferroptosis and Impairs Periodontal Tissue Repair in Periodontitis","authors":"Xiaochi Chang,&nbsp;Jie Zhang,&nbsp;Ye Han,&nbsp;Yang Wang,&nbsp;Cheng Lin,&nbsp;Chang Shu,&nbsp;Yanghao Zhao,&nbsp;Yi Feng,&nbsp;Yan Han,&nbsp;Jianxia Hou","doi":"10.1111/jcpe.70150","DOIUrl":"10.1111/jcpe.70150","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate whether the hypoxia-inducible factor-1alpha (HIF-1α)/heme oxygenase-1 (HO-1) axis mediates ferroptosis in periodontal ligament tissues and contributes to impaired periodontal tissue repair in periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>RNA sequencing was performed to identify ferroptosis-related pathways in ligature-induced periodontitis (LIP) model. In vivo, LIP and ligature-removal recovery models were treated with the ferroptosis inhibitor Ferrostatin-1 (Fer-1). Alveolar bone changes were assessed by micro-computed tomography. Osteoclast activity and periodontal ligament–associated repair were evaluated by TRAP staining and Periostin immunohistochemistry. Ferroptosis-related alterations were assessed by immunohistochemistry of HIF-1α, HO-1, GPX4, SLC7A11, 4-HNE and Perls staining. In vitro, an HIF-1α-activated hPDLSC model was established. Ferroptosis-related changes were evaluated by ROS, MDA, Fe²⁺ levels, C11-BODIPY staining and expression of ferroptosis-associated molecules. The HIF-1α/HO-1 regulatory relationship was examined by ChIP-qPCR and dual-luciferase reporter assays.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>RNA sequencing revealed enrichment of ferroptosis-related pathways and identified HIF-1α and HO-1 as central regulators in inflamed periodontal ligament tissues. In vivo, Fer-1 attenuated alveolar bone loss, osteoclast activity and inflammatory cell infiltration. These changes were accompanied by decreased HO-1 expression, iron deposition and 4-HNE levels, along with increased GPX4 and SLC7A11 expression. In the recovery model, Fer-1 further reduced oxidative stress, suppressed osteoclast activity and increased Periostin expression, indicating improved periodontal tissue repair. In vitro, HIF-1α activation was associated with increased Fe²⁺ accumulation, ROS production and ferroptosis-related molecular alterations. Mechanistically, HIF-1α directly transactivated HO-1, which contributed to iron accumulation and downstream ferroptosis-associated changes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>The HIF-1α/HO-1 signalling axis is associated with ferroptosis-related alterations in the PDL, which constitute periodontal tissue damage and impaired repair in periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 8","pages":"1364-1377"},"PeriodicalIF":8.3,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148205178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stage-Associated Subgingival Dysbiosis Across the Periodontal Disease Spectrum in a Thai Cohort","authors":"Teerat Sawangpanyangkura,&nbsp;Kanokphong Suparan,&nbsp;Chanyanuch Wechwithayakhlung,&nbsp;Chanon Kunasol,&nbsp;Nipon Chattipakorn,&nbsp;Siriporn C. Chattipakorn","doi":"10.1111/jcpe.70147","DOIUrl":"10.1111/jcpe.70147","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aims</h3>\u0000 \u0000 <p>This cross-sectional study aimed to investigate the subgingival microbiota and predict functional profiles across the 2018 EFP/AAP periodontal disease spectrum in a Thai clinical cohort.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Subgingival plaque from 100 participants (gingivitis, <i>n</i> = 30; Stage II–IV periodontitis, <i>n</i> = 70) was analysed by 16S rRNA gene sequencing with taxonomic assignment using the Human Oral Microbiome Database.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p><i>Peptostreptococcaceae</i>-related taxa, <i>Dialister pneumosintes</i>, <i>Shuttleworthia satelles</i>, <i>Prevotella</i> sp. HMT-304, and Bacteroidetes [G-3] bacterium HMT-280 were recurrently identified across differential abundance and penalised regression analyses. Penalised regression models showed limited stage-discriminative performance (multinomial AUC 0.68–0.78), with gingivitis achieving the highest classification accuracy (63%) and Stage IV the lowest, consistent with overlapping microbial profiles across stages. Nonetheless, some stage-enriched taxa included <i>Campylobacter</i> species in Stage III and <i>Porphyromonas gingivalis</i> with <i>Odoribacter</i> bacterium HMT-516 in Stage IV. Stage-enriched taxa correlated with anaerobic fermentation pathways, while co-occurrence networks became less dense but broader across stages.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Stage-associated microbiome shifts were detectable using a gingivitis reference group, with a disease-associated core and accompanying anaerobic functional enrichment. The findings suggest that subgingival dysbiosis across periodontitis stages does not follow a simple linear trajectory and that clinical stage is not determined by microbiome composition alone.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 8","pages":"1332-1343"},"PeriodicalIF":8.3,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148042345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontitis and Endotoxemia: Clinical and Microbiological Insights","authors":"Anbo Dong,&nbsp;Muhammed Manzoor,&nbsp;Jaakko Leskelä,&nbsp;Jukka Putaala,&nbsp;Eija Könönen,&nbsp;Yi Jin,&nbsp;Pirkko Pussinen,&nbsp;Susanna Paju,&nbsp;Svetislav Zaric","doi":"10.1111/jcpe.70148","DOIUrl":"10.1111/jcpe.70148","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>Endotoxemia has been proposed as a link between periodontitis and systemic complications through the translocation of lipopolysaccharide (LPS) from inflamed periodontal sites into the bloodstream. This study aimed to investigate subgingival and serum LPS activities and their correlations with periodontal clinical and microbiological parameters.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Two-hundred and seventy participants with gingivitis and periodontitis from the SECRETO Oral cohort were included in the study. Serum and subgingival plaque samples were analysed for LPS activity using the recombinant Factor C assay, and subgingival microbiota were profiled by 16S rRNA sequencing. Dysbiosis indices and taxonomic associations were assessed through multivariate regressions and MaAsLin2 models adjusted for clinical and demographic covariates. Functional pathways were inferred using PICRUSt2.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Serum LPS activities were significantly elevated in periodontitis patients compared to gingivitis patients and correlated with the subgingival microbial dysbiosis index (SMDI). Subgingival LPS was also significantly higher in periodontitis patients and showed strong associations with subgingival bacterial load and SMDI. Subgingival LPS explained 10% of serum LPS variations. Differential abundance analysis identified <i>Fretibacterium</i> as positively associated with both subgingival and serum LPS activities. Functional prediction revealed enrichment of bacterial motility and invasion pathways, with depletion of host defence functions, supporting a mechanistic link between subgingival dysbiosis, LPS translocation and systemic endotoxemia.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>These findings provide novel evidence that elevated serum LPS activities are closely linked with periodontitis diagnosis, associated with subgingival dysbiosis, and highlight the periodontal niche as a source of systemic endotoxemia. In addition, it identified <i>Fretibacterium</i> as the main contributor to both subgingival and serum LPS activities.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 8","pages":"1344-1354"},"PeriodicalIF":8.3,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70148","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Fasting-Mimicking Diet Affects the Inflammatory Response Following Periodontal Treatment: A Multi-centre Feasibility Randomised Controlled Pilot Trial","authors":"Giuseppe Mainas,&nbsp;Elena Figuero,&nbsp;Marta Amigo Basilio,&nbsp;José Dopico,&nbsp;Florencia Julieta Gayo Morales,&nbsp;Antonio Magan-Fernandez,&nbsp;Inmaculada Cabello,&nbsp;Guillermo Pardo Zamora,&nbsp;Josefina Guillén Sanchez,&nbsp;Jose Nart,&nbsp;Antonio Santos Alemany,&nbsp;Carlos Pereira Couto,&nbsp;Manlio Vinciguerra,&nbsp;Valter D. Longo,&nbsp;Mark Ide,&nbsp;Mariano Sanz,&nbsp;Luigi Nibali","doi":"10.1111/jcpe.70139","DOIUrl":"10.1111/jcpe.70139","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate whether cycles of fasting-mimicking diet (FMD), used as an adjunct to non-surgical periodontal treatment, are feasible and could influence clinical and inflammatory responses, particularly with respect to C-reactive protein (CRP).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Individuals with periodontitis were randomised to receive steps 1 and 2 of periodontal treatment, either following their regular diet (controls) or with three adjunctive 5-day courses of FMD (test). Blood and gingival crevicular fluid (GCF) samples were collected to study the levels of inflammatory biomarkers, along with clinical parameters and patient-reported outcome measurements (PROMs). All patients were followed up at days 1, 7, 45, 90 and 180 post treatment, and food diaries were completed. Exploratory repeated measures ANOVA and non-parametric tests were used.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Twenty-seven patients completed the 6-month follow-up. Feasibility criteria were satisfied. Baseline serum high-sensitivity (hs)-CRP increased on Day 1 in both groups and then decreased. Exploratory longitudinal analyses indicated temporal changes in hs-CRP and selected GCF biomarkers (including MMP-8, CRP, IL-6, IL-1<i>α</i> and IL-1<i>β</i>), with descriptive trends towards lower values at later time points in the FMD group observed for hs-CRP, MMP-8 and IL-6. No inter-group periodontal clinical differences were detected. Only minimal adverse events were reported in the FMD group, with no inter-group differences in PROMs and clinical outcomes.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Three cycles of FMD, adjunctive to step 2, were associated with a reduction of the inflammatory response after subgingival instrumentation, although these changes did not benefit clinical parameters. Given the exploratory nature of the analyses and the multiple comparisons performed, these findings should be interpreted with caution.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 8","pages":"1254-1264"},"PeriodicalIF":8.3,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70139","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148218028","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to ‘‘Clinical Efficacy of Interventions Based on Professional Mechanical Plaque Removal in the Treatment of Dental Biofilm–Induced Gingivitis: A Systematic Review and Meta‐Analysis’’","authors":"","doi":"10.1111/jcpe.70149","DOIUrl":"https://doi.org/10.1111/jcpe.70149","url":null,"abstract":"","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"212 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148355685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Non-Plaque-Induced Gingival Conditions: A Systematic Review-Part 2: Inflammatory and Immune Conditions; Neoplasms; and Gingival Pigmentation.","authors":"Maria Clotilde Carra, Cecile Wasielewski, Bruno Špiljak, Maja Sabalić-Schoener, Sebastien Jungo, Ivan Alajbeg","doi":"10.1111/jcpe.70152","DOIUrl":"https://doi.org/10.1111/jcpe.70152","url":null,"abstract":"<p><strong>Aims: </strong>This systematic review assesses current evidence on the management of non-plaque (dental biofilm)-induced gingival diseases and conditions (NPIGDs), including (i) inflammatory and immune conditions, (ii) neoplasms and (iii) gingival pigmentations.</p><p><strong>Material and methods: </strong>To address the question 'How should NPIGDs affecting the gingiva be managed?' specific searches were conducted in four electronic databases to identify clinical studies evaluating the effectiveness of any intervention for NPIGDs in patients of all ages. All descriptive and analytical study designs were eligible, provided they described conditions presenting at the gingival level.</p><p><strong>Results: </strong>Over a total of 6111 articles initially identified, 461 met inclusion criteria. Reports ranged from case reports to randomised controlled trials. Overall evidence quality was moderate to very low, reflecting highly heterogeneous literature published over the past 50 years. Treatment approaches varied substantially depending on whether the condition was primarily gingival (e.g., leukoplakia, pigmentations) or a gingival manifestation of a systemic disease (e.g., leukaemic infiltration). Potentially malignant and malignant gingival lesions required careful evaluation and biopsy for diagnosis, followed by surgical management, typically involving radical excision. Gingival pigmentations were most often managed by dentists and, generally, only when patients expressed aesthetic concerns.</p><p><strong>Conclusions: </strong>NPIGD management is challenging due to the heterogeneity of these conditions and often requires multidisciplinary care. Dental professionals must conduct thorough clinical assessments, identify prognostic factors, perform appropriate screening and diagnosis and select the most suitable treatment strategy-which frequently includes referral to medical or oral medicine specialists, especially for potentially malignant or malignant gingival lesions.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":8.3,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148283389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatiotemporally Responsive BEC Nanoplatform Targets Neutrophils to Reprogram Immune Crosstalk and Alleviate Periodontal Inflamm-Ageing in Periodontitis","authors":"Jiaxin Luo,&nbsp;Jingxia Chen,&nbsp;Sicong Ren,&nbsp;Mucong Li,&nbsp;Shaobo Zhai,&nbsp;Sheng Chen,&nbsp;Jian Feng,&nbsp;Zhibo Wang,&nbsp;Hanchi Wang,&nbsp;Yanmin Zhou","doi":"10.1111/jcpe.70133","DOIUrl":"10.1111/jcpe.70133","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To investigate the underlying mechanism of immune cell crosstalk in periodontal inflammatory ageing and to explore potential pharmaceutical interventions for safely reversing this process.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Bovine serum albumin–epigallocatechin gallate–copper nanoparticles (BEC NPs) were synthesised by coating a bovine serum albumin membrane onto an epigallocatechin gallate–copper–phenolic network. BEC NPs' regulatory impacts on the fate of neutrophils and macrophages were evaluated through immunofluorescence staining, PCR, RNA sequencing and Western blot analysis. Their anti-senescence effects on gingival fibroblasts were assessed using cell migration assays and SA-β-gal staining, while an experimental periodontitis rat model was established to validate the in vitro findings.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Accumulating evidence indicated that pH-responsive nanoparticles alleviated periodontal inflamm-ageing through modulation of neutrophil–macrophage crosstalk. Functional analyses revealed that BEC NPs suppressed neutrophil extracellular trap formation via dual mechanisms: reactive oxygen species scavenging to sustain mitochondrial integrity, and actin cytoskeleton stabilisation to inhibit nuclear translocation of neutrophil elastase and myeloperoxidase. In vivo assessment demonstrated that BEC NPs exhibited a favourable biosafety profile and significant therapeutic efficacy in suppressing the progression of periodontal inflammation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This study presents a smart nanosystem-based ‘endogenous homeostasis reconstruction’ strategy, offering programmable, early-stage intervention for periodontal inflamm-ageing with considerable clinical translation prospects.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 7","pages":"1031-1045"},"PeriodicalIF":6.8,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Proteome to Therapeutics: A Multi-Database Approach to Drug Discovery in Periodontitis—An Exploratory Pilot Study","authors":"Taisir Bozo,&nbsp;Annette Murr,&nbsp;Birte Holtfreter,&nbsp;Sebastian-Edgar Baumeister,&nbsp;Peter Meisel,&nbsp;Uwe Völker,&nbsp;Werner Weitschies,&nbsp;Henry Völzke,&nbsp;Philipp Kanzow,&nbsp;Elke Hammer,&nbsp;Manuela Gesell Salazar,&nbsp;Thomas Kocher","doi":"10.1111/jcpe.70129","DOIUrl":"10.1111/jcpe.70129","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Introduction</h3>\u0000 \u0000 <p>This explanatory pilot study presents a workflow to identify approved drugs, which could be repurposed for periodontitis therapy using salivary proteomics combined with drug-target database screening.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Proteomic analyses of saliva using LC–MS/MS were conducted in two independent settings: a cohort (sub-study I, <i>N</i> = 187) and case–control (sub-study II, <i>N</i> = 72). Statistical analyses were performed using both stringent and lenient criteria. Under the stringent (lenient) criteria, proteins were considered potential targets if they showed a <i>q</i>-value &lt; 0.05 (&lt; 0.1) in the cohort study and a <i>p</i>-value &lt; 0.05 together with an absolute fold change &gt; 1.5 (1.3) in the case–control study. Four databases were searched to assess whether these proteins are known targets of approved drugs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In the stringent (lenient) analysis one (26) proteins were associated with periodontitis in both studies, of which one (nine) were reported drug targets and one (seven) of those were potential drug targets in periodontitis. Target screening in databases identified 3/11 approved drugs, with eight of them having potential for repurposing for periodontal treatment. Several of these drugs (e.g., HDAC inhibitors, sivelestat, compstatin derivatives, auranofin, artenimol and bortezomib) have already shown efficacy in preclinical models of periodontal host modulation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>This exploratory pilot study provided insights into potential therapeutic targets and potential drugs for periodontitis treatment. This workflow may help to identify approved drugs that could be repurposed for periodontal treatment.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 7","pages":"990-1004"},"PeriodicalIF":6.8,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70129","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics Augment the Effect of Non-Surgical Periodontal Treatment—A Randomised, Double-Blinded, Placebo-Controlled Trial","authors":"Christine Marie Lundtorp-Olsen,&nbsp;Sara Vallentin Raae Andersen,&nbsp;Laura Massarenti,&nbsp;Mervi Gürsoy,&nbsp;Annina van Splunter,&nbsp;Floris J. Bikker,&nbsp;Ulvi Kahraman Gursoy,&nbsp;Merete Markvart,&nbsp;Christian Damgaard,&nbsp;Daniel Belstrøm","doi":"10.1111/jcpe.70136","DOIUrl":"10.1111/jcpe.70136","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim</h3>\u0000 \u0000 <p>To determine the effect of probiotic lozenges containing <i>Lacticaseibacillus rhamnosus PB01</i>, <i>Latilactobacillus curvatus EB10</i> and xylitol after non-surgical periodontal treatment (NSPT) on changes in microbial composition. The secondary aims were to assess the clinical and immunological impact of probiotic consumption.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Eighty adults with stage II or III periodontitis were enrolled and received NSPT at baseline, followed by a 12-week consumption of probiotics or placebo. Microbial sampling and clinical examination were performed at baseline, Week 6 and Week 12. The subgingival microbiota was analysed using 16S sequencing, the salivary microbiota by metagenomic sequencing and selected cytokines and proteases in saliva by bead-based immunoassay.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Sixty-one participants completed the trial (probiotics <i>n</i> = 32, placebo <i>n</i> = 29). At Week 12, \u0000 <i>Treponema socranskii</i>\u0000 , \u0000 <i>Selenomonas sputigena</i>\u0000 , \u0000 <i>Dialister pneumosintes</i>\u0000 , \u0000 <i>Dialister invisus</i>\u0000 , \u0000 <i>Anaeroglobus geminatus</i>\u0000 and \u0000 <i>Fusobacterium nucleatum</i>\u0000 were significantly associated with the placebo group, while \u0000 <i>Streptococcus sanguinis</i>\u0000 , \u0000 <i>Neisseria elongata</i>\u0000 and <i>Neisseria oralis</i> were associated with the probiotic group. Bleeding on probing percentage (BoP%) and number of periodontal pockets (PPD) ≥ 5 mm decreased significantly more in the probiotic group compared to the placebo group (<i>p</i> &lt; 0.05).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>The tested probiotic supplement resulted in an additional short-term decrease in periodontitis-associated species along with greater improvements in BoP% and PPD ≥ 5 mm 12 weeks post-NSPT, compared to the placebo group.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 7","pages":"1016-1030"},"PeriodicalIF":6.8,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70136","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingival and Periodontal Diseases and Conditions in Children and Adolescents: Consensus Report","authors":"Iain Chapple,&nbsp;Sotiria Gizani,&nbsp;Mariano Sanz,&nbsp;Dominique Declerck,&nbsp;Phoebus Madianos,&nbsp;Valerie Clerehugh,&nbsp;Monique Danser,&nbsp;Esti Davidovich,&nbsp;Janet Davies,&nbsp;Nikos Donos,&nbsp;Elisabeth Dursun,&nbsp;Elena Figuero,&nbsp;Filippo Graziani,&nbsp;Mervi Gursoy,&nbsp;Soren Jepsen,&nbsp;Clara Joseph,&nbsp;Moritz Kebschull,&nbsp;Norbert Kraemer,&nbsp;Tina Lambrinaki,&nbsp;Rodrigo López,&nbsp;David J. Manton,&nbsp;Joerg Meyle,&nbsp;Ana Molina,&nbsp;Lior Shapira,&nbsp;Cheryl Somani,&nbsp;Georgios Tsilingaridis,&nbsp;Svante Twetman,&nbsp;Nicola West,&nbsp;Biniyam Wondimu,&nbsp;Ferranti Wong,&nbsp;David Herrera","doi":"10.1111/jcpe.70033","DOIUrl":"10.1111/jcpe.70033","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>The objectives of this Focused Workshop were to update the epidemiology, aetiology, risk factors, diagnosis and management of gingival and periodontal diseases and conditions in children and adolescents, and to explore the applicability of the 2018 Classification in children and adolescents.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The Workshop discussions were informed by three specifically commissioned systematic reviews covering gingival and periodontal diseases and conditions, in systemically healthy children and adolescents, or in children and adolescents with systemic conditions.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Over 70 genetic, congenital and acquired systemic conditions that impact the periodontal tissues were identified, with levels of evidence graded as very low, low or moderate. Gingival diseases and conditions in systemically healthy children and adolescents were identified, alongside local predisposing and systemic modifying factors. Periodontitis and other periodontal conditions in the 2018 Classification System also apply to children and adolescents; however, there are challenges with periodontal probing in the primary and mixed dentition.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Periodontal tissues in children and adolescents differ from those in adults and require special consideration, accounting for their stage of development and predisposing and modifying factors unique to younger patients, which may confound accurate diagnosis, prognostication and management. Specific approaches to screening, examination and treatment are necessary for safe and effective management in this patient group.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 7","pages":"1068-1099"},"PeriodicalIF":6.8,"publicationDate":"2026-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147680375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}