Journal of biochemistry最新文献_第4页

The 2024 JB Award. 2024年JB奖。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae075

引用次数: 0

Activation of platelet-derived growth factor receptors regulate connective tissue growth factor protein levels via the AKT pathway in malignant mesothelioma cells. 激活血小板衍生生长因子受体可通过 AKT 途径调节恶性间皮瘤细胞中的结缔组织生长因子蛋白水平。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae068

Tomoya Suehiro, Khoja Mouhand Ahmad, Nguyen Truong Duc Hoang, Bingwen Xu, Honoka Komatsu, Komei Kurachi, Hiroki Nikawa, Yuichi Mine, Tohru Matsuki, Katsura Asano, Makiko Fujii

{"title":"Activation of platelet-derived growth factor receptors regulate connective tissue growth factor protein levels via the AKT pathway in malignant mesothelioma cells.","authors":"Tomoya Suehiro, Khoja Mouhand Ahmad, Nguyen Truong Duc Hoang, Bingwen Xu, Honoka Komatsu, Komei Kurachi, Hiroki Nikawa, Yuichi Mine, Tohru Matsuki, Katsura Asano, Makiko Fujii","doi":"10.1093/jb/mvae068","DOIUrl":"10.1093/jb/mvae068","url":null,"abstract":"The incidence of malignant mesothelioma (MM), a disease linked to refractory asbestos exposure, continues to increase globally and remains largely resistant to various treatments. Our previous studies have identified a strong correlation between connective tissue growth factor (CTGF) protein expression and MM malignancy, underscoring the importance of understanding CTGF regulation in MM cells. In this study, we demonstrate for the first time that stimulation with platelet-derived growth factor receptor (PDGFR) ligand, PDGF-BB, increases CTGF protein expression levels without affecting CTGF mRNA levels. Inhibition of PDGFR resulted in a reduction of CTGF protein expression, indicating that PDGFR activation is essential in regulating CTGF protein expression in MM cells. PDGF-BB also activated the protein kinase B (AKT) pathway, and inhibition of AKT phosphorylation abolished the PDGFR-induced CTGF protein expression, suggesting that PDGFR acts upstream of CTGF via the AKT pathway. This reinforces the role of CTGF protein as a key regulator of MM malignancy. Additionally, PDGFR activation led to the phosphorylation of mTOR and 4E-BP1, critical regulators of protein synthesis downstream of AKT, suggesting that PDGFR controls CTGF protein expression through the regulation of CTGF mRNA translation.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"460-471"},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intracellular biliverdin dynamics during ferroptosis. 铁蛋白沉积过程中细胞内胆绿素的动态变化

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae067

Kazuma Nakajima, Hironari Nishizawa, Guan Chen, Shunichi Tsuge, Mie Yamanaka, Machi Kiyohara, Riko Irikura, Mitsuyo Matsumoto, Kozo Tanaka, Rei Narikawa, Kazuhiko Igarashi

{"title":"Intracellular biliverdin dynamics during ferroptosis.","authors":"Kazuma Nakajima, Hironari Nishizawa, Guan Chen, Shunichi Tsuge, Mie Yamanaka, Machi Kiyohara, Riko Irikura, Mitsuyo Matsumoto, Kozo Tanaka, Rei Narikawa, Kazuhiko Igarashi","doi":"10.1093/jb/mvae067","DOIUrl":"10.1093/jb/mvae067","url":null,"abstract":"Ferroptosis is a cell death mechanism mediated by iron-dependent lipid peroxidation. Although ferroptosis has garnered attention as a cancer-suppressing mechanism, there are still limited markers available for identifying ferroptotic cells or assessing their sensitivity to ferroptosis. The study focused on biliverdin, an endogenous reducing substance in cells, and examined the dynamics of intracellular biliverdin during ferroptosis using a biliverdin-binding cyanobacteriochrome. It was found that intracellular biliverdin decreases during ferroptosis and that this decrease is specific to ferroptosis amongst different forms of cell death. Furthermore, the feasibility of predicting sensitivity to ferroptosis by measuring intracellular biliverdin was demonstrated using a ferroptosis model induced by the re-expression of the transcription factor BACH1. These findings provide further insight into ferroptosis research and are expected to contribute to the development of cancer therapies that exploit ferroptosis.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"472-483"},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11638335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The 2024 JB Reviewer Award. 2024年JB评论家奖。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae076

引用次数: 0

Thanking All Peer Reviewers. 感谢所有同行评审。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae077

引用次数: 0

Transglutaminase mediates the hardening of fish egg envelope produced by duplication of factor XIIIA gene during the evolution of Teleostei. 转谷氨酰胺酶在鱼类进化过程中介导了因子 XIIIA 基因复制产生的鱼卵包膜硬化。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-12-02 DOI: 10.1093/jb/mvae062

Shigeki Yasumasu, Miyuki Horie, Mayuko Horie, Kodai Sakuma, Chihiro Sato, Hikari Sato, Taiki Nakajima, Tatsuki Nagasawa, Mari Kawaguchi, Ichiro Iuchi

{"title":"Transglutaminase mediates the hardening of fish egg envelope produced by duplication of factor XIIIA gene during the evolution of Teleostei.","authors":"Shigeki Yasumasu, Miyuki Horie, Mayuko Horie, Kodai Sakuma, Chihiro Sato, Hikari Sato, Taiki Nakajima, Tatsuki Nagasawa, Mari Kawaguchi, Ichiro Iuchi","doi":"10.1093/jb/mvae062","DOIUrl":"10.1093/jb/mvae062","url":null,"abstract":"During the fertilization of fish eggs, the hardening of the egg envelope is mediated by transglutaminase (hTGase). After fertilization, TGase undergoes processing. We isolated hTGase from extracts of unfertilized and water-activated rainbow trout eggs. Rainbow trout hTGase (Rt-hTGase) appeared as an 80 kDa protein, and its processed form was 55 kDa. Their N-terminal amino acid sequences were nearly identical, suggesting processing in the C-terminal region. The specific activities were not significantly different, indicating that C-terminal processing does not activate the enzyme itself. We cloned the cDNA by reverse transcription polymerase chain reaction (RT-PCR) using degenerate primers followed by RACE-PCR. The deduced amino acid sequence of the cDNA was similar to that of factor XIII subunit A (FXIIIA). Molecular phylogenetic and gene syntenic analyses clearly showed that hTGase was produced by duplication of FXIIIA during the evolution to Teleostei. The 55 kDa processed form of Rt-hTGase is predominantly composed of an enzyme domain predicted from the amino acid sequence of the cDNA. It is hypothesized that the C-terminal domain of Rt-hTGase binds to egg envelope proteins, and that processing allows the enzyme to move freely within the egg envelope, increasing substrate-enzyme interaction and thereby accelerating hardening.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"427-436"},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular senescence: mechanisms and relevance to cancer and aging. 细胞衰老：机制及其与癌症和衰老的关系。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-16 DOI: 10.1093/jb/mvae079

Shota Yamauchi, Akiko Takahashi

引用次数: 0

Correction to: The Hox-based positional memory in muscle stem cells. 更正：肌肉干细胞中基于 Hox 的位置记忆。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae066

引用次数: 0

Ciliary length variations impact cilia-mediated signaling and biological responses. 纤毛长度的变化会影响纤毛介导的信号传递和生物反应。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae057

Yuki Kobayashi, Akie Hamamoto, Yumiko Saito

{"title":"Ciliary length variations impact cilia-mediated signaling and biological responses.","authors":"Yuki Kobayashi, Akie Hamamoto, Yumiko Saito","doi":"10.1093/jb/mvae057","DOIUrl":"10.1093/jb/mvae057","url":null,"abstract":"Primary cilia are thin hair-like organelles that protrude from the surface of most mammalian cells. They act as specialized cell antennas that can vary widely in response to specific stimuli. However, the effect of changes in cilia length on cellular signaling and behavior remains unclear. Therefore, we aimed to characterize the elongated primary cilia induced by different chemical agents, lithium chloride (LiCl), cobalt chloride (CoCl2) and rotenone, using human retinal pigmented epithelial 1 (hRPE1) cells expressing ciliary G protein-coupled receptor (GPCR), melanin-concentrating hormone (MCH) receptor 1 (MCHR1). MCH induces cilia shortening mainly via MCHR1-mediated Akt phosphorylation. Therefore, we verified the proper functioning of the MCH-MCHR1 axis in elongated cilia. Although MCH shortened cilia that were elongated by LiCl and rotenone, it did not shorten CoCl2-induced elongated cilia, which exhibited lesser Akt phosphorylation. Furthermore, serum readdition was found to delay cilia shortening in CoCl2-induced elongated cilia. In contrast, rotenone-induced elongated cilia rapidly shortened via a chopping mechanism at the tip of the cilia. Conclusively, we found that each chemical exerted different effects on ciliary GPCR signaling and serum-mediated ciliary structure dynamics in cells with elongated cilia. These results provide a basis for understanding the functional consequences of changes in ciliary length.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"369-383"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of APBB1 as a substrate for anaplastic lymphoma kinase. 鉴定 APPB1 作为无性淋巴瘤激酶的底物。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae055

Yuji Suzuki, Shoma Tsubota, Kenji Kadomatsu, Kazuma Sakamoto

{"title":"Identification of APBB1 as a substrate for anaplastic lymphoma kinase.","authors":"Yuji Suzuki, Shoma Tsubota, Kenji Kadomatsu, Kazuma Sakamoto","doi":"10.1093/jb/mvae055","DOIUrl":"10.1093/jb/mvae055","url":null,"abstract":"Anaplastic lymphoma kinase (ALK) is a well-known oncogene involved in various malignancies such as anaplastic large cell lymphoma, lung cancer and neuroblastoma. Several substrates for fused ALK have been identified and their biological functions have been described. However, the lack of a comprehensive identification of ALK substrates limits our understanding of the biological roles of receptor ALK. Thus, this study aimed to identify novel ALK substrates and characterize their biological functions. We screened the interactors of the kinase domain of receptor ALK using proximity-dependent biotin identification and identified 43 interactors. We narrowed down the candidates by evaluating whether these interactors were downstream of ALK in a neuroblastoma cell line, NB-1. Amongst these, we identified amyloid beta precursor protein-binding family B member 1 (APBB1) as an ALK downstream molecule involved in NB-1 cell viability. Finally, we assessed the kinase-substrate relationship between ALK and APBB1 and found that ALK phosphorylated multiple tyrosine residues in APBB1 both in-cell and in-tube assays, with tyrosine 269 as a major target. In conclusion, we successfully identified a new substrate for receptor ALK. Our results may help further elucidate the molecular mechanism of ALK downstream signalling in neuroblastoma.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"395-403"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0