Journal of biochemistry最新文献_第5页

Biochemical characterizations of the central fragment of human Reelin and identification of amino acid residues involved in its secretion. 人类瑞林中心片段的生化特征以及参与其分泌的氨基酸残基的鉴定。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae058

Takao Kohno, Ikuma Nakagawa, Airi Taniguchi, Fang Heng, Mitsuharu Hattori

引用次数: 0

Dietary methionine functions in proliferative zone maintenance and egg production via sams-1 in Caenorhabditis elegans. 膳食蛋氨酸通过 sams-1 在秀丽隐杆线虫的增殖区维持和产卵过程中发挥作用。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae054

Keiko Hirota, Rieko Yamauchi, Mai Miyata, Mariko Kojima, Koichiro Kako, Akiyoshi Fukamizu

{"title":"Dietary methionine functions in proliferative zone maintenance and egg production via sams-1 in Caenorhabditis elegans.","authors":"Keiko Hirota, Rieko Yamauchi, Mai Miyata, Mariko Kojima, Koichiro Kako, Akiyoshi Fukamizu","doi":"10.1093/jb/mvae054","DOIUrl":"10.1093/jb/mvae054","url":null,"abstract":"The maintenance of germ cells is critical for the prosperity of offspring. The amount of food consumption is known to be closely related to reproduction, i.e. the number of eggs decreases under calorie-restricted conditions in various organisms. Previous studies in Caenorhabditis elegans have reported that calorie restriction reduces the number of eggs and the reduction can be rescued by methionine. However, the effect of methionine on the reproductive process has not been fully understood. In this study, to assess the gonadal function of methionine metabolism, we firstly demonstrated that a depletion in dietary methionine resulted in reduced levels of S-adenosyl-l-methionine (SAM) and S-adenosyl homocysteine in wild-type N2, but not in glp-1 mutants, which possess only a few germ cells. Second, we found no recovery in egg numbers upon methionine administration in SAM synthase (sams)-1 mutants. Furthermore, a reduced number of proliferative zone nuclei exhibited in the sams-1 mutants was not rescued via methionine. Thus, our results have shown that dietary methionine is required for the normal establishment of both the germline progenitor pool and fecundity, mediated by sams-1.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"359-367"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141751760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations associated with neurodevelopmental disorders affect ARF1 function and cortical development. 与神经发育障碍有关的变异会影响 ARF1 的功能和大脑皮层的发育。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-11-04 DOI: 10.1093/jb/mvae053

Tomoki Ishiguro, Mariko Noda, Masashi Nishikawa, Koh-Ichi Nagata, Hidenori Ito

{"title":"Variations associated with neurodevelopmental disorders affect ARF1 function and cortical development.","authors":"Tomoki Ishiguro, Mariko Noda, Masashi Nishikawa, Koh-Ichi Nagata, Hidenori Ito","doi":"10.1093/jb/mvae053","DOIUrl":"10.1093/jb/mvae053","url":null,"abstract":"ADP-ribosylation factors (ARFs) are a family of small GTPases that regulate vesicle trafficking and actin dynamics in cells. Recent genetic analyses have revealed associations between variations in ARF genes and neurodevelopmental disorders, although their pathophysiological significance remains unclear. In this study, we conducted biochemical, cell biological and in vivo analyses of ARF1 variants linked to neurodevelopmental disorders. The mant-GDP dissociation assay revealed that ARF1-p.R19C, -p.F51L, -p.R99C and -p.R99H exhibit higher GDP/GTP exchange activity compared to ARF1 wild type (WT). The GTPase-activating protein (GAP) increased the GTPase activity of WT, p.R19C, p.Y35H, p.F51L, p.P131L and p.P131R, but not of p.Y35D, p.T48I, p.R99C and p.R99H. The transient expression of p.R99C, p.R99H and p.K127E in mammalian cells resulted in the disruption of the Golgi apparatus. In utero electroporation-mediated gene transfer into the cortical neurons of embryonic mice demonstrated that p.R99C, p.R99H and p.K127E cause a migration defect. Expression of these variants resulted in the expansion of the Golgi apparatus in migrating cortical neurons. These findings suggest that the ARF1 variants linked to neurodevelopmental disorders, specifically p.R99C, p.R99H and p.K127E, disrupt the structure of the Golgi apparatus, thereby leading to a developmental defect of cortical neurons.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"347-357"},"PeriodicalIF":2.1,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular mechanisms of mechanosensing and plasticity of tendons and ligaments. "肌腱和韧带机械感应和可塑性的分子机制"。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae039

Takahide Matsushima, Asahara Hiroshi

引用次数: 0

Dyslipidemia and hyperglycemia induce overexpression of Syndecan-3 in erythrocytes and modulate erythrocyte adhesion. 血脂异常和高血糖会诱导红细胞中的Syndecan-3过度表达，并调节红细胞的粘附性。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae050

Smitha Honnalagere Mallanna, Rajesh K Thimmulappa, Nandini D Chilkunda

{"title":"Dyslipidemia and hyperglycemia induce overexpression of Syndecan-3 in erythrocytes and modulate erythrocyte adhesion.","authors":"Smitha Honnalagere Mallanna, Rajesh K Thimmulappa, Nandini D Chilkunda","doi":"10.1093/jb/mvae050","DOIUrl":"10.1093/jb/mvae050","url":null,"abstract":"Erythrocytes are important vascular components that play vital roles in maintaining vascular homeostasis, in addition to carrying oxygen. Previously, we reported that the changes in the internal milieu (e.g. hyperglycemia or hypercholesterolemia) increase erythrocyte adhesion to various extracellular matrix components, potentially through altering glycosaminoglycans (GAGs). In this study, we have investigated the expression of syndecan (Sdc) family members that could be involved in mediating cytoadherence under conditions of dyslipidemia and hyperglycemia. Among the Sdc family members analysed, we found significant overexpression of Sdc-3 in erythrocyte membranes harvested from high-fat-fed control and diabetic animals. Animal studies revealed a positive correlation between Sdc-3 expression, blood sugar levels and erythrocyte adhesion. In the human study, diabetic cohorts with body mass index >24.9 showed significantly increased expression of Sdc-3. Interestingly, blocking the Sdc-3 moiety with an anti-Sdc-3 antibody revealed that the core protein might not be directly involved in erythrocyte adhesion to fibronectin despite the GAGs bringing about adhesion. Lastly, Nano liquid chromatography-mass spectrometry/MS verified the presence of Sdc-3 in erythrocyte membranes. In conclusion, the high-fat diet and diabetes modulated Sdc-3 expression in the erythrocyte membrane, which may alter its adhesive properties and promote vascular complications.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"289-298"},"PeriodicalIF":2.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatial heterogeneity and functional zonation of living tissues and organs in situ. 活体组织和器官在原位的空间异质性和功能分区。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae049

Yu Miyamoto, Masaru Ishii

引用次数: 0

CRABP2 promotes cell migration and invasion by activating PI3K/AKT and MAPK signalling pathways via upregulating LAMB3 in prostate cancer. CRABP2 在前列腺癌中通过上调 LAMB3 激活 PI3K/AKT 和 MAPK 信号通路，从而促进细胞迁移和侵袭。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae052

Rui Wang, Zhaoping Liao, Chunhua Liu, Shifang Yu, Kaihua Xiang, Ting Wu, Jie Feng, Senjuan Ding, Tingao Yu, Gang Cheng, Sanlian Li

{"title":"CRABP2 promotes cell migration and invasion by activating PI3K/AKT and MAPK signalling pathways via upregulating LAMB3 in prostate cancer.","authors":"Rui Wang, Zhaoping Liao, Chunhua Liu, Shifang Yu, Kaihua Xiang, Ting Wu, Jie Feng, Senjuan Ding, Tingao Yu, Gang Cheng, Sanlian Li","doi":"10.1093/jb/mvae052","DOIUrl":"10.1093/jb/mvae052","url":null,"abstract":"Prostate cancer (PCa) has become a worldwide health burden among men. Previous studies have suggested that cellular retinoic acid binding protein 2 (CRABP2) significantly affects the regulation of cell proliferation, motility and apoptosis in multiple cancers; however, the effect of CRABP2 on PCa is poorly reported. CRABP2 expression in different PCa cell lines and its effect on different cellular functions varied. While CRABP2 promotes cell migration and invasion, it appears to inhibit cell proliferation specifically in PC-3 cells. However, the proliferation of DU145 and 22RV1 cells did not appear to be significantly affected by CRABP2. Additionally, CRABP2 had no influence on the cell cycle distribution of PCa cells. The RNA-seq assay showed that overexpressing CRABP2 upregulated laminin subunit beta-3 (LAMB3) mRNA expression, and the enrichment analyses revealed that the differentially expressed genes were enriched in the phosphoinositide 3-kinase (PI3K)/activated protein kinase B (AKT) and mitogen-activated protein kinase (MAPK) signalling pathways. The following western blot experiments also confirmed the upregulated LAMB3 protein level and the activation of the PI3K/AKT and MAPK signalling pathways. Moreover, overexpressing CRABP2 significantly inhibited tumour growth in vivo. In conclusion, CRABP2 facilitates cell migration and invasion by activating PI3K/AKT and MAPK signalling pathways through upregulating LAMB3 in PCa.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"313-324"},"PeriodicalIF":2.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141748309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Hox-based positional memory in muscle stem cells. 肌肉干细胞中基于 Hox 的位置记忆。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae059

Ryosuke Okino, Yuki Goda, Yusuke Ono

引用次数: 0

Cutting-edge skin ageing research on tissue stem cell. 关于组织干细胞的尖端皮肤老化研究。

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae022

Ryo Ichijo

引用次数: 0

Cardiac remodeling: novel pathophysiological mechanisms and therapeutic strategies. 心脏重塑：新的病理生理学机制和治疗策略

IF 2.1 4区生物学

Journal of biochemistry Pub Date : 2024-09-30 DOI: 10.1093/jb/mvae031

Motohiro Nishida, Xinya Mi, Yukina Ishii, Yuri Kato, Akiyuki Nishimura

{"title":"Cardiac remodeling: novel pathophysiological mechanisms and therapeutic strategies.","authors":"Motohiro Nishida, Xinya Mi, Yukina Ishii, Yuri Kato, Akiyuki Nishimura","doi":"10.1093/jb/mvae031","DOIUrl":"10.1093/jb/mvae031","url":null,"abstract":"Morphological and structural remodeling of the heart, including cardiac hypertrophy and fibrosis, has been considered as a therapeutic target for heart failure for approximately three decades. Groundbreaking heart failure medications demonstrating reverse remodeling effects have contributed significantly to medical advancements. However, nearly 50% of heart failure patients still exhibit drug resistance, posing a challenge to the healthcare system. Recently, characteristics of heart failure resistant to ARBs and β-blockers have been defined, highlighting preserved systolic function despite impaired diastolic function, leading to the classification of heart failure with preserved ejection fraction (HFpEF). The pathogenesis and aetiology of HFpEF may be related to metabolic abnormalities, as evidenced by its mimicry through endothelial dysfunction and excessive intake of high-fat diets. Our recent findings indicate a significant involvement of mitochondrial hyper-fission in the progression of heart failure. This mitochondrial pathological remodeling is associated with redox imbalance, especially hydrogen sulphide accumulation due to abnormal electron leak in myocardium. In this review, we also introduce a novel therapeutic strategy for heart failure from the current perspective of mitochondrial redox-metabolic remodeling.","PeriodicalId":15234,"journal":{"name":"Journal of biochemistry","volume":" ","pages":"255-262"},"PeriodicalIF":2.1,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140174872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0