Journal of biochemistry最新文献_第4页

Two-sided function of osteopontin during osteoblast differentiation. 成骨细胞分化过程中骨桥蛋白的双面功能。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-02-05 DOI: 10.1093/jb/mvae080

Fredy Mardiyantoro, Norika Chiba, Chang-Hwan Seong, Ryohei Tada, Tomokazu Ohnishi, Norifumi Nakamura, Tetsuya Matsuguchi

{"title":"Two-sided function of osteopontin during osteoblast differentiation.","authors":"Fredy Mardiyantoro, Norika Chiba, Chang-Hwan Seong, Ryohei Tada, Tomokazu Ohnishi, Norifumi Nakamura, Tetsuya Matsuguchi","doi":"10.1093/jb/mvae080","DOIUrl":"10.1093/jb/mvae080","url":null,"abstract":"Osteopontin (OPN) is expressed in various cell types including osteoblasts. OPN expression level is robustly increased during osteoblast differentiation. Although OPN was initially found as a secretory protein (sOPN), recent reports identified the intracellular isoform of OPN (iOPN). Distinct functions of each OPN isoform in osteoblasts, however, are not well established. Here, using the Tet-On inducible expression system, we examined the role of each OPN isoform during osteoblast differentiation. Induced overexpression of wild type OPN (wtOPN), which includes both sOPN and iOPN, significantly increased matrix mineralization and osteogenic marker gene expression during osteogenic differentiation induced by either ascorbic acid or bone morphogenetic protein (BMP) 9. In contrast, these osteogenic differentiation processes were significantly inhibited by the specific overexpression of iOPN. Furthermore, the addition of recombinant OPN or neutralizing anti-OPN antibody to the culture medium exerted promotive or inhibitory effect on osteoblast differentiation, respectively. These data strongly indicate that iOPN exerts inhibitory effects on osteoblast differentiation, whereas sOPN exerts positive effects. We also found that the secretion process of OPN is positively regulated by c-Jun N-terminal kinase (JNK) activity in osteoblasts.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"121-131"},"PeriodicalIF":2.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ionic control of small GTPase HRas using calmodulin. 钙调素对小GTPase HRas的离子控制。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-02-05 DOI: 10.1093/jb/mvae089

Yassine Sabek, Ziyun Zhang, Nobuyuki Nishibe, Shinsaku Maruta

引用次数: 0

GPLD1+ cancer stem cells contribute to chemotherapy resistance and tumour relapse in intestinal cancer. GPLD1+肿瘤干细胞参与肠癌化疗耐药和肿瘤复发。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-02-05 DOI: 10.1093/jb/mvae082

Taisuke Mizoo, Takeru Oka, Osamu Sugahara, Takafumi Minato, Tsunaki Higa, Keiichi I Nakayama

{"title":"GPLD1+ cancer stem cells contribute to chemotherapy resistance and tumour relapse in intestinal cancer.","authors":"Taisuke Mizoo, Takeru Oka, Osamu Sugahara, Takafumi Minato, Tsunaki Higa, Keiichi I Nakayama","doi":"10.1093/jb/mvae082","DOIUrl":"10.1093/jb/mvae082","url":null,"abstract":"Cancer stem cells (CSCs) play a central role in cancer progression, therapy resistance, and disease recurrence. With the use of a quadruple-mutant mouse intestinal cancer organoid model and single-cell RNA-sequencing analysis, we have now identified glycosylphosphatidylinositol-specific phospholipase D1 (GPLD1), an enzyme that catalyzes the cleavage of glycosylphosphatidylinositol (GPI) anchors of membrane proteins, as a marker of slowly cycling CSCs. Ablation of Gpld1+ cells in combination with 5-fluorouracil treatment greatly attenuated cell viability in and regrowth of the intestinal cancer organoids. In addition, we identified serine protease 8 (PRSS8) as a key substrate of GPLD1 in human colorectal cancer cells. GPLD1 cleaves the GPI anchor of PRSS8 and thereby mediates release of the protease from the plasma membrane, resulting in the activation of Wnt signalling and promotion of the epithelial-mesenchymal transition (EMT) in the cancer cells. Pharmacological inhibition of GPLD1 suppressed Wnt signalling activity and EMT in association with upregulation of the amount of functional PRSS8 at the plasma membrane. Our findings suggest that targeting of GPLD1 in colorectal cancer might contribute to a new therapeutic strategy that is based on suppression of Wnt signalling and EMT-related cancer progression driven by CSCs.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"105-119"},"PeriodicalIF":2.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142914950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RMST: a long noncoding RNA involved in cancer and disease. RMST：一种与癌症和疾病有关的长链非编码RNA。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-02-05 DOI: 10.1093/jb/mvae083

Hidenori Tani

{"title":"RMST: a long noncoding RNA involved in cancer and disease.","authors":"Hidenori Tani","doi":"10.1093/jb/mvae083","DOIUrl":"10.1093/jb/mvae083","url":null,"abstract":"Long non-coding RNA rhabdomyosarcoma 2-associated transcript (RMST) is a crucial regulator in various biological processes, particularly in neurogenesis and cancer progression. This review summarizes current knowledge on structure, expression patterns and functional roles across different organs and diseases of RMST. RMST exhibits tissue-specific expression, notably in brain tissues and vascular endothelial cells, and plays a significant role in neuronal differentiation through interaction with SRY-box 2. In cancer, RMST predominantly functions as a tumour suppressor, with context-dependent roles observed across different cancer types. RMST is also implicated in neurological disorders, cardiovascular diseases and Hirschsprung's disease. Mechanistically, RMST acts as a competing endogenous RNA and a transcriptional regulator, interacting with various microRNAs and proteins to modulate gene expression. The potential of RMST as a biomarker and therapeutic target is increasingly recognized, particularly in atherosclerosis and cancer. While current findings are promising, further research is needed to fully elucidate the functions and translate these insights into clinical applications of RMST. This review underscores the significance of RMST in cellular processes and disease pathogenesis, highlighting its potential as a novel target for diagnostic and therapeutic interventions.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"73-78"},"PeriodicalIF":2.1,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142824155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maintenance of the Golgi ribbon structure by the KASH protein Jaw1. KASH 蛋白 Jaw1 维护高尔基体带状结构

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-02-05 DOI: 10.1093/jb/mvae081

Morihisa Fujita

引用次数: 0

A refined method for high-purity isolation of uterine glandular epithelial cells in mouse. 一种高纯度分离小鼠子宫腺上皮细胞的改进方法。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-01-22 DOI: 10.1093/jb/mvaf006

Asmaa M Youssef, Ahmed M Moustafa, Motoharu Hamada, Mayumi Sugiura-Ogasawara, Hisashi Oishi

{"title":"A refined method for high-purity isolation of uterine glandular epithelial cells in mouse.","authors":"Asmaa M Youssef, Ahmed M Moustafa, Motoharu Hamada, Mayumi Sugiura-Ogasawara, Hisashi Oishi","doi":"10.1093/jb/mvaf006","DOIUrl":"https://doi.org/10.1093/jb/mvaf006","url":null,"abstract":"The uterine endometrium consists of luminal epithelium, glandular epithelium, and stromal cells, with uterine glands playing a pivotal role in pregnancy success among mammals. Uterine glands secrete essential factors that regulate embryo development and implantation; however, their cellular biology remains poorly understood. This study presents a refined method for isolating three distinct endometrial cell types with high purity, with a specific emphasis on glandular epithelial cells. The method combines mechanical dissociation, enzymatic digestion, and immunomagnetic separation. The isolated glandular epithelial cells were maintained in culture and exhibited proliferation in response to steroid hormones. Furthermore, estrogen responsiveness was abrogated by Estrogen Receptor 1 (Esr1) knockdown mediated by siRNA. Here, we present an efficient and reproducible method for isolating uterine glandular epithelial cells with high purity, enabling their in vitro maintenance, hormone responsiveness assessment, and functional gene knockdown. These findings establish a robust platform for advancing our understanding of uterine gland biology, facilitating detailed investigations into molecular mechanisms underlying glandular function and their critical roles in establishing pregnancy success. Future research could explore the contribution of these isolated cells to endometrial receptivity and embryo implantation.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High GRWD1 expression may predict clinically aggressive lower grade glioma, skin cutaneous melanoma, and kidney renal clear cell carcinoma carrying wild-type p53: a systematic study based on TCGA data analysis. GRWD1高表达可预测携带野生型p53的临床侵袭性低级别胶质瘤、皮肤黑色素瘤和肾透明细胞癌：一项基于TCGA数据分析的系统研究。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-01-15 DOI: 10.1093/jb/mvaf004

Kota Kayama, Akihiro Ooga, Kouji Hasetsu, Ryoma Kokubo, Nozomi Sugimoto, Masatoshi Fujita

{"title":"High GRWD1 expression may predict clinically aggressive lower grade glioma, skin cutaneous melanoma, and kidney renal clear cell carcinoma carrying wild-type p53: a systematic study based on TCGA data analysis.","authors":"Kota Kayama, Akihiro Ooga, Kouji Hasetsu, Ryoma Kokubo, Nozomi Sugimoto, Masatoshi Fujita","doi":"10.1093/jb/mvaf004","DOIUrl":"https://doi.org/10.1093/jb/mvaf004","url":null,"abstract":"Glutamate-rich WD40 repeat containing 1 (GRWD1) is a novel oncogene/oncoprotein that downregulates the p53 tumor suppressor protein through several mechanisms. One important mechanism involves binding of GRWD1 to RPL11, which competitively inhibits the RPL11-MDM2 interaction and releases RPL11-mediated suppression of MDM2 ubiquitin ligase activity toward p53. Here, we mined the TCGA (The Cancer Genome Atlas) database to gain in-depth insight into the clinical relevance of GRWD1. We found that high expression of GRWD1 is associated with a poor prognosis for lower grade glioma (LGG) of the brain, skin cutaneous melanoma (SKCM), and kidney renal clear cell carcinoma (KIRC) carrying wild-type p53. Further investigations revealed that copy number alterations in the GRWD1 gene are one determinant of GRWD1 expression level. By contrast, even in patients with a diploid GRWD1 gene, high GRWD1 expression was associated with a poor prognosis for LGG, SKCM, and KIRC carrying wild-type p53. Additional studies suggest that some transcriptional factors may be involved in regulation of GRWD1 in cancers with a diploid GRWD1 gene. Taken together, the data presented herein suggest that high expression of GRWD1 may contribute to malignant behavior, and predict a clinically unfavorable prognosis for LGG, SKCM, and KIRC carrying wild-type p53.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142983613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the roles of Lem2 and Bqt4 in lipid metabolism for nuclear envelope maintenance: a novel perspective. 探索 Lem2 和 Bqt4 在维持核包膜的脂质代谢中的作用：一个新的视角

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-01-06 DOI: 10.1093/jb/mvae072

Kei-Ichiro Ishiguro

引用次数: 0

Open and closed structures of L-arginine oxidase by cryo-electron microscopy and X-ray crystallography. 通过冷冻电镜和 X 射线晶体学研究 L-精氨酸氧化酶的开放和封闭结构。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2025-01-06 DOI: 10.1093/jb/mvae070

Hiroki Yamaguchi, Kazutoshi Takahashi, Nobutaka Numoto, Hiroshi Suzuki, Moemi Tatsumi, Akiko Kamegawa, Kouki Nishikawa, Yasuhisa Asano, Toshimi Mizukoshi, Hiroshi Miyano, Yoshinori Fujiyoshi, Masayuki Sugiki

{"title":"Open and closed structures of L-arginine oxidase by cryo-electron microscopy and X-ray crystallography.","authors":"Hiroki Yamaguchi, Kazutoshi Takahashi, Nobutaka Numoto, Hiroshi Suzuki, Moemi Tatsumi, Akiko Kamegawa, Kouki Nishikawa, Yasuhisa Asano, Toshimi Mizukoshi, Hiroshi Miyano, Yoshinori Fujiyoshi, Masayuki Sugiki","doi":"10.1093/jb/mvae070","DOIUrl":"10.1093/jb/mvae070","url":null,"abstract":"L-arginine oxidase (AROD, EC 1.4.3.25) is an oxidoreductase that catalyses the deamination of L-arginine, with flavin adenine dinucleotide (FAD) as a cofactor. Recently identified AROD from Pseudomonas sp. TPU 7192 (PT-AROD) demonstrates high selectivity for L-arginine. This enzyme is useful for accurate assays of L-arginine in biological samples. The structural characteristics of the FAD-dependent AROD, however, remain unknown. Here, we report the structure of PT-AROD at a resolution of 2.3 Å by cryo-electron microscopy. PT-AROD adopts an octameric structure with D4 symmetry, which is consistent with its molecular weight in solution, estimated by mass photometry. Comparative analysis of this structure with that determined using X-ray crystallography reveals open and closed forms of the lid-like loop at the entrance to the substrate pocket. Furthermore, mutation of Glu493, located at the substrate binding site, diminishes substrate selectivity, suggesting that this residue contributes significantly to the high selectivity of PT-AROD.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"27-36"},"PeriodicalIF":2.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11694665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Absolute quantification of BACH1 and BACH2 transcription factors in B and plasma cells reveals their dynamic changes and unique roles. BACH1 和 BACH2 转录因子在 B 细胞和浆细胞中的绝对定量显示了它们的动态变化和独特作用。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae065

Takeshi Kurasawa, Akihiko Muto, Mitsuyo Matsumoto, Kyoko Ochiai, Kazutaka Murayama, Kazuhiko Igarashi

{"title":"Absolute quantification of BACH1 and BACH2 transcription factors in B and plasma cells reveals their dynamic changes and unique roles.","authors":"Takeshi Kurasawa, Akihiko Muto, Mitsuyo Matsumoto, Kyoko Ochiai, Kazutaka Murayama, Kazuhiko Igarashi","doi":"10.1093/jb/mvae065","DOIUrl":"10.1093/jb/mvae065","url":null,"abstract":"Changes in the absolute protein amounts of transcription factors are important for regulating gene expression during cell differentiation and in responses to changes in the cellular and extracellular environment. However, few studies have focused on the absolute quantification of mammalian transcription factors. In this study, we established an absolute quantification method for the transcription factors BACH1 and BACH2, which are expressed in B cells and regulated by direct heme binding. The method used purified recombinant proteins as controls in western blotting and was applied to mouse naïve B cells in the spleen, as well as activated B cells and plasma cells. BACH1 was present in naïve B cells at approximately half the levels of BACH2. In activated B cells, BACH1 decreased compared to naïve B cells, whilst BACH2 increased. In plasma cells, BACH1 increased back to the same extent as in naïve B cells, whilst BACH2 was not detected. Their target genes, Prdm1 and Hmox1, were highly induced in plasma cells. BACH1 was found to undergo degradation with lower concentrations of heme than BACH2. Therefore, BACH1 and BACH2 are similarly abundant in B cells but differ in heme sensitivity, potentially regulating gene expression differently depending on their heme responsiveness.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"449-459"},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0