Journal of Clinical Periodontology最新文献

{"title":"Spatiotemporally Responsive BEC Nanoplatform Targets Neutrophils to Reprogram Immune Crosstalk and Alleviate Periodontal Inflamm-Ageing in Periodontitis.","authors":"Jiaxin Luo, Jingxia Chen, Sicong Ren, Mucong Li, Shaobo Zhai, Sheng Chen, Jian Feng, Zhibo Wang, Hanchi Wang, Yanmin Zhou","doi":"10.1111/jcpe.70133","DOIUrl":"https://doi.org/10.1111/jcpe.70133","url":null,"abstract":"<p><strong>Aim: </strong>To investigate the underlying mechanism of immune cell crosstalk in periodontal inflammatory ageing and to explore potential pharmaceutical interventions for safely reversing this process.</p><p><strong>Materials and methods: </strong>Bovine serum albumin-epigallocatechin gallate-copper nanoparticles (BEC NPs) were synthesised by coating a bovine serum albumin membrane onto an epigallocatechin gallate-copper-phenolic network. BEC NPs' regulatory impacts on the fate of neutrophils and macrophages were evaluated through immunofluorescence staining, PCR, RNA sequencing and Western blot analysis. Their anti-senescence effects on gingival fibroblasts were assessed using cell migration assays and SA-β-gal staining, while an experimental periodontitis rat model was established to validate the in vitro findings.</p><p><strong>Results: </strong>Accumulating evidence indicated that pH-responsive nanoparticles alleviated periodontal inflamm-ageing through modulation of neutrophil-macrophage crosstalk. Functional analyses revealed that BEC NPs suppressed neutrophil extracellular trap formation via dual mechanisms: reactive oxygen species scavenging to sustain mitochondrial integrity, and actin cytoskeleton stabilisation to inhibit nuclear translocation of neutrophil elastase and myeloperoxidase. In vivo assessment demonstrated that BEC NPs exhibited a favourable biosafety profile and significant therapeutic efficacy in suppressing the progression of periodontal inflammation.</p><p><strong>Conclusion: </strong>This study presents a smart nanosystem-based 'endogenous homeostasis reconstruction' strategy, offering programmable, early-stage intervention for periodontal inflamm-ageing with considerable clinical translation prospects.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From the Proteome to Therapeutics: A Multi-Database Approach to Drug Discovery in Periodontitis-An Exploratory Pilot Study.","authors":"Taisir Bozo, Annette Murr, Birte Holtfreter, Sebastian-Edgar Baumeister, Peter Meisel, Uwe Völker, Werner Weitschies, Henry Völzke, Philipp Kanzow, Elke Hammer, Manuela Gesell Salazar, Thomas Kocher","doi":"10.1111/jcpe.70129","DOIUrl":"https://doi.org/10.1111/jcpe.70129","url":null,"abstract":"<p><strong>Introduction: </strong>This explanatory pilot study presents a workflow to identify approved drugs, which could be repurposed for periodontitis therapy using salivary proteomics combined with drug-target database screening.</p><p><strong>Methods: </strong>Proteomic analyses of saliva using LC-MS/MS were conducted in two independent settings: a cohort (sub-study I, N = 187) and case-control (sub-study II, N = 72). Statistical analyses were performed using both stringent and lenient criteria. Under the stringent (lenient) criteria, proteins were considered potential targets if they showed a q-value < 0.05 (< 0.1) in the cohort study and a p-value < 0.05 together with an absolute fold change > 1.5 (1.3) in the case-control study. Four databases were searched to assess whether these proteins are known targets of approved drugs.</p><p><strong>Results: </strong>In the stringent (lenient) analysis one (26) proteins were associated with periodontitis in both studies, of which one (nine) were reported drug targets and one (seven) of those were potential drug targets in periodontitis. Target screening in databases identified 3/11 approved drugs, with eight of them having potential for repurposing for periodontal treatment. Several of these drugs (e.g., HDAC inhibitors, sivelestat, compstatin derivatives, auranofin, artenimol and bortezomib) have already shown efficacy in preclinical models of periodontal host modulation.</p><p><strong>Conclusion: </strong>This exploratory pilot study provided insights into potential therapeutic targets and potential drugs for periodontitis treatment. This workflow may help to identify approved drugs that could be repurposed for periodontal treatment.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics Augment the Effect of Non-Surgical Periodontal Treatment-A Randomised, Double-Blinded, Placebo-Controlled Trial.","authors":"Christine Marie Lundtorp-Olsen, Sara Vallentin Raae Andersen, Laura Massarenti, Mervi Gürsoy, Annina van Splunter, Floris J Bikker, Ulvi Kahraman Gursoy, Merete Markvart, Christian Damgaard, Daniel Belstrøm","doi":"10.1111/jcpe.70136","DOIUrl":"https://doi.org/10.1111/jcpe.70136","url":null,"abstract":"<p><strong>Aim: </strong>To determine the effect of probiotic lozenges containing Lacticaseibacillus rhamnosus PB01, Latilactobacillus curvatus EB10 and xylitol after non-surgical periodontal treatment (NSPT) on changes in microbial composition. The secondary aims were to assess the clinical and immunological impact of probiotic consumption.</p><p><strong>Materials and methods: </strong>Eighty adults with stage II or III periodontitis were enrolled and received NSPT at baseline, followed by a 12-week consumption of probiotics or placebo. Microbial sampling and clinical examination were performed at baseline, Week 6 and Week 12. The subgingival microbiota was analysed using 16S sequencing, the salivary microbiota by metagenomic sequencing and selected cytokines and proteases in saliva by bead-based immunoassay.</p><p><strong>Results: </strong>Sixty-one participants completed the trial (probiotics n = 32, placebo n = 29). At Week 12, Treponema socranskii, Selenomonas sputigena, Dialister pneumosintes, Dialister invisus, Anaeroglobus geminatus and Fusobacterium nucleatum were significantly associated with the placebo group, while Streptococcus sanguinis, Neisseria elongata and Neisseria oralis were associated with the probiotic group. Bleeding on probing percentage (BoP%) and number of periodontal pockets (PPD) ≥ 5 mm decreased significantly more in the probiotic group compared to the placebo group (p < 0.05).</p><p><strong>Conclusion: </strong>The tested probiotic supplement resulted in an additional short-term decrease in periodontitis-associated species along with greater improvements in BoP% and PPD ≥ 5 mm 12 weeks post-NSPT, compared to the placebo group.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the Potential of the PerioAI System to Support Periodontal Clinical Decision Making: A Proof-of-Principle Study.","authors":"Hairui Li, Yuan Li, Minhui Tan, Zhiming Cui, Dinggang Shen, Andrea Roccuzzo, Maurizio S Tonetti","doi":"10.1111/jcpe.70138","DOIUrl":"https://doi.org/10.1111/jcpe.70138","url":null,"abstract":"<p><strong>Aim: </strong>To explore the potential of PerioAI, an artificial intelligence system integrating intraoral scanning and cone-beam CT, to automatically measure gingival margin-to-bone distance (GBD) and convert it into AI-derived probing depth (AI-PD), and to evaluate whether AI-PD may provide additional information to support periodontal clinical decision making when radiographic imaging represents the primary source of available periodontal information.</p><p><strong>Materials and methods: </strong>This cross-sectional proof-of-principle study included 53 patients with periodontitis (1298 teeth, 7788 sites). GBD measurements were converted to AI-PD using validated formulas. Clinical decision making (prognosis and treatment planning) was evaluated by one periodontist under three information conditions: (i) orthopantomogram (OPG) + original periodontal chart (used to establish the reference clinical decision); (ii) OPG-only; and (iii) OPG + AI-PD. Using the reference clinical decision, agreement rates for clinical decisions obtained under the two information conditions (OPG-only and OPG + AI-PD) were calculated and compared, and the risk of overtreatment was also assessed.</p><p><strong>Results: </strong>Compared with OPG-only, the OPG + AI-PD condition showed higher agreement rate with the reference clinical decision, indicating that PerioAI may provide additional information for clinical decision making. Patient-level average agreement rates increased from 77.6% to 84.7% for prognosis (p < 0.05) and from 78.2% to 84.3% for treatment planning (p < 0.05). Tooth-level agreement rates improved from 78.1% to 86.0% for prognosis (p < 0.05) and from 78.8% to 85.4% for treatment planning (p < 0.05). The addition of AI-PD was associated with a 42.3% reduction in overtreatment risk (Steps 1-2 vs. Step 3) and a 98.5% reduction in the risk of tooth extraction (Step 3 vs. extraction).</p><p><strong>Conclusions: </strong>When combined with radiographic information, PerioAI shows potential to provide incremental information for clinical decision making. Future research should integrate additional periodontal parameters and validate the approach in larger and more diverse populations.</p>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":" ","pages":""},"PeriodicalIF":6.8,"publicationDate":"2026-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147815744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Periodontitis and Methodological Aspects of the 2018 EFP/AAP Classification-The Study of Health in Pomerania (SHIP-TREND).","authors":"Sonya Nafz,Thomas Kocher,Christiane Pink,Henry Völzke,Philipp Kanzow,Birte Holtfreter","doi":"10.1111/jcpe.70137","DOIUrl":"https://doi.org/10.1111/jcpe.70137","url":null,"abstract":"AIMTo estimate the prevalence of periodontitis and highlight methodological issues using the Application of the 2018 Periodontal Status Classification to Epidemiological Survey Data (ACES) framework.MATERIALS AND METHODSWe used data from the population-based Study of Health in Pomerania (SHIP-TREND; age 20-84 years). Periodontal status was recorded using a half-mouth protocol. We estimated the prevalence of periodontitis, evaluated changes in the assignment of stages to combinations of available complexity factors, associated periodontal status with 7-year tooth loss and illustrated agreement with the Centers for Disease Control and Prevention/American Academy of Periodontology (CDC/AAP) classification.RESULTSThe total prevalence of periodontitis was 78.6%; 18.0% and 17.4% were Stage III and Stage IV, respectively. Grade C was exhibited by 49.1% of Stage III cases and 37.8% of Stage IV cases. Assignment of stage changed only marginally when complexity factors were considered in addition to interdental clinical attachment levels. Three percent of Stage IV cases and 15.2% of non-classified cases became toothless. Adjusted incidence rates for tooth loss were 3.60 for Stage III cases, 6.55 for non-classified cases and 8.69 for Stage IV cases. Severe cases (CDC/AAP) corresponded primarily to Stage III (42.9%) and Stage IV (53.0%) cases.CONCLUSIONSFor public health purposes, caution should be exercised when translating the periodontitis prevalence according to the ACES framework into treatment requirements. Furthermore, complex and effective interventions are required for non-classified and Stage IV cases in order to prevent tooth loss and edentulism.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"1 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147754911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management of Periodontal Abscesses and Endodontic-Periodontal Lesions-A Systematic Review.","authors":"Henrik Dommisch,Daniela Hoedke,Marina Dourou,Elena Calciolari,David Herrera,Selena Toma","doi":"10.1111/jcpe.70135","DOIUrl":"https://doi.org/10.1111/jcpe.70135","url":null,"abstract":"AIMTo evaluate the management of periodontal abscesses and endodontic-periodontal lesions (EPLs).METHODSSeven focused questions addressed outcomes such as pocket depth (PD), bleeding on probing (BOP), tooth loss or ovoid elevation. The literature search covered three electronic databases and manual sources for intervention studies. Meta-analyses were not feasible.RESULTSFor periodontal abscesses and EPLs, a total of 987 and 545 articles were identified, and 17 and 44 were selected for full-text analysis, respectively. For periodontal abscesses, three randomised clinical trials (RCTs) and two prospective case series were included, and it was shown that combined approaches led to reductions in PD of 2-3 mm, BOP from 100% to 30%-44% and ovoid elevation from 92%-100% to 0%-11%. Abscess recurrence was found in 13.3%-23% within 15 months follow-up. For EPLs, nine RCTs, six prospective and two retrospective studies and one case series were included, and the overall synthesis showed that PD reductions ranged from 2.5 to 9.3 mm and tooth loss rate from 0% to 25% (3-120 months follow-up). Low to high risk of bias was evident.CONCLUSIONSPeriodontal abscesses can effectively be managed by initial drainage, followed by periodontal therapy, based on limited and weak evidence. For EPLs, endodontic and periodontal (non-surgical and surgical) interventions have been investigated, alone or in combination, but the heterogeneity of study designs and small sample sizes preclude strong conclusions.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"32 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147731417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Orthodontic Tooth Movement on Intrabony Periodontal Defects After Guided Tissue Regeneration—Part I: Human Histologic Evaluation of 20 Consecutive Cases","authors":"Pal Nagy, Peter Windisch, Balint Nemes, Peter Schupbach, Andrea Dobos, Andreas Stavropoulos","doi":"10.1111/jcpe.70134","DOIUrl":"https://doi.org/10.1111/jcpe.70134","url":null,"abstract":"Aim To histologically evaluate the healing of intrabony periodontal defects treated with guided tissue regeneration (GTR), if it is combined with orthodontic tooth movement (OTM) or used as a sole treatment. Materials and Methods Twenty subjects requiring regenerative periodontal therapy and OTM were treated with the use of extended, coronally advanced flaps according to the GTR techniques with the utilization of deproteinized bovine bone mineral (DBBM) particles. Patients either received early initiation of OTM (test) or had their teeth splinted (control) after the surgical intervention. Re‐entry procedures were scheduled 9 months postoperatively to obtain a biopsy from the previous defect sites. The primary outcome variable comprised histological and histomorphometric analysis. Results Control group cases ( <jats:italic>n</jats:italic> = 9) revealed nice embedding of graft particles into newly formed bone, which were predominantly present in the central and apical third of the biopsy samples. The coronally located DBBM was more often encapsulated in the connective tissue. Test samples ( <jats:italic>n</jats:italic> = 10), both at the tension and pressure sites, demonstrated incorporation of a reduced graft ratio into newly formed bone. Ongoing bone formation and the presumably orthodontic‐induced remodeling also interfered with the bone substitute material. Histomorphometry showed a distribution of 17.4% versus 33.9% new bone ( <jats:italic>p</jats:italic> = 0.011), 33.2% versus 16.3% graft ratio ( <jats:italic>p</jats:italic> = 0.001) and 49.4% versus 49.7% soft tissue components ( <jats:italic>p</jats:italic> = 0.74) in the control versus test groups, respectively. Conclusions Early initiation of tooth movement does not appear to adversely affect periodontal bone healing. A pronounced graft reduction and new bone formation in test patients, compared to those in controls, occurred presumably due to the effects of orthodontic‐induced bone remodeling.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"19 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147725922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Periodontal Diseases and Conditions in Children and Adolescents Associated With Systemic Disorders: A Systematic Review.","authors":"Ana Molina,Joerg Meyle,Diana Ram,Yong-Hee Patricia Chun,María Eugenia Colmenares,Esti Davidovich","doi":"10.1111/jcpe.70109","DOIUrl":"https://doi.org/10.1111/jcpe.70109","url":null,"abstract":"AIMTo systematically evaluate congenital and acquired systemic diseases and conditions in children and adolescents, and their impact on periodontal tissues as well as influence on the onset and progression of gingivitis and periodontitis.MATERIAL AND METHODSA systematic search was performed in three electronic databases to identify studies reporting on the epidemiology, aetiology, risk factors, diagnosis, prevention and treatment of systemic diseases and conditions in children and adolescents < 18 years, affecting periodontal tissues and modifying the course/outcomes of periodontal diseases.RESULTSA total of 463 studies were included in the qualitative analysis, reporting on 71 different diseases and conditions. The medical conditions identified were classified into two groups, based upon the nature of pathogenic mechanisms affecting the periodontium: (1) genetic, congenital and acquired systemic conditions that impact periodontal tissue structure, and (2) systemic diseases and conditions that impact the onset/progression of periodontal diseases.CONCLUSIONA vast group of systemic congenital and acquired diseases and conditions affecting children and adolescents was identified that may impact the morphology, function and/or pathophysiological processes of the periodontal tissues and affect the onset/progression of periodontal diseases, which in some cases leads to severe forms of gingival inflammation, premature loss of attachment and tooth loss.","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"66 1","pages":""},"PeriodicalIF":6.7,"publicationDate":"2026-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147702217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Edentulism With Subsequent Biological Age: A Cohort Study","authors":"Yusuke Matsuyama,&nbsp;Sakura Kiuchi,&nbsp;Jun Aida","doi":"10.1111/jcpe.70106","DOIUrl":"10.1111/jcpe.70106","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Biological ageing is characterised by a gradual decline in physiological functions, resulting in increased susceptibility to diseases and mortality. We investigated the relationship between edentulism and biological ageing among adults in England.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Data were drawn from participants in the English Longitudinal Study of Ageing who completed three consecutive biennial waves (waves 2–3–4 or 4–5–6), yielding 1889 individuals providing 2390 three-wave panel observations (mean age at baseline 68.1; men 47.7%). Edentulism was assessed in waves 3 and 5. Biological ageing was estimated using the Klemera and Doubal method, integrating multiple biomarkers assessed in waves 2, 4 and 6. Linear regression with clustered standard errors was employed to evaluate the association between edentulism and biological age in the subsequent wave (i.e., 2 years after the assessment of edentulism). Models were adjusted for pre-exposure confounders, including biological age, gender, socioeconomic status, existing health conditions and functional limitation assessed in the prior wave (i.e., 2 years before the assessment of edentulism).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Participants with edentulism exhibited a 5.8-year greater biological age than those with some remaining teeth in the subsequent waves. After adjusting for confounding variables, edentulism was associated with a 0.82-year greater biological age than those with some remaining teeth (95% confidence interval: 0.40–1.24) in the subsequent waves.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Edentulism was longitudinally associated with a greater biological age. Strategies that incorporate oral health promotion, particularly the prevention of tooth loss, may contribute to healthy ageing.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 5","pages":"752-759"},"PeriodicalIF":6.8,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70106","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Untargeted Metabolomic and Lipidomic Profiles of Gingival Crevicular Fluid in the Context of Periodontitis","authors":"Caroline H. Henderzahs,&nbsp;Chiaki Yamada,&nbsp;Alexandr Morozov,&nbsp;Thelmalane Yalartai,&nbsp;Vanchit John,&nbsp;Hawra AlQallaf,&nbsp;Alexandru Movila","doi":"10.1111/jcpe.70105","DOIUrl":"10.1111/jcpe.70105","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Aim(s)</h3>\u0000 \u0000 <p>This cross-sectional clinical study aimed to explore the untargeted metabolomic and lipidomic profiles in gingival crevicular fluid (GCF) obtained from healthy individuals and patients with periodontitis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>GCF was collected from 17 periodontally healthy and 19 periodontitis patients. For the unbiased characterisation of positively and negatively charged polar metabolite and lipid compounds, we employed hydrophilic interaction and reversed-phase liquid chromatography/mass spectrometry platforms, followed by a semisupervised deep learning-based approach for metabolomic peak curation and data analysis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>A total of 256 metabolites were identified with Metabolomics Standards Initiative (MSI) confidence Levels 1–3, including 198 elevated and 58 diminished compounds in periodontal lesions (<i>q</i> &lt; 0.05, |log₂-transformed Fold Change (FC)| &gt; 1). Periodontitis samples exhibited a significant positive accumulation of purine degradation and ceramide metabolites, along with a negative regulation of oxy fatty acids metabolism. Additionally, we observed a significant increase in established periodontitis biomarkers, including N-acetylneuraminic acid, citrulline and 2-pyrrolidineacetic acid. The study also characterised distinct differences in bacterial and fungal metabolite profiles between the healthy and diseased samples.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These findings suggest that untargeted metabolomic screening of GCF may significantly improve our understanding of biochemical changes between healthy tissue and periodontitis. This knowledge is pivotal for the development of a precision paradigm in periodontitis.</p>\u0000 </section>\u0000 </div>","PeriodicalId":15380,"journal":{"name":"Journal of Clinical Periodontology","volume":"53 5","pages":"774-783"},"PeriodicalIF":6.8,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/jcpe.70105","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146149915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}